Effect of Ionizing Radiation on Rat Tissue: Proteomic and Biochemical Analysis

Park*, Eui‐,Chul,Yoon, Jong‐,Bok,Seong*, Jin‐,Sil,Choi, Kyoung‐,Soo,Kong, Eung‐,Sik,Kim, Yun‐,Jeong,Park, Young‐,Mee,Park, Eun‐,Mi Taylor Francis 2006 Preparative biochemistry & biotechnology Vol.36 No.1

<P>Reactive oxygen species (ROS), generated by ionizing radiation, has been implicated in its effect on living tissues. We confirmed the changes in the oxidative stress markers upon irradiation. We characterized the changes in the proteome profile in rat liver after administering irradiation, and the affected proteins were identified by MALDI-TOF-MS and ESI-MS/MS. The identified proteins represent diverse sets of proteins participating in the cellular metabolism. Our results demonstrated that proteomics analysis is a useful method for characterization of a global proteome change caused by ionizing radiation to unravel the molecular mechanisms involved in the cellular responses to ionizing radiation.</P>

온라인 화상 인증을 위한 분산 객체 활성화 시스템

허진경,양환석,박승규,장근실,김종민 朝鮮大學校 統計硏究所 2007 統計硏究所論文誌 Vol.9 No.1

클라이언트로부터 전송되는 인증 데이터를 실시간으로 서버에서 분석하여 처리하는 시스템의 경우에는 입력되는 데이터의 양에 비례하여 서버의 부담은 증가하게 된다. 이때 서버에서는 데이터의 병목현상이 발생하게 되고, 이는 바로 전체적인 시스템의 성능을 저하시키는 결과를 초래하게 된다. 본 논문에서는 데이터 처리의 병목현상을 해결하여 시스템의 성능을 높일 뿐만 아니라, 인터넷상의 유휴 서버들을 활용할 수 있게 하기 위한 방안으로 분산처리 기술을 이용한 전처리 작업 시스템과 그 성능을 향상시키기 위한 분산 객체 활성화 시스템을 제안한다.

2003년 국내 중증급성호흡기증후군 진료 현황 및 문제점 분석

이진수,김은실,정문현,백제중,정선화,안주희,최영화,이선희,고철우,김성범,김민자,박승철,기현균,송재훈,최상호,김양수,이상오,조용균,박영훈,정숙인,김연숙,이흥범,손창희,장성희,정희진,김우주 대한감염학회 2004 감염과 화학요법 Vol.36 No.3

