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Di-2-ethylhexyl phthalate 처리 남아프리카산발톱개구리에서의 vitellogenin 발현
박응로,이철우,류지성,남성숙,전성환,나진균,최덕일,박광식 한국환경독성학회 2001 환경독성보건학회지 Vol.16 No.1
The estrogenic potency of di-2-ethylhexyl phthalate (DEHP) using reverse transcriptase-PCR response of liver vitellogenin mRNA in male African clawed frog (Xenopus laevis) was studied. Male frogs were injected with DEHP at dose of 300 ㎍/kg and 300 mg/kg body weight through the dorsal lymph sac. After 4 days, using suitable pair of RT-PCR primers, vitellogenin mRNA induction in the liver was measured and DEHP showed vitellogenin mRNA induction in only the group treated with 300 mg/kg. Any significant histological abnormalities by the exposure of DEHP was not shown in both testis and liver.
최경민,박응로,주홍신,양재경,이기영,이성택,이무춘,Choi, Kyung-Min,Park, Eung-Roh,Ju, Hong-Shin,Yang, Jae-Kyung,Lee, Ki-Young,Lee, Sung-Taik,Lee, Mu-Choon 유기성자원학회 1996 유기물자원화 Vol.4 No.2
자연계로부터 3종의 광합성 세균 strain KN 1-1, KN 2-1과 KN 2-3을 분리하여 돈분 폐수 처리에 대한 연구를 수행하였다. 유기산이 첨가된 배지에서 광합성 세균의 생육은 유기산을 첨가하지 않은 경우에 비해 2~3배 증가하였으며, bacteriochlorophyll a 함량도 1.5~2배까지 증가함을 보였다. 또한 축산 농가에서 직접 채취한 돈분 폐수에 광합성 세균을 첨가하였을 때 COD 감소율은 KN 1-1인 경우 80%, KN 2-1 89%, KN 2-3 75%를 나타내었다. Photosynthetic bacteria, strains KN 1-1, KN 2-1 and KN 2-3 were isolated from nature, and were studied for swine wastewater treatment. Growth of those photosynthetic bacteria were increased to 2~3 fold in organic-acid added medium(sodium acetate 1g, sodium propionate 1g and sodium butyrate 1g in Lascelles basal medium $1{\ell}$) than cultivation in Lascelles basal medium, and amount of bacteriochlorophyll a were increased to 1.5~2 fold. Chemical Oxygen Demand(COD) in swine wastewater using photosynthetic bacteria, strains KN 1-1, KN 2-1 and KN 2-3 were reduced 80%, 89% and 75%, respectively.
최경민,양재경,박응로,배진우,서용기,이성택,Choi, Kyung-Min,Yang, Jae-Kyung,Park, Eung-Roh,Bae, Jin-Woo,Seo, Yong-Ki,Lee, Sung-Taik 유기성자원학회 1997 유기물자원화 Vol.5 No.1
5-Aminolevulinic acid (ALA) 생합성의 $C_5$ 경로의 전구물질인 L-glutamic acid가 Rhodospirillum rubrum N-1 새포내에서 ALA 생산의 역할을 검토하였다. Lascelles의 기본배지에 L-glutamic acid와 levulinic acid (LA)를 각각 30, 20 mM 첨가배양으로써 균체외 ALA 생산성이 40배 증가(76 mg/l)하였다. 한편 $C_4$ 경로의 기질인 glycine과 succinic acid를 대수기 중기에 각각 60 mM 첨가함으로써, 균의 증식은 억제되었으나 균체외의 ALA는 52 mg/l에 달하였다. The role of L-glutamic acid, a precursor of $C_5$ ALA biosynthetic pathway, on the production of 5-aminolevulinic acid (ALA) has been described in cells of Rhodospirillum rubrum N-1. To the Lascelles basal medium the addition of both 30 mM L-glutamicacid and 20 mM levulinic acid (LA) provided to increase the extracellular ALA yield up to 40 fold (76 mg/l). By the addition of both 60 mM glycine and succinic acid, precursorsof $C_4$ ALA biosynthetic pathway, at middle log phase of cell growth ALA yield was increased 27 fold (52 mg/l) although the celt growth was inhibited to a certain extent.
17$\beta$-Estradiol이 수컷 송사리의 성적 행위 및 생식능력에 미치는 영향
류지성,이철우,박응로,남성숙,남규찬,류홍일,전성환,나진균,최덕일 한국독성학회 2001 Toxicological Research Vol.17 No.2
Sexual behavior and reproductivity of male fIsh were studied as an in vivo screening method of endocrine disruptors. Male medaka (Oryzias latipes) were exposed to 17$\beta$-estradiol at nominal concentrations of 2 and 20 $\mu\textrm{g}$/l for 14 days. After exposure of the chemical, sexual behavior between male medaka and normal female which were injected with prostaglandin $F_{2\alpha}$ just before the test, was analysed by using video camera for one hour. Normal control male showed courtship dancing such as following, guarding, dancing and crossing while 17$\beta$-estradiol treated male did not show any type oj courtship dancing. Furthermore, fecundity and fertility were significantly decreased in the treated group. It was suggested that analysis of sexual behavior could be a useful endpoint for the screening of the endocrine disruptors.
