Bupivacaine과 ropivacaine이 Xenopus oocyte에 발현된 HERG 전류에 미치는 영향

김국성,이규승,김효신,손숙진,이상도,김광진,전병화,김윤희,박진봉 충남대학교 의과대학 의학연구소 2003 충남의대잡지 Vol.30 No.1

Bupivacaine is an amide-type local anesthetic widely used for regional anesthesia. Ropivacaine is developed as a less cardiotoxic alternatives to bupivacaine. In the present study, we have analyzed the effects of bupivacaine and ropivacaine on HERG currents expressed in Xenopus oocytes. Bupivacaine and ropivacaine(3∼1,000μM) blocked HERG currents in a concentration dependent manner. EC_(50) was 26.1±3.1μM(n_(R) 0.65±0.04) and 43.5±7.9μM(n_(H) 0.99±0.13) in bupivacaine and ropivacaine, respectively. Bupivacaine and ropivacaine did not affect the activation and deactivation kinetics of HERG channels. However, the drugs decreased the slope conductance measured from fully activated current-voltage relationship curves. These results suggest that bupivacaine and ropivacaine have a similarinhibitory effect on HERG channels, which could be a possible cellular mechanism of LQT or ventricular arrythmia by the drugs.

NSIP와 BOOP의 고해상전산화단층촬영 소견과 비교

백상현,박재성,차장규,홍현숙,김대호,박성진,조준희,이혜경,최진수 순천향의학연구소 2003 Journal of Soonchunhyang Medical Science Vol.9 No.2

Purpose: BOOP and NSIP also share similar clinical features such as subacute flu-like illness, the finding of bronchoalveolar lavage, the result of pulmonary function test and more favourable outcome than usual interstitial pneumonia(UIP). The author try to compare the HRCT findings of NSIP with BOOP by pattern and distribution of diseases. Materials and Methods: HRCT findings of histopathologically proven NSIP(Group Ⅰ; 6, Group Ⅱ; 7, Group Ⅲ; 2) in 15 patients(5 male, 10 female, age range, 39-69 years; mean age, 53 years), BOOP in 15 patients(6 male, 9 female, age range, 26-76 years; mean age, 52 years) were reviewed retrospectively. Results: The common pattern of the two diseases was admixed consolidation, ground glass attenuation & reticular densities with/without architectural distortion(NSIP; 53.3%, BOOP; 46.7%). The main component of the pattern wan predominantly consolidation(NSIP; 46.7%, BOOP; 73.3%) and ground glass attenuation(NSIP; 40.0%, BOOP;20.0%). Architectural distotion was more provinent finding with NSIP(mild ; 3, moderate ; 4, severe; 2) compared with BOOP(mild; 7, moderate; 2, severe; 0). The predominant overall distribution was lower lung, peribronchovascular & peripheral lung field in both of the two entities(NSIP;73.3%, BOOP; 73.3%). Peribronchovascular involvement was more common with NSIP(100%) than BOOP(80%), whereas peripheral involvement was more common with BOOP(93.3%) than NSIP(80%). Conclusion: The HRCT findings of NSIP and BOOP were similar in pattern and distribution. But NIP manifested more frequently ground-glass attenuation, whereas BOOP showed more frequently consolidation. Architectural distortion was more severe with NSIP than BOOP. NSIP invoved more frequently peribronchovascular space and BOOP were more common in peripheral lung field.