목적 : 2002년 말 중국에서 SARS가 발생한 이후 국내에서도 2003년 10월까지 총 3명의 추정환자, 17명의 의심환자가 보고되었다. 향후 추가적인 SARS의 유행이 우려되는 상황에서, 그간의 SARS 환자 진료에 있어서의 실질적인 준비사항, 진료 현황 등에 대한 조사를 통해 문제점을 파악하여, 향후 더 나은 대비가 될 수 있도록 개선점을 제시하고자 하였다. 재료 및 방법 : SARS로 의심되는 환자를 진료 경험이 있는 병원의료진을 대상으로 2003년 10월에 설문조사를 실시하였다. 설문에는 SARS 환자 진료 시의 실질적인 조치, 진료 현황, 병실, 응급실 및 외래에서의 격리 시설과 준비사항, 보건당국의 관리와 지원에 관한 사항을 포함하였다. 결과 : 대상이 되는 22개 병원 중 17개(17/22, 77.2%) 병원이 설문에 응하였다. SARS 환자를 위한 격리실은 응급실, 외래, 일반병실 및 중환자실에서 각각 9개(9/17, 52.9%), 5개(5/17, 29.4%), 15개(15/16, 93.7%), 4개(4/16, 25%) 병원에서 음압처리가 되어있지 않은 일인실 혹은 다인실이 사용되었고, 1개(1/16, 6.3%) 병원에서만 일반병실에서 음압격리실이 운영되었다. 입원환자의 진찰 시 개인보호구의 착용은 거의 모든 의료기관에서 이루어졌다. 보건당국에서 SARS지정병원의 시설 등을 사전에 확인한 곳은 1곳(1/12, 8.3%)이였고, 14개 병원(14/15, 93.3%)에서는 보건당국에 의뢰한 검사결과를 통하 받지 못하였다. 결론 : 의료기관에서 SARS 환자용 격리실뿐만 아니라 기존의 격리실 설비 등이 미흡하였으며, 특히 중환자실 및 외래의 준비가 더욱 부족하였다. 보건당국의 의료기관에 대한 종합적인 지원이 부족하였고, 병원과의 원활한 연계가 잘 이루어지지 않았다. SARS 만이 아닌 격리를 필요로 하는 질환의 적절한 진료를 위해 향후 병원 시설의 정비와 정부차원에서의 보다 구체적이고 실질적인 대책마련이 필요하다. Background : There was an worldwide outbreak of the Severe Acute Respiratory Syndrome (SARS) originated from China in late 2002. During that period three cases of suspected SARS and 17 cases of probable SARS were reported in Korea. With the concerns about the reemergence of SARS-coV transmission, it is important to be prepared for any possibility. So, this study is aimed to analysis the past measures in managing SARS and propose the amendatory plans to improve the preparedness. Materials & Methods : Questionnaires were collected among clinicians with any experience in managing the probable or suspected SARS cases in Oct. 2003. 17 out of 22 hospitals responded to the questionnaire. The contents in the questionnaire were practical activities, personal equipments, response plans, isolation facilities in emergency centers, outpatient clinics, general wards and intensive care units, and relationship with the public health department. Results : The dedicated isolation rooms in emergency centers, outpatient clinics, general wards, and intensive care units were prepared in 9 (9/17, 52.9%), 5 (5/17, 29.4%), 15 (15/16, 93.7%), and 4 (4/16, 25.0%) hospitals, respectively. Except for one hospital that newly made negative pressure room for SARS, single or multi-bed rooms without airborne infection control were used in all the other hospitals. The personal precaution principles were kept quite well in general wards. Before the designation of SARS hospital by the public health department prior evalution to see if the hospital was suitable for managing SARS was conducted in only 1 (1/12, 8.3%) hospital. The results of laboratory diagnosis were reported back in 1 (1/15, 6.6%) hospital. Conclusions : The isolation facilities which can control airborne infection were almost deficient not only for SARS but also for other respiratory transmissible diseases. For the infection control of transmissible diseases including SARS, more investment is needed on medical facilities and comprehensive support from the public health department required.

