비소세포 폐암 환자의 이차 치료로서 Gemcitabine과 Vinorelbine 혼합 요법의 효과

장필순,강현모,이정은,권선중,안진영,이연선,정성수,김주옥,김선영 충남대학교 암공동연구소 2006 암공동연구소 업적집 Vol.5 No.

Background : Both gemcitabine and vinorelbine are effective anticancer drugs with mild toxicity on non-small cell lung cancer, and monotherapy of these drugs are effective as a second-line chemotherapy. The aim of this trial was to assess the response and toxicity of a combination of gemcitabine and vinorelbine in patients of previously treated for non-small cell lung cancer. Materials and Methods : 24 patients, initial stage III A/B,IV and previously treated with platinium and taxane based regimens, were enrolled from June 2000 to March 2004. The regimens consisted of vinorelbine 25mg/㎡ followed by an infusion of gemcitabine 1000mg/㎡ on day 1 and day 8 every three weeks. This course was repeated more than twice. Results : Twenty-four patients were analyzed for the response, survival rate, and toxicities. The overall response was 17% with a complete remission rate of 4%. The median time-to progression (TTP) was 3.1 months (95%, CI l-10months), and the survival time was 8.2 months (95%, CI 1-23 months). The grade 3/4 toxicities encountered were neutropenia (12.5%), anemia (0%), thrombocytopenia (0%). Non-hematological 3/4 toxicities were not observed. Conclusion : A combination of gemcitabine and vinorelbine in patients previously treated for non-small cell lung cancer provides a relatively good response rate, and a low toxicity profile. However, further study will be needed to confirm its effectiveness. (Tuberc Respir Dis 2005; 58: 344-351) 배경 : Gemcitabine, paditaxel, docetaxel, vinorelbine, ir-rinotecan 새로운 항종양제의 출현으로 일차 치료의효과가 증대 되고 있고 재발시에도 좋은 신체 활동도를 보이고 있어서 이차 치료의 대상군도 늘어나는 효과를 보이고 있다. 치료의 필요성은 증대 되고 있지만 현재까지 표준 치료가 확립되지 않은 상황에서 Gem-citabine과Vinorelbine모두 독성이 강하지 않아서 혼합 요법이 가능한 장점이 있고 비소세포 폐암에 대한효과도 입증이 되어있어서 본 연구는 반응이 없거나 반응을 보인후에 재발된 진행된 비소세포 폐암에 ge-mdtabine과 vinorelbine 흔합 요법을 시행하여서 치료 반응률과 생존율 그리고 부작용을 평가하였다. 대상 및 방법 :2000년 6월부터 2004년 3월까지 충남대학교병원에내원하여 진행성 비소세포 폐암 IIIA/IIIB, IV로 진단을 받고 일차 항암화학요법치료를 받은 환자중에 초기 치료에 반응이 없거나, 치료에 반응이 있었으나 병이 진행된 환자로 추적 관찰 기간이 6개월 이상인 환자를 대상으로 생존율과 반응률 그리고 독성을 분석하였다. 결과 : 총 치료 반응률은 17%. 반응 유지기간의 중앙값은 3.1개월(1-10개월)이었고 생존기간의 중앙값은 8.2개월 (1-23개월) 그리고 1년 생존율은 35%였다. 항암화학요법에 의한 독성은 3도 이상의 중성구 감소가 12%, 오심과 구토가 12.5% 였다. 결론 : 일차 치료에 반응이 없거나 재발한 비소세포 폐암환자의 이차 치료로 gemcitabine과 vinorelbine 혼합요법은 효과적이라고 생각되며 향후 3상 연구를 통한다른 약제와의 비교 연구가 필요하다고 생각된다.

