Analysis of KAP1 expression patterns and human endogenous retrovirus Env proteins in ovarian cancer

Kyung‑Yoon Jeon,Eun‑Ji Ko,Young Lim Oh,Hongbae Kim,Wan Kyu Eo,김아리,Han Gyu Sun,Meesun Ock,Ki Hyung Kim,Hee‑Jae Cha 한국유전학회 2020 Genes & Genomics Vol.42 No.10

Background Human endogenous retroviruses (HERVs) constitute around 8% of the human genome and have important roles in human health and disease, including cancers. Previous studies showed that HERV envelope (Env) proteins are highly expressed in cancer tissues and co-related with cancer progression. KAP1 has been reported to play a key role in regulating retrotransposons, including HERV-K, through epigenetic silencing. Objective The relationship between KAP-1 and HERV Envs expressions was analyzed only in tumor cell lines and has not yet been studied in cancer tissues. In this study, we analyzed the expression patterns and relationship between KAP1 and HERV Env proteins in ovarian cancer tissues. Method The expression patterns of KAP-1 and HERV Env proteins, including HERV-K and HERV-R, were analyzed in ovarian cancer tissue microarrays that contained 80 surgical specimens, including normal ovary and malignant ovarian cancers. Results The expression of HERV-R Env and KAP1 proteins is signifcantly higher in ovarian cancer compared with normal ovary tissues. However, the expression of HERV-K Env did not change signifcantly in cancer tissues. The expression patterns of HERV-K Env and HERV-R Env signifcantly increased in early stages of cancer and KAP1 expression was higher in certain stage and types of cancers. However, the expression of HERV-K Env, HERV-R Env, and KAP1 did not change in diferent age groups. The correlation between the expression of KAP1 and HERV-Env, including HERV-K and HERV-R, was not signifcantly correlated. Conclusions The results of this study showed that there was no signifcant correlation between the expression of KAP1 and HERV Env proteins in ovarian cancer tissues, unlike studies with cell lines in vitro. These results suggest that the actual expression of HERV Env proteins in ovarian cancer tissues may be regulated through various complex factors as well as KAP1.

쇄목펄프폐수를 이용한 Bacillus thuringiensis var. israelensis 의 Spore-Crystal 복합체 생산에 관하여

윤경하,김영돈,김태전 서울保健大學 1991 論文集 Vol.11 No.1

Streptomyes strain에 의한 Polymeric 염료와 Azo염료들의 탈색에 관한 연구

김태전,윤경하,최한영 서울보건대학 1998 서울보건대학 부설 한국보건과학연구소 논문집 Vol.5 No.1

Two Actinomycetes strains that it was excellent for degradablitiy of lignocellulose and lignin, and the highest for decolorization ratio of Remazol brilliant blue R(RBBR) dye, which was good relationship with degradabiliy of lignocellulose and lignin were identified finally with Streptomyces halstedii scabies SAl-36과 Streptomyces lavendulas SA2-14. Authors used these two strains to examine into the visiable spectrum and decolorization ratios of Azo dyes : amaranth, metanil yellow, orangeII. tropaeolin O, congo red, and Polymeric dye; poly R-478. The results to be examined were summarized as following. ① The visible spectrum of poly R-478, and amaranth, congo red. metanil yellow, orange II, tropaelin O were indicated that poly R-478 was 520/350nm. and amaranth. 522/324nm ; congo red. 490/434nm ; metanil yellow. 436/380nm ; orange II 484/310nm ; Tropaeolin O, 410/324 nm ② Each decolorization ratios of Azo dyes and Polymeric dye by Streptomyces halstedii scabies SA1-36 were showed that tropaeolin O was 79.3% ; poly R-478, 78.8% ; amaranth, 75.4%; orange II, 67.0% ; metanil yellow, 51.4% ; and congo red, 47.5%, so that the decolorization ratios of tropaelin O was the highest, and congo red, lowest. Consequently Streptomyces halstedii scabies SA1-36 was regarded as a good strain for the decolorization of tropaelin O into Azo dyes and poly R-478. ③ Each decolorization ratios of Azo dyes and Polymeric dye by Streptomyces lavendulas SA2-14 were showed that tropaeolin O was 81.4%; poly R-478, 80.0%; orangeII, 74.1%; congo red, 65.0%; metanil yellow, 57.7%, so that the decolorization ratios of tropaelin O was the highest. and secondly poly R-478. higher. and other dyes indicated the decolorization ratios of 57.7%-74.1%. Consequently Streptomyces halstedii scabies SA1-36 was regarded as a good strain than Streptomyces halstedii scabies SA1-36, and a good strain for the decolorization of tropaelin O into Azo dyes and poly R-478. ④ Each decolorization ratios of Azo dyes and Polymeric dye by Streptomyces badius ATCC 39117 (control group) were showed that tropaeolin O was 80.3% ; poly R-478, 79.0% ; amaranth, 70.7%; orange II, 66.4% ; congo red, 57.4% ; methenil yellow, 50.3%, so that the decolorization ratios by control group was the highest in tropaelin O, and secondly higher in poly R-478 as well as two experimental strains. ⑤ Two experimental strains were decolorized all of the examined dyes at the early days of culture, but control group, at the latter days of culture. In the above results, two experimental strains that were identified as Streptomyces halstedii scabies SAl-36과 Streptomyces lavendulas SA2-14 was regarded as a good strains for the decolorization of tropaelin O into Azo dyes and Poly R-478, and indicated 1-8% of high decolorization ratios than control group in all of Azo dyes and poly R-478, and among three strains Streptomyces lavendulas SA2-14 was thought as the best strain, when it were based on the days of culture, two experimental strains were decolorized all of the examined dyes at the early days of culture, but control group. at the latter days of culture. In the future, it will be necessary to invastigate what kind of dyes series with the object of more dyes which are decolorized more effectively by two experimental strains, also it will be more important subjects to examine into enzymes produced by Actinomycetes and establish exactly mechanism of decolorization by various produced enzymes.