Orientia tsutsugamoshi에 대한 항균제 병용의 실험실 효과

김은실,김미경,이혜명,정문현,이진수,강재승 대한감염학회 2008 감염과 화학요법 Vol.40 No.6

Background : Occasionally, combinations of antibiotics are used for the treatment of scrub typhus. However, the effectiveness of such combined therapies has rarely been evaluated. To date, no experimental studies have been performed; only 1 clinical study has assessed the efficacy of combined doxycycline and rifampin therapy. To elucidate the efficacies of other antibiotic combinations, we performed an experiment to evaluate the in vitro efficacy of antibiotic combinations against Orientia tsutsugamushi. Materials and Methods : O. tsutsugamushi strain Boryong was inoculated into the ECV304 cell line. The infected cells were cultured in antibiotic-containing media for 3-5 days and stained with FS15, a monoclonal antibody reacting against the linear epitope on the 56-kDa major outer membrane protein of O. tsutsugamushi. Thereafter, antimicrobial susceptibility was measured by flow cytometry and expressed as a growth index (total mass of Orientia). The growth indices of doxycycline (0.1 ug/mL), azithromycin (0.1 ug/mL), rifampin (0.0125 ug/mL), cefotaxime (2 and 20 ug/mL), and their various combinations (doxycycline+cefotaxime, doxycycline+rifampin, azithromycin+cefotaxime, and rifampin+cefotaxime) were measured. The above mentioned antibiotic concentrations, except for that of cefotaxime, represent the minimal inhibitory concentrations of each antibiotic. Results : The growth indices of doxycycline (4.67% and 0.52%), rifampin (2.35% and 0.26%), and azithromycin (7.54%) were within the range of full suppression of O. tsutsugamushi', in contrast, cefotaxime (87.60%) was in effective. The growth indices of doxycycline + rifampin were 0.10% and 0.10% which were similar to those obtained with doxycycline or rifampin alone. The growth indices of doxycycline+cefotaxime were 3.99% and 3.65% in low-dose cefotaxime (2 ug/mL), and 3.69% and 4.40% in high-dose cefotaxime (20 ug/mL). The growth indices of rifampin +cefotaxime (2.19% and 2.19% at 2 ug/mL; 1.84% and 2.04% at 20 ug/mL cefotaxime) were similar to those obtained with rifampin alone (2.35% and 0.26%). Azithromycin+cefotaxime (11.06-14.63%) showed higher growth indices than azithromycin alone; this suggests that this combination may be antagonistic. Conclusions : The anti-Orientia efficacies of doxycycline+rifampin, doxycycline+cefotaxime, and rifampin+cefotaxime were not antagonistic. The efficacy of the azithromycin+cefotaxime combination needs to be confirmed by more sensitive methods to exclude the possibility of antagonistic interactions between the antibiotics.

유세포분석법으로 조사한 Orientia tsutsugamushi Boryong주의 항균제 감수성

김은실,김미경,이혜명,정문현,이진수,박재은,강재승 대한감염학회 2008 감염과 화학요법 Vol.40 No.4

Background : Scrub typhus, an infectious disease caused by Orientia tsutsugamushi, is endemic in Korea. With the introduction of tetracycline and chloramphenicol in clinical practice, the mortality due to scrub typhus has markedly decreased, In 1995, scrub typhus poorly responsive to doxycycline was reported in Thailand; the need for safe antibiotics for the treatment of scrub typhus acquired during pregnancy or for children is emerging; also, broader spectrum antibiotics having anti-Orientia activity may be preferred for empirical therapy of enteric fever syndrome and for complicated scrub typhus. The anti-Orientia activities of various antibiotics, including recently licensed antibiotics, were investigated by flow cytometry. Materials and Methods : O. tsutsugamushi strain Boryong was inoculated into the ECV304 cell line. The infected cells were stained with FS15, a monoclonal antibody reacting against a linear epitope on 56-kDa major outer membrane protein of 0. tsutsugamushi. Then the antimicrobial susceptibilities were measured by flow cytometry and expressed as a growth index (total mass of Orientia). A concentration at which no further decrease in growth index occurred was defined as the minimal inhibitory concentration (MIC). Microbial susceptibilities to the following antibiotics were measured: quinupristin-dalfopristin (Synercid), levofloxacin, ciprofloxacin, moxifloxacin, metronidazole, linezolid, clindamycin, chloramphenicol, doxycycline, azithromycin, and rifampin. Results : Considering the usual serum concentrations of rifampin (M1C=0.025-0.05 ug/mL), azithromycin (M1C=0.05-0.5 ug/mL) and doxycycline (MIC=0.05-0.1 ug/mL), these antibiotics exhibited very low MICs. Synercid (MIC=0.2-1.0 ug/mL), clindamycin (MIC=1.0 ug/mL) and chloramphenicol (MIC=1-2 ug/mL) exhibited moderately low MICs; moxifloxacin (MIC=8 ug/mL), ciprofloxacin (M1C=25.6 ug/mL or more) and levofloxacin (M1C=30 ug/mL) exhibited relatively high MICs; and cefotaxime (MIC>50 ug/mL), metronidazole (MIC>30 ug/mL) and linezolid (>30 ug/mL) exhibited high MICs. Conclusions : Among the new antibiotics, none was superior to doxycycline, azithromycin or rifampin with respect to anti-Orientia activity. Synercid, clindamycin, and moxifloxacin may show moderate therapeutic efficacies in human.

