Global Absolute Quantitation of Proteins in Human Whole Saliva by nLC-QIMS-TOF Employing MS^E

Cho, Ha Ra,Jin, Sung Giu,Park, Jun Seo,Kim, Han Sol,Choi, Yong Seok Korean Society for Mass Spectrometry 2017 Mass spectrometry letters Vol.8 No.4

While saliva can be considered as good biological fluid for monitoring biomarkers due to many advantages including its communication with blood and the non-invasive nature during its sampling, its applications to that purpose is still limited. As a part of efforts to expand the applications of saliva to the protein biomarker research, we carried out global absolute quantitation of proteins in human whole saliva (WS) by bottom-up proteomics techniques mainly based on nLC-Q-IMS-TOF employing $MS^E$. From the analyses of a pooled WS sample collected from 22 healthy Korean volunteers, 93 proteins ranging from $5.89{\times}10^1ng/mL$ (immunoglobulin heavy chain) to $1.59{\times}10^4ng/mL$ (${\alpha}-amylase$ 1) were confirmed. For the validation of the present results, human serum albumin in the same sample was quantitated by ELISA and its result was compared with that from the nLC-Q-IMS-TOF study. As a result, there was no significant difference between two results from individual approaches ($1.18{\times}10^4{\pm}0.03{\times}10^4 ng/mL$ from nLC-Q-IMS-TOF experiments vs. $1.23{\times}10^4{\pm}0.07{\times}10^4ng/mL$ from ELISA experiments, n=3, p=0.309). To our knowledge, this is the first global absolute quantitation of proteins in human whole saliva and information from the present study can be widely used as the first level reference for the discovery of new protein biomarkers from human whole saliva as well as for quantitative applications of human whole saliva proteins.

Quality Characteristics of Paste (Anggeum) Added with Peach Puree by Storage Period

Ha-Na Ra,Ga-Yeong Park,Kyung-Mi Kim,Yong-Sik Cho,Ha-Yun Kim 한국식품영양과학회 2018 한국식품영양과학회 학술대회발표집 Vol.2018 No.10

Global Absolute Quantitation of Proteins in Human Whole Saliva by nLC-QIMS- TOF Employing MS^E

( Ha Ra Cho ),( Sung Giu Jin ),( Jun Seo Park ),( Han Sol Kim ),( Yong Seok Choi ) 한국질량분석학회 2017 Mass spectrometry letters Vol.8 No.4

While saliva can be considered as good biological fluid for monitoring biomarkers due to many advantages including its communication with blood and the non-invasive nature during its sampling, its applications to that purpose is still limited. As a part of efforts to expand the applications of saliva to the protein biomarker research, we carried out global absolute quantitation of proteins in human whole saliva (WS) by bottom-up proteomics techniques mainly based on nLC-Q-IMS-TOF employing MS^E. From the analyses of a pooled WS sample collected from 22 healthy Korean volunteers, 93 proteins ranging from 5.89×10¹ ng/mL (immunoglobulin heavy chain) to 1.59×10⁴ ng/mL (α-amylase 1) were confirmed. For the validation of the present results, human serum albumin in the same sample was quantitated by ELISA and its result was compared with that from the nLC-Q-IMS-TOF study. As a result, there was no significant difference between two results from individual approaches (1.18×10⁴ ± 0.03×10⁴ ng/mL from nLC-Q-IMS-TOF experiments vs. 1.23×10⁴ ± 0.07×10⁴ ng/mL from ELISA experiments, n=3, p=0.309). To our knowledge, this is the first global absolute quantitation of proteins in human whole saliva and information from the present study can be widely used as the first level reference for the discovery of new protein biomarkers from human whole saliva as well as for quantitative applications of human whole saliva proteins.

Determination of Emamectin Benzoate in Eel, Halibut, and Shrimp Using QuEChERS-EDTA and LC-MRM

Cho, Ha Ra,Kim, Dong Yoon,Lee, Hyo Chun,Han, Sang Beom,Shin, Ho-Chul,Choi, Yong Seok Korean Society for Mass Spectrometry 2018 Mass spectrometry letters Vol.9 No.4

As a part of efforts to establish the positive list system (PLS) in South Korea, a method to determine residual emamectin benzoate (EB) in various aquatic products using QuEChERS-EDTA and LC-MRM was developed. The developed method was validated in the aspects of specificity, linearity (correlation coefficient of at least 0.996), sensitivity (the limit of detection and the lower limit of quantitation ${\leq}5ng/g$), recovery (the recovery range of 87.4 and 96.2), and precision (the relative standard deviation of recovery between 0.9 and 13.5). Additionally, the validated method was successfully applied for monitoring EB contamination in eel, halibut, and shrimp collected from local food markets. To our knowledge, the present method is the first one to determine residual EB in various aquatic products at the level satisfying the PLS and could contribute to the establishment of the PLS in South Korea.

