Reconstruction of Rabbit Corneal Epithelium on Lyophilized Amniotic Membrane Using the Tilting Dynamic Culture Method

Ahn, Jae-Il,Lee, Doo-Hoon,Ryu, Yang-Hwan,Jang, In-Keun,Yoon, Mun-Young,Shin, Youn Ho,Seo, Young-Kwon,Yoon, Hee-Hoon,Kim, Jae-Chan,Song, Kye-Yong,Yang, Eun-Kyung,Kim, Ki-Ho,Park, Jung-Keug Blackwell Publishing Inc 2007 Artificial Organs Vol.31 No.9

<P>Abstract: </P><P>Rabbit corneal epithelium was reconstructed using tilting dynamic culture with a self-manufactured, amniotic membrane (AM) supporter and a lyophilized amniotic membrane (LAM). Rabbit corneal epithelial (RCE) cells were cultured and cryopreserved after isolation from the limbus. The second- and third-passage RCE cells were plated onto the epithelial side of the LAM of Ahn's AM supporter. Two days later, the air–liquid interface culture was maintained with third-passage RCE cells for 6 days and second-passage corneal epithelial cells for 9 days. The average viability of thawed RCE cells, assessed using trypan blue dye exclusion, was 77.42%. The reconstructed corneal epithelium was characterized by histological (hematoxylin and eosin) and immunohistochemical staining (proliferating cell nuclear antigen) for light microscopy, and by reverse transcriptase-polymerase chain reaction, glucose assay, and transmission electron microscopy. The basal layer of the reconstructed corneal epithelium was well formed, and the epithelium was tightly constructed due to the increase in cell proliferation and differentiation caused by the tilting dynamic culture, as opposed to static culture. Tilting dynamic culture was useful for the reconstruction of the epithelium using easily damaged epithelial cells and resulted in more stratum cell layers. Moreover, cytokeratin (CK3) mRNA expression in tilting dynamic cultured third-passage RCE cells seeded onto AM was greater than in static cultured third-passage RCE cells. The morphology of the reconstructed corneal epithelium on LAM by tilting dynamic culture for 9 days resembled that of the skin epidermis. This was thought to be because the tilting dynamic culture not only accelerated the proliferation and differentiation of cells by physical or mechanical stimulation, but also ensured that the supply of medium was delivered to the basal cells more efficiently. Thus, the reconstruction of the corneal epithelium using LAM and tilting dynamic culture was considered to be a good in vitro model for autologous or allogeneic transplantation of corneal epithelium and skin epidermis in patients with damaged epithelia. </P>

항정신병약물 사용 중인 정신분열병 환자에서 올란자판으로의 교체 방법에 관한 연구(II) : Comparison of Safety 안전성 비교

안용민,권용실,권준수,민성호,박두병,양문정,소형석,송종호,신윤식,우행원,유범희,이홍석,정한용,한창환,김용식 大韓神經精神醫學會 2002 신경정신의학 Vol.41 No.5