Orientia tsutsugamushi 감염에서 doxycycline 용량과 투여기간에 대한 실험

김은실,김미경,이혜명,정문현,이진수,박재은,강재승 대한감염학회 2008 감염과 화학요법 Vol.40 No.5

Background : Doxycycline, azithromycin, and chloramphenicol have been used for the treatment of scrub typhus; however, the duration of treatment for this disease is still controversial. Most clinical studies on the treatment of scrub typhus focus on the effectiveness of antibiotics; few studies have focused on the duration of the treatment. There has been no in vitro evaluation of the optimal duration of treatment of scrub typhus. We performed an experiment to evaluate the regrowth of Orientia tsutsugamushi after exposure to doxycycline of various concentration and exposure time. Materials and Methods : O. tsutsugamushi strain Boryong was inoculated into the ECV304 cell line. The infected cells were stained with FS15, a monoclonal antibody reacting against a linear epitope on the 56-kDa major outer membrane protein of O. tsutsugamushi; thereafter, the antimicrobial susceptibilities were measured by flow cytometry and expressed as a growth index (total mass of Orientia). Incubation in media containing 0.1 (the minimal inhibitory concentration of doxycycline against O. tsutsugamushi strain Boryong), 0.2, and 2 ug/mL doxycycline for 1, 3, 5, and 7 day was followed by incubation in antibiotic-free media for 5 day. Two inocula sizes were employed; low inoculum (45.18% of cells were infected) and high inoculum (87.21% of cells). Results : The incubation with 0.1 ug/mL doxycycline resulted in O. tsutsugamushi regrowth irrespective of doxycycline treatment duration. When the concentration of doxycycline was increased to 0.2 or 2 ug/mL, regrowth was observed in short duration treatments (1-5 day at 0.2 ug/mL; 1 day at 2 ug/mL). Higher inoculum size of O. tsutsugamushi increased regrowth (1 day at the low inoculum; 1-5 day at the high inoculum). Conclusions : The regrowth of O. tsutsugamushi after doxycycline treatment depends on the doxycycline dose; the higher the dose, the lower the relapse rate. If this result is extrapolated to humans, higher dose of doxycycline might be preferred to lower the relapse rate. The inoculum effect in O. tsutsugamushi infection needs further investigations.

