간내 담석과 동반된 간내 담도암에서의 p53과 Ki-ras의 발현

김명환,이성구,서동완,이승규,남승우,박능화,민영일,김연석,심기남,공경엽 대한소화기학회 1997 대한소화기학회지 Vol.30 No.5

Background/Aims: Hepatolithiasis or primary intrahepatic stones may be accompanied later by intrahepatic cholangiocarcinoma. This cancerous lesion is frequently associated with atypical hyperplastic epithelium that was suspected of being precancerous. To investigate the Ki-ras or p53 mutation may play a role in carcinogenesis, and to determine whether atypical hyperplastic epithelium may be precancerous, this study was performed in intrahepatic cholangiocarcinomas associated with hepatolithiasis. Methods: We examined 12 patients with intrahepatic cholangiocarcinomas associated with hepatolithiasis. Ki-ras point mutations were assessed by direct DNA sequencing. Expressions of p53 protein were immunohistochemically assessed. The overexpression of p53 and point mutation of Ki-ras were examined in normal, hyperplastic, atypical hyperplastic, and can- cerous tissues separately. Results: The expressions of p53 protein were detected in 4 (33%) of 12 cholangiocarcinomas and Ki-ras point mutations at codon 12 were found in 2 (17%) of 12 cholangiocarcinomas. In those two carcinoma cases which contained the mutant sequence of Ki-ras, the same point mutation from wild type GGT (glycine) to GAT (aspartic acid) was. found in the associated atypical hyperplastic epithelium. However, none of the normal and hyperplastic epithelium harbored Ki-ras or p53 mutations. Conclusions: The overexpression of p53 may play a part in the carcinogenesis of some intrahepatic cholangiocarcinomas associated with hepatolithiasis, whereas the role of Ki-ras mutations in those cases is dubious. However, Ki-ras point mutation at codon 12 may be responsible for either cancer or atypical hyperplastic epithelium associated with hepatolithiasis in certain cases, suggesting atypical hyperplastic epithelium may give rise to carcinoma.

직장 카르시노이드 종양에서 Ki-67 발현의 예후적 중요성

홍수민 ( Su Min Hong ),김유선 ( You Sun Kim ),문정섭 ( Jung Seop Moon ),김진남 ( Jin Nam Kim ),오명기 ( Myoung Ki Oh ),권선옥 ( Sun Ok Kwon ),정성연 ( Seong Yeon Jeong ),홍성우 ( Seong Woo Hong ),강윤경 ( Yun Kyung Kang ) 대한소화기학회 2013 대한소화기학회지 Vol.61 No.2

Background/Aims: Rectal carcinoid tumors can be resected with endoscopy, and it is important to assess their prognostic factors. We evaluated the potential of Ki-67 expression as a prognostic factor in rectal carcinoid tumors. Methods: We retrospectively reviewed the medical records of 37 patients with rectal carcinoid tumors who got endoscopic resection from January 2001 to January 2011 at Inje University Seoul Paik Hospital. We analyzed their endoscopic and histologic findings, Ki-67 expression, clinical outcome, and prognosis. Results: The mean age (±SD) of the patients was 56.3±10.7 years, and the male : female ratio was 3.6:1. The mean tumor size was 0.5±0.4 cm, 33 patients showed grade 1 tumors (89.2%) and the average Ki-67 expression was 0.7±1.2%. Thirty five patients underwent endoscopic mucosal resection, and two required endoscopic submucosal dissection. Eight patients had positive margins after resection, but no cases of lymphovascular invasion were identified. The median follow-up duration was 21.4±25.4 months, and no recurrences were observed. Conclusions: In low grade rectal carcinoid tumors which are lack of central depression on colonoscopy, the expression of a molecular marker of malignant potential, Ki-67, was low. Therefore, endoscopic resection seemed to be a safe and effective treatment for these tumors.

STS316L 모재 및 용착부의 열처리에 따른 초음파 특성

남기우,박소순,안석환 대한금속재료학회 2003 대한금속·재료학회지 Vol.41 No.3

The degradation characteristics of the base metal and weld of STS316L were investigated by the experimental method of ultrasonic exposure with parameters such as ultrasonic velocity, attenuation factor and frequency. Through this research, degradation characteristics could be evaluated by the combinations of experimental ultrasonic parameters. According to the increase of grain sizes, standard deviation and ferrite contents, the ultrasonic velocities and frequencies were decreased, while the ultrasonic attenuation factors were increased. This results can be to predict the welded structures life time assesmant according to the aged degradation and damage without disjointing or stopping in-service.

