Nd:YAG 레이저 조사와 불화물 도포가 탈회법랑질의 재광화에 미치는 영향

조현,이광희,김대업 大韓小兒齒科學會 2004 大韓小兒齒科學會誌 Vol.31 No.3

Normal pulsed Nd:YAG 레이저 조사와 불소화합물이 산성음료수에 의해 탈회된 법랑질의 표면 미세경도에 미치는 재광화 효과를 평가하고자 한다. 법랑질 시편을 제작하여 50ml 플라스틱 시험관 5개에 코카콜라를 채운 후 30개의 소구치를 6개씩 나누어 넣고 탈회시켰다. 법랑질 표면에 6W 출혁하의 50mJ 에너지 밀도, 20Hz의 pulse rate로 레이저를 조사하였다. 레이저가 조사된 법랑질 표면에 각 군별로 여러 형태의 불소화합물을 처리한 후 탈회 전, 후의 미세경도 및 Diagnodent를 측정하였다. 동일한 방법으로 탈회된 법랑질에 레이저를 조사했을 때와 레이저가 조사된 법랑질 표면에 불소화합물을 처치한 후에 미세 경도 및 Diagnodent를 측정하였다. 법랑질 표면을 산성 음료에 탈회 후, 레이저 조사 후, 불소화합물 처치 후의 각각의 시편에 대하여, 주사전자현미경으로 1500배 확대하여 관찰하였다. 1. 산성 탈회된 법랑질 표면의 미세경도(VHN)가 레이저를 조사한 수와 불소 도포 후에 증가하였으나, 레이저 조사 후의 경도와 불소 도포 후의 경도간의 차이는 유의하지 않았다. 2. 초기 경도, 탈회 후 경도, 레이저 조사 후 경도, 불소 도포 후 경도에서 불소군 간의 차이는 모두 유의하지 않았고, 불소 용액 군과 불소 젤 군에서는 레이저 조사 후의 경도와 불소 도포 후의 경도 간에 유의한 차이가 없었으나, 불소 바니쉬군에서는 유의한 차이가 있었다. 3. Diagnodent 측정치의 변화에서 초기 점수, 탈회 후 점수, 레이저 조사 후의 점수와 불소 도포 후의 점수 간에는 유의한 차이가 있었고, 레이저 조사 후 점수와 불소 도포 후의 점수가 불소 도포 군 사이에서는 유의한 차이가 없었다. 4. 산성 음료수에 탈회된 법랑질 표면에의 Nd:YAG 레이저 조사는 재광화에 효과적이며, 불소화합물의 처치는 레이저의 효과를 증가시키는 부가적인 효과를 갖는다. 5. 주사 전자 현미경상에서 Nd:YAG 레이저가 조사된 법랑질 표면은 전체 표면이 법랑소주가 녹아내려 융합된 매끈한 바닥에 균열이 생기고 분화구가 퍼져 있는 양상을 보였으며, 불소 도포 후에는 균열과 분화구 형태는 뚜렷하지 않으며 탈회된 후의 표면에 가까운 양상을 보였다. The purpose of this in vitro study was to investigate the effects of Nd:YAG laser irradiation and fluorides on acid demineralized enamel. The materials were 30 freshly extracted permanent premolars with intact smooth enamel surfaces. They were demineralized with Coca-cola at 37℃ for 12 hours and then irradiated by Nd:YAG laser with 6W power, 50mJ/㎠ energy density, and 20Hz pulse repetition. After laser irradiation, teeth were treated by three kinds of fluorides; (1) 0.05% NaF fluoride solution (2) 1.23% APF gel and (3) 0.1%F fluoride varnish, microhardness(VHN) and Diagnodent scores were measured and the surfaces of each treated specimens were also observed with SEM under 1500 magnification. The results were as follows: 1. In the change of microhardness(VHN), it decreased to 34.68% from the initial micrhardness, increased to 78.37% after laser irradiation and increased to 82.62% after fluoride treatment, there were significant differences except when it was irradiated and treated with fluoride(P<0.05). 2. In the change of Diagnodent scores, it was decreased to 28.08% from the initial scores after demineralization, and then increased to 59.81% after laser irradiation, and increased to 82.17% after fluoride treatment. Scores were different significantly between the scores of initial, demineralization, laser irradiation and fluoride treatment (P<0.05). All the scores were not different significantly between fluoride types. 3. SEM observation showed that the lased enamel surfaces after demineralization were thermally degenerated and showed molten lava-like appearance and crater with cracks and many microholes.