토양에서 분리된 방선균들에 의한 Rimazol Brilliant Blue R(RBBR) 염료의 탈색에 질소원과 pH가 미치는 영향

김태전,김승곤,윤경하 서울保健大學 1997 論文集 Vol.17 No.1

Many workers had reported that the degradation of lignocellulose and lignin by white rot fungi were significantily correlative with the degradation of phenol or PAHs (Polycyclic aromatic hydorcarbons) compounds and the decolorization of a various dyes, specially RBBR (Remazol brilliant blue R) and Poly R-478 dyes. but it were not known distinctly that the degradation of these and the decolorization of these by Actinomycetes were related to the degradation of phenol or PAHs compounds, and author had examined in relationship between the degradation of lignocellulose or lignin and the decolorization of RBBR by Actinomycetes isolated in 67 soil samples collected at 8 areas of Korea from February, 1994 to March, 1995. In the experiment, There were selected two strains identified as Streptomyces halstedii scabies SA1-36 and Streptomyces lavendulas SA2-14 which had good correlation between the degradation of lignocellulose or lignin, and it were investigated that the cultural parameters to maximize the decolorization ratios of RBBR by two strains, as the results, Streptomyces halstedii scabies SA1-36 revealed each the highest decolorization ratios with 73.4% in 0.30% glycerin and Streptomyces lavendulas SA2-14, with 74.1% in 0.25% cellobiose at 30℃ in 20 days of culture. There were used their results as basal data in this study that examined the effects of nitrogen sources and pH to maximize the decolorization ratios of RBBR. It were summarized as following that the results examined the effects of nitrogen sources and pH to maximize the decolorization ratios of RBBR by two strains. 1) It were the highest each as 75.7% in casein and 75.7% in yeast extract that the dicolorization ratios of RBBR by Streptomyces halstedii scabies SA1-36 and Streptomyces lavendulas SA2-14 strains according to nitrogen sources(0.l%). 2) It were the highest each as 76.8% in 0.1% casein and 78.3% in yeast extract that the decolorization ratios of RBBR by Streptomyces halstedii scabies SA1-36 and Streptomyces lavendulas SA2-14 strains according to the concentrations of casein and yeast extract. 3) It were the highest each as 76.8% in pH 7.6 and 79.4% in pH 6.6 that the decolorization ratios of RBBR according to pH by Streptomyces halstedii scabies SA1-36 and Streptomyces lavendulas SA2-14 strains. In the above results, we could knowe that it were each different as well as carbon sources that nitrogen sources and pH required between Streptomyces halstedii scabies SAl-36 and Streptomyces lavendulas SA2- 14 strains. and organic nitrogens influence more than inorganic nitrogens to decolorize RBBR dye and nitrogen sources and pH influence lower than carbon sources to decolorize RBBR dye. What`s more, we could knowe that it were very important to use newly formuliged optimal basal media maximize decolorization ratios of RBBR dye by Streptomyces halstedii scabies SAl-36 and Streptomyces lavendulas SA2-14 strains.

구속신장시험에 의한 토목섬유의 인장력-변형률거동 특성

방윤경,이준대,전영근 한국산업안전학회 2002 한국안전학회지 Vol.17 No.2

In this study, stress-strain relationships were investigated by performing the confined extension tests for seven types of geosynthetics such as geotextiles, composite geosynthetics and geogrids. A comparison was made between unconfined and confined moduli for each geosynthetic material to quantify the soil confinement effect on stress-strain properties. A comparison was also made between the increase of moduli at the same strain level with the types of the geosynthetics to demonstrate the different stress-strain responses. Based on the result of the extension tests, the higher the confining stress, the larger the secant modulus of geosynthetics. The secant modulus at 5% strain is twice as much as that of 10% strain, especially there is a noticeable increasing of secant modulus for the two nonwoven geotextiles.