Rohrer지수에 의한 한국과 중국 연변지역 조선족 아동의 체격비교

남은우,박기만,배성권 韓國學校保健學會 1996 韓國學校保健學會誌 Vol.9 No.1

This paper was performed to comparative study with the children's body strength status of Ethinc Korean in China and Korea. The subjects of this study were the students of each elementary school located in a rural and a urban areas in China. To compared the elvel of their body strength status, we investigated to the students of 2 elementary schools as comparative group on Kojae Area in Korea. We used student's physical record in the school. The contents of the study were the body weight, the chest circumstance, and the body height of the subjects. To make out the level of the students' physique, we measured by Rohrer Index output through above body height and body weight of them. The results of this were as follows; 1. In the case of Ethinc Korean in China, the average body weight(kilogram) is 28.94±6.68 in male 28.51±7.58 in famels. Comparatively, the average body weight in the case Korean is 31.99±7.23 in male and 31.74 ±7.82 in female. The average body height of Ethnic Korean is inferior about 2~5 Kilograms to that of Korean. 2. The average chest circumstance(centimeter) is 63.51±5.21 in male 63.05±5.87 in female of Ethinc Korean, 65.54±6.43 in male and 64.57±7.81 in female of Korean. Compared to between two group, the average chest circumstance of Ethinc Korean is inferior about 2~4 centimeters to that of Korean. 3. The average body height(centimeter) of Ethinc Korean is 135.28±8.96 in male 134.82±9.74 in female, and that of Korean is 136.68±9.12 in male and 136.10±9.92 in female. Like the preceding below results, the average body height of Ethinc Korean is inferior about 2~5 centimeters to that of Korean. 4. Compared to the fullness degree of physique between two group using Rohrer Index, Ethinc Korean is thiner than Korean. 5. Temporary, in case of children from 10 to 12 years old, female is superior to male through the index contained of the body weight, the chest circumstance, and the body height. It seems that female and male had different maximum growth age.

제2형 당뇨병 남성 환자에서 유리 테스토스테론과 성호르몬 결합 글로블린 농도

남기덕,김영설,박철영,오승준,김덕윤,우정택,김성운,양인명,김진우,최영길 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.24 No.6

연구배경:인슐린저항성은 제2형 당뇨병과 심혈관질환의 주요한 위험 인자로 성호르몬과 상호 관계가 있다고 알려져 있다. 그러나 여성과는 달리 남성에서는 연령과 인슐린저항성에 따른 유리 테스토스테론과 성호르몬 결합 글로블린 농도의 변화에 대한 연구가 부족한 실정이다. 그러므로, 본 저자등은 제2형 당뇨병 남성 환자에서 정산인과 비교해서 유리 테스토스테론과 성호르몬 결합 글로블린 농도를 측정하고 연령에 따른 변화 정도를 알아보고자 하였다. 방법:대상 환자 모두에서 연령과 체질량지수, 총 콜레스테롤, 중성지방, 공복혈당과 인슐린 농도를 측정하였다. 혈중 유리 테스토스테론 농도는 방사면역측정법(radioimmunoassay)을 이용해서 측정하였고, 혈중 성호르몬 결합글로불린은 면역방사계측측정법(immunoradiometric assay)을 이용해서 측정하였다(Diagnostic System Laoratories, Wbster, TX, USA). 결과:1)제2형 당뇨병 남성 환자에서 정상 대조군 남성에 비해 성호르몬 결합글로불린은 104.1±35.0 vs 25.7±3.5 mole×10??로 의미 있게 높았으나(p<0.001), 유리 테스토스테론은 13.7±9.5 vs 13.6±6.5 ng/dL로 차이가 없었다. 2)연령과 성호르몬 결합 글로블린 사이의 상관 계수는 제2형 당뇨병 남성 환자에서 0.40로 중등고의 양의 상관 관계를 보였고(p<0.001), 정상 대조군 남성에서 0.11로 유의한 상관 관계를 보이지 않았다. 연령과 유리 테스토스테론 사이의 상관 계수는 제2형 당뇨병 남성 환자에서 0.08, 정상 대조군 남성에서-0.17로 모두에서 유의한 상관 관계를 보이지 않았다. 3)연령과 체질량지수를 보정한 후에 제2형 당뇨병 남성 환자와 정상 대조군 남성에서 혈중 인슐린 농도, 유리 테스토스테론과 성호르몬 결합 글로블린 사이에는 상관 관계가 없었다. 결론:제2형 당뇨병 남성 환자에서 정상 남성에 비해 성호르몬 결합 글로블린 농도가 증가되어 있었으며, 유리 테스토스테론은 차이가 없었다. 연령이 증가함에 따라 성호르몬 결합 글로블린이 제2형 당뇨병 남성 환자에서 정상 남성에 비해 증가 폭이 의미있게 컸으며, 유리 테스토스테론은 변화가 없었다. Background: Insulin resistance is a risk factor for cardiovascular disease and type 2 diabetes mellitus. There are many previous studies indicating that insulin lowers serum sex hormone-binding globulin levels, and there is inverse correlation between insulin resistance and serum sex hormone-binding globulin levels in women. However, in men, a limited number of studies are available to explain the effect of sex hormone on age and insulin. Therefore, the present study was undertaken to investigate the relationship among free testosterone, hormone-binding globulin and age in type 2 diabetic men and control subjects. Method: Age, body mass index, total cholesterol, triglyceride, fasting blood sugar, and insulin concentrations were examined on 89 type 2 diabetic men and 47 control subjects. The free testosterone level was measured by commercially available double-antibody system (Radioimmunoassay). The sex hormone-binding globulin level was also measured by commercially available double-antibody system(Immunoradiometric assay). Results: 1) Sex hormone-binding globulin level was significantly increased in patients with type 2 diabetes. However, there was no significantly difference in free testosterone level between the two groups. 2) Sex hormone-binding globulin was positively correlated with age (r=0.4, p <0.001) in patients with type 2 diabetes. Sex hormone-binding globulin and free testosterone were not correlated with age in control sujects. 3) Free testosterone and sex hormone-binding globulin concentrations were not significantly related to serum insulin concentration after adjusting for age and body mass index. Conclusions: We observed increased sex hormone-binding globulin concentration in diabetes man, and was a positively related to age. Further studies are needed to understand the relationships between age, insulin resistance, testosterone, and sex hormone-binding globulin concentrations(J Kor Diabetes Asso 24:699~707,2000).

