黃帝內經에 나타난 寒의 槪念, 病理 및 病證에 관한 文獻的 考察

金聖勳,鄭燦吉,李光浩 대한동의병리학회 1994 동의생리병리학회지 Vol.9 No.1

연구배경 : 한의학에서 병인증에 중요한 위치를 점하고 있는 육기 변화의 실상중 한사의 개념 병기 병증 등을 내경을 중심으로 살펴보아 정리하여 정확한 동의 병리학의 이론 정립에 기여하고자 실시하였다. 방법 : 황제내경에서 한자가 포함된 문구를 찾고 한사의 개념 병기 병증 등을 이에 관한 역대주가의 설을 종합적으로 정리하고 고찰하였다. 결과 : 寒은 冬季에 閉臧를 主管하는 氣로서 自然界의 四時變化와 方位 및 地 方에 根據하고 있으며, 寒의 性質에 關해서는 <<痺論>> <<調經論>> 및 <<刺志論>> 등에서는 寒이 陰邪로서 陽氣虛와 關聯됨을, <<陰陽應象大 論>> <<離合眞邪論>> <<經絡論>> <<八正神明論>>에선 寒의 凝泣性質을, <<擧痛論>>에서는 寒의 收引性과 통증과의 관계를 示唆하고 있었으며, 寒症은 外部의 寒邪가 侵入하여 發生되는 外寒症과 內部의 陽氣不足으로 인하여 發生하는 內寒症으로 나누었고, 한에 대한 진단에 있어서 각종 體液 排泄物의 狀態로서 寒熱을 判別하는 根據가 言及되었다. 결론 : 한의 개념, 병리 및 병증에 대해 황제내경 이후 역대의가설을 종합적으로 비교 검토하는 연구가 필요하다고 사료된다.

배합사료의 공급체계에 대한 소비자의 의식조사

김종호,정찬길,이종인 한국식품유통학회 2005 食品流通硏究 Vol.22 No.1

The survey was focused on understanding of livestock farmers for feed supply system. Questionnaire was given to 181 livestock farmers who raise Korean cattle and milking cow, swine, layer, broiler, and other livestock. The questionnaire was given by livestock that farmers were raising animals and distribution channels that farmers were purchasing feed. Questions were given as follow. Task of distribution channels, feed price, preferred feed seller, price-cutting, necessity of firms that sell feeds manufactured by various firms, how to cut distribution cost, sliding scale system for feed price, etc. A few rationalization plans for feed supply system were suggested from the results of the questionnaire.