컴퓨터 교육에서 정보윤리 교육을 위한 교육과정 모델 설계

임진숙,구정모,김성식 한국컴퓨터교육학회 2004 컴퓨터교육학회 논문지 Vol.7 No.2

초등학교 컴퓨터 교육의 필수화로 그동안 다른 과목에 흡수되었던 초등학교 컴퓨터가 독립 과목형태로 교육되고 있으나, 이는 ICT소양교육의 내용 체계를 준거로 하고 있어서 중등학교 컴퓨터 교육과정과의 비연계성 면에서 문제가 제기되고 있으며, 컴퓨터 교육의 한 부분인 정보윤리교육도 같은 문제를 가지고 있다고 할 수 있다. 정보윤리교육의 이러한 문제를 해결하기 위해서는 초·중등학교 전체 과정을 통하여 교육되어야 할 정보윤리교육 목표를 설정하고 내용체계를 재조직할 필요가 있다. 이에 따라 본 연구에서는 현재 시행되고 있는 ICT소양교육과 컴퓨터 교육과정에 포함된 정보윤리교육 내용 체계와 미국의 컴퓨터 교육과정에 포함된 정보윤리 관련 내용 체계를 분석하고, 이러한 분석에 기초하여 컴퓨터 교육에서 정보윤리교육을 위한 교육과정 모델을 설계하였다. After the computer education become essential in elementary school, computer subject which was absorbed by the other subjects is educating in independent way. However, since the contents of computer education in elementary school is based on the ICT literacy education, sequential disconnection problem appears between elementary school and secondary school computer education curriculum. Therefore, information ethics education that is one part of computer education also has same problems. To solve these problems of information ethics education, it is necessary to set the goal of information ethics education that should be educated through the whole courses of elementary and secondary school and to reorganize the contents. In this study, we analyzed the contents of information ethics education included in ICT literacy education and computer curriculum, and analyzed the contents of information ethics education included in U.S. computer curriculum. Based on these analysis, we designed a curriculum model for information ethics education in computer education.

배급수계통에서 세균에 의한 수돗물의 2차 오염

방숙진,박성주 대전대학교 기초과학연구소 1998 自然科學 Vol.9 No.1

대청호 원수를 사용하는 청주정수장으로부터 배수지, 가정 저수조, 수도관을 통과하는 수돗물을 채취하여 1996년 3월부터 10월까지 세균의 분포와 물리화학적 환경요인을 조사하였다. 평균 유리 잔류염소 농도는 배급수계통의 거리에 반비례하여 현저히 감소한 반면 평균 총세균수(AODC)법)는 1.06×10??∼1.33×10?? cells/㎖, 종속영양세균수(R2A법)는 5.79×10²∼1.09×10³CFU/㎖로 비교적 일정하였다. 그러나 수온이 20℃ 이상으로 증가하고 저수량이 현저하게 감소한 5월에 배수지의 수돗물에서는 유리잔류염소가 0.5㎎/ℓ이상을 유지함에도 불구하고 종속영양세균과 총대장균군 재생장에 의한 2차 오염이 발생하였다. 분변성 대장구균과 분변성 연쇄상구균의 재생장애에 의한 수돗물의 2차 오염은 잔류염소가 현저히 감소한 관말지점에서 주로 발생하였다. 선택배지에서 자란 Salmonella와 Shigella의 전형적인 추정 양성 콜로니를 동정한 결과 진성은 하나도 없었다. 대장균군 선택배지에서 생장한 대부분의 추정 양성 콜로니는 Enterobacteriaceae 과에 속하는 Enterobacter, Klebsiella, Serratia, Erwinia, Hafnia 속으로 동정되었다. Bacterial densities and physico-chemical factors of drinking waters from distribution reservoirs and a domestic storage tank and tap waters through the distribution system of Chongju Water Treatment Plant where Daechong Reservoir waters are used as raw water were monthly analyzed from March to October in 1996. Mean free chlorine residuals gradually decreased against distance of the distribution system, whereas mean total bacterial numbers by AODC method and mean heterotrophic plate count bacteria by R2A method were generally constant regardless of distance of the system, 1.06×10?? to 1.33×10?? cell/㎖ and 5.79×10²to 1.09×10³ CFU/㎖, respectively. However, the secondary pollution of drinking waters by heterotrophic plate count and total coliform bacteria occurred in the water distribution reservoirs especially in May when water significantly reduced, although free chlorine residuals were 0.5㎎/ℓ or more. The secondary pollution by fecal coliforms and fecal streptococci also occurred mainly in water pipe ends where chlorine residuals were quite low. None of presumptive positive colonies of Salmonella and Shigella grown on selective media were identified as real positive ones. Most of presumptive total and fecal coliform colonies developed on selective media belonged to the genera of Enterobacter, Klebsiella, Serratia, Erwinia, or Hafnia of the family of Enterobacteriaceae.