연구목적: 이 다기관 공동임상연구는 사용 중인 항정신병약물을 ’직접 교체 방법’또는 ’시작-감량 교체 방법’중 한 가지 방법으로 올란자핀으로 교체한 후, 안정성 측면에서 두 교체 방법 간의 비교와 교체후의 변화를 관찰하기 위한 것이다. 방법: 국내 13개 병원의 입원 및 외래에 내원한 환자들 중 ICD-10 지단기준으로 정신분열병에 해당되며, 임상적으로 항정신병약물 교체가 필요한 환자를 대상으로 하였다. 두 가지 교체 방법 중 한 가지를 무작위로 피험자에 적용하였으며, ’직접 교체 방법’에 배정된 경우에는 사용중인 항정신병약물을 일시에 중단하고 10㎎의 올란자핀을 바로 투여하였고, ’시작-감량 교체 방법’에 배정된 경우는 10㎎의 올란자핀 투여하고 2주에 걸쳐서 기존 약물을 감량하여 중단하였다. 올란자핀 사용기간은 총 6주이며, 용량은 5∼20㎎ 범위로 제한하였다. 한정성 평가를 위해서 체중, 생명징후, 자발적인 이상반응 복, 실험실 검사 그리고 Simpson-Angus Scale(SAS), Barnes akathisia rating scale(BARS), Abnormal involuntary movement scale(AIMS). Liverpool University neuroleptic side effect rating scale(LUNSERS)등을 이용하였다. 결과: 총 103명의 정신분열병 환자를 대상으로 하였다. 사용한 올란자핀의 용량, 벤조디아제핀의 병용률, 탈락률과 탈락 사유, 자발적인 이상반응 보고, 생명징후, 실험실 검사 그리고 대부분의 부작용 척도 상에서 임상적으로 의미 있는 차이를 두 교체 방법간에 발견하지 못하였다. 다만 AIMS의 감소는 ’직접 교체 방법’군에서 보다 적었고, 항콜린제의 병용률은 ’시작-감량 교체 방법’군에서 보다 많았다. 기저 상태에서 전체 피험자의 SAS와 BARS 점수는 각각 3.5점과 1.8점이었으며 70% 이상의 피험자가 고프로락틴 혈증을 보였다. 올란자핀으로 교체한 후, SAS, BARS, AIMS 점수의 유의한 감소가 있었으며 고프로락틴 혈증을 보인 피험자 분율도 약 30%이하로 감소하였다. 그러나 교체 방법과 상관없이 올란자핀 교체 후 유의한 체중 증가가 있었다. 결론: 이 연구를 통해 교체 방법에 관계없이 비교적 안전하고 용이하게 올란자핀으로 교체 할 수 있음을 알 수 있었다. 그리고 기존 항정신병약물을 올란자핀으로 교체함으로써 일부 부작용들을 줄일 수 있음을 간접적으로 관찰할 수 있었다. 하지만 이 연구는 여러 제한점과 문제점을 지니고 있기 때문에 보다 체계적인 연구를 통해 검정이 필요하리라 생각된다. Objectives: This multicenter clinical trial involving 13 hospital sites compared the safely of switching to olanzapine between ’direct switching method’ and ’start-tapering switching method’. Method: This study included both inpatients and outpatients who fulfilled the criteria for schizophrenia as defined in the ICD-10, and were in need to be appropriate for switching antipsychotics. Subjects were randomly assigned to one of the two switching methods. For ’direct switching method’group, previous antipsychotics were abruptly discontinued and 10㎎ of olanzapine was administered, and previous antipsychotics was gradually tapered for 2 weeks. Olanzapine was used for 6 weeks and the dose was adjusted within the range of 5-20㎎. The safety of switching to olanzapine was measured with vital sings including body weight, adverse events reported spontaneously, laboratory tests, and various scales such as Simpson-Angus Scale(SAS), Barnes Akathisia Rating Scale(BARS). Abnormal Involuntary Movement Scale(AIMS), and Liverpool University Neuroleptic Side Effect Rating Scale(LUNSERS). Results: 103 patients were switched to olanzapine in this study. The comparison between two switching methods did not show any significant difference in the dosage of olanzapine used, the concomitant use of benzodiazepine, the rate and reasons of drop-out, the adverse events, vital signs, laboratory tests, and most scales for measuring side-effects. However, the decrease in AIMS scores was significantly lower in ’direct switching method’ group, and the concomitant use of anticholinergics was comparatively greater in ’start-tapering switching method’ group. At baseline, SAS and BARS scores were 3.5 and 1.8 points respectively, and more than 70% of the subjects showed hyperprolactinemia. After switching to olanzapine, SAS, BARS, and AIMS scores were significantly decreased and the proportion of the patients with hyperprolactinemia was also decreased to less than 30%. However significant weight gain after the treatment of olanzapine was observed regardless of switching method. Conclusion: This study may suggest that switching to olanzapine can be done with relatively high safety regardless of switching methods and olanzapine can significantly decrease some side-effects induced by other antipsychotics.

A Comparison of Lyophilized Amniotic Membrane with Cryopreserved Amniotic Membrane for the Reconstruction of Rabbit Corneal Epithelium

Ahn Jae-Il,Jang In-Keun,Lee Doo-Hoon,Seo Young-Kwon,Yoon Hee-Hoon,Shin Youn-Ho,Kim Jae-Chan,Song Kye-Yong,Lee Hee-Gu,Yang Eun-Kyung,Kim Ki-Ho,Park Jung-Keung The Korean Society for Biotechnology and Bioengine 2005 Biotechnology and Bioprocess Engineering Vol.10 No.3