Original Articles : Case report : Canine distemper virus infection in a wild Korean raccoon dog

( A Ra Cho ),( Yoon Seok Roh ),( Hae Beom Lee ),( Ho Seong Cho ),( Chae Woong Lim ),( Seog Jin Kang ),( Ha Young Kim ),( Jong Wan Kim ),( Bum Seok Kim ) 한국예방수의학회(구 한국수의공중보건학회) 2015 예방수의학회지 Vol.39 No.1

Abstract: A female wild raccoon dog was referred with a history of generalized seizure. Mild leukocytosis was noted onlaboratory tests. Gross lesions included nasal hemorrhage, hemothorax, and hemorrhage in the urinary bladder withhematuria. Microscopically, interstitial and purulent bacterial pneumonia was observed in the lungs. In the cerebellum,characteristic eosinophilic intracytoplasmic and intranuclear inclusion bodies were found in Purkinje cells, and severedemyelination was observed in the cerebellar white matter. Canine distemper virus (CDV) infection was suspected andconfirmed after detection of CDV nucleoprotein RNA in the cerebrum and the lungs by nested reverse transcriptionpolymerase chain reaction (RT-PCR) and reverse transcription loop-mediated isothermal amplification (RT-LAMP). Based onthe histopathological and molecular diagnostic findings, it was concluded that the raccoon dog was infected with CDV.

Quality Characteristics of Tteokbokki Tteok Varying Packing Unit by Heat Treatment

Sa Ra Park,Ok lan Sung,Ha Na Ra,Kyung Mi Kim,Yong Sik Cho,Ha Yun Kim 한국식품영양과학회 2018 한국식품영양과학회 학술대회발표집 Vol.2018 No.10

AI를 활용한 손가락 인식 및 가상 터치 서비스

조아라 ( A-ra Cho ),유승배 ( Seung-bae Yoo ),윤병훈 ( Byeong-hun Yun ),조형주 ( Hyung-ju Cho ),하광림 ( Gwang-rim Ha ) 한국정보처리학회 2023 한국정보처리학회 학술대회논문집 Vol.30 No.2

코로나-19로 인해 비접촉 서비스의 중요성이 더욱 대두되고 있다. 키보드나 마우스와 같은 기존 입력 장치를 대체하기 위해 사람들은 디지털 기기에서 손을 사용하여 자연스럽고 간단한 입력을 할 수 있게 되었다. 본 논문에서는 미디어파이프(MediaPipe)와 LSTM(Long Short-Term Memory) 딥러닝을 활용하여 손 제스처를 학습하고 비접촉 입력 장치로 구현하는 방법을 제시한다. 이러한 기술은 가상현실(VR; Virtual Reality), 증강현실(AR; Augmented Reality), 메타버스, 키오스크 등에서 활용 가능성이 크다.

유도분만의 성공 예측인자로서 자궁경부 길이, 부피, 히스토그램의 평가

김윤하 ( Yoon Ha Kim ),김종운 ( Jong Woon Kim ),김철홍 ( Chul Hong Kim ),조문경 ( Moon Kyoung Cho ),조혜연 ( Hye Yon Cho ),조아라 ( Ah Ra Cho ),강우대 ( Woo Dae Kang ),김석모 ( Seok Mo Kim ),송태복 ( Tae Bok Song ) 대한산부인과학회 2010 Obstetrics & Gynecology Science Vol.53 No.5

목적: 유도분만시 Bishop 점수와 초음파를 이용하여 측정한 자궁경부 길이, 부피, 경부조직의 히스토그램 값으로 유도분만의 성공 예측이 가능한지 알아보고자 하였다. 연구 방법: 임신 34주에서 41주 사이의 유도분만이 예정된 Bishop 점수가 6점 이하인 41명의 미산부를 대상으로 하였다. 프로스타글란딘 E2 (PGE2, Propess(R); Controlled Therapeutics Ltd) 페서리를 사용하였고, 자궁수축 유무에 따라 옥시토신을 추가 사용하여 유도분만을 시행하였다. ACCUVIX XQ (Medison) 초음파의 3차원 경질 탐촉자를 이용하여 자궁경부의 길이, 부피, 경부조직의 히스토그램값을 측정하였고 Bishop 점수는 내진을 통해 측정하였다. 유도분만의 성공은 PGE2 페서리 제거 후12시간 이내에 4 cm 이상의 자궁경부 개대를 보이는 활성기에 도달한 경우로 정의하였다. 결과: 유도분만 성공률은 73.2% (30/41)였다. 다중 로지스틱 회귀분석에서 전방부 히스토그램 값이 유일하게 유도분만 성공 예측과 관계가 있었다. Receiver operating characteristic (ROC) curve 분석을 통해 유도분만 성공을 예측할 수 있는 전방부 히스토그램 값은 69.4였다. 결론: 질식 초음파를 이용한 전방부 히스토그램 측정값은 유도분만의 성공과 연관이 있다. 그러나 Bishop 점수와 자궁경부의 길이, 부피와 후방부 히스토그램 값은 성공여부를 예측할 수 없다. Objective: The aim of this study was to evaluate whether the Bishop score, length, volume and gray-scale histogram of the cervix has a predictive value of assessing the rate of success in trial of induction. Methods: Forty-one nulliparous patients with its Bishop score six or less were enrolled for this prospective study. All were on prostaglandin E2 (PGE2, Propess(R); Controlled Therapeutics Ltd) pessary. Three-dimensional transvaginal ultrasound scans of the cervix were performed on the ACCUVIX XQ (Medison) to measure length, volume, and gray-scale histogram. Bishop score was determined by digital examination. The successful induction was defined as the ability to achieve the active phase of labor corresponding to a cervical dilatation of ≥4 cm within 12 hours of removing the PGE2 pessary. The receiver operating characteristics (ROC) curves were also used to estimate an optimal cutoff point for the Bishop score, length, volume, and gray-scale histogram of the cervix. Logistic regression analysis was used for statistical analyses. Results: The overall successful rate of labor induction was 73.2% (30/41). Multiple logistic regression analyses demonstrated that the value of anterior lip histogram was significantly associated with the successful labor induction. ROC curve for anterior lip histogram value in predicting success of induction indicated a significant relationship with successful induction. The best cutoff value was 69.4. Conclusion: The value of anterior lip histogram associates significantly with the prediction of successful induction in nulliparous women. But, Bishop score and other sonographic measurement of cervical length, volume, and posterior lip histogram have no predictive values for successful induction.

