유산균 발효액의 항균특성에 관한 연구

구자경,조남준 한국기술교육대학교 2006 論文集 Vol.13 No.1

The antimicrobial properties of fermentation products of lactic acid bacteria such as L. mesenteroides, L. citreum and P. pentosaceus have been investigated with using the minimum inhibitory concentration (MIC) method and the challenge method. The fermentation products of lactic acid bacteria contain bacteriocins and organic acids that show antimicrobial activities. The antimicrobial properties of metabolic products produced from three lactic acid bacteria were compared with that of the synthetic antiseptics such as methylparaben and triclosan. The antimicrobial substances produced from L. mesenteroides, L. citreum and P. pentosaceus have shown strong antimicrobial activities against P. aerugionosa, and B. subtilis, E. coli, S. aureus. Their antimicrobial activities are similar or superior to that of methylparaben or triclosan. However, their antimicrobial activities were relatively weak against C. albicans compared with that of the synthetic antiseptics, methylparaben or triclosan. The antimicrobial substances produced from L. mesenteroides, L. citreum and P. pentosaceus could be promising preservatives for cosmetics, pharmaceutical products and foods in future.

O/W 마이크로에멀젼과 나노에멀젼의 특성에 관한 연구

조남준,구자경 한국기술교육대학교 2006 論文集 Vol.13 No.1

We have investigated the properties of oil-in-water microemulsions and nanoemulsions. The nanoemulsions are prepared with using a microfluidizer under 1,500bar and they showed decreasing droplet diameter upon repeating cycles in fluidizer up to six times. The microemulsions prepared with using a homogenizer exhibited the decrease in particle diameter with increasing surfactant HLB value, decreasing oil content and increasing surfactant/oil ratio. However, the diameters of nanoemulsions prepared with using a fluidizer, a high shear device, did not much depend on the HLB of surfactants and the oil content in water. The particle size of nanoemulsions prepared with 10% soybean oil converged around 110-125nm regardless of surfactant HLB values.

水稻作 耕地의 區劃規模에 따른 農業機械의 作業性能(Ⅱ) : 乾畓直播 栽培 Direct-Seeding in dry Land

유수남,구자웅 全南大學校 農業科學技術硏究所 1997 農業科學技術硏究 Vol.32 No.-

본 연구는 건답직파 수도재배의 경우 농업기계 이용측면에서 포장의 대구획화에 따른 경지정리의 적정 구획규모 결정을 위한 기초자료와 영농의 효율화와 생산성 향상을 위한 영농방법 및 이에 따른 기계화 작업체계 수립의 기초자료를 제공하고자 경지구획(경구)의 규모와 형상에 따른 대형 고성능 농업기계의 포장작업능률, 포장작업효율 등을 분석하였으며, 그 연구결과를 요약하면 다음과 같다. 1. 장변 길이가 길어짐에 따라 이앙작업을 제외한 모든 작업에서 포장작업능률과 포장작업효율은 높아지는 것으로 나타났으며, 이에 따라 ha당 총작업소요시간도 줄어들었다. 2. 단변의 길이 증가에 따른 포장작업능률과 포장작업효율은 구동경운, 정지, 파종작업 등에서는 약간씩 증가하였으며, 수확작업에서는 거의 일정하게 나타난 반면, 제초제 살포와 병충해약제 살포 등 방제작업과 추비살포작업에서는 약간씩 낮아지는 것으로 나타났다. 한편 시비작업(기비살포)에서는 단변의 길이 변화에 따라 크게 변동하였다. ha당 총작업소요시간은 대체적으로 방제작업의 비중이 상대적으로 크기 때문에 단변의 길이 증가에 따라 약간씩 감소하는 것으로 나타났다. 3. 포장작업능률은 거의 모든 작업에서 동일한 구획규모에서는 장단변비의 증가에 따라 대체적으로 증가하였으며, 또한 같은 장단변비에서는 구획규모가 클수록 증가하는 것으로 나타났다. 총작업소요시간은 동일한 구획에서는 장단변비가 커질수록 감소하며, 같은 장단변비에서는 구획규모가 클수록 적어짐을 보였다. 그러나 장단변비가 커질수록 포장작업능률 증가율과 총작업소요시간의 감소율은 떨어지는 것으로 나타났다. 4. 대구획화를 위하여 장변의 길이를 길게하는 것은 단변을 길게하는 것보다 거의 모든 작업에서 효과적으로 포장작업능률과 포장작업효율을 높일수 있는 것으로 나타났다. 현행 농업기계 체계를 고려해 볼 때 장변의 길이는 100∼150 m, 단변의 길이는 60∼80 m로 각각 제한되어 적정 구획규모는 약 0.6∼1.2 ha 정도인 것으로 판단되며, 포장의 배수성 개선, 대형 트랙터에 적합한 부착작업기의 채용,포장주행이 가능한 방제 및 추비살포기계의 개발이 이루어진다면 농업기계능률을 높이기 위하여 1.2∼1.6 ha의 구획규모로 경지를 대구획화하는 것이 바람직 할 것으로 보인다. This study was carried out to evaluate the effect of field size on field capacities of farm machines and their operating times for determining the optimum field size in direct-seeding of rice in dry land. As long side length increases, the field capacities and field efficiencies increased and total operating time per ha of the machines decreased in most works. As short side length increases, the field capacities and field efficiencies increased slightly in rotary tilling, pulverizing, and seeding works, and were almost constant in harvesting work. But those were changed irregularly due to the wide effective working width in fertilizing work, and were decreased slightly owing to the time loss for resting in weedicide and pesticide application works. Total operating time per ha of the machines decreased slightly as the short side length increases. As the ratio of long side length to short side length increases under same field size, the field capacities of the machines increased and total operating time per ha of the machines decreased. But, increasing rates of field capacities and decreasing rate of operating time per ha were reduced gradually. In the case of same ratio, field capacities increased and total operating time per ha decreased as the field size increases. Increase of the long side length is more effective than that of the short side length for improving the field capacities of machines and reducing their operating times. Long side length is limited by the loading capacities of chemical distributers and short side length is limited by the effective working width of machines. And therefore long side and short side can be lengthende up to 100 - 150 m and 60 - 80 m, respectively and optimum field size is in the range of 0.6 - 1.2 ha under the current farm machine system. But, if improvement of drainage, and development of chemical distributer which can run in the field are achieved, field size in the range of 1.2 - 1.6 ha is desirable for improving field capacities of machines.

Thiobacillus 박테리아의 흡착에 대한 온도 및 혼합배양의 영향

조남준,구자경 한국기술교육대학교 2001 論文集 Vol.8 No.1

The adsorptions of Thiobacillus ferrooxidans, Thiobacillus thiooxidans and their mixed cultures onto the copper-containing furnace dust were examined in an acidic medium solution. Adsorption of T. ferrooxidans and T. thiooxidans fits well to Langmuir isotherm. The maximum adsorption amount, XAm and adsorption equilibrium constant, KA gradually increase as the temperature increases from 5 ℃ to 35 ℃. The X_(Am) value of the mixed culture is larger than that of any single culture but slightly smaller than the sum of each bacterial component value, which indicates that these two bacteria have both of some preferential and competitive adsorption sites on furnace dust. The adsorption affinity, K_A of mixed culture is about 4.5 or 3.4 times larger than that of T. thiooxidans or T. ferrooxidans, respectively, which suggests that the synergistic effect exists in their bindings.

1980年度 痢疾樣 大便을 가진 患兒들에 對한 臨床的 細菌學的 觀察

趙龍勳,南聖枝,具滋薰,安斗洪,薛盛用 慶北大學校 醫科大學 1980 慶北醫大誌 Vol.21 No.2

1980年 1月부터 1980年 8月까지 滿 8個月間 痢疾樣 大便을 主訴로 本 小兒科에서 入院및 來院한 患兒 75名을 對象으로 臨床的, 細菌學的 觀察을 하여 다음과 같은 咸績을 얻었다. 年齡別 分布는 1歲에서 5歲 사이가 35例(47%)로 가장 높았으며 이들中 Shigella는 23例(30%), Entameba histolytica는 13例(17%)였고 Vibrio parahaemolyticus는 한 例도 檢出되지 않았다. 檢出된 Shigella 23菌株는 모두 Shigella flexneri 였다. 月別分布는 6月 16例(20%), 7月 30例(40%)로서 最多數를 占하였으나 細菌性 痢疾의 경우 月別에 관계없이 均等하게 分布하고 있었다. 臨床症狀및 理學的 所見으로는 細菌性痢疾의 경우 38℃ 以上의 發熱 19例(83%), 嘔吐 6例(26%), 腹痛 8例(35%), 痙攣 5例(22%)였으며, 아메바性 痢疾에서는 發熱 4例(31%), 痙攣 1例(8%)로서 細菌性 痢疾에서 아메바性 痢疾보다 發熱과 痙攣이 빈번히 나타났다. 泄瀉의 性狀에서는 細菌性 痢疾 23例中 血粘液性이 19例(83%)였는데 비해 아메바性 痢疾 13例에서는 粘液性이 8例(62%)였다. 泄瀉의 回數에서도 細菌性 痢疾에서는 대부분이 11回以上이었는데 比해 아메바性 痢疾에서는 대부분이 10回未滿이었다. 末梢血液에서의 白血球數는 全例의 35%에서 白血球 增多症을 보였다. 分離된 Shigella 23菌株에 對한 抗生劑 感受性 檢査를 平板混合포稀釋法에 依해 調査해 본 結果 kanamycin, gentamicin, amikacin, cephaloridine, rifampin이 100%의 感受性을 나타내어 가장 높았고, nalidixic acid에서도 87%의 感愛性을 나타냈으며, sulfonamide, chloramphenicol, streptomycin, tetracycline, ampicillin, bactrim 에서는 한 菌株를 제외한 모든 菌株에서 耐性을 나타냈다. 그리고 이들 Shigella 23菌株中 1菌株를 제외한 나머지 모든 菌株에서 6種 以上의 抗生物質에 對해 多抗生物質耐性을 보여 주었다. A clinical and laboratory study was conducted on 75 children with dysentery-like stool, who were admitted or visited to our pediatric department during 8 months period from January to August 1980. The following results were obtained: One to five years of age group was affected most frequently (47%). Shigella flexneri was identified by stool culture in 23 cases (30%) and Entameba hitolytica in 13 cases (17%). In bacillary dysentery group, fever was the most common symptom occuring in 83%, followed by abdominal pain in 35%, vomiting in 26% and convulsion in 22%. In amoebic dysentery group, fever was noted in 31% and convulsion in 8%, showing some contrast to the frequency of fever and CNS manifestation. About the character of diarrheal stools, 83% of bacillary dysentery group showed bloody, mucoid stool and 62% of amoebic dysentery group mucoid ones. Frequency of diarrhea was 11 times per day or more in 73% of bacillary dysentery patients, and 10 times per day or less in 92% of amoebic dysentery patients. The result of sensitivity test of isolated Shigella to various antibiotics were as follow: 100% sensitive to kanamicin, gentamicin, amikacin, cephaloridine and rifampin. 87% to nalidixic acid, and 4.3% to sulfonamide, chloramphenicol, streptomycin, tetracyclin, ampicillin and bactrim. And 22 out of 23 isolated Shigella strains revealed multiplydrug-resistance patterns to 6 or more antibiotics.

위암에서의 Transforming Growth Factor-B의 발현에 대한 연구

구자영(Ja Young Koo),공덕경(Deuk Kyung Kong),박종남(Jeong Nam Park),박선자(Seon Ja Park),정숙금(Seuk Kum Chung),허만하(Man Ha Huh) 대한소화기학회 1996 대한소화기학회지 Vol.28 No.3