Many researchers have employed cryopreserved amniotic membrane (CAM) in the treatment of a severely damaged cornea, using corneal epithelial cells cultured on an amniotic membrane (AM). In this study, two Teflon rings were made for culturing the cells on the LAM and CAM, and were then used to support the AM, which is referred to in this paper as an Ahn's AM supporter. The primary corneal epithelial cells were obtained from the limbus, using an ex-plantation method. The corneal epithelium could be reconstructed by culturing the third­passage corneal epithelial cells on the AM. A lyophilized amniotic membrane (LAM) has a higher rate of graft take, a longer shelf life, is easier to store, and safer, due to gamma irradiation, than a (AM. The corneal epithelium reconstructed on the LAM and (AM, supported by the two­Teflon rings, was similar to normal corneal epithelium. However, the advantages of the LAM over that of the (AM make the former more useful. The reconstruction model of the corneal epithelium, using AM, is considered as a good in vitro model for transplantation of cornel epithelium into patients with a severely damaged cornea.

정신분열병 및 정신분열형 장애에 대한 올란자핀의 효과와 안전성

안용민,강대엽,권준수,김창윤,김철응,반건호,신영민,이기철,이동우,이중서,조현상,채정호,김용식 大韓神經精神醫學會 2001 신경정신의학 Vol.40 No.4

연구목적 : 본 다기관 공동임상연구는 국내 환자를 대상으로 새로운 항정신병약물인 올라자핀의 치료 효과와 안전성을 확인하고자 시행되었다. 방 법 : 1999년 7월부터 2000년 3월까지 국내 10개 병원에 방문한 정신분열병 및 정신분열형 장애 105명을 대상으로 비대조 개방 임상연구를 시행하였다. 중등도 이상의 정신병 증상을 지니거나, 부작용으로 약물교체가 필요한 경우 또는 기분장애 증상의 치료가 필요한 환자를 대상으로 하였다. 2∼7일간의 약물 배설기간 후에 1일 10mg의 올란자핀을 투여하였고, 이후로 3∼7일 간격으로 용량을 조절하여 총 8주간 투여하였다. 치료 효과는 PANSS(Positive and Negative Syndrome Scale), BPRS(Brief Psychiatric Rating Scale), CGI(Clinical Global Impression-Severity), MADRS(Montgomery-Asberg Depression Rating Scale), HAM-A(Hamilton Rating Scale for Anxiety)로 판정하였다. 안전성 평가는 자발적인 이상반응 보고, 활력징후와 혈액 검사 및 SAS(Simpson-Angus Scale), BARS(Barnes Akathisia Rating Scale), AIMS(Abnormal Involuntary Movement Scale)척도를 이용하였다. 결 과 : 105명중에서 85.7%인 90명이 8주간의 치료를 완료하였다. 평균 최빈 용량은 일일 16.1(±4.7)mg이었고, 종료시점에서의 BPRS전체 점수가 기저 상태에 비해 40%이상 감소된 반응군은 69.5%이었다. PANSS의 양성 증후군과 일반정신병리 뿐만 아니라 음성 증후군 소척도에서도 유의한 감소를 보였으며, 이 감소들은 치료 초기부터 나타나서 8주간 지속되었다. 43.8%의 피험자가 중등도 이상의 우울증상을 같이 지니고 있었으며, 올란자핀에 의해 MADRS와 HAM-A가 유의하게 감소하였다. 치료 기간 동안에 활력징후에는 뚜렷한 변화가 없었으나 체중은 8주간 지속적으로 증가하였다. SAS와 AIMS는 감소하였으며, 정좌불능증을 제외한 나머지 추체외로 증상의 발생비율도 낮았다. 임상적인 증상이나 징후를 동반하지 않는 ALT/SGPT의 상승을 보였지만 프로락틴을 포함하는 대부분의 혈액 검사상 뚜렷한 이상 변화는 없었다. 결 론 ; 비록 본 임상연구가 비대조 개방 임상연구로서 많은 제한점을 가지고 있지만, 올란자핀이 기존 약물에 내약성을 보이거나 기분증상을 동반하는 정신병에 치료 효과가 있었다. 그리고 기존 항정신병약물 치료시에 문제가 되었던 추체외로 증상과 고프르로락틴 혈증을 포함하는 대부분의 부작용면에서 안전성을 보여 주었다. Objective : This multicenter clinical trial was carried out to investigate the efficacy and the safety of olanzpine for the treatment of Korean patients. Mothod : 105 patients with schizophrenia and schizophreniform disorder, visited at 10 mental or university hospitals, had received an open and non-comparative treatment with olanzapine for 8 weeks. Patients had psychotic or depressive symptoms with the severity above moderate degree or intolerable side effects to previous antipsychotics. After a wash-out period of 2-7 days, 10mg olanzapine was prescribed initially to all the patients, and then the dosage could be adjusted within the range of 5-20mg/day of olanzapine by 3-7 days. Results : 90(85.7%) of 105 patients completed the 8-weeks trial and the mean modal dose of olanzapine was 16.1(±4.7%)mg/day. At the end of the trial, 73 patients(69.5%) were classified as responder, which was defined as 40% or more improvement in BPRS(Brief Psychiatric Rating Scale) score comparing to baseline. There was a significant reduction in the scores of PANSS(Positive and Negative Syndrome Scale)and subscales including negative symptom scores and CGI. Also weekly analysis showed that the reductions in scores were kept on for the whole period of the trial. 43.8% of all the patients had depressive symptoms at the baseline and total scores of MADRS(Montgomery-Asberg Depression Rating Scale) and HAM-A(Hamilton Rating Scale for Anxiety) were also reduced after the trials. Vital signs revealed no clinically significant changes but continuous weight gain was observed during the treatment with olanzapine. The scores of SAS(Simpson-Angus Scale) and AIMS(Abnormal Involuntary Movement Scale) for assessing the EPS(extrapyramidal symptoms)and tardive dyskinesia respectively were significantly decreased and only a few patients reported EPS as adverse events. Although mild and clinically non-significant of ALT/SGPT was observed, most laboratory parameters including plasma prolactin level showed to significant changes during the trial. Conclusions : Although this trial had many limitations because it was a non-comparative and open study, olanzapine showed high efficacy on the positive, negative and depressive symptoms in schizophrenia and schizophreniform disorder.In addition to that, olanzapine showed a substantially favorable safety profile, such as low incidence of EPS and hyperprolactinemia.