균열크기 측정기법 개발에 관한 연구

남민우,조찬희,정한섭,김홍덕,신기석 한국비파괴검사학회 2004 학술대회 논문집 Vol.- No.-

본 연구는 증기발생기 건전성 향상기술 개발 과제 일환으로 진행중인 고리 1호기 구 증기발생기 인출 전열관을 대상으로 하는 Round Robin Test의 사전 시험을 위해 수행하였다. 균열결함에 대한 검출 및 크기측정의 기준을 설정하기 위해 SGMP Mock-Up를 제작하였고 신호수집한 Data를 증기발생기 전열관 가동중검사 업무를 수행하고 있는 와전류신호평가자들 4개조가신호평가에 참여하였으며, 그 결과를 요약 정리하였다. 본 연구에서 SGMP Mock-Up Data Crack Sizing의 목적은 고리 1호기 구 증기발생기 전열관을 대상으로 시행될 Round Robin Test 전에 결함 기구별 균열결함의 특성 이해 및 균열결함이 여러 유형의 외부 환경에서 나타나고 있을 때의 균열결함에 대한 정량적인 크기 측정값의 바람직한 모델 적용을 확인하기 위함이다. SGMP Mock-Up에 적용한 와전류검사는 Robbin 및 MRPC 검사를 포함하였다. 본 연구의 주 목적인 MRPC 데이터를 이용한 균열깊이 평가에 2개조는 위상각-깊이 분석법을 적용하여 신호평가를 수행하였고, 2개조는 위상각-깊이 분석법 이외에 진폭-깊이 평가 기법을 적용하여 SGMP Mock-Up에 대한 최적의 기법을 설정하였다.

STS316L 용접부의 피로거동 및 초음파시럼에 의한 열화특성 평가

남기우,박소순,안석환,도재윤,박인덕 한국비파괴검사학회 2003 한국비파괴검사학회지 Vol.23 No.2

STS316L은 인성, 연성, 부식저항성 등 우수한 기계적 성질 때문에 에너지환경설비 등 구조물에 널리 이용되고 있다. 그러나, 용접구조물은 가혹한 환경하에서 경년열화가 심각하다. 지금까지의 연구들은 주로 용접부의 피로균열진전거동에 관하여 수행되었고, 피로균열진전속도식의 재료상수와 비파괴적 평가의 상관성에 관한 연구는 거의 없었다. 본 연구는 STS316L 용접부의 피로균열진전 거동을 조사하고, 초음파 파라미터로 열화특성을 평가하고 시간-주파수 분석으로 해석을 하였다. 본 연구 결과는 용접구조물의 사용기간 중 피로수명 등을 예측하기 위한 기초 데이터로서 사용될 수 있다. STS316L had been used as the structural material for energy environmental facilities, because austenite stainless steels like 316L have superior mechanical properties of which toughness, ductility, corrosion resistant and etc. However, those welded structures are receiving severe damage due to increasing of the aged degradation. Most studies until now have been carried out against fatigue bahaviors of weldments, and were not well studied on nondestructive evaluation methods. In this study, the fatigue crack propagation behavior of STS316L weldment usually used for vessels of the nuclear power plant was investigated. Also, the degradation characterstics of 316L stainless steel weldments were evaluated by the ultrasonic parameter such as ultrasonic velocity, attenuation factor and time-frequency analysis. The results of this study can be used as a basic data for the prediction of the fatigue crack life of weldments structures without disjointing or stopping service of structures in service.