리그닌을 분해하는 Streptomyces lavendulas SA-14에 의한 염료의 탈색

김태전,윤경하 순천향대학교 기초과학연구소 1998 순천향자연과학연구 논문집 Vol.4 No.1

This study was undertaken to investigate the activity of ligninolytic enzymes and the decolorization capability of some dyes with streptomyces lavendulas SA-14 isolated from soil and identified. Most ligninolytic enzymes exhibited the maximal activity when the strain was grown in basal medium for 6 days. Activities of LiP 2 and VAO among these enzymes exhibited 14.25 and 11.23n㏖/㎎ protein respectively. Decolorization rate of amaranth, orange Ⅱ, tropaeolin O and poly R-478 was more than 72% on 10 days incubation while that of crystal violet and malachite green was 6.7% and 6.6% respectively.

대학생의 상담에 대한 태도

김범선,전윤경,전진실 西江大學校 學生生活相談硏究所 2008 人間理解 Vol.29 No.-

본 연구는 성별, 연령 등의 인구학적 변인과 이전 상담 경험여부가 상담에 대한 태도에 어떠한 영향을 미치는 가를 알아보기 위해 실시되었다. 이를 위해 서울시내 S 대학에 재학 중인 528명의 학생들에게 상담에 대한 태도와 이전 상담 경험, 그리고 인구학적 특성들을 알아보는 설문지에 응답하게 한 후 그 결과를 통계적으로 분석하였다. 상담에 대한 태도는 Fisher 와 Turner(1975)의 상담태도에 관한 질문지로 측정되었다. 그 결과, 인구학적 변인 중 성별, 학년, 소속 학과, 재수 여부, 종교, 그리고 이전 상담 경험이 상담에 대한 태도에 영향을 미치는 것으로 나타났다. 즉, 여성이 남성에 비해, 천주교 집단이 다른 종교 집단에 비해 전문가에 대한 신뢰와 상담 욕구 면에서 유의미하게 높게 나타났고, 4학년 집단이 1학년에 비해, 문학부가 다른 학부에 비해 전문가에 대한 신뢰의 측면에서 유의미하게 높은 점수를 보였다. 재수를 하지 않은 집단이 삼수이상 집단에 비해 오점수용에서 더 유연한 것으로 나타났으며, 이전에 상담을 해 본 사람일수록 상담에 대한 태도도 더 긍정적이고, 상담전문가를 더 신뢰하며, 오점 수용에 유연하고, 상담 욕구도 높은 것을 알 수 있었다. 마지막으로 이를 통해 얻을 수 있는 논의점과 본 연구의 제한점, 후속 연구를 위한 제안점을 기술하였다. This study investigated college students' attitude toward counseling according to gender, age, and other demographic variables. Research participants were 528 S college students in Seoul. They filled out the Attitude Toward Seeking Professional Help Scale(Fisher & Turner, 1975) and demographic guestionnaire. To analyze data, correlation analysis and ANOVA were conducted. As results, gender, grade, major failure in previous college entrance exam, religion and previous counseling experience had significant influence on attitude toward counseling. Results indicated that women and catholic groups showed more positive attitude in Confidence and Need of seeking attitude toward counseling than other groups. The college seniors and the departs of literature groups showed more positive tendency in Confidence of seeking attitude toward counseling than others. Students who had not repeated a college entrance exam had more flexible attitude in Stigma. Students with previous counseling experience showed more positive tendency in Need, Stigma, Confidence and the whole attitude toward counseling. Discussions and limitations on this study were described.

서울의 PPNG 발생 빈도(1991-1992)

김재홍,윤기범,박평원,김영진,전경민,김영태,김중환,곽호,구상완,송민석,유옥,지혜구,김동원,문상은,박영립,정승호,성범진,성순제,엄주용,황정열,이기홍,이주협,전태진 대한화학요법학회 1994 대한화학요법학회지 Vol.12 No.1

The prevalence of PPNG among pretreated gonorrhea cases isolated at the STD clinic of Choong-Ku Public Health Center in Seoul has been studied and reported annually since 1981. In 1991, 123 strains of N.gonorrhoeae were isolated, among which 58(47.1%) were PPNG. In 1992, 98 starains of N.gonorrhoeae were isolated, among which 51(52.0%) were PPNG. In all, 109(49.3%) strains were found to be PPNG among 221 strains isolated between 1991-1992. The prevalence of PPNG in Seoul showed increased tendency till 1989, thereafter, it has been stationary or slightly decreasing.