장거리 달리기 선수에서 횡문근융해와 생화학적 지표의 변화에 대한 연구

안선호,최석채,김태현,나용호,송주흥,이영진,조지현,최석준 대한신장학회 2000 대한신장학회잡지 Vol.19 No.5

작업관련성 근골격계질환으로 요양승인된 사례 분석

안연순,최용휴,강성규,정호근 大韓産業醫學會 2002 대한직업환경의학회지 Vol.14 No.2

목 적 : 이 연구는 1999년 근로복지공단에서 업무상질병으로 인정한 근골격계질환을 정밀분석하여 질병의 종류와 특성을 밝힘으로서 직업성 근골격계질환 예방사업이나 업무상질병 판정의 기초자료로 제공하고자 실시하였다. 방 법 : 근로복지공단 주전산망을 통하여 재해일자가 1999년 1월 1일부터 12월 31일까지인 근로자중 2000년 6월 30일까지 요양이 승인된 업무상 질병자 2,333명을 파악하고 이 중 뇌심혈관계질환자 1,348명과 직업병자 575명을 제외한 근골격계질환자 410명을 연구대상으로 하였다. 소속 사업장 또는 연구대상 근로자에 대하여 우편 또는 전화 면담조사를 실시하여 업무상 질병자의 성, 연령, 질병 관련 작업기간, 질병 발생원인, 업종, 직종, 질병종류, 사업장규모 등에 대한 조사를 실시 후 기술분석을 시행하였다. 결 과 : 근골격계질환자 410명 중 남성이 310명(75.6 %), 여성이 100명(24.4 %)이었다. 연령별로는 30-39세가 143명(34.9 %)을로 가장 많았고, 질병관련 근무기간은 1년 이상 5년 미만 근무자의 비율이 36.0 %로 가장 높았다. 소속 사업장 규모는 5인 이상 50인 미만 사업장이 38.0 %로 가장 높았다. 종사업종은 제조업이 235명(57.3 %)을로 가장 많았고 다음으로 운수업 46명(11.2 %), 건설업 25명(6.1 %) 순 이었다. 제조업 중에는 자동차 밑 트레일러 제조업과 운송장비제조업이 각각 58명, 35명으로 가장 많았다. 직종별로는 장치, 기계조작 및 조립 종사자가 129명(36.5 %)을로 가장 많았고 다음으로 단순 노무 종사자 90명(25,5 %), 기능원 및 관련기능종사자 63명(17.8 %), 서비스 종사자 28명(7.9 %) 순 이었다. 질병별로는 전체 424건 중 요부질환이 232건(54.7 %)이었고, 경부 및 상지질환이 183건(43.2 %), 하지질환이 9건(2.1 %)이었다. 요부질환자의 평균 연령은 36.5세, 평균작업기간은 4.7년이었고, 질병 종료는 요추간판탈출증이 134건(57.5 %)을로 가장 많았다. 질병 발생원인은 반복작업에 의한 경우가 54건, 비반복성 원인에 의한 경우가 89건이었는데 반복작업은 부적절한 작업자세로 인한 요통이 23건으로 가장 많았고, 비반복성 원인은 들기작업에 의한 요통이 27건으로 가장 많았다. 결 론 : 이 연구는 재해발생 시점과 조사시점이 많은 차이가 있어 근골격계질환과 관련하여 중요한 많은 항목들을 제대로 조사하지 못하였고, 특히 발생 원인은 약 70%만 조사가 가능하여 결과가 선택치우침에 의해 도출되었을 가능성을 배제할 수 없다. 그러나, 이 연구를 통하여 우리나라에서 업무상질병으로 인정받은 근골격계질환을 분석함으로써 연령, 성, 작업기간 등 질병 발생 근로자의 특성과 고위험업종 및 직종, 발생 원인 등을 전체적으로 파악할 수 있었던 것은 나름대로 의의가 있다. Objectives : The purpose of this study was to analyze the characteristics of work-related musculoskeletal diseases, as listed by the Korea Labor Welfare Corperation(KLWC) Methods : Using the database of the KLWC, we collected information from 410 approved occupational musculoskeletal disease cases, which occurred between 1 January and 31 December 1999 and were approved by 30 June in 2000. We investigated the characteristics of the disease using a mail survey and telephone interviews. The characteristics we investigated included : sex, age, disease-related working duration, process of disease occurrence, type of enterprises, etc. Results : Men accounted for 75.6 % (310 workers) of the approved cases. The most common age group was 30-39 years (n=143, 34,9 %). The mean disease-related working duration was 5.7 years, and the most commonly had a work-duration of 1-5 years (n=108, 36.0 %). The major industrial types of enterprise were manufacturing (235 workers, 57.3 %), transportation (46 workers, 11.2 %), and construction (25 workers, 6.1 %). Within the manufacturing industry, the most common was motor vehicles and trailers