구자민,류지성,정규혁,이철우,박응로,박광식 한국환경독성학회 2001 환경독성보건학회지 Vol.16 No.4
Endocrine disruption in crucian carp (Carassius auratus) living in the branch of Han River were examined. Vitellogenin level in plasma was measured using ELISA system and aromatase mRNA level in brain was observed using RT-PCR technique. In all female fish, vitellogenin levels were in the range of 20-40㎍/ml and aromatase mRNA expression could be detected on the agarose gel after RT-PCR. However, in case of males, vitellogenin level was elevated in only one fish, while vitellogenin was hardly detected in others. Aromatase was expressed in all males although the levels were relatively lower than the level in female fish. Testis-ova and any other histological changes of reproductive organ were not shown in both sexes.
Botryococcus sp.의 생장에 미치는 광도와 영양염류의 영향
오희목,김성빈,박준홍,박응로,이성택,권기석,윤병대 한국산업미생물학회 1997 한국미생물·생명공학회지 Vol.25 No.4
B. braunii UTEX 572는 자연계에서 순수분리된 Botryococcus sp. GE 24에 비하여 25-72 μE/㎡/s의 광조건에서 세포생장이 우수하였다. B. braunii UTEX 572의 비증식속도는 배양 1-9일에 Chu 13 배지에서 0.260 (1/day)로 가장 높았으며, 배양시간이 경과함에 따라 점차 감소하였다. 광도가 25-72 μE/㎡/s범위로 조절된 상태에서 B. braunii UTEX 572는 광도의 증가에 따라 탄수화물 농도, 세포의 총질소와 총인 함량은 감소하지만 단백질 농도와 세포의 N : P ratio는 증가하였다. 엽록소 α농도는 광도가 증가함에 따라 감소하는 일반적인 경향을 따랐다. Chu 13 배지의 초기 pH를 7.5로부터 8.0으로 조절한 경우 배양 35일까지의 건량증가율은 83 ㎎/ℓ/d로 pH 7.0에서의 약 1.3배로 높았다. 배지의 N : P ratio가 50 : 1의 경우 배양 20일에서 배양 28일까지의 건량증가율은 115 ㎎/ℓ/d로 조사된 배양기간 중에서 구간별 최대치를 나타내었다. B. braunii UTEX 572의 open culture시 Chu 13 배지를 사용한 대조구에서 세포생장이 가장 높았으며, pH 7.0 조절, penicillin 250 ㎎/ℓ첨가, glucose 1% 처리 등에 의하여 조류의 생장이 크게 억제되었다. 이것은 자연상태에서 B. braunii UTEX 572의 대량배양이 충분히 가능함을 보이는 결과이다. Under the light intensity of 25-72μE/㎡/s Botryococcus braunii UTEX 572 grew faster than Botryococcus sp. GE 24 isolated from a freshwater lake. The specific growth rate (μ) of B. braunii UTEX 572 was highest at 0.260 (1/day) on a dry weight basis in Chu 13 medium from 1 to 9 days of incubation and then continuously decreased. Carbohydrate concentration and cellular nitrogen and phosphorus contents of B. braunii UTEX 572 gradually decreased with ligh intensity over a range of 25-72μE/㎡/s, whereas the concentrations of protein and cellular N : P ratio increased with light intensity. Chlorophyll-α concnetraton showed a decreasing tendency with light intensity. The dry weight of B. braunii UTEX 572 increased in the highest rate of 83 ㎎/ℓ/day at pH 8.0. When the N : P ratio of Chu 13 medium was adjusted to 50 : 1 by addition of nitrogen source, dry weight increasing rate was 115 ㎎/ℓ/day between 20 and 28 days of incubation which was the highest value during the cultivation. Cell growth in an open culture of B. braunii UTEX 572 was highest with Chu 13 medium, whereas that with Chu 13 medium adjusted to pH 7.0, containing 250 ㎎/ℓ penicillin, or containing 1% glucose was reduced on a large scale. However, this result shows the possibility of the mass cultivation of B. braunii UTEX 572 in an open system competing with other microorganisms.
혼합 미생물이 식물 (Salvia)의 생장에 미치는 영향
최경민(Kyung-Min Choi),박응로(Eung-Roh Park),주흥신(Hong-Shin Ju),앙재경(Jae-Kyung Yang),서정근(Jeung-Keun Suh),이성택(Sung-Taik Lee),박창희(Chang-Hee Park) 유기성자원학회 1996 유기물자원화 Vol.4 No.2
광합성 세균, 젖산균, 효모 등의 미생물을 토양 관주, 엽연 시비의 방법으로 식불 (sa!νia) 에 처리하고 식물의 생장에 마치는 효과를 검토하였다. 액체 배양한 광합성 세균을 100 배 희석하여 토양 관주할 경우 다른 미생물군을 단독으로 처리한 경우에 비해 식물의 생장이 우수하여 대조구보다 약 160%의 생장 촉진 효과를 나타내었다. 미생물군을 혼합하여 처리한 경우에는 세 종류의 미생물을 혼합한 후 10 배 희석하여 처리한 경우에 가장 우수한 효과를 나타내었으며 대조구보다 212% 식물 생장이 촉진되었다. Effect of effective microorganisms on the growth of plant (salα:a sp.) was investigated. Microorganisms used were photosynthetic bacteria, lactic acid bacteria and yeasts. When photosynthetic bacteria were inoculated to soil by 100 dilution, treated plants showed 160% growth by length compared to control. When photosynthetic bacteria, lactic acid bacteria and yeasts were mixed, diluted by 10 and inoculated to soil, the plants showed 212% growth compared to control. Microbial populations were increased in the treated soil.