N/A Background/Aims: Transforming growth factor-g(TGF-g), mutifanctional cytokine, has been suggested to have many actions related to tumor progression and tumor cell behavior, because of its increased expression in cancer cells. Only small number of studies were done for its role in gastric carcinoma, and we have conducted this study to elucidate in depth the role of TGF-/3 in gastric carcinoma progression. Methods: Expression of TGF-/3, epidermal growth factor(EGF), p53 and proliferating cell nuc1ear antigen(PCNA) in gastric carcinoma was studied by immunohi- stochemical method applied to paraffin-embedded tissue sections of endoscopic biopsy materials of 71 cases of gastric carcinoma(24 early and 47 advanced) and imrnunoreactivity of antigens was correlated with histological differentintion of carcinoma, degree of tumor infiltration of mononu- clear cells, serum levels of alphafetoprotein(AFP) and carcinoembryonic antigen(CEA), and pre- sence of distant metastases. Results: TGF-0 in carcinoma tissue was expressed in 31 cases(43.7%) of total 71 cases, and immunopositivity of TGF-0 in advanced gastric carcinoma(AGCj was 55.3%, which was much higher than that(20.8%) of early gastric carcinoma(EGC)(pC0.05). 1here was no significant difference in imrnunopositivity of TGF-0 between different differentiation group. There was negative correlation between expression of TGF-g and degree of tumor infiltration of mono- nuclear cells(p0.05). Immunopositivity of TGF-0 in AGC group was significantly higher in the cases with elevated serum AFP level(82.6%) than that with normal serum AFP leve1(29.1%)(p. 0.05), and also was significantly higher in the cases with elevated serum CEA level(87.6%) than that with normal serum CEA level(40.6%)(pw0.05). There was no correlation between expression of TGF-g and presence of distant metastases or metastatic sites. There was significant positive correlation between expression of TGF-g and EGF(p=0.004), TGF-g and p53(p=0.02) but no correlation between expression of TGF-g and PCNA. Conclusions: Above data indicate that TGF-f3 rnay contributes to gastric carcinoma progression by immunosuppressive action and fibrosis, by growth-stimulatory action via EGF modulation, and that loss of growth-inhibitory effects of TGF-0 on gastric carcinoma, which is related to p53 mutation, may be a critical factor in gastric carcinoma progression. (Korean J Gastroenterol 1996;28:336 - 348)

혈청 CA-125, 초음파 및 양전자방출단층촬영술을 통한 골반 종물의 감별진단

구자성 ( Ja Seong Koo ),김영태 ( Young Tae Kim ),김성훈 ( Sung Hoon Kim ),김재욱 ( Jae Wook Kim ),김재훈 ( Jae Hoon Kim ),박용원 ( Yong Won Park ),김복자 ( Bok Ja Kim ),남은지 ( Eun Ji Nam ),윤미진 ( Mi Jin Yun ) 대한산부인과학회 2005 Obstetrics & Gynecology Science Vol.48 No.8

참외흰가루병에 대한 나노화 오일 혼합제제의 방제효과

구자준(Ja-Jun Koo),김현수(Hyun-Soo Kim),조현호(Hyun-Ho Cho),남준호(Jun-Ho Nam),남기웅(Ki-Woong Nam) 한국원예학회 2014 한국원예학회 학술발표요지 Vol.2014 No.10

Representation of a Conceptual Design for a Rectilinear Motion Polymer Actuator

Ja Choon Koo,Kwang Mok Jung,Jong Kil Park,Jae Do Nam,Young Kwan Lee,Jae Wook Jeon,Hyouk Ryeol Choi 대한전기학회 2007 International Journal of Control, Automation, and Vol.5 No.4

A number of different alternative actuation methods have been under active development for some specific applications where the traditional electromechanical actuators are difficult to apply. Recently, many of these substitutes are trying to employ new smart materials like electroactive polymers. However most of the polymeric materials are flexible and vulnerable so that they normally can not sustain external forces. Although the materials have shown a good potential to be used for alternative actuation mechanisms, no tangible industrial application is yet presented because of the reason. A conceptual design for a rectilinear motion actuator using dielectric elastomer is presented in this article. The introduced design concept might enable to produce fairly controllable rectilinear motions for various applications and the presented prototype actuator system is fully packaged in a small unit and controlled by a standard communication interface.

후복막강내 발생한 Castleman병 1례

남성진,이동욱,박찬규,정시욱,김추성,조성래,구본천,양사규,이일웅,박정자 慶北大學校 醫科大學 1997 慶北醫大誌 Vol.38 No.2

Castleman' s disease is a rare, reactive lymphoid proliferation of unknown cause and pathogenesis. Depending on histololgic findings, it has been divided into two distinct histologic subtypes that include hyaline-vascular type(90%) and plasmacell type(10%). This disease occurs most commonly in mediastinal lymph nodes and cervical lymph nodes, inguinal lymph nodes respectively. But occurs rarely in retroperitoneum. We report a case of hyaline-vascular type asymptomatic Castleman' s disease of the retroperitoneum in a 47-year-old man.