manufacturing. Within the group, the most common jobs were plant or machine operators and assemblers (129 workers, 36.5 %), elementary occupations (90 workers, 25.5 %), and craft and related trades workers (63 workers, 17.8 %). The majority of the diseases involved lumbar problems (232 cases, 54,7 %), neck and upper extremity diseases (183 cases, 43.2 %) and lower extremity diseases (9 cases, 2.1 %). In the cases of lumbar disease, the mean age and working duration was 36.5 years and 4.7 years, respectively. Fifty-four cases of lumbar diseases were due to repetitive work and 89 due to non-repetitive work. Bad posture at work was the most common cause for those doing repetitive woek(23 cases), and lifting the most common cause of problems in the non-repetitive work cases (27). Conclusions : We were able to elucidate the characteristics of workers with approvd occupational musculoskeletal disease through this study. Many approved cases occurred in women, and workers performing repetitive work, of these herniated nucleus pulposus occurred in many workers performing simple repetitive works without serious external forces. This suggests that a prevention policy for occupational musculoskeletal disease must be established, to focusing on the above-mentioned high-risk groups.

한국인에서 DXS7132 유전좌와 GATA31D10 유전좌의 다형성에 관한 연구

안종성,장영길,이숭덕,신창호,이윤성,이정빈 大韓法醫學會 2000 대한법의학회지 Vol.24 No.1

The validation study for two STR loci on X-chromosome, DXS7132 and GATA31D10, was done including allelic distribution and frequency of each allele to use these results for individual identification and paternity testing. For 496 unrelated Koreans, above two STR loci were amplified simultaneously using duplex PCR amplification method. The amplified products were analyzed by polyarylamide gel electrophoresis followed by silver staining. In male DXS7132 locus revealed 7 different alleles ranging from 276bp to 300bp. The largest allele was consisted of 14 repetition of [TCTA] unit and took 0.3417. The allele 15 followed next as 0.3165 and allele 13 as 0.1726. In female general distribution was same except one allele, allele 18 was found additionally. The heterozygosity was 0.7706 and 23 different genotypes were found. Polymorphism information content(PIC) was 0.727. Two cases of mutation were noted in DXS7132 locus In both male and femal 7 different alleles were noted in GATA31D10 locus and the alleles ranged from 195bp to 231bp. The allele 15(199bp) took the majority of all as 0.825. The other alleles showed rather relatively low frequency. The heterozygosity was 0.2385 and 11 different genotypes were found. PIC was 0.2521, and no mutation was noted in GATA31D10 locus. Considering these two loci together, 22 different halpotype were noted.