비소세포 폐암 환자의 이차 치료로서 Gemcitabine과 Vinorelbine 혼합 요법의 효과

장필순,강현모,이정은,권선중,안진영,이연선,정성수,김주옥,김선영 충남대학교 암연구소 2006 암연구소 업적집 Vol.5 No.-

Background : Both gemcitabine and vinorelbine are effective anticancer drugs with mild toxicity on non-small cell lung cancer, and monotherapy of these drugs are effective as a second-line chemotherapy. The aim of this trial was to assess the response and toxicity of a combination of gemcitabine and vinorelbine in patients of previously treated for non-small cell lung cancer. Materials and Methods : 24 patients, initial stage III A/B,IV and previously treated with platinium and taxane based regimens, were enrolled from June 2000 to March 2004. The regimens consisted of vinorelbine 25mg/㎡ followed by an infusion of gemcitabine 1000mg/㎡ on day 1 and day 8 every three weeks. This course was repeated more than twice. Results : Twenty-four patients were analyzed for the response, survival rate, and toxicities. The overall response was 17% with a complete remission rate of 4%. The median time-to progression (TTP) was 3.1 months (95%, CI l-10months), and the survival time was 8.2 months (95%, CI 1-23 months). The grade 3/4 toxicities encountered were neutropenia (12.5%), anemia (0%), thrombocytopenia (0%). Non-hematological 3/4 toxicities were not observed. Conclusion : A combination of gemcitabine and vinorelbine in patients previously treated for non-small cell lung cancer provides a relatively good response rate, and a low toxicity profile. However, further study will be needed to confirm its effectiveness. (Tuberc Respir Dis 2005; 58: 344-351)

혈액 투석 환자에서 중심정맥 협착에 대한 스텐트 삽입술 : Wallstent Placement

임대승,노상필,이유선,정승현,김보영,이정우,강정아,김정희,이민수,정준용,최시완,정진옥,성인환,이강욱,신영태 충남대학교 의과대학 의학연구소 2002 충남의대잡지 Vol.29 No.1

Stenosis of central vein is a common complication arising after percutaneous subclavian vein catheter insertion performed for temporary vascular access in chronic renal failure patients undergoing hemodialysis. There are several treatment methods for the condition like percutaneous angioplasty(PTA), stent insertion, and surgery, but recent trend is toward PTA and stents. Among the patients diagnosed with chronic renal failure from March 1993 to May 2002 and undergoing hemodialysis through AV fistula, the 14 Patients in whom central vein stenosis arose were selected for the study. A total of 28 percutaneous interventions(5 PTA and 23 stent placement) were performed, and restenosis rate and the time taken till the restenosis in de novo lesions and instant lesions were compared. All 28 cases were operated successfully. The 14 cases that received both anigioplasty and stent placement initially. (de novo lesion : 14 cases), Among the 10 cases with de novo lesion that followed up more than 1 year, 3 cases are currently undergoing hemodialysis without restenosis, and the remaining 7 cases have recurred stenosis with the mean time to restenosis of 10.9 months. In the 7 cases in whom stenosis recurred, 11 interventions were done(instent lesion: 11 cases). 4 of these were using only ballon angioplasty with 100% restenosis rate and the mean time of 3 months until restenosis. The remaining 7 cases were using both balloon angioplasty and stent placement, also with 100% restenosis rate but with the mean time of 12 months until restenosis, which was later than the group receiving only balloon angioplasty. In treating the patients with central vein stenosis, stent placement seems to be more advantageous over PTA in terms of restenosis rate and the mean duration of patency. In the case of instent lesion, inserting the stent for the second time after stenosis recurred lengthened the duration of patency compared to performing balloon angioplasty alone.

P163 IL-17A producing ILC3s are increased in HDM-induced atopic dermatitis mice

( Seon-pil Jin ),( Min Ho Kim ),( Jungyoon Ohn ),( Jin Ho Chung ),( Dong Hun Lee ),( Hye Young Kim ),( Kyu Han Kim ) 대한피부과학회 2016 대한피부과학회 학술발표대회집 Vol.68 No.2

<div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div> Background: During past several years, it has become clear that innate lymphoid cells (ILCs) play a role in homeostasis and inflammation of the skin in humans and mice. The ILCs lack expression of antigen-specific receptors as those expressed by T and B cells and are activated by specific cytokines. In the skin, ILC are well placed to sense keratinocyte-derived danger signals in an antigen-independent manner. Recent findings llink ILC2 to atopic dermatitis and ILC3 to psoriasis. Objectives: The goals of this study were to investigate whether ILCs are involved in house dust mite (HDM) induced atopic dermatitis (AD) and to look for molecular mechanisms of activation of ILCs. Methods: We applied D. farinae extract to the barrier-disrupted skin of NC/Nga mice 2/week for 2 weeks.Skin samples were obtained to analyse ILCs and signals that stimulate ILCs.Flow cytometry, qPCR and histologic examination were performed. Results: The prevalence of ILCs in skin was greater in the AD induced mice than in the control. ILCs from skin with AD produced significantly larger amounts of IL-13 as well as IL-17A. Unexpectedly, IL-17A producing ILC3s were dominant than IL-13 producing ILC2s at the site of inflamed skin. Moreover, blockade of IL-17A mitigated the development of AD in Nc/Nga mice. Conclusion: IL-17A producing ILC3s are increased in HDM induced AD mice, suggesting potential role of ILC3s in the immune-pathogenesis of AD. Defining the IL-17-ILC3 axis in addition to ILC2 may represent a new target of AD.