동국대학경주병원에서 분리된 각종 병원성 세균 및 항생제 감수성 양상

하경임,고은하,전창호,정병욱,안우섭,김우택,배정수,어경윤 동국대학교 경주대학 1993 東國論集 Vol.12 No.-

We investigated characteristics of bacterial infection and antimicrobial suscerptibility patterns of clinical specimens in Dong Guk University Kyong Ju Hospital from october 1991 to december 1992. The types of clinical specimens requested for culture were as follows ; pus(34.2%), urine(29.6%), sputum(25.2%), blood(9.2%), etc. The most frequently isolated organisms were E. coli(17.6%), Pseudomonas(12.7%), S. aureus(12.2%), and CNS(11.2%), orderly. Predominant organisms from pus, urine and sputum were S.aureus, E. coli and Pseudomonas. Blood culture was requested 1,244 samples from 614 patients, 91 pathogeni organisms were isolated(14.8% from patient and 7.3% from requested clinical samples). E. coli was most frequently isolated(29.7%) and Salmonella typhi was noted 8.8% from the requested samples. The patterns of isolated organisms according to departments were as follows ; E.coli and Klebsiella were frequently isolated in internal medicine and general surgery, E.coli and Pseudomas in Neurosurgery, S. aureus and Pseudomonas in orthopedic surgery. In ICU, Pseudomonas was predominatly isolated and the rate of mixed infection was also high(16.4%). Most of gram-negative bacilli revealed relatively high susceptibility to Chlorampenicol, Amikacin, Tobramycin and Carbenicillin, but Acinetobacter were only susceptible to Carbenicillin and Tetrecyclin, and Serratia were to Carbenicillin and Amikacin while Salmonella were susceptible to all tested first drugs. The proportions of multi-drug resistant bacteria were 34.9% of Acinetobacter, 21.1% of Serratia, 20.0% of Pseudomonas and 15.4% of Citrobacter but Aztreonam was highly susceptible(84∼100%) except Acinetobacter. S. aureus and CNS were susceptible to Chlorampenicol, Oxacillin, Cephalothin and Vancomycin, especially, 100% susceptible to Vancomycin. But Methicillin reistant Staphylococcus aureus were very high(49%) inspite of new constructed hospital. Pneumococcus and Streptococcus species were relatively high susceptible to all tested drugs.

쇄목펄프폐수를 이용한 Bacillus thuringiensis var. israelensis의 Spore-Crystal 복합체 생산에 관하여

김영돈,김태전,윤경하 순천향대학교 1991 논문집 Vol.14 No.3

쇄목펄프공정폐수를 이용하여 상품균주인 Bacillus thuringiensis var. israelensis (Comm. B.t.i)와 serotype H-14인 Bacillus thuringiensis var. israelensis(Serotype B.t.i)의 spore-crystal복합체 생산성과 폐수의 BOD 감소량을 조사하였다. 펄프폐수에 Peptone 1.0%, K₂HPO₄ 0.05%, MgSO₄ㆍ7H₂O 0.01% 및 CaCl₂0.02% 등을 첨가하여 균주를 30℃에서 200rpm 으로 진탕배양했을때 Comm. B.t.i는 배양40시간에, Serotype B.t.i는 배양 32시간에 최대 생장을 나타냈다. 배양 72시간에 생성된 아포 수는 Comm. B.t.i는 8.3 ×?? spores/ml, Serotype B.t.i는 6.4 x ?? spores/ml였고, 폐수의 BOD는 71-72%의 감소를 나타냈다. 두 균주가 생성한 spore-crystal복합체의 농도가 ?? spores/ml 일때 Culex pipens 유충은 12시간내에 100% 치사되었다. Effect of some nutrient on the growth of Bacillus thuringiensis var.israelensis in the ground wood pulp will waste liquor was determined during an attempt to lower the BOD content of the waste liquor and to produce spore-crystal complex by bacteria. The stain applied were commercial Bacillus thuringiensis var. israelensis (Comm. B.t.i) and serotype H-14, Bacillus thuringiensis var. israelensis(Serotype B.t.i), The necessity of the addition of 1.0% pep-tone, 0.05% K₂HPO₄, 0.01% MgSO₄7H₂O and 0.02% CaCl₂for the best growth of two strains in the waste liquor was ascertained as a result. Comm. B.t.i was at a maximal growth after the 40 hour of incubation but Serotype B.t.i, after the 32 hour of incubation of the strains in the supplemental waste liquor, spore count of Comm. B.t.i and Serotype B.t.i were 8.3 x ?? spores/ml and 6.4 x ?? spores/ml respectively and the BOD content of the waste liquor was lowered by about 70%. Two strains showed 100% of lethal rate, when Culex pipiens larvae were exposed to ?? spores/ml of concentration of spore-crystal complex.