만성 치열 환자에 대한 GTN 국소요법과 보존적 치료의 효과 비교

이광렬,조경아,황도연,김건욱,박원갑,윤서구,김현식,이종균 대한대장항문학회 2000 Annals of Coloproctolgy Vol.16 No.6

심실중격결손증-치험 1예-

조광현,우종수,황윤호,이양행,박철호,김종성,김철호,이경순,조영일,박태인 인제대학교 1985 仁濟醫學 Vol.6 No.4

A successful treatment of infants and children with congenital heart defects depends upon several factors: diagnosis must be accurate, the operative technique, including techniques of perfusion and myocardial preservation, must be suited to the needs of patients and intensive cares, including accurate anesthesia, must be applied through all stages of investigation and treatment, i.e. before, during and after the operation. Recently we performed a open heart surgery successfully for a six-years old girl who had suffered from frequent upper respiratory tract infection and mild to moderate exertional dyspnea with congenital heart defects (ventricular septal defect, Kirklin type II, with patent foramen ovale). The operation and postoperative course were very smooth. And now we report this with review of literatures.

황토 급여가 거세한우의 발육에 미치는 영향

조원모,기광석,정하연,강수원,김준식,백봉현,김용국 한국국제농업개발학회 2003 韓國國際農業開發學會誌 Vol.15 No.1

본 연구는 거세한우 육성우에 대한 황토 첨가급여가 발육에 미치는 효과를 구명하고자 발육능력, 사료섭취량을 규명한 바 아래와 같은 결과를 얻었다. 전 시험기간 동안의 평균 일당증체량은 대조구와 황토급여구가 각각 0.91㎏과 0.87㎏/day로 대조구가 높은 것으로 나타났지만 유의성은 인정되지 않았다. 사료섭취량은 대조구와 황토급여구 모두 평균 일일 두당 농후사료 섭취량이 2.42㎏이었으나, 건초섭취량에 있어서는 각각 3.02와 3.14㎏으로 황토급여구가 다소 높은 경향으로 나타났다. The study was conducted to investigate the effect of dietary supplements of reddish clay on the growth performance in growing Hanwoo steers. A total of 8 calves was allocated into 2 feeding groups, control(basal diet) and treatment(basal diet plus ad libitim Reddish clay) for Hanwoo steers. This study was carried out for 5 months with a total of 8 calves. The results obtained in the present studies are summarized as follows : Average daily gains throughout the experimental period of 4 months to 9 months of age were 0.91 and 0.87㎏ for control group and reddish clay-supplemented group, respectively, however, no statistical significances were found. Average daily intake of concentrates was 2.42㎏ for each group, while that of hay tended to be higher in reddish clay-supplemented group(3.14㎏) than in control group(3.02㎏).

유사체액에서의 전기화학적 처리가 티타늄 표면의 인산갈슘 흡착에 미치는 영향

조국현,김문영,송광엽,배태성,이민호 大韓齒科器材學會 2002 대한치과재료학회지 Vol.29 No.3

This study was performed to examine the effect of electrochemical treatment on the precipitation of apatite-like calcium phosphate in a simulated body fluid. Titanium plates of 20×20×1 ㎣ in dimensions were polished sequentially from #600 to #2,000 emery paper and one surface of each specimen was received an additional polishing sequence through 0.1㎛ alumina paste. To improve the bioactivity of titanium plates, they were electrochemically treated at 100 mA for 20 min in an electrolyte containing the calcium and phosphate ions, and the Hanks' solution with pH 7.4 at 36.5℃ for 30 days, and the surfaces were examined with X-ray diffractometer(XRD), scanning electron microscope(SEM) and X-ray photoemission spectroscopy(XPS). Precipitation of calcium phosphate and CaO were observed on the electrochemically treated titanium surface in a simulated body fluid after treatment at 600℃. Precipitation of calcium phosphate was densified and coarsened with increasing the temperature of the electrolyte solution in the range of 20-80℃. The calcium phosphate contained phosphate in the form of Po₄^3-, HPO₄^2-, and H₂PO₄^-.

