서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

Hepatic Cirrhosis Occurring in a Young Woodchuck(Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus(WHV)

Kyu-shik Jeong,정원일,Jae-yong Chung,Mi-young An,Chae-yong Jung,Gyoung-jae Lee,Jong-soo Kang,Byeong-cheol Kang,Young-heun Jee,Bruce H Williams,Young-oh Kwon,Da-hee Jeong 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

Cirrhosis Occurring in a Young Woodchuck (Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus (WHV)Da-hee Jeong, Won-il Jeong, Jae-yong Chung, Mi-young An, Chae-yong Jung, Gyoung-jae Lee1, Jong-soo Kang1, Byeong-cheol Kang2, Young-heun Jee3, Bruce H Williams4, Young-oh Kwon5 and Kyu-shik Jeong*College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea1Shinwon Scientific Co., LTD, Research Institute, Suwon, Korea2Clinical Research Institute, Seoul National University, Seoul 110-744, Korea3College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea

폐페인트 재활용공정 및 장치개발 연구

정재헌,한대상,조창제,이종무,장상목 동아대학교 공과대학부설 생산기술연구소 1998 生産技術硏究所硏究論文集 Vol.3 No.2

In this study, the process and equipment to recycle wastepaint were designed and development. The characteristic of this recycling process is to improve the recycling efficiency by dividing the previous recycling process into two processes, liquid-phase process and powder-phase process, with the hardness of wastepaint. As the result of this study, we confirm that this process may be helpful to recycle wastepaint.

側腦室內 Hexamethonium이 家兎賢臟機能에 미치는 影響

李載欣 충남대학교 의과대학 지역사회의학연구소 1982 충남의대잡지 Vol.9 No.1

1. The effects of hexamethonium, given into the lateral ventricle, on the renal function of the rabbits were examined, and its influence on the renal effects of intraventricular carbachol were also examined. 2. Intraventricular hexamethonium(300 ㎍/Kg) tended to slightly decrease urine flow rate, renal blood flow and glomerular filtration rate, but urinary excreted amounts of sodium, Cosm and free water reabsorption were decreased significantly from 20 or 40 min. after intraventricular hexamethonium. 3. Intraventricular carbachol (2㎍/ Kg) produced statistically significant diuresis, natriuresis and increased change of Cosm without significant changes of glomerular filtration rate and renal blood flow rate. 4. The effects of intraventricular carbachol were not influenced by the intraventricular hexamethonium pretreatment, but completly abolished by the intraventricular atropine pretreatment. From these above results, it is suggested that the centrally mediated diuresis of carbachol is not due to renal hemodynamic changes or ADH release, but related to other central muscarinic natriuresis.

재생간의 성장과정에 따른 ^3H-ouabain의 결합에 관한 연구

이재흔,허강민 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.2

To study the changes of Na, K-ATPase in the regenerating liver cell prepared the sinusoidal and canalicular membrane from normal and regenerating rat liver after partial hepatectomy, and Na, K-ATPase activity and ^3H-ouabain binding were measured. 1. In the control liver, Na, K-ATPase activity of sinusoidal membrane was 23.8μmole Pi/mg protein/hr and that of canalicular was 7.0 μmole Pi/mg protein/hr. But the numbers of ^3H-ouabain binding sites were almost same in both membranes. 2. In the regenerated liver for 3 days, the activity of Na, K-ATPase of sinusoidal membrane was decreased about 50% as compared to that of control liver. 5 days after partial hepatectomy, the activity of Na, K-ATPase was restored. 3. Ouabain concentration for the inhibition of Na, K-ATPase activity(IC_50) of sinusoidal membrane in the regenerated and control liver were 5.0 X 10 exp(-5)M and 3.8 X 10 exp(-5)M respectively. 4. In the ouabain binding experiment of sinusoidal membrane, there were little difference in Kd values between both group, but B_max in the regenerated liver was decreased about 50% compared to that of control liver. From the above results, it is suggested that the decreased Na, K-ATPase activity in the sinusoidal membrane during regeneration is due to the decreased numbers of ouabain binding sites, and Na, K-ATPase is distributed in canalicular membrane as well as sinusoidal membrane.

가토에서 일측 요관 부분 및 완전폐색시 신장과 요관의 조직학적 변화

김상현,김용웅,육승모,한동석,노안식,임재성,김홍식,나용길,설종구 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

This study was designed to clarify histologic changes in the experimental rabbit kidney and ureter after unilateral partial or complete ureteral obstruction. A total of 20 adult white rabbits were divided into 4 subgroups in the partial obstruction followed by 2 weeks, the partial obstruction followed by 4 weeks, the complete obstruction followed by 2 weeks, and the complete obstruction followed by 4 weeks. The normal control group was consisted of 5 adult white rabbits. The kidneys and ureters for light microscopy were fixed in 10% neutral formalin, embedded in paraffin and stained with hematoxylin and eosin. Abnormal histologic fingings showed in ipsilateral kidney and ureter of all subgroups(such as dilatation of uriniferous tubule, focal necrosis and desquamation of epithelium, interstitial fibrosis, glomerular congestion, widening of Bowman's space, dilatation of ureter, epithelial cell atrophy or loss, interstitial fibrosis and interstitial inflammation). These findings were most severe in the group of the complete-obstructed ureter for 4 weeks. No significant changes showed in contralateral ureter of all subgroups. The degree of obstruction was severe and period of obstruction was long, the histological changes in ipsilateral side were severe. Despite of changes in ipsilateral side no abnormal findings showed in contralateral side. We suggest that long term experimental investigation of histologic changes in ipsilateral side and compensatory changes in contralateral side have to be studied.

