관상 동맥 질환에서 아포 E 지단백 유전자 다형성과 혈청 지질치와의 관계

곽선영,김성구,정호석,이유경,이광희,김철현,최태명,현민수,권영주 순천향의학연구소;Soonchunhyang Medical Research Institute 2000 Journal of Soonchunhyang Medical Science Vol.6 No.1

Background and aims: The Apolipoprotein E is a ligand of both the protein component LDL receptor as well as the apo E LDL-Receptor related protein (LRP). It modulates the receptor binding of lipoproteins, with the apolipoprotein E found on cell surfaces as its component, thus serving an important role in the lipid metabolism by carrying out the intracellular transport of cholesterol in lipoproteins. The gene for apolipoprotein E is the product of three common genotypes as well as many more rare alleles. The common genotypes are ε2, ε3, and ε4, and are expressed in the three phenotype isoforms of E2, E3, and E4. In the event that E4 is the main component, a rise in the cholesterol level, as the result of down-regulation of the LDL receptor, is observed. Therefore, those samples with E4 genotypes are known to be in much higher risk of coronary artery disease than those with ε3/ε3, while those with ε2 are in low risk (with the exception of hypertiglyceremai Ⅲ). The aim of this study is to analyze in patients with ischemic heart disease the role of aplipoprotein E alleles in order to seek its correlation with coronary artery disease, as well as to seek whether the polymorphism of apo E produces any differences in the severity of coronary artery disease according to plasma lipid levels. Methods: The subjects for study were 273 patients admitted to the Internal Cardiology Division of the Soonchunhyang University Hospital form December 1998 to February 1999. The subjects were divided into the two groups of which one was ischemic heart disease (IHD) experiment group totaling 105 (avg.60.1 years of age, male/female ratio = 69/36) and the control group totaling 168 (avg. 59.7 years of age, male/female = 73:95). The coronary angiogram was given to 127 subjects, and of this total, 94 have developed significant stenosis in the coronary artery. The stages of the analyzing of the apo E phenotype was first, the separation of DNA from the blood samples, subjecting it to the PCR from with 228 base pairs of expanded products were obtained. The band was determined by means of the reverse hybridization principle on the nitrocellulose strip. Results: From the 105 patients the distributions of apo E phenotypes were as follows: ε3/2(5.7%), ε4/2(1.9%), ε3/3(70%), ε4/3(20%), ε4/4(1.9%). The relative frequencies of each allele are as follow: ε2 (0.038), ε3 (0.833), ε4 (0.128). The results show as follows: ⅰ) The IHD experiment group to have a higher occurrence of ε4/3 phenotypes as well as ε4 alleles than the control group. ⅱ) Both the control group and IHD group showed the largest distribution of ε3/3 for phenotypes, and ε4 for alleles. ⅲ) The IHD group showed less ε2/3 phenotypes as well as significantly less allele frequency of ε3 in comparison to the control group. ⅳ) the IHD group showed a much lower level of HDL in comparison to the control group, while the LDL was significantly higher; samples including the apo ε2 showed a significantly higher level of HDL than those without. Among the control group, samples including apo ε2 showed a significantly higher level of TG (triglyceride) than samples without. No significant difference was found between the experiment apo ε4 sample and the control plasma lipid sample. ⅴ) No significant correlation was found between an apo E polynorphism and the number of involved arteries of a coronary angiogram. Conclusion: Between the experiment IHD group and control group were found differences in the frequency of alleles. The polymorphism of apo E alleles may contribute as a risk factor to the development of heart disease by involving itself in the metabolism and modulation of plasma lipids.

영어작문 지도의 이론과 실제

박민선,곽동기,정은정 신라대학교 교육과학연구소 2004 교육과학연구 Vol.- No.9

축산폐수처리장의 운영실태 및 개선방안에 관한 연구

정팔진,곽동희,조선영,전민식 전북대학교 공학연구원 ( 구 전북대학교 공업기술연구소 ) 2002 工學硏究 Vol.33 No.-

This study was carried out to gain operating conditions and performance improvement in J livestock treatment facility. In order to livestock wastewater, Hybrid system (biological treatment and physicochemical treatment) was operated with B3 system and advanced treatment system. Conclusions of this study were as follows: 1. COD and TN was excess design removal efficiency in treatment process 2. Influent quality was are fluctuating sharply 3. The result of biodegradability test was NBD 50%, then was led to biological treatment limit.

Lutembacher 증후군 환자 증례 보고

김성범,서정주,곽철훈,김상민,이보라,민선경,황은구,김용인,조욱현,최석구 인제대학교 2008 仁濟醫學 Vol.29 No.-

Lutembacher's syndrome is rare combination of mitral stenosis (MS) and atrial septal defect (ASD). The hemodynamic interplay between the MS and ASD leads to wide variation of clinical presentation. Here we describe a 43-year-old female with Lutembacher's syndrome and tricuspid regurgitation with pulmonary hypertension, who underwent direct closure of ASD and tricuