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류경원,장영남,배인국,채수천,최상훈 한국광물학회 2004 광물과 암석 (J.Miner.Soc.Korea) Vol.17 No.3
딕카이트[Al₂Si₂O_(5).(OH)₄]를 출발물질로 사용하여 이팔면체형 스멕타이트를 수열합성하였다. 시료를 활성화시키기 위해 Na₂O 성분을 첨가하고 800℃에서 4시간 열처리하였다. 합성실험을 Na-0.7 바이델라이트의 화학반응식에 의한 화학양론적 조성에 따라 SiO₂성분을 첨가하였으며 300℃, 70 ㎏f/㎠ 이하의 조건에서 온도, 압력, 시간 등을 변화시키면서 실험을 수행하였다. 합성실험을 위해 약 1리터 용량의 밀폐형 강철재 압력용기를 사용하였다. 스멕타이트를 합성할 수 있는 최적 조건은 반응온도 290℃, 반응시간 48시간, pH 10 및 60 ㎏f/㎠ 의 압력조건인 것으로 확인되었다. 온도, 압력조건 외에 원료물질의 활성화, 반응시간, 반응용액의 초기 pH 등은 결정도에 영향을 미치는 주요 요인으로 작용하였다. 합성결과물에 대한 X-선 회절분석, 에틸렌글리콜 처리, 'Greene-Kelly' 측정법 등의 실험결과, 합성된 스멕타이트는 Na-바이델라이트임이 확인되었다. A hydrothermal process was used to synthesize dioctahedral smectite from dickite [Al₂Si₂O_(5).(OH)₄]. Dickite was previously activated by heating at 800℃ for 4 hours with Na₂CO₃. After the heat-treatment, Sift was added for stoichiometrv. The autoclaving was carried out in closed stainless steel vessel (about 1 liter) at the condition of various temperature, pressure, time etc. High quality smectite could be obtaind by heating at 290℃ under the pressure of 60 ㎏f/㎠ for 48 hours. This experiment reveals that pH of the solution was an important factor and should be maintained at 10 to 11 for the formation of dioctahedral smectite. Be synthesized smectite was identified as Na-beidellite by the treatment of ethylene glycol and Greene-Kelly test.
개 대뇌겉질에서 Platelet-Derived Growth Factor α-Receptor의 출생 후 발달에 관한 면역조직화학적 연구
윤영,안병수,김인정,양경철,박선홍,김기훈,박도영,김장만,문정석,장인엽,조하영 조선대학교 부설 의학연구소 2002 The Medical Journal of Chosun University Vol.27 No.1
Background and Objectives : The localization of platelet-derived growth factor-α receptor (PDGF-α R) was commonly restricted to oligodendrocyte progenitors during late embryonic and postnatal development. However, several studies recently demonstrated that mature neurons could also synthesize PDGF-α, Materials and Methods : In the present study, to analyze the distributional pattern of PDGF-αR during postnatal development of the canine cerebral cortex, we used immunohistochemistry on sections of canine brain tissue. Results : We found that neurons of various regions of cerebral cortex exhibited the immunoreactivity to PDGF-αR as early as postnatal day 0, and slightly decreased after postnatal day 14. Whereas neuronal PDGF-αR were maintained at all ages, the oligodendroglia-like expression of PDGF-αR could not be confirmed. Conclusion : The localization of PDGF-αR in immature and mature neurons supports the several roles of PDGF during development, protection and survival of neurons.
생쥐 위장관의 Interstitial cells of Cajal(ICC)에 대한 면역조직화학적 연구
김영철,차경훈,신무경,임건한,김주영,안병수,김장만,양경철,박도영,오재욱,장인엽 조선대학교 2003 The Medical Journal of Chosun University Vol.28 No.1
Background and Objectives : Interstitial cells of Cajal(ICC) are the pacemakers in gastrointestinal tract that modulates gastrointestinal motiliey and these cells also transmit neural input from enteric nerves to smooth muscles. Recent work on tissues from patients with motility disorders that suggest that loss or defect in ICC could be related to pathophysiology in human and animal models. Immunolabelling of ICC in intestinal wall is recently developed by using specific marker, anti-c-kit antibody. Immunohistochemistry was done for ICC network in attempt to provide a morphological basis for the mechanism regulating gastrointestinal motility Methods : Cryosection was done, and whole-mount preparations of mouse stomach, gastrointestinal tract were immunolabelled using the anti-c-Kit. Immunolabelled ICC networks were observed under a confocal laser scanning microscopy. Results : According to three dimensional reconstruction study, we found that the c-Kit-positive celluar networks were widely distributed in the gastrointestinal muscle (1) circular muscle layer(IC-IM), (2) myenteric plexus(IC-MY), (3) deep muscular plexus(IC-DMP) in ileum, (4) submucosal plexus(IC-SMP) and longitudinal muscle layer(IC-LM) in colon. Conclusion : The characteristic profiles of ICC celluar networks provide a morphological basis upon the mechanism regulating gastrointestinal motility. Additional studies for the enteric nerves-ICC interaction are need to evaluate the detailed roles of Icc in gastrointestinal tract.