중합효소연쇄반응으로 조사한 관절염 환자의 관절액에서 Tropheryma whipplei DNA 검출

김성연,정문현,최새로운,김은실,이진수,박원,김미경,강재승 대한감염학회 2005 감염과 화학요법 Vol.37 No.2

목적 : Whipple병은 Tropheryma whipplei에 의한 전신적인 감염 질환으로 소장과 관절 침범이 특징이며, 흔히 병의 초기에는 수년 동안 다른 증상 없이 관절염 소견만 보이므로, 일부 환자는 염증성 관절염으로 진단받을 가능성이 있다. 또한 류마티스 관절염을 포함하여 여러 염증성 관절염에서는 아직 병인이 밝혀지지 않았지만 감염이 관련될 가능성은 꾸준히 언급이 되었다. 저자들은 국내에서도 T. whipplei가 존재할 것이라는 가정 하에 한국인에서 중합효소연쇄반응으로 T. whipplei DNA를 조사하였다. 재료 및 방법 : 대상 환자군은 1997년부터 2002년 사이에 인하대병원 류마티스 내과에 관절 종창으로 내원한 환자들로, 류마티스 관절염 환자 33명, 골관절염 환자 18명, 기타 질환에서 관절 이상인 환자들로 5명이 포함되었다. 건강한 성인 8명의 타액과 22명의 위내시경을 받은 환자의 위액을 채취하여 검사하였다. 중합효소연쇄반응은 T. whipplei heat shock protein의 일부 유전자를 증폭할 수 있는 2개의 시동체를 사용하였다. 표준 균주를 얻기가 어려워 양성 대조군 없이 중합효소연쇄반응을 시행하였다. 결과 : 관절염 환자의 관절액, 정상인의 타액, 위내시경을 받은 사람의 위액에서 모두 중합효소 연쇄반응으로 핵산을 증명할 수 없었다. 결론 : 양성대조군이 없어 검사 잘못일 가능성도 배제할 수 없으나, Whipple병이 염증성 관절염의 흔한 원인이 아니거나 관절의 직접 침범보다는 위장관 감염 후 생기는 면역 반응에 의한 반응성 관절염일 가능성이 있다. 관절액 이외의 검체에서 조사한다면 다른 결과를 얻을 수도 있으리라 생각하며 앞으로 이들에 대한 조사가 필요하다. Background : Whipple's disease is a chronic systemic infection caused by Tropheryma whipplei which chiefly involves gastrointestinal tract and joints. Because arthritis is often the first sign of Whipple's disease, Whipple's disease may be misdiagnosed in the early stage of illness as non-infectious inflammatory arthritis. Rheumatoid arthritis may also be caused by infectious agents, including T. whipplei. To elucidate the relationship between T. whipplei infection and various arthritis, we examined T. whipplei DNA in Korean patients by polymerase chain reaction (PCR). Materials and Methods : With two T. whipplei-specific primers dervied from the partial heat shock protein 65 gene sequence, PCR was performed on 56 joint fluid samples : 33 samples from RA patients, 18 samples from OA patients, and 5 samples from patients with other unflammatory arthritis. In addition, the same method was applied to find out the DNA of T. whipplei in 8 normal adults' saliva and 22 patients' gastric juice. Due to the difficulty in obtaining the reference strain of T. whipplei, the PCR was performed without the positive control. Results : The PCR method did not reveal T. whipplei in joint fluid from 56 patients with arthropathies, 8 samples from saliva, and 22 samples from gastric juice. Conclusion : Though technical errors can not be excluded as a cause of absence of a positive case, our results suggest that T. whipplei infection may not be a common cause of various arthritis in Korea; T. whipplei may be associated with inflammatory arthritis indirectly by immune-mediated mechanism rather than by direct joint invasion. The examination of specimens other than joint fluid could be helpful and the study of larger number of patient is highly desirable.

브루셀라균에 노출된 미생물 검사실 직원들에 대한 예방적 항생제 요법과 혈청학적 추적검사

이창섭,이혜수,이재현,박진희,최영실,황규잠,이흥범 대한감염학회 2008 감염과 화학요법 Vol.40 No.2

The aerozolization is one of possible Brucella transmission mechanisms, particularly in air-borne exposed laboratory workers. In this study, seven laboratory workers were potentially exposed to B. abortus via aerosols. Two laboratory workers who sniffed an agar plate several times were considered to be at high risk for acquiring the disease, 5 workers who did not work directly with the strain were be considered at low risk of infection. Prophylactic antibiotics of doxycycline 200 mg/day and rifampin 600 mg/day were offered for 6 weeks in high risk workers and 3 weeks for low risk workers respectively. Enrolled workers were advised to check for serological testing of Brucella species every 3 weeks during a total period of 12 weeks. Compliance with taking medicine was 57.1% (4/7) and compliance for completing the serological tests was 85.7% (6/7). None of the laboratory workers developed clinical disease or tested positive serologically during 3 months of seroloaical testing and 1 year of clinical follow-up.

Bacillus sp. FF-7에 의한 항산화물질 생산조건과 항산화 활성

차재영,김효정,전방실,박진철,옥민,조영수 한국응용생명화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.3