호스피스 시설의 유형별 공간구성에 관한 건축 계획적 연구

조광현,박재승 한국의료복지시설학회 2002 의료·복지 건축 Vol.8 No.1

Recently cancer, AIDS, chronic sickness have increased according to the elevation of socioeconomic level and fast change of lifestyle. The number of patients receiving terminal care increased fairly because the span of life is extended by development of medicinal technology. Also necessity of hospice and palliative care was risen according to the request of terminal patients that remove pain and keep calm life by interest about quality of life, However architectural plan and type specialization of facility which can correspond team's composition and supplied nursing program are not consisting. This study researches about care environment of hospice facility plan through investigation into terminal patient's special quality. The purpose of this study is to propose fundamental datas of hospice facility for architectural plan through comparative analysis of cases of domestic and outside facilities.

심장수술 2,450예와 심장이식

조광현,황윤호,이양행,류지윤 인제대학교 1999 仁濟醫學 Vol.20 No.1

瓦松의 알콜 抽出物이 心筋 및 腸筋收縮에 미치는 影響

曺石鉉,金光鎭 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.1

Orostachys malacophyllus F. is a kind of ,Chinese medicine to grow on the tiled roof of the house or on the rock of the mountain. The chemical compositions and actions have not been reported, but, sometimes, it was used as a folk remedy for the treatment of cancerous diseases. This study was attempted to investigate the effects of water fraction separated from alcohol extract of Orostachys malacophyllus F. on the contractilities of cardiac and intestinal muscles comparing with those of the water extract. The muscles used for this study were the ventricular muscle of turtle and the intestinal muscle of mouse, and their contractilities were represented as a change of the muscle tension detected by myograph. The results were summarized as follows: 1. The water fraction decreased the contractility of ventricular muscle, and increased the intestinal contractility. But the water extract was observed the opposite effects on the contractility of each muscle. 2. Sympathetic or parasympathetic blocker and alpha-or beta-blocker had no effect on the decreased or increased contractility of each muscle. 3. Calcium antagonist(verapamil) inhibited significantly the contractility in the case of the water fraction to the intestinal muscle and the water extract to the ventricular muscle. From the above results, it was suggested that the action of water fraction or the water extact affected to calcium transport: release into the sarcoplasmic fluid or active influx into the sarcoplasmic reticulum in the case of the cardiac muscle, and release from ECF or cisternae of reticulum into the muscle cell in the smooth muscle.

MRI용 초전도 능동차폐 마그네트의 설계에 관한 연구

조윤현,이광호,김성도 東亞大學校 附設 情報通信硏究所 1996 情報通信硏究所論文誌 Vol.4 No.1

An optimal design approach is presented for the high homegeneity superconducting magnet with a superconducting active shield esperically for a magnetic resonance imaging system. This paper is investigated magnetic phenomena for a stray magnetic to get reduction technigues of the unwanted stray magnetic field from the superconducting magnet.