PCR 기법을 이용한 한국재래산양 β-casein 유전자의 특성

김지애,류승희,유성란,이준헌,서길웅,김선균,상병찬 충남대학교 농업과학연구소 2002 농업과학연구 Vol.29 No.2

본 연구는 한국 재래 산양 112두와 유산양인 Saanen종 7두의 혈액으로부터 genomic DNA를 추출하고, PCR-RFLP 방법에 의해 β-casein 유전자의 특성을 분석하여 한국재래산양의 효율적인 유전자원의 보전 및 개량을 위한 기초 자료로 제공하고자 실시하였다. 한국재래산양의 genomic DNA로부터 PCR기법을 이용하여 β-casein의 유전자좌를 증폭한 결과 각각 481bp 크기의 단편이 양호하게 증폭되었음을 확인하였다. β-casein 유전자좌의 증폭산물에 대한 Bal Ⅰ의 제한효소를 처리한 결과, β-casein AB형은 481bp, 284bp 및 197bp의 단편을, 그리고 BB형은 284BP와 197BP의 단편을 한국재래산양과 유산양인 Saanen 종에서 확인 할 수 있었다, 유전자형 빈도에 있어서는 한국재래산양에서 β-casein AB 및 BB의 빈도는 각각 6.25 및 93.75%이었고, 유산양인 Saanen 종은 각각 57.14 및 42.86%이었다. 유전자빈도에 있어서는 한국재래산양의 β-casein A 및 B의 빈도가 각각 0.031 및 0.969이었고, Saanen 종에서는 각각 0.286 및 0.714의 빈도를 보였다 한국재래산양의 β-casein 유전자의 염기서열과 이미 보고되어 있는 goat의 염기서열(GeneBank accession Number M90556)간에는 총 11개의 염기서열에 차이를 나타내어 97.71%의 상동성을 보였다, 따라서 한국재래산양의 β-casein 유전자의 다형성과 염기서열 분석에 의한 분자유전학적 특성의 규명은 한국재래산양의 유전자원의 보전 및 개량을 위한 기초 및 응용 자료로 이용될 수 있을 것으로 생각된다. This study was performed to provide the basic data for preservation and improvement of genetic resources according to finding genetic construction obtained from analysis of genetic characteristics of β-casein gene in Korean Native goat and Saanen using the PCR-RFLP. This study confirmed the amplified products of 481bp fragments obtained from the amplification of β-casein loci by PCR. The β-casein AB genotype showed 481, 284 and 197bp, and β-casein BB genotype showed 284 and 197bp fragments in Korean Native goat and Saanen. The frequencies of β-casein genotype in Korean Native goat were 6.25 and 93.75% for AA and AB and the frequencies of β-casein genotype in Saanen were 57.14 and 42.86% for AA and AB types. The frequencies of β-casein A and B alleles were 0.031 and 0.969 in Korean Native goat and the frequencies of β-casein A and B alleles are 0.286 and 0.714 in Saanen, respectively. The nucleotide sequence of β-casein gene of Korean Native goat was 97.71% higher homology with 11 nucleotide sequences difference of that of goat reported in GeneBank (M90556). Therefore, this study of molecular genetic characteristics by the analysis of genetic polymorphism and sequencing for β-casein gene should be used as basic and applying data for preservation and improvement of genetic resources in Korean Native goat breeding.

21세기 노인여가정책의 발전방향에 관한 연구

김흥태,박찬길,유신환 강원대학교 체육과학연구소 2001 江原大學校附設體育科學硏究所論文集 Vol.- No.24

The purpose of this study is to propose a study on the direction of development of the leisure policy in the aging society in the 21st century and ultimately try to find how to activate the old People's exercises. The method to develop the leisure policy for the old people's as follows: First there must be creative, active and constructive form of leisure and we must help the old to enjoy a life of leisure. Secondly, it is necessary to expand and supplement recreation facilities for the aged in the aging society, Thirdly, it is necessary to enlarge the public facilities for the old people's physical training and find a plan to use them properly. Fourthly, it is necessary to fry to find the method to use the facilities effectively for a life of leisure. Fifthly, there must be a variety of educational program to activate a leisure activity for the aged and it is necessary to strength then the old people's education through all cultures. Sixthly, it is necessary to instruct and use professional leaders to carry out such leisure activities for the old. Seventhly, it is necessary to develop and spread the sports programs proper to the old and then find a plan to utilize them wisely, Last, including the leisure policy for the aged, it is necessary to set up and intensify an organization exclusively responsible for the old people's physical training.

Pseudomonas sp. 로부터 생산되는 phytase에 관한 연구

김대영,양시용,권문남,김찬길 建國大學校 自然科學硏究所 2002 建國自然科學硏究誌 Vol.13 No.2

Phytase (myo-inositol hexakisphosphate phosphohyrolase) is found naturally in plants and microorganisms. It catalyses pydrolysis of phytate to release inorganic phosphate, which would decrease the addition of phosphorus to feedstuffs for monogastric animals and thus reduce environmental pollution. this study was carried out for examination of characteristic of phytase from Pseudomonas sp. The phytase from Pseudomonas sp. was partially purified by DEAE-Sepharose chromatography and its molecular weight was estimated to 45kDa by SDS-PAGE Partially purified phytase was stable at pH values ranging from 5.0~7.0, and it was stable to 40℃.