발효식품 시료로부터 분리된 세균을 DPPH(α,α¹-diphenyl-β-picrylhydrazyl) 전자공여능으로 항산화 활성을 측정하여 가장 활성이 강한 균주를 선별하여 형태학적, 생화학적, 생리학적 특성 및 16S rRNA 염기서열을 조사한 결과 Bacillus sp.으로 판명되어 FF-7로 명명하였다. DPPH 전자공여능법에 의한 Bacillus sp. FF-7이 생산하는 항산화물질의 최적 생산 배지조건은 탄소원 2% galactose와 질소원 1% tryptone 첨가였다. Bacillus sp. FF-7에 의해 생성된 항산화 물질의 활성을 DPPH 전자공여능, 흰쥐 각 조직 microsomal 실험계 및 linoleic acid 과 산화지질 실험계에서 malondialdehyde를 thiobarbituric acid(TBA)방법으로 측정하였다. 흰쥐 각 조직 microsomal 실험계에서 지질과산화에 대한 항산화 효과는 뇌(97.50%)>심장(79.95%)>신장(77.84%)>비장(77.47%)>고환(69.96%)>간장(62.45%) 순이였다. Linoleic acid의 과산화지질률 TBA법으로 측정한 결과 반응 4일째까지 억제 효과가 강하게 나타났으며, 동시에 대조구로 사용한 0.05% BHT 첨가구에서도 실험종료시까지 항산화 활성이 강하게 나타났다. The antioxidative activity of antioxidative substances produced from several bacterial strains isolated from fermented foods were tested by DPPH (α,α'-diphenyl-β-picrylhydrazyl) free radical scavenging activity. One of the strains showing the highest antioxidative activity was identified as Bacillus sp. based on the morphological, biochemical, physiological characteristics, and 165 rRNA sequence, and named FF-7. The molt optimal medium condition for the production of antioxidative substance from Bacillus sp. FF-7 was 2% galactose as carbon source and 1% tryptone as nitrogen source. The antioxidative substance produced from FF-7 in these cultural medium was also tested by in vitro experimental models, the peroxidation of linoleic acid and the peroxidation of rat tissues microsomes by using thiobarbituric acid (TBA) for assay of free rnalondialdehyde production. The antioxidative avtivity against lipid peroxidation of rat tissues microsomes was shown in the following order; brain 97.50% > heart 79.95% > kidney 77.84% > spleen 77.47% > testis 69.96% > liver 62.45%. The antioxidative substance produced from FF-7 on linoleic acid peroxidation by TBA method was effectively inhibited during four days, and 0.05% BHT(butylated hydroxytoluene) used comparative control was also effectively inhibited. Results showed that the highest antioxidative activity by DPPH method of antioxidative substance produced from Bacillus sp. FF-7 was obtained by supplementing 2% galactose as carbon source and 1% tryptone as nitrogen source in cultured medium, this substance effectively inhibited the formation of TBARS in brain microsome in vitro system and in linoleic acid peroxidation.

손가락 끝 점을 이용한 손 형상 인식

김종민,이웅기,장근실,양환석,허진경,박승규 朝鮮大學校 統計硏究所 2007 統計硏究所論文誌 Vol.9 No.1

최근 사용자 친화형 유저 인터페이스 구현을 위해 인간의 손 형상을 실시간으로 인식하는 연구의 중요성이 부각되고 있다. 그러나 인간의 손은 자유도가 크기 때문에 손 형상을 정확히 인식하기란 매우 어렵고 또한 피부색과 유사한 색을 가지는 복잡한 배경에서는 더욱 곤란하다. 본 논문에서는 별도의 센서를 부착하지 않고 카메라를 사용하여 피부색 정보에 의한 손 형상을 분할한 후 손가락 끝 점을 찾는다. 찾은 손가락 끝 점을 이용하여 방향을 탐지하는 알고리즘에 대해 기술한다. 이 방법은 템플리트 매칭을 이용하여 손가락 끝 점을 탐색한 후 찾은 손가락 끝 점과 손목의 중심을 이용하여 전, 후, 좌, 우 방향을 탐지한다. 제안하는 방법을 이용하여 3D 가상현실 공간에서의 네비게이션(Navigation)에 응용하였으며, 실험결과 전진, 후진 및 좌측, 우측의 방향전환도 매우 좋은 결과를 보였다. 또한 본 논문에서 제안하는 방법은 마우스, 키보드, 조이스틱 등의 조작 없이 전, 후, 좌, 우 방향전환을 사용자가 직관적으로 지시함으로써 보다 자연스러운 인간과 컴퓨터의 상호작용을 제공할 수 있을 것이다.