원예작물의 염류장애 원인과 대책을 위한 생리, 생화학적 연구 2. 토마토 뿌리조직 H+ 펌프 활성 및 Thapsigargin 저해효과

조광현,사공정,김영기 충북대학교 한국과학재단 지정 첨단원예기술개발 연구센터 1997 연구보고서 Vol.2 No.-

Thapsigargin은 동물조직 SR/ER-type Ca²+-ATPase에 특이성이 높은 선택적 저해제로서 토마토 뿌리조직으로부터 분리한 마이크로솜에 Ca²+-ATPase의 존재를 확인하기 위하여 사용되었다. 원형질막과 액포막에 위치하는 H+-ATPase들의 활성은 각각의 선택적 저해제인 vanadate와 NO₃를 이용하여 평가하였고, 이들의 활성은 각각 마이크로솜 ATPase 총활성의 ∼26%, ∼36%로 나타났다. 이들 두 가지 저해제 효과는 additive하게 나타났으며, 전체활성의 약 50∼70%를 저해함을 확인하였다. 마이크로솜 ATPase활성에 대한 thapsigargin의 저해효과에서 10 μM thapsigargin은 총활성의 30%를 저해하였으며, 저해효과는 농도 의존적으로 나타났다. Thapsigargin에 의한 활성저해 기작을 조사하기 위하여 H+-ATPase의 선택적 저해제들을 사용하였다. 액포막의 H+-ATPase 활성에 대한 thapsigargin의 효과를 조사하기 위한 NO₃의 dose-response 실험에서, thapsigargin은 NO₃에 의해 저해되는 ATPase활성을 경쟁적으로 감소시켰다. 반면, vanadate의 농도를 10 nM∼2mM로 증가시켜 원형질막의 H+-ATPase 활성을 저해시킨 조건에서는 thapsigargin에 의하여 저해되는 활성이 온전히 나타나 vanadate의 저해효과와는 무관한 결과를 얻었다. 이러한 결과는 액포막 H+-ATPase의 활성이 thapsigargin에 의해 저해된다는 것을 의미한다. 저자들은 액포막 H+-ATPase의 활성이 Ca²+에 의하여 저해됨을 이미 보고하였다 (Cho et al., 1998). Thapsigargin에 의하여 저해되는 마이크로솜 ATPase활성은 반응용액의 Ca²+농도가 증가할수록 감소하였으며, 이것은 thapsigargin이 액포막 H+-ATPase 활성을 저해한다는 가능성을 더욱 지지하였다. 이상의 결과는 토마토 뿌리조직의 마이크로솜 ATPase중 액포막의 H+-ATPase 활성이 thapsigargin에 의해서 저해되는 것을 보여주며, 액포막 H+-ATPase와 ER-type Ca²+-ATPase와의 구조적, 기능적 연관성이 있을 가능성을 제안한다. The effect of thapsigargin, a SR/ER-type Ca²+-ATPase inhibitor, was investigated to determine the presence of thapsigargin-sensitive Ca²+-ATPases in the microsomes of tomato roots. The activities of plasma membrane H+-APTase and vacuolar H+-ATPase were evaluated to ∼26% and ∼36% of total microsomal ATPase activity by using their specific inhibitors, vanadate and NO₃, respectively. The inhibitory effects of vanadate and NO₃- were additive and the simultaneous additions of these two inhibitors decreased the total activity up to 50∼70%. Thapsigargin (10μM) inhibited the microsomal ATPase activity by ∼30% and the thapsigargin-induced inhibition was appeared to be dose-dependent. In order to understand the molecular mechanism of thapsigargin-induced inhibition, the effects of specific H+-ATPase inhibitors were tested and compared to the effect of thapsigargin. The NO₃-induced inhibition was decreased by simultaneous treatment of thapsigargin. In dose-response experiments of vanadate and NO₃-, thapsigargin only blocked the NO₃-induced inhibition, while the effects of vanadate and thapsigargin were independent at the whole range of vanadate concentration (10 nM∼2 mM). Meanwhile, the thapsigargin-induced inhibition was decreased by increasing Ca²+ concentration. Since we have shown that Ca²+ blocks the activity of vacuolar H+-ATPase, these results together suggest that thapsigargin inhibits the NO₃-sensitive vacuolar H+-ATPase but not the vanadate-sensitive plasma membrane H+-ATPase. Our results also suggest that there is structural and functional relations between vacuolar H+-ATPase and SR/ER-type Ca²+-ATPase.