S-287 Serum Fibroblast Growth Factor 21 is a Predictable Marker of Coronary Artery Disease i n Type 2 DM

( Ha-young Choi ),( Hyeok-gyu Lee ),( A-ra Cho ),( Sang-ho Park ),( Seung-woon Rha2 ) 대한내과학회 2016 대한내과학회 추계학술대회 Vol.2016 No.1

Introduction: Obesity, metabolic syndrome and diabetes mellitus are risk factor of coronary artery disease (CAD). Fibroblast growth factor 21 (FGF21) is secreted primarily from liver that exerts potent anti-diabetic and lipid lowering effects in animal models of obesity and type 2 diabetes mellitus. In recent studies showed FGF21 has similar effects in human subjects and also related with obesity and metabolic syndrome. However, the relationship between serum FGF21 level and CAD is not yet unknown. Objective: This study aimed to investigate the relationship among serum FGF21 levels, various cardiometabolic parameters and CAD in type 2 diabetic patients. Methods: In a total of 168 Korean adults with diabetes mellitus, in whom coronary angiogram were performed and classified into CAD group (n=99) and non-CAD group (n=69). Significant coronary artery stenosis was defiened as a coronary angiography finding of more than 50% stenosis in 1 ≥ coronary arteries. Fasting serum FGF21 level were measured using ELISA. Results: Serum mean FGF21 level in type 2 diabetes mellitus with CAD showed lower than in type 2 diabetes mellitus without CAD (1.10±0.22 pg/mL vs. 1.27±0.16 pg/mL, p<0.05). Conclusions: The Result suggest that fasting serum FGF21 levels might be a predictive marker of coronary artery diseases in Korean patients with type 2 diabetes mellitus

Quantitative assessment of steroid amount in the tissue after epidural steroid injection: a new rabbit model

Cho, Jungheum,Lee, Joon Woo,Lee, Eugene,Kang, Yusuhn,Cho, Ha Ra,Kim, Dong Yoon,Ho, Myoung Jin,Kang, Myung Joo,Choi, Yong Seok The Korean Pain Society 2019 The Korean Journal of Pain Vol.32 No.4

Background: To develop a rabbit epidural steroid injection (ESI) model for analyzing steroid retention in the tissue, and to assess the difference in steroid retention in the model according to the location and time elapsed after ESI. Methods: Fluoroscopy-guided ESI was performed using the interlaminar approach between the lowest two lumbar segments in 13 female New Zealand white rabbits. Four rabbits were allocated to each of three different groups according to the time of sacrifice: 3, 7, and 15 days post-ESI; the remaining rabbit was sacrificed immediately post-ESI to obtain baseline data. After sacrifice, two segments were harvested: the lowest two lumbar vertebrae and another two lumbar vertebrae immediately above these. The residual steroid amount (RSA) and residual steroid concentration (RSC) in the collected spinal columns were analyzed. A linear mixed model was used to compare RSAs and RSCs between the injected and adjacent segments, and among the number of days until sacrifice; P < 0.05 was considered statistically significant. Results: Both RSA and RSC of the injected segment were significantly higher than those of the adjacent segment (P < 0.001, both). The RSA and RSC significantly decreased over time (P = 0.009 and P = 0.016, respectively). Conclusions: The developed rabbit ESI model verified that significantly more steroid was retained at the injected segment than at the adjacent segment and the residual steroid decreased over time. This model could be useful not only for comparing current steroid medications, but also for developing new, better steroid formulations.