시간-주파수 해석법에 의한 CFRP의 파괴 거동

남기우,안석환,이상기,김현수,문창권 한국비파괴검사학회 2001 한국비파괴검사학회지 Vol.21 No.1

신호처리법으로 현재 많이 사용되고 있는 푸리에 변환은 신호의 주파수 성분이 사간에 따라 어떻게 변화하는지를 표현하지 못한다. 따라서 최근 이와 같은 푸리에 변환의 단점을 보완하여, 신호의 시간과 주파수에 대한 정보를 동시에 표현할 수 있는 시간-주파수 해석법들이 개발되었다. 본 연구에서는 음향방출을 이용하여 복합재료의 주요 발생원으로 알려져 있는 기지균열, 섬유분리, 섬유파괴 및 층간분리 등과 같은 파괴기구를 해석하였다. 각각의 파괴특성이 나타나도록 시험편을 제작하여 인장시험 시 검출된 음향방출신호의 시간-주파수 해석을 통해 전체 파괴기구의 특징을 분석하였다. Fourier transform has been one of the most common tools to study the frequency characteristics of signals. With the Fourier transform alone, however, it is difficult to tell whether signal's frequency contents evolve in time or not. Except for a few special cases, the frequency contents of most signals encountered in the real world change with time. Time-frequency analysis methods are developed recently to overcome the drawbacks of Fourier transform, which can represent the information of signals in time and frequency at the same time. In this study, damage process of cross-ply carbon fiber reinforced plastic (CFRP) under monotonic tensile loading was characterized by acoustic emission. Different kinds of CFRP specimens were used to determine the characteristics of AE signals. Time-frequency analysis methods were employed for the analysis of fracture mechanisms in CFRP such as matrix cracking, debonding, fiber fracture and delamination.

한국의 광물명칭에 관한 연구

정원우,상기남,이윤종,김중욱,곽기근 慶北大學校 科學敎育硏究所 2003 科學敎育硏究誌 Vol.27 No.-

The purpose of this study is to investigate historical records on Korean mineral terms. One of the earliest records on Korean mineral terms is the Donguebogam(oriental medical handbook) written by Heo Jun in 1613. Most of geological investigation of Korea was accomplished by Japanese geologists in the period of the 1900~1945. Korean mineralogical records have been described by Gottsch(1886), Kwasaki(1935), Kinosaki(1937, 1940), Tsuda(1941, 1952) and Galleger(1963). Korean mineral species have been described about 300 species by KIER(Korea Institute Energy and Resources) in 1977. In the results of this study, Comparisons and classifications between current korean mineral terms and korean traditional and chinese medical mineral terms are suggested.

수직 고정망에 고정된 미생물을 이용한 Ethanol 제조

金男紀,李眞雨 成均館大學校 科學技術硏究所 1987 論文集 Vol.38 No.1

Kinetic behaviors of immobilized microbial cells on vertical film by aluminium screen in a column reactor were investigated for batch and continuous fermentation at 30℃ and pH=5.0. For the comparison of ethanol productivity, Zymomonas mobilis ATCC 29191, Saccharomyces cerevisiae ATCC 26603 and dual organisms of these were separately immobilized with 3%(W/V) agar-agar gel and 4%(W/V) k-carrageenan gel. The results obtained in this experiment were as follows : 1) In batch fermentation, the maximum produced ethanol concentration of Z. mobilis was 47.8g/l, that of S. cerevisiae was 37g/l and that of the dual organisms was 50g/l in agar-agar gel. In k-carrageenan gel, the maximum produced ethanol concentration of Z. mobilis was 45g/l, that of S. cerevisiae was 36.3g/l and that of the dual organisms was 47.3g/l. 2) In continuous fermentation, the maximum volumetric ethanol productivity of Z. mobilis was 19.36g/l·hr, that of S. cerevisiae was 18.16g/l·hr and that of the dual organisms was 26.4g/l·hr in agar-agar gel, while in k-carrageenan that of Z. mobilis was 17.09g/l·hr, that of S. cerevisiae was 14.7g/l·hr and that of the dual organisms was 17.82g/l·hr. The volumetric ethanol productivity of the dual organisms was better than that of Z. mobilis and of S. cerevisiae. The agar-agar gel was a more efficient matrix than k-carrageenan gel in ethanol production and volumetric ethanol productivity. 3) The ratio of ethanol concentration at height of 30cm from the bottom of the fermentor to the ethanol concentration at the effluent port was over 90% for each cell and matrix.