RAPD 분석에 의한 굴참나무 집단의 유전변이 연구

송정호,김남수,이용섭,김영중,송재모,이재선 한국유전학회 2002 Genes & Genomics Vol.24 No.2

Quercus variabilis is one of the most important tree species in both utilization of forest resources and management of forest ecosystem in Korea. To obtain the basic information for the establishment of breeding strategy and efficient management of genetic resources, five populations of Q. variabilis from 89 selected plus trees were studied using POPGENE and AMOVA analysis based on DNA markers focussing on the genetic diversity and variation among populations. The overall polymorphic amplicons were 78.6% and levels of genetic diversity within 5 populations were similar each other (Shannon's Index : 0.289∼0.423 [mean 0.366]). The values of total genetic diversity (H_t) and average gene diversity within population (H_S) were 0.265 and 0.239, respectively. The degree of genetic differentiation (G_st : 0.098) was relatively high compared with those of other tree species. Based on the cluster analysis of UPGMA, genetic relationships among 5 populations were not coincided with the pattern of geographic distribution.

特用資源植物의 硏究 : 한국임학회지에 게재된 논문을 중심으로 Based on the Articles Published in the Journal of Korean Forestry

이재선,김철우,배찬호,송재모,강효진,황석인,문흥규 江原大學校 森林科學硏究所 2003 Journal of Forest Science Vol.19 No.-

한국임학회지 (1962년의 1호~2002년의 91권 6호)에 게재된 특용자원식물 관련 논문에서 식·음용식물, 약용식물, 사료식물, 조경식물, 섬유식물, 산업용식물 및 밀원식물로 분류하고 그 내용에 따라 입지환경, 생태, 생리, 번식, 조림(또는 무육, 재배), 유전·육종, 분류(또는 식별), 병·충해와 방제, 동물피해와 방제, 성분분석, 식생정보, 생물공학, 경영·경제 및 총설로 나누었다. 총 1,434편의 발표 논문 중 유용식물자원을 대상으로 한 논문은 27.6%를 차지하는 396편이었고, 내용은 식생정보 60편(15.2%), 생리 56편(14.1%), 유전·육종 56편(14.1%), 번식 53편(13.4%) 및 생태 37편(9.3%)이었다. 조림(무육·재배)은 11편(2.8%)으로 특용자원식물의 규모경영이 이루어지지 않고 있음을 시사하고 있으며, 이것은 경영·경제 분야가 6편(1.5%)인 것과 맥을 같이 하고 있었다. 연구대상 식물은 잣나무(42편), 아까시나무(23편), 밤나무(14편) 및 은행나무(14편) 순으로 높았고, 이 식물들과 관련한 연구내용은 번식, 생리, 유전·육종, 생태 및 병·충해 순이었다. 연구의 목적, 내용, 결과 및 대상식물 등을 고려하여 보면, 1) 초본식물에 대한 보다 많은 연구, 2) 타산업 또는 학분 분야와의 공동연구, 3) 자원 보전적 연구 및 4) 연구주체자 간의 연구결과의 공유 등이 매우 요망된다. The articles, published in the Journal of Korean Forestry from Number 1(1962) to number 6, Volume 91(2002), were surveyed and investigated for the research trend analysis about resource-plants for special use or purpose, i.e edible plants, medicinal plants, feed resource, landscape plants, fiber plants, industrial usage, and bee plants. If the purpose or subject matter of the research was construction or furniture timber production, mushrooms and/or pulp and Paper, such research was not included in this study. These articles were classified again depending on the content of research into 14 categories : habitat environment, ecology, physiology, propagation, silviculture(tending and culture), genetics and breeding, identification, insect and disease control, animal-related research, cemponent analysis, vegetation survey, biotechnology, management, and review. Among the total 1,434 articles published, 396 ones (27,6%) were related with plants for special use or purpose. Vegetation survey was 60(15.2%) ; physiology 56(04.1%) ; genetics and breeding 56(14.1%) ; propagation 53(13.4%) ; and ecology 37(9.3%). Siviculture research field included 11 articles (2.8%), which indicates that the management of resource-plants is to far from economic income as seen in the low number of management research filed articles, i. e., only 6 reports(1.5%). Korean white Pine was most popular for research and included 42 articles ; Robinia pseudoacacia 23 Castanea crenata 14 : and ginkgo tree 14, Research related with these species had focused mainly or propagation, physiotogy, genetics and breeding, ecology and pest control. Based on this survey and analysis, the followings are suggested : 1. More research is required on forest herbaceous plants. 2. Cooperative research work with other industrial and/or scientific area is recommendable for commercialization including medicine, cosmetics, and food etc. 3. Research resource-plant conservation. which includes biology, social education and policy, should be supported for next generation. 4. Mutual correspondence and information exchange about the research results between researchers and institutes is more necessary than now.