Imiquimod-applied interleukin-10 deficient mice better reflects severe and persistent psoriasis with systemic inflammatory state

( Seon-pil Jin ),( Seong-joon Koh ),( Da-ae Yu ),( Min-woo Kim ),( Hee Tae Yun ),( Dong Hun Lee ),( Hyun-sun Yoon ),( Soyun Cho ),( Hyun-sun Park ) 대한피부과학회 2017 대한피부과학회 학술발표대회집 Vol.69 No.2

Background: Transgenic mice have been investigated for the imiquimod-induced psoriasis model. However, as compared to human psoriasis, the lack of chronicity in the previous models remains as a major limitation. Objectives: To evaluate the imiquimod-applied interleukin (IL)-10 knock-out (KO) mice better represent psoriasis than the previous models. Methods: Imiquimod or vehicle cream was applied to the ear and shaved back of the IL-10 KO mice and wild type (WT) mice, and each mice group was sacrificed on day 3 or day 15. We compared the inflammatory responses, such as clinical and histological features and cytokine profiles, using immunohistochemistry, quantitative reverse transcription-polymerase chain reaction, and enzyme-linked immunosorbent assay. Results: Imiquimod-induced skin inflammatory responses were observed in both IL-10 KO mice and WT mice on day 3. However, IL-10 KO mice exhibited significantly higher clinical and histopathological severity index than did WT on day 15. In cytokine profiles, IL-10 KO mice showed significantly higher IL-23p19 mRNA expressions and serum levels of IL-17A and tumor necrosis factor-α on day 15. In addition, IL-10 KO mice showed significantly higher spleen weight to body weight than did WT on day 3 and day 15. Conclusion: These results suggest that imiquimod-applied IL-10 KO mice might be a good model that can reflect chronic and severe psoriatic features.

P089 : Toll-like receptor 2 mediates a cutaneous reaction induced by repetitive ultraviolet b irradiation in c57/bl6 mice in vivo

( Jin Yong Kim ),( Jung Yoon Ohn ),( Hyun Sun Park ),( Seon Pil Jin ),( Youn Gae Lee ),( In Gyung Oh ),( Serah Lee ),( Jung Ho Kim ),( Kwang Hyun Cho ),( Jin Ho Chung ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2

Background: Toll like receptors (TLR) are a family of pattern recognition receptors that play an essential role in the induction of the immune response. Objectives: Previous studies suggested that single ultraviolet (UV) exposure may initiate a TLR-mediated sterile inflammation in vitro. This study was conducted to verify whether TLR2 mediates sterile inflammation, which is induced by repetitive to UVB exposure in vivo. Methods: Inflammatory responses to repetitive UVB irradiation were compared between wild-type (WT) and TLR2 knock-out (KO) mice. Results: After 6 weeks of repetitive UVB irradiation, inflammatory response of the TLR2 KO group was less severe than that of the TLR2 WT group. Compared to the group of TLR2 WT mice, the group of TLR2 KO mice displayed less prominent erythema and scaling; and histopathologically fewer inflammatory cells and significantly thinner skin. UVB-induced expression of the heat shock protein 70, an endogenous ligand of TLR2, was lower in the TLR2 KO group. Quantitative RT-PCR was used to measure the gene expression levels of interleukin (IL)-1β, IL-6, and matrix metalloproteinase (MMP)-13, all of which were lower in the TLR2 KO group, compared to the WT group. This finding was further corroborated with ELISA and western blot, each of which showed lower protein expression level of IL-1β, and MMP-13, respectively. Conclusion: This study demonstrated that TLR2 is involved in cutaneous inflammatory reaction to repetitive UV irradiation in C57/B16 mice.