자발성 기흉을 동반한 폐림프관평활근종증 1예

안용환,정종훈,김소영,김휘정,김학렬,양세훈,최순호,문형배,조향정,정은택 圓光大學校 醫科學硏究所 2005 圓光醫科學 Vol.20 No.1

외상성 횡격막 손상

안성국,이상목,이기형,고석환,김용호,박호철,고영관,조규석 대한응급의학회 1995 대한응급의학회지 Vol.6 No.1

Fifty two cases of traumatic diaphragmatic injuries that we have experienced from Jan. 1973 to Oct. 1994 were evaluated. The following results were obtained. The age of the patient was ranged from 1 to 74 years. Male was 38 and female was 14 in number with a ratio of 2.7 : 1. The traumatic diaphragmatic injuries were due to blunt trauma in 35 cases(motor vehicle accident 25, fall down 8, press 1, kick by fight 1) and penetrating trauma in 17 cases(stab wound 15, shot wound 1, explosion 1). In the blunt trauma, the preoperative diagnosis of the diaphragmatic injury was possible in 25 out of 35 cases(71%) and in the penetrating trauma, 15 out of 17 cases(88%). In the blunt trauma, the repture site was located in the left in 22 cases(63%) and in the right in 13 cases(31%). In the penetrating trauma, the rupture site was located in the left in 9 cases(53%) and in the right in 8 cases(47%). In the blunt trauma, 20 cases(63%) were treated within 24 hours and in the penetrating, 15 cases(88%) within 24 hours. In the blunt trauma, the herniated organs into the thorax were stomach(7), omentum(6), spleen(6), liver(5), colon(4), small bowel(2) and in the penetrating, stomach(7), colon(6), omentum(3), liver(2), and spleen(1) were herniated. Injury severity score(ISS) of 35-blunt trauma ranged from 11 to 66 with mean value of 30.6. Mean ISS of survivors and nonsurvivors was 27.6 and 52.7 respectively. The diaphragmatic repair of 49 cases was performed with thoracic approach in 23 cases, thoracoabdominal approach in 7 cases and abdominal approach in 19 cases, and 3 cases were not operated. The postoperative complication and mortality were developed in 16 out of 49 cases(33%) and in 5 cases(9.6%) respectively, and the causes of death were hypovolemic shock(1), combined head injury(2), asphyxia(1), and pulomnary edema and renal failure(1). In conclusion, the injuries of the diaphragm should be suspected in all patients with severe blunt trauma or penetrating injuries at thorax and upper abdominal area near the diaphragm. All of the cases had associated injury and most of deaths were related to the severity of associated injuries.

上向流式 好氣性 生物膜에 의한 農藥含有廢水의 處理에 관한 硏究

안용희,장성호,안종수,박출재,김수생 동아대학교 공과대학 부설 한국자원개발연구소 1994 硏究報告 Vol.18 No.2

This study has been carried out to determine the toxicity of agricultural chemical (TPN) as to wastewater treatment. This system used Aerated Submerged Up-Flow Biofilm Reactor. This reactor can withstand the variation of concentration, flow rate and presence of certain toxic materials in the wastewater to be treated. An acclimation experiment was variated influent COD from 250㎎/ℓ~70㎎/ℓ. The agricultural chemical was added to obtained optimum condition at influent COD 400㎎/ℓ by the acclimation experiment. The conclusions from the experimental results are as follows ; 1. Influent COD concentration was variated 250㎎/ℓ~600㎎/ℓ. The treatment efficiency was stability(86% to 93.3%). When influent COD was 400㎎/ℓ, the treatment efficiency was at best-93.2%. Accordingly, this system was proved to be suitable in treating the wastewater with wide range of concentration. 2. The influent TPN concentration below 1ppm at Up-Flow Aerated Biofillm Reactor, raised no harmful result to treatment efficiency. 3. The treatment efficiency was decreased to 29.8% when the lapse of time. 4. When the experimental TPN toxicity was compared with the DDT toxicity in the reference, it was proved that both of the toxicity had the similer trend.