四物湯이 腹腔 Macrophage 의 貪食能에 미치는 影響

全熏,殷載淳,宋正模 대한본초학회 1999 大韓本草學會誌 Vol.14 No.2

Samultang(SMT) consists of Rehmanniae Radix Preparata, Paeoniae Radix Alba, Cnidii Rhizoma and Angelicae Gigantis Radix. SMT enhanced the lucigenin chemiluminescence and the engulfment of fluorescein-conjugated E. coli particles and did not affect the production of nitric oxide in murine peritoneal macrophage. SMT enhanced the production of γ-interferon, interleukin-2 and the cell viability in murine thymocyte, but did not affect the production of interleukin-4. These results indicate that SMT enhances the phagocytosis of macrophage via the stimulation of γ-interferon production in T_H1 cells.

Trehalose의 노화 억제 효과

박정욱,정한모,송재철 울산대학교 2000 생활과학논문집 Vol.2 No.1

시차주사열량기와 색도계를 이용하여 Trehalose의 가래떡 노화에 미치는 영향을 검토하였다. 가래떡의 노화정도는 저장온도와 무관하게 경시적으로 증가하였는데 Trehalose가 노화를 억제하는 것으로 나타났다. 특히 저온에서 노화가 실온보다 심하게 일어났으며 저장초기에 저장온도에 대한 영향이 큰 것으로 나타났다. 그리고 L*값은 실온저장(25℃)시 냉장저장(4℃)의 경우보다 시간에 따른 변화가 컸으며, 냉동보관시 a*값은 저장 1일째 까지는 변화가 없다가 5%를 첨가한 가래떡을 실온에서 저장한 경우에는 가장 낮은 a*값을 나타내었다. b*값의 경우에는 Trehalose 첨가량과 저장온도에 따라 크게 변하지 않았다. 색깔안정화의 효과는 Trehalose 첨가농도가 증가할수록 큰 것으로 나타났으며, 특히 냉장보관시에 효과가 두드러지는 것으로 관찰되었다. This studies were investigated to provide the influence of trehalose on retrogradation of Korean rice cake by differential scanning calorimeter and tristimulus values of CIE color Croma Meter. Having no connection with storage temperature, degree of retrogradation of Korean rice cake was increased with storage time. Degree of retrogradation of Korean rice cake was decreased in case of addition of trehalose to the Korean rice cake paste. Degree of retrogradation of Korean rice cake stored at low temperature(4℃) was higher than that of Korean rice cake stored at room temperature(25℃). In particular, degree of retrogradation of Korean rice cake was shown to be clearly influenced by storage temperature at the beginning of storage. The value of L of Korean rice cake at room temperature was indicated to be significantly changed compared with that of Korean rice cake at low temperature. The value of a of Korean rice cake was not changed until one day of storage, and shown to be minimized in storage at room temperature(25℃) in case of addition of 5% trehalose to the paste of Korean rice cake. The change of b value in general was not significantly observed even if storage temperature and amount of trehalose have been variable. However effects of trehalose in color stability of Korean rice cake was remarkably exhibited in storage at low temperature(4℃).

사비의 교정에 있어서 내시경의 이용

박대환,김태모,이재욱,송철홍,한동길,안기영 大韓成形外科學會 1998 Archives of Plastic Surgery Vol.25 No.1

Owing to the complexity of the deviated structures and the septal deformities accompanying the external nasal deformities, the complete correction of the deviated nose is regarded as a difficult one. The approach to the nasal bone and osteotomy in classic corrective rhinoplasty is almost blind technique, where the results depends on the feeling by surgeon's hand. To overcome these drawback, endoscopic-assisted corrective rhinoplasty and septoplasty performed for 8 cases of deviated nose between January 1996 and May 1997. Average follow-up period was 10 months. All patients were evaluated by symmetrical nasal pyramid, recurrence of the bony deflection and septal deviation. The postoperative courses were satisfactory in most of cases with few complications. It appears that endoscopic control during corrective rhinoplasty and septoplasty is a big step toward obtaining better results in bony and cartilage resection with extreme precision under monitor control and magnification. This technique is not an open approach but permits one to see more of the nasal skeleton and bony septum what is causing deformity, and the immediate effect of the corrective measures used. The use of endoscope in corrective rhinoplasty will provide expanded field of vision, direct manipulation of lesions, and better aesthetic an6 functional results.

十全大補湯이 抗癌劑를 投與한 생쥐의 胸腺細胞에 미치는 影響

金大根,殷載淳,全薰,宋正模 대한본초학회 1998 大韓本草學會誌 Vol.13 No.2

The purpose of this research was to investigate effects of Sipjeon-Daebo-Tang(SDT) on thymocytes of anti-tumor drugs administered mice. DNA fragmentation, mitochondrial transmembrane potential and subpopulation were tested using a flow cytometry. and the cell viability was tested using a MTT assay. Etoposide, vincristine and doxorubicin enhanced DNA fragmentation and reduced mitochondrial transmembrane potential of thymocytes, but SDT recoverd the action of vincristine and doxorubicin. Etoposide or vincristine decreased the population of CD4 single positive cell in thymocytes, but SDT enhanced the population of CD4+ single positive cell. Etoposide, vincristine and doxorubicin decreased the cell viability of thymocytes, but SDT enhanced the cell viability. These results suggest that SDT has a regulative function of thymocytes in anti-tumor drugs administered mice.