아드레날린 동작성 약물이 구강점막의 리도케인 Clearance에 미치는 영향

명재원,안명석,김중수,이종훈 대한구강생물학회 1992 International Journal of Oral Biology Vol.16 No.2

Some adrenergic drugs have been used as vasoconstrictors in local anesthetics for prolongation of acting duration and minimization of side effects. But, the exact role of vasoconstrictors on the clearance of local anesthetics in oral mucosa was not investgated. This experiment was carried out to clarify the influences of some adrenergic drugs on the clearance of lidocaine in oral mucosa. Sprague-Dawley rats were devided into control(no vasoconstrictor) group, epinephrine(1:100,000)-contained group, norepinephrine(1:30,000)-contained group and phenylephrine(1:2,500)-contained group. In urethane(1.2g/㎏) anesthetized rats, 300μl of 2% lidocaine with or without vasoconstrictor injected in the palatal mucosa. According to time schedule, they were sacrificed by exsanguination immediately, 30 and 60 minutes later after injection, respectively. The palatal mucosa was removed and homogenized by mechanical force and ultrasonicated in 0.1N HCl(500μl). The samples were centrifuged after akalization. Aliquat of the supernatant was injected into HPLC. Also, systemic effects of vasoconstrictors were evaluated with recording arterial blood pressures in common carotid artery. In control animals, lidocaine clearances reached 85.4% and 89.3% at 30 and 60 minutes after injection, respectively. At 30 minutes after injection, lidocaine clearances of vasoconstrictor contained group were different from control group significantly(p<0.01), and lidocaine clearance of phenylephrine contained group appeared higher than those of the other vasoconstrictor contained groups(p<0.05). But, at 60 minutes after injection, only epinephrine contained group had lower lidocaine clearance than control group(p<0.01). Intraperitoneal injection of the same dosage of palatal mucosa injecton of these vasoconstrictors did not elicit the change of blood pressure and heart rate.

일차배양된 설치류 호흡기 상피세포로부터의 점액소 분비에 대한 수종 약물의 영향

이충재,석정호,이재흔,허강민,박지선,배소현,노삼길,박상철 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

1. PKC activator인 PMA는 일차배양 HTSE세포로부터의 뮤신분비를 0.1μM 농도에서 30%, 1μM 농도에서 80% 가량 증가시켰다. 2. 식물 유래 성분으로, flavonoid의 일종인 TFR은 일치배양 HTSE 세포로부터의 뮤신분비를 10μM 농도에서 50%, 100μM 농도에서 80% 가량 증가시켰다. 3. 양이온성 폴리펩티드인 PLL 및 PLA는 일차 배양 HTSE 세포로부터의 뮤신분비를 0.01 - 10μM 농도에서 용량의존적으로 감소시켰다. 4. 결론적으로, 본 연구에서 얻어진 결과들은 새로운 거담제 및 점액용해제나 단백분해 효소제가 아닌 호흡기 류신의 생성/분비를 조절해 줄 수 있는 신개념의 약물을 개발함에 있어 극히 일부분이나마 단서를 제공하고 있다고 하루 수 있을 것이다. In the present study, we tried to investigate whether phorbol myristate acetate(PMA), trihydroxymethoxy-flavanone rutinoside(TFR) and cationic polypeptides significantly affect mucin release(secretion) from cultured hamster tracheal surface epithelial cells. Confluent primary hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled with 3H-glucosamine for 24 hr and chased for 30 min in the presence of each agent to assess the effect on 3H-mucin release. The results were as follows : (1) Both PMA and TFR significantly increased mucin release from cultured HTSE cells ; (2) Cationic polypeptides including po1y-L-lysine(PLL, mw 7,500) and poly-L-arginine(PLA, mw 10,800) significantly inhibited mucin release from cultured HTSE cells, in a dose-dependent manner. This finding suggests us that PMA and TFR be further studied for the possible use as mild expectorants and cationic polypeptides might function as a regulator for hyper-secretion of mucus, both by direct acting on airway mucin-secreting cells, during the treatment of chronic airway diseases.

側腦室內 Neostigmine이 家兎 腎臟機能에 미치는 影響

李載欣 충남대학교 대학원 1976 論文集 Vol.6 No.-

Effect of neostigmine administered intracerebroventricularly on the renal function of rabbits was investigated. 1) Intraventricular neostigmine induced decrease after initial increase of urine flow and changes of renal plasma flow, glomerular filtration and urinary sodium and potassium excretion in proportion to urine flow approximately, but did not influence to filtration fraction and reabsorption of sodium and potassium. 2) Correlation coefficient between changes of two parameters among urine volume, renal plasma flow, glomerular filtration and urinary sodium excretion was statistically significant. 3) Increasing changes of various parameters of renal function induced by intraventricular neostigmine were inhibited by intravenous atropine pretreatment and decreasing changes of the parameters partially inhibited by the blocking agent Pretreatment with intravenous regitine potentiated diuretic action and inhibited antidiuretic action of intraventricular neostigmine. Front the above results, it is suggested that the effect of intraventricular neostigmine on the renal function of rabbit is due to renal hemodynamic changes by central parasympathetic and sympathetic activation

正常血壓白鼠와 單一腎臟 및 腎動脈結紮高血壓白鼠에서의 心筋 Na^+, K^+-ATPase에 關한 硏究

李載欣,沈雲澤 충남대학교 의과대학 지역사회의학연구소 1984 충남의대잡지 Vol.11 No.2