Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

음절말 자음 탈락에 의한 보상장음화 연구

이범진 대한언어학회 1997 언어학 Vol.5 No.2

Lee, Beom-jin. 1997. A Study of Compensatory Lengthening with Coda Consonant Deletion. Linguistics, 5-2, 309-325. The purpose of this paper is to analyse the compensatory vowel lengthening with coda consonant deletion within the framework of Moraic Phonology (Hayes 1989). Compensatory lengthening with consonant deletion occurs in the following cases: a. VηX > V X b. VNC > V C, Vg (C) > V (C) c. Vg > V Accounting for the consonant deletion and compensatory lengthening in (a), Hayes (1989:291-3) posits three moras in primitive Germanic. However, his analysis is untenable for cases such as (b). If we permit three moras in a syllable, there is a problem to analyze the so-called gemination. In this paper, I show that we can account for (b) by invoking the notion 'extrasyllabicity'.(Wonkwang University)

Effects of Caffeine and Exercise on Blood Levels of Dopamine and Serotonin in Male Humans

Jeong-Beom Lee,Young-Ki Min,Jeong-Ho Kim,Hee-Jin Lee3,Mi-Young Lee 한국식품영양과학회 2017 한국식품영양과학회 학술대회발표집 Vol.2016 No.10

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

소각잔사 중의 클로로벤젠과 클로로페놀의 함량 및 이성체 분포특성

이우근,심영숙,김진범 江原大學校 附設 環境硏究所 1998 環境硏究 Vol.15 No.-

Chlorobenzens(CBs) and chlorophenols(CPs) in fly asha discharged from several municipal soild waste incinerators (MSWI) were investigated in terms of total content and isomer distribution. The content of CBs and CPs was highest in the fly ash from S incinerator, which were 1040.7ngCB/g-fly ash and 1156.4ngCP/g-fly ash. According to the experimental results, while all samples contained a large amount of the higher chlorinated CBs the amount of lower chlorinated CPs varied with season It was found that the isomer distribution of CBs and CPs was almost constant regardless of season. The quantities of CBs and CPs depended closely on the chemical composition of MSW, while the isomer distribution of CBs and CPs was contant throughout all the year round.

왕우렁이(apple snails)의 생리ㆍ생태적 특성에 관한 연구

이상범,고문환,나영은,김진호 한국환경농학회 2002 한국환경농학회지 Vol.21 No.1

왕우렁이가 자연상태에 전파되어 서식되는 경로는 양식장의 배수시, 홍수로 인한 노지 양식장 붕괴 및 제초용 왕우렁이 입식에 의하여 이루어지고 있다. 왕우렁이의 생리적 특성을 보면 알 크기는 2.47 ㎜, 난괴크기는 1.8×4.3×0.94 ㎝, 알무게 12.78 ㎎, 산란수 157∼784개/1마리(평균 321개/마리) 유체크기 1.69×2.15㎜, 유체 무게 3.32㎎ 최저 산란 왕우렁이 크기 2.40×2.26 ㎝ 이상, 1개알 산란소요시간 22.4초 였다. 알의 색변화는 산란직후 우유빛의 연분홍에서 중기에는 선홍색, 부화직전에는 흐린 연보라색으로 변화하면서 부화된다. 왕우렁이 섭식 대상은 벼, 논잡초, 미나리, 토마토, (양)배추, 무, 호박, 콩잎 등 대부분의 농작물을 포함한 식물체 및 동족의 왕우렁이 등 수중동물이었다. 왕우렁이 월동지역은 장항, 장성 및 해남지역으로 양지녘의 식물체 줄기나 벼 그루터기에 산란된 알과 성체 상태로 저수지나 논의 물웅덩이에서 월동고, 5월 중순이후 수로의 벽이나 식물체 줄기에 산란하며 6월과 9월에 번식이 가장 왕성하였다. 서식지 수질특성은 변이 폭이 컸으나 pH가 7.07∼9.50 범위로 알칼리성에서 주로 번성하였다. 왕우렁이에 의한 벼 가해 양상중 바 발아초기에는 왕우렁이 크기에 관계없이 모두 벼싹을 가해하였고, 벼품종과 생육시기에 따라 차이가 있으며 어린묘 일수록 가해율이 높았으며, 이앙후 45일 벼(초장 약 73 ㎝)도 각고 3.3∼3.5 ㎝ 이상의 중형 왕우렁이는 가해하였다. This experiment was carried out to obtain some information about overwintering, physiological and ecological characteristics of apple snails. Another purpose of this experiment was to characterize an appetite for rice plants by apple snails and to elucidate their choice of fresh green ones (vegetables, some other crops, weeds in rice fields). The freshwater snails were found with higher population at sites abundant organic compounds such as plant debris and at regions with high temperature. They also prefer calcium-rich water. This is a naturally occurring process. Apple snails were exceptionally well-adapted to the south regions of Korea, especially Janghang, Jangseong and Haenam, even if the temperature of winter season is cold below 0℃. Apple snails were not very selective in their food choice and eat almost everything available in their environment. A snail have something called a radula in its mouth for grinding up its food. A apple snail also chews on fruits and young succulent plant barks. In case of reproduction, apple snails deposit about 157~784 (average of 321 eggs) milky white to pale orange colored eggs above the waterline. In approximately every 22.4 seconds a new egg appears. The total time needed to deposit a egg mass varies from 58 minutes~4 hours 13 minutes. Apple snails reproduct actively from May to June and from September to October. An appetite of apple snails for rice plants was the different depending on their size and growing stage for rice plants. Apple snails had a great appetite of rice plants as well as dropwort, tomato, cabbage, radish, aquatic plants etc. They preferred to eat young rice plants and drastically quit eating rice plants of over 40 cm in height. Thus considering the food preference of apple snail for various plants including rice, they were thought to be a potentially strong predator in fields, especially, at regions with warmer winter.