A Rapidly Regressing Wart Following Biopsy

Seon-Pil Jin,Yoon Kyung Jeon,Kwang Hyun Cho,정진호 대한피부과학회 2011 Annals of Dermatology Vol.23 No.1

N-glycosylation in desmoglein is required for desmosome assembly

( Seon Pil Jin ),( Jang Hee Oh ),( Eun Young Seo ),( Jin Ho Chung ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2

Background: Desmogleins are considered to have an N-linked glycosylation. The function of N-glycosylation in desmogleins has only been investigated in terms of antigenicity. However, the effect of N-glycosylation onmediating cell adhesion, the major role of desmogleins in a physiologic state, is to be elucidated. Results: When tunicamycin was treated to the cultured keratinocyte, western blotting showed shifted band of desmogleins, which means the reduction in size and deglycosylation of the target protein. The quantity of desmogleins also decreased. And to see if the cell adhesion strength is reduced at this deglycosylated state, dissociation assay was performed. The number of cell fragments revealed that tunicamycin treatment dissociated cell sheets 2.6 fold more than control. Decreased amount of desmogleins cannot fully explicate the disruption of adhesion, seeing that the result of dissociation assay comparing the treatment of tunicamycin to that of salubrinal which attenuates protein translation. Immunocytofluorescence showed abberant feature of desmogleins, but seemed to appear normal trafficking to the cell membrane. And under the transmission electron microscope, the outer and inner dense plaque became thinner in tunicamycin treatment than control. Thestability of desmogleins also significantly decreased in tunicamycin treated cells. Conclusion: Taken together, inhibition of N-glycosylation disrupts assembly of desmsomes by producing abnormal desmoglein.

Urban particulate matter in air pollution penetrates into the barrier-disrupted skin and produces ROS-dependent cutaneous inflammatory response <i>in vivo</i>

Jin, Seon-Pil,Li, Zhenyu,Choi, Eun Kyung,Lee, Serah,Kim, Yoen Kyung,Seo, Eun Young,Chung, Jin Ho,Cho, Soyun Elsevier 2018 Journal of dermatological science Vol.91 No.2

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Particulate matter (PM) is an integral part of air pollution, which is a mixture of particles suspended in the air. Recently, it has been reported that PM is associated with increased risks of skin diseases, especially atopic dermatitis in children. However, it is unclear if PM directly goes into the skin and what mechanisms are involved in response to PM.</P> <P><B>Objective</B></P> <P>To see whether PM could penetrate into the barrier-disrupted skin, produce reactive oxygen species (ROS), and elicit an inflammatory response.</P> <P><B>Methods</B></P> <P>We collected PMs during a winter in Seoul and used cultured keratinocytes for <I>in vitro</I> study and tape-stripped BALB/c mice for <I>in vivo</I> study.</P> <P><B>Results</B></P> <P>Keratinocyte cytotoxicity increased in a dose-dependent manner by PM treatment. IL-8 and MMP-1 mRNA expression and protein levels were significantly increased compared to control by qPCR and ELISA, respectively. Cellular ROS production was increased by PM treatment, and antioxidant N-acetyl cysteine pretreatment prevented induction of inflammatory cytokines IL-8 and MMP-1. In PM-treated keratinocytes, electron-dense subcellular particles were observed by transmission electron microscopy. PM was observed inside hair follicles in both intact and barrier-disrupted skin <I>in vivo</I>. Additionally, intercellular penetration of PM was seen in the barrier-disrupted skin. Repeated PM application induced epidermal thickening and dermal inflammation with neutrophil infiltration. Finally, N-acetyl cysteine could ameliorate skin inflammation induced by PM application.</P> <P><B>Conclusion</B></P> <P>PM penetrates into the barrier-disrupted skin, causing inflammation, demonstrating detrimental effects in the skin.</P> <P><B>Highlights</B></P> <P> <UL> <LI> It is unclear if particulate matter (PM) directly goes into the skin. </LI> <LI> We provide visual images of PM penetrating into epidermis in the barrier-disrupted skin. </LI> <LI> Repeated PM application leads to cutaneous inflammation via ROS-dependent manner. </LI> <LI> It may have clinical implications especially for patients with deficient skin barrier including atopic dermatitis, diabetics. </LI> </UL> </P>

e-Poster : Topical photodynamic therapy with methyl-aminolevulinic acid for the treatment of actinic keratosis (초)

( Seon Pil Jin ),( Hyun Sun Park ),( Kwang Hyun Cho ) 대한피부과학회 2010 초록집 Vol.48 No.20