2.9% 과산화수소를 함유한 부착형 미백제의 임상적 효능과 안정성에 관한 연구

박은숙,성소래,홍성태,김지은,이소영,황수연,이신재,진보형,손호현,조병훈 大韓齒科保存學會 2006 Restorative Dentistry & Endodontics Vol.31 No.4

2.9%의 과산화수소를 함유한 시험용 부착형 미백제(Medison dental whitening strip, Samsung medical Co.,Anyang,Korea)를 실험군 피험자 23명, 시험용 미백제에서 과산화수소를 제외한 동일한 제재를 대조약으로 대조군 피험자 24명에게 매일 1시간 30분씩, 2주간 적용하여 임상적 효능과 안전성을 평가하였다. 미백 효과는 Shade Vision, Chroma Mete 및 Vitapan classical shade guide를 이용하여 색변화를 측정하여 다음과 같은 결론을 얻었다. 1. 실험군에서의 전체 치아의 색변화량(ΔE^(*))은 세 가지 측정법에서 모두 대조군에 비해 통계적으로 유의한 차이를 보였고 (p < 0.05). 쉽게 인지할 수 있는 정도였다. 2. 색변화는 색좌표 상에서 명도의 증가와 녹색조 및 청색조로의 변화에 의해 얻어졌다. 3. 치아별로는 어두운 견치의 색변화량이 가장 컸고, 측절치, 중절치 순으로 감소하였다. 4. 47명의 피험자 중 5명이 경도의 과민증을 경험하였으나 곧 완화되었다, 치은염증지수와 전기치수검사 결과 시험 전후에 차이가 없어 안전성을 확인하였다. This study evaluated the effectiveness and safety of an experimental bleaching strip (Medison dental whitening strip, Samsung medical Co., Anyang, Korea) containing 2.9% hydrogen peroxide. Twenty-three volunteers used the bleaching strips for one and a half hour daily for 2 weeks. As control group, the same strips in which hydrogen peroxide was not included were used by 24 volunteers with the same protocol. The shade chang (ΔE^(*), color differnce) of twelve anterior teeth was measured using Shade Vision (X-Rite Inc., S. W. Grandville, MI, USA), Chroma Meter (Minota Co., Ltd. Osaka, Japan) and Vitapan classical shade guide (Vita Zahnfabrik, Germany). The shade change of overall teeth in the experimental group was significantly greater than that in the control group (p < 0.05) and was easily perceivable. The change resulted from the increase of lightness (CIE L^(*) value) and the decrease of redness (CIE a^(*) value) and yellowness (CIE b^(*) value). The shade change of individual tooth was greatest in canine, can smallest in central incisor. The safety of the bleaching strip was also confirmed. 〔J Kor Acad Cons Dent 31(4):269-281, 2006〕

붕어의 발육 및 생식에 미치는 비소의 영향

남성숙,이철우,류지성,박응로,남규찬,류홍일,전성환,나진균,최덕일,박광식 한국환경독성학회 2001 환경독성보건학회지 Vol.16 No.2

To evaluate the arsenic effect of fish endocrine disruption, crucian carp (Carassius auratus) were treated with 0.5 mg/L and 3.0mg/L of As₂O₃ for 14 days and gonadal development was examined by histological analysis. In ovaries from female crucian carps exposed to 3.0mg/L, immature follicles and atretic follicles were appeared while various normal developmental stages of oocytes were shown in control group. Body weight and Gonadosomatic Index (GSI) of treated carp were also decreased compared to control group. However, no other significant histological changes in liver or kidney were shown in this exposure scheme, This means that reproductive organs are more sensitive than other organs and arsenic may exert endocrine disrupting effect through inhibiting the development of reproductive organ in fish.

직장 구조조정과 파업에 의한 근로자의 사회심리적 스트레스의 변화

박봉진,이종태,손혜숙,김성준 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.5

Objective : This study was conducted to evaluate the insecurity of employment and anxiety by downsizing and strikes affected job characteristics and psychosocial distress of workers. Methods and Material : This is a longitudinal study of the container terminal workers in Pusan. The company operated downsizing from October to December in 1999 by economic depression. In the result of that, the number of workers decreased into 627 from 662. During that period, a new union was built and it made conflict between employers and employees, so the conflict caused the strikes from February to April in 2000. The first cross-sectional study was performed in February, 2000 and the oneyear follow-up cross-sectional study was performed in February, 2001. Socio-demo graphic characteristics, stress-related behavior, job characteristics and psychosocial distress were assessed with job content questionnaire(JCQ) and Psychosocial well-being Index(PWI) in 200 workers(white-collar: 100 men, blue collar: 100 men) from 662 men in total. Results : In the first study, job strain was high in the whole, but there was no difference between white and blue-collar workers. The level of PWI was very high at 86.67 points and the level of blue-collar workers was higher than that of white-collar workers(p=0.013). In the results of follow-up study, the rate of drinking and smoking decreased as the change of stress-related behaviors. In the job characteristics change, decision authority and physical exertion of the white-collar workers got lower(p<0.05), but social support and job strain didn't have no difference. In the PWI level change, stress level got rather higher(p<0.01), especially, increase of the white-collar workers' stress was big difference from that of the blue-collar workers'. Conclusion : Job stress management programs should be made to reduce stress. And in approaches to management and prevention, excessive physical exertion, shift work and irregular meals and breaks should be considered. Especially, this stress control program should be begun with recognition on conflicts between employers and employees and between employees and employees by downsizing and strikes. And also efforts to reinforce social support should be needed.