혼합형 자가면역성용혈성빈혈과 동반된 Evans 증후군 1례

선길홍,윤찬영,박상곤,박경희,우정주,한경택,김진화,김영훈,정춘해,박치영 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.2

Evans syndrome is defined as a simultaneous or sequential occurrence of autoimmune hemolytic anemia and autoimmune thrombocytopenia. Autoimmune hemolytic anemia (AIHA) is usually induced by IgG warm autoantibody or cold-active IgM antibodies reacting specifically with antigens associated with a patient's RBC. AIHA is a fairly uncommon disorder, with estimates of the incidence at 1 to 3 cases per 100,000 per year. Mixed-type AIHA is a relatively uncommon form of AIHA, with studies noting the incidence of 7-8% among cases of AIHA. We experienced a patient, 46-year-old woman who was diagnosed having a very rare clinical presentation of mixed warm and cold antibody mediated Evans syndrome. She was treated with corticosteroid therapy only and has been maintaining a complete response for 15 weeks. 저자들은 혼합형의 자가면역성 용혈성 빈혈과 자가면역성 혈소판 감소증이 동시에 발생한 혼합형 Evans 증후군으로 진단하고 스테로이드 요법 후 혈액학적으로 회복된 상태로 15주가 지난 현재 steroid 5 mg/일 까지 감량한 상태에서 추적 관찰 치료중인 46세 여자 환자를 경험하였기에 문헌 고찰과 함께 보고하는 바이다.

개구부가 있는 밀폐공간내 화재의 복합열전달 및 연소가스 분석에 관한 연구

박찬국,추병길,김철 (社團法人)韓國火災·消防學會 1997 한국화재소방학회논문지 Vol.11 No.1

The natural convection and combined heat transfer induced by fire in a rectangular enclosure is numerically studied. The model for this numerical analysis is partially opened right wall. The solution procedure includes the standard k-ε model for turbulnt flow and the discrete ordinates method (DOM) is used for the calculation of radiauve heat transfer equation. In numerical study, SIMPLE algorithm is applied for fluid flow analysis, and the investigations of combustor gas induced by fire is performed by FAST model of HAZARDI program. In this study, numerical simulation on the combined naturnal convection and radiation is car ried out in a partial enclosure fifed with absorbed emitted gray media, but is not considered scattering problem. The streamlines, isothermal lines, average radiation intensity and kinetic energy are compared the results of pure convection with those of the combined convection-radiation, the combined heat transfer. Comparing the results of pure convection with those of the combined convection-radiation, the combined heat transfer analysis shows the stronger circulation than those of the pure convection. Three different location of heat source are considered to observe the effect of heat source location on the heat transfer phenomena. As the results, the circulation and the heat transfer in the left region from heating block are mich more influenced than those in the right region. It is also founded that the radiation effect cannot be neglected in analyzing the building in fire. And as the results of combustion gas analysis from FAST model, it is found that O_2 concentration is decreased according to time. While CO and CO_2 concentration are rapidly increased in the beginning(about 100sec), but slowly decreased from that time on.

CAD를 이용한 양수장 및 배수장설비 설계의 표준화

권병철,권경우,김기길,명태식,박찬호,박귀용 한밭대학교 산업과학기술연구소 1995 논문집 Vol.3 No.-

This paper is to be illustrated automation in the design of Pumping Station and Draining Station. Program for design automation applying Auto LISP, DCL can be used to design and draw simultaneously. The study result are as follows. 1. Optimal design has been introduced and precise design has been possible using CAD. 2. Dialog design is possible with introduction the concept of design automation, and design cost can be reduced greatly.