비대칭 군집분석법의 소개 및 사례분석

이광진,김범준 목원대학교 사회과학연구소 1998 社會科學硏究 Vol.- No.2

전통적인 군집분석 이론에서는 대칭인 거리행렬을 바탕으로 군집현상을 파악한다. 본 연구에서는 비대칭 상사성행렬이 주어진 경우 이를 거리행렬로 변환하지도 않으면서 비대칭 상사성행렬이 주어진 경우 이를 거리행렬로 변환하지도 않으면서 그리고 비대칭성도 그대로 유지하면서 개체들간의 군집 또는 결합현상을 분석할 수 있는 비대칭 군집분석(asymmetric cluster analysis)의 내용을 간략하게 정리하여 소개하면서, 두 개의 실 제자료를 통해 이의 유용성을 보이고자 함이 목적이다.

층수돌기 방선균증 : 1예 보고

이범하,이혜경,박성진,차장규,김희경 순천향대학교 의학연구소 2008 Journal of Soonchunhyang Medical Science Vol.14 No.2S

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소각장별 비산재 중의 중금속 용출 특성 및 존재형태

이우근,김준수,김진범 江原大學校 附設 環境硏究所 1998 環境硏究 Vol.15 No.-

본 연구는 도시폐기물 소각시설에서 발생되는 비산재 중에 함유된 중금속으 존재형태 및 용출 툭성을 알아보기 위해 수행되었다. 용출 실험은 KSLT, TCLP, MALT법을 이용하였고 중금속의 존재형태별 분포에는 다음과 같이 5가지 형태로 구분하였다.; exchangeable, carbonate, reducible, organic, residual fraction.용출 심험 결과에 의하면 pH 4로 일정하게 유지한MALT법에 의한 중금속 용출량이 가장 높 This study was carried out to estimate the fractional composition and leaching properties of heavy metals in fly ash generated from three municipal solid waste incinerators(MSWI). Three leaching tests are used in this work. These are Korea Standard Leaching Test(KSLT)), Toxicity Characteristic Leaching Procedure (TCLP), Maximum Availability Leaching Test(MALT) method. The fractional composition of heavy metals is classified into five fractions; exchangeable, carbonate, reducible, organic, residual fraction. According to the leaching tests, it seems that the highest amounts of heavy metals are leached by MALT method in which pH of 4.0 maintains constantly. From the results of the fractional composition of heavy metals, above 45% of Cd is leached by the change of pH or/and concentrations at I and J incinerators. And 91% of Cd is extracted under the reducible environment at B incinerator. But Cu may be leached under the strong acidic environment. The amount of Cu at B incinerator is less leached than that of I and J incinerators due to high residual fraction. About 35% of Ni in fly ashes at three incinerators exists in reducible fraction. It is probably leached under the reducible environment. Pb may be leached under the weak acidic environment at I and J incinerators, but in case of S incinerator leached under the strong acidic environment.

알긴산나트륨 및 첨가제를 함유한 서방성 매트릭스 정제

이범진,이태섭,신성이,허보욱,유승구 한국약제학회 1996 Journal of Pharmaceutical Investigation Vol.26 No.3

The matrix tablet containing sodium alginate and CaHPO₄ can release drugs in a controlled fashion from hydrogel with gelling and swelling due to their interaction as water penetrates the matrices of the tablet. The purpose of this study was to evaluate release characteristics of the matrix tablet varying the amount of sodium alginate, CaHPO₄ and other excipients such as chitosan, hydroxypropyl methylcellulose (HPMC) and Eudragit^ⓡ RS100 in the simulated gastric and intestinal fluid. The practically soluble ibuprofen was used as a model drug. The release profiles of matrix tablet in the gastric fluid as a function of sodium alginate/CaHPO₄ ratio was not pronounced because of low solubility of drug and stability of alginate matrices. However, release rate of drug from the matrix tablet in the intestinal fluid was largely changed when sodium alginate/CaHPO₄ ratio was increased, suggesting that the ratio of sodium alginate/CaHPO₄ was an important factor to control the gelling and swelling of the matrix tablet. The incorporation of other excipients into the matrix tablet also influenced the release rate of drug. The chitosan and HPMC decreased the release rate of drug. No release of drug was occurred when Eudragit^ⓡ RS100 was added into the tablet. The retarded release of matrix tablet when excipients were added resulted from the hindrance of swelling and gelling of the matrix tablet containing sodium alginate and CaHPO₄. The hardness and bulk density of the matrix tablet was not correlated with release rate of drug in the study. From these findings, the ratio of sodium alginate and CaHPO₄ in the matrix tablet in addition to incorporation of excipients could be very important to control the release rate of drug in dosage form design.