간질발작후 MRI 영상에서 뇌 종창을 보인 3예 보고

홍현숙,김대호,이혜경,박재성,박성진,조준희,차장규,백상현 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

We report seizure induced brain swelling in three patients. MRI revealed a cortical, subcortical(3/3), and basal ganglia(2/3) high signal intensity on T2- weighted and FLAIR images. The lesion shows low signal on T1-weighted image, and enhanced after contrast administration. Follow up study after 27 days in one case revealed complete resolution of the lesion. The reversible MRI findings may be the results of a local brain swelling, breakdown of blood-brain permeability, and a defect of cerebral autoregulation during seizure activity. The transient nature of such findings have to be considered in the differential diagnosis such as reversible ischemia, migraine, inflammation and infection.

대전대학교내 먹는 지하수의 미생물학적 수질 평가

방숙전,안종훈,박성주 대전대학교 기초과학연구소 1997 自然科學 Vol.8 No.1

지하수 원수의 세균수는 수온과는 상관성이 없었으며, 종속영양세균의 경우 수온이 가장 낮았을 때 개체수가 가장 많았다. 원수의 평균 총세균수와 R2A배지에서 자란 종속영양세균수는 각각 6.9x10? cell/㎖, 6.7x10²CFU/㎖ 이었으며, 수도꼭지의 물에서도 세균수의 변화는 별로 없었다. R2A배지에서 종속영양세균수는 PCA배지에서 자란 종속영양세균수에 비하여 대체로 10배 정도 많았는데, 이는 유기물의 농도가 낮고 세균수가 적은 지하수 세균학의 연구에 R2A배지의 사용이 효과적이라는 것을 보여주는 것이다. 지하수 원수에 비하여 수도꼭지의 물에서 세균수가 현저하게 증가된 이른바 급수관에서 재생장 현상을 보인 세균은 종대장균군으로서 원수에서 8 CFU/250㎖로 35배나 증가하였다. 분변성 연쇄상구균도 급수계통에서 심한 재생장 현상이 나타났으며 분변성 대장균둔이 검출되지 않은 물에서도 검출되는 것으로 보아 지하수의 지표세균우로 필요한 것으로 판단된다. Salmonella와 Shigella는 추정시험용 선택배지에서 정형적인 콜로니가 나타나기는 하였지만 동정결과 진성인 것은 하나도 없었다. Bacterial numbers in raw groundwater were not independent of water temperature, rather the highest number of heterotrophic plate count bacteria (HPC) was recorded at the lowest temperature. MEAN numbers of total and HPC bacteria in raw water were 6.9x10? cell/㎖ and 6.7x10²CFU/㎖ respectively, not changnin even in tap water. HPC numbers enumerated by R2A agar media were generally 1 log higher than by plate count agar (PCA) media, showing R2A is preferable to PCA in the study of groundwater bacteriology having low organic matters and bacterial density. Total coliform (TC) densities tremendously increased in tap waters and coliform regrowth in the distribution system occurred. Eight CFU/㎖ of total coliforms in raw water changed to 281 CFU/250㎖ in tap water, 35 times increase in density. Presumptive fecal streptococci (FS) showed regrowth in the distribution system as well, but presumptive fecal coliforms did not. HIgh FS numbers were recorded even in the samples where no or few TC were detected, which represents FS as well as TC are necessary to assess drinking groundwater quality. Even though some typical colonies of Salmonella or shigella were developed on the presumptive selective media, any true positive pathogens were not identified.

Sonographic and Clinical Findings of Surgically Managed Small Bowel Intussuception

홍현숙,이범하,박성진,이해경 순천향의학연구소 2010 Journal of Soonchunhyang Medical Science Vol.16 No.1

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