혈액투석 환자에게서 발생한 재생불량성 빈혈 1예

정지용,윤나라,강대웅,오종찬,장재현,선길홍,박경희,정종훈,김현리 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.1

Aplastic anemia is a disease characterized by pancytopenia in the peripheral blood and hypocellular bone marrow with the fatty infiltration. The causes of the acquired aplastic anemia were usually related to drugs and immune-related diseases. A 34-years old man was admitted due to general weakness. He has been going hemodialysis for 2 years because of end stage renal disease (ESRD) due to chronic glomerulonephritis. At admission, laboratory tests showed hemoglobin 6.2 g/dL, hematocrit 18.0 %, WBC 5,710mm (neutrophil 71.8%, lymphocyte 19.1%, monocyte 5.9%), reticulocyte 1.9%, platelet 93,000/mm, Fe 12.8 ug/dL, TIBC 204 ug/dL, ferritin 941.47 ng /ml, haptoglobin 0.72 g/L, vitamin B12 508.17 pmol/L, folate 24 ng/mL, total protein 6.54 g/dL, albumin 3.76 g/dL, Alk. pohsphatase 79 IU/L, AST 30.0 IU/L, ALT 39.7 IU/L, total bilirubin 0.57 mg/dl, BUN 79.4 mg/dl and creatinine 10.18 mg/dl. Peripheral blood smear showed the normocytic normochromic anemia with anisocytosis. Anemia was diagnosed but it was irresponsive to the recombinant human erythropoietin (EPO) and blood transfusion. In the bone marrow aspiration smear were there the hypoplastic myeloid and erythroid precursors. The bone marrow biopsy section showed the hypoplasia of all components (10-20%) and the fatty infiltration. We have experienced one case of the idiopathic aplastic anemia in a patient going through hemodialysis and we just report it with documentary records. 저자들은 혈액 투석 중인 환자에서 약물, 면역계 이상등의 원인을 발견할 수 없었음에도 재생 불량성 빈혈이 발생한 1예를 경험하여 문헌 고찰과 함께 보고하는 바이다.

FCAnalyzer: A Functional Clustering Analysis Tool for Predicted Transcription Regulatory Elements and Gene Ontology Terms

Kim, Sang-Bae,Ryu, Gil-Mi,Kim, Young-Jin,Heo, Jee-Yeon,Park, Chan,Oh, Berm-Seok,Kim, Hyung-Lae,Kimm, Ku-Chan,Kim, Kyu-Won,Kim, Young-Youl Korea Genome Organization 2007 Genomics & informatics Vol.5 No.1

Numerous studies have reported that genes with similar expression patterns are co-regulated. From gene expression data, we have assumed that genes having similar expression pattern would share similar transcription factor binding sites (TFBSs). These function as the binding regions for transcription factors (TFs) and thereby regulate gene expression. In this context, various analysis tools have been developed. However, they have shortcomings in the combined analysis of expression patterns and significant TFBSs and in the functional analysis of target genes of significantly overrepresented putative regulators. In this study, we present a web-based A Functional Clustering Analysis Tool for Predicted Transcription Regulatory Elements and Gene Ontology Terms (FCAnalyzer). This system integrates microarray clustering data with similar expression patterns, and TFBS data in each cluster. FCAnalyzer is designed to perform two independent clustering procedures. The first process clusters gene expression profiles using the K-means clustering method, and the second process clusters predicted TFBSs in the upstream region of previously clustered genes using the hierarchical biclustering method for simultaneous grouping of genes and samples. This system offers retrieved information for predicted TFBSs in each cluster using $Match^{TM}$ in the TRANSFAC database. We used gene ontology term analysis for functional annotation of genes in the same cluster. We also provide the user with a combinatorial TFBS analysis of TFBS pairs. The enrichment of TFBS analysis and GO term analysis is statistically by the calculation of P values based on Fisher’s exact test, hypergeometric distribution and Bonferroni correction. FCAnalyzer is a web-based, user-friendly functional clustering analysis system that facilitates the transcriptional regulatory analysis of co-expressed genes. This system presents the analyses of clustered genes, significant TFBSs, significantly enriched TFBS combinations, their target genes and TFBS-TF pairs.