( Seung Soo Yoo ),( Hyo-gyoung Kang ),( Jin Eun Choi ),( Mi Jeong Hong ),( Sook Kyung Do ),( Jang Hyuck Lee ),( Won Kee Lee ),( Shin Yup Lee ),( Jaehee Lee ),( Seung Ick Cha ),( Chang Ho Kim ),( Eung 대한내과학회 2020 The Korean Journal of Internal Medicine Vol.35 No.4
Background/Aims: Genome wide and candidate gene association studies have identified polymorphisms associated with the risk of lung cancer in never-smokers. This study was conducted to evaluate the association between 11 polymorphisms identified in female never smokers and the lung cancer risk in male smokers. Methods: This study included 714 lung cancer patients and 626 healthy controls. The polymorphisms were genotyped using SEQUENOM MassARRAY iPLEX assay or Taq-Man assay. Results: Two polymorphisms were associated with the risk of lung cancer in male smokers, as in female never smokers. Male smokers carrying the rs4975616 variant allele had a significantly decreased risk of lung cancer (in a codominant model: odds ratio, 0.77; 95% confidence interval, 0.61 to 0.96; p = 0.02). The rs9387478 polymorphism also reduced lung cancer risk in male smokers (in a codominant model: odds ratio, 0.85; 95% confidence interval, 0.73 to 0.997; p = 0.046). In a stratified analysis, the association between these polymorphisms and the risk of lung cancer was predominant in lighter smokers and for cases of adenocarcinoma. Conclusions: These results suggest that a subset of polymorphisms known to be associated with the risk of lung cancer in female never smokers is also associated with the risk of lung cancer in male smokers.
( Seung Soo Yoo ),( Jae Yong Park ),( Jang Hyuck Lee ),( Mi Jeong Hong ),( Jin Eun Choi ),( Hyo-gyoung Kang ),( Sook Kyung Do ),( Won Kee Lee ),( Sun Ha Choi ),( Yong Hoon Lee ),( Hyewon Seo ),( Jaehe 대한결핵 및 호흡기학회 2021 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.129 No.-
Background Bromodomain and extraterminal domain (BET) proteins are epigenetic readers that regulate gene expression. We investigated whether variants in BET genes are associated with survival outcomes of lung cancer. Methods The associations between 77 variants in BET family genes and survival outcomes were analyzed in 773 non-small cell lung cancer patients who underwent surgery (349 and 424 patients in the discovery and validation cohorts, respectively). Results Six variants were associated with overall survival (P < 0.05) in the discovery cohort and one variant (rs2506711C >T) was replicated in the validation cohort. BRD3 rs2506711C>T is located in the repressed area and has a strong linkage disequilibrium (D' and r2 = 0.98) with rs2427964C>T in the promoter region. BRD3 rs2427964C>T was associated with worse overall survival in the discovery cohort, validation cohort, and combined analysis (under a recessive model: adjusted hazard ratio [aHR] = 1.70, 95% confidence intervals [CIs] = 1.11-2.61, P = 0.02; aHR = 1.97, 95% CIs = 1.18-3.28, P = 0.01; and aHR = 1.92, 95% CIs = 1.39-2.65, P = 8 × 10-5, respectively). In the luciferase assay, promoter activity was significantly higher in the BRD3 rs2427964 T allele than in the BRD3 rs2427964 C allele, which selectively bound with SIN3A, a transcriptional repressor. Knockdown of BRD3 with BRD3-specific siRNA decreased the proliferation and migration of lung cancer cells. BRD3 silencing also induced G2/M cell cycle arrest and increased apoptosis rate. These Results suggests that BRD3 rs2427964C>T increases BRD3 expression through increased promoter activity, which has been associated with poor prognosis for lung cancer. Conclusions BRD3 rs2427964C>T was associated with a poor survival outcome in non-small cell lung cancer patients.