가토에서 일측 요관 부분 및 완전폐색시 신장과 요관의 조직학적 변화

김상현,김용웅,육승모,한동석,노안식,임재성,김홍식,나용길,설종구 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

This study was designed to clarify histologic changes in the experimental rabbit kidney and ureter after unilateral partial or complete ureteral obstruction. A total of 20 adult white rabbits were divided into 4 subgroups in the partial obstruction followed by 2 weeks, the partial obstruction followed by 4 weeks, the complete obstruction followed by 2 weeks, and the complete obstruction followed by 4 weeks. The normal control group was consisted of 5 adult white rabbits. The kidneys and ureters for light microscopy were fixed in 10% neutral formalin, embedded in paraffin and stained with hematoxylin and eosin. Abnormal histologic fingings showed in ipsilateral kidney and ureter of all subgroups(such as dilatation of uriniferous tubule, focal necrosis and desquamation of epithelium, interstitial fibrosis, glomerular congestion, widening of Bowman's space, dilatation of ureter, epithelial cell atrophy or loss, interstitial fibrosis and interstitial inflammation). These findings were most severe in the group of the complete-obstructed ureter for 4 weeks. No significant changes showed in contralateral ureter of all subgroups. The degree of obstruction was severe and period of obstruction was long, the histological changes in ipsilateral side were severe. Despite of changes in ipsilateral side no abnormal findings showed in contralateral side. We suggest that long term experimental investigation of histologic changes in ipsilateral side and compensatory changes in contralateral side have to be studied.

Growing Three Wild Herbaceous Plants, Allium victorialis var. platyphyllum, Ligularia stenocephala, and Panax ginseng in the Understory of Larix kaempferi Plantation

( Jae Mo Song ),( Jae-seon Yi ),( Eun Ju Cheong ) 한국산림과학회(구 한국임학회) 2019 한국산림과학회지 Vol.108 No.4

Two edible plants, Allium victorialis var. platyphyllum and Ligularia stenocephala, and one medicinal plant, Panax ginseng, were cultivated in the understory of an artificial Larix kaempferi plantation for ten years. Growth characteristics (number of leaves and flower stalks per plant, and leaf length and width), survival rate, and yield (fresh weight of plants) per unit area (1 m2) were investigated one year after planting, and six and ten years following cultivation. P. ginseng and L. stenocephala survived at a high percentage for two years after planting. Results showed that P. ginseng had longer and thicker roots when aged; however, a large number of plants died and the yield was low. In contrast, almost half of A. victorialis var. platyphyllum died within two years of planting. The surviving plants grew well for ten years and the yield was increased. The leaf length and width of L. stenocephala increased; however, the survival rate and the number of leaves per plant decreased as the period of cultivation was extended. In contrast, A. victorialis var. platyphyllum survived at a lower rate (50%) than the two other crops (98% for L. stenocephala and 83% for P. ginseng) during the early cultivation period, with little change in the survival rate over an extended time; however, the yields increased. This species showed an increase in the number of flower stalks and leaves, and as a result, the larger leaves increased the yield. We evaluated the understory cultivation of three crops in a L. kaempferi plantation under natural conditions, with no irrigation or fertilization, and Allium victorialis var. platyphyllum showed the greatest growth potential among the three tested crops

The Community Structure of Benthic Macroinvertebrates of the Small Mountain Wetland in Gangwon Natural Environment Research Park

( Jae Mo Song ),( Gi Yeoung Lee ),( Jung Ho Park ),( Jae Seon Yi ) 한국임학회 2011 산림과학 공동학술대회 논문집 Vol.2011 No.-

Underestimation of endoscopic size in large gastric epithelial neoplasms

Jae Sun Song,Byung Sun Kim,Min A Yang,Young Jae Lee,Gum Mo Jung,Ji Woong Kim,Jin Woong Cho 대한소화기내시경학회 2022 Clinical Endoscopy Vol.55 No.6

Background/Aims: Endoscopic submucosal dissection (ESD) is an effective method for resecting gastric adenomas and adenocarcino-mas. A significant discrepancy was observed between endoscopic and pathological sizes in samples obtained from patients undergoingESD. This study elucidates the factors affecting size discrepancy after formalin fixation. Methods: The records of 64 patients with 69 lesions were analyzed, including 50 adenomas and 19 adenocarcinomas. Data on location,gross shape, histology, and size after fixation in formalin were collected. Results: The mean size of the resected specimen appeared to decrease after formalin fixation (37.5 mm prefixation vs. 35.8 mm post-fixation, p<0.05). The mean long axis diameter of the lesions was 20.3±7.9 mm prefixation and 13.4±7.9 mm postfixation. Size differ-ences in lesions smaller than 20 mm were significantly greater than those in lesions larger than 20 mm (7.6±5.6 mm vs. 2.5±5.8 mm,p<0.01). In multivariate analysis, a tumor size of ≥20 mm was found to be an independent factor affecting size postformalin fixation(p<0.05). Conclusions: The endoscopic size of lesions before ESD may be underestimated in tumors larger than 20 mm in size. Therefore, in-creased attention must be paid during ESD to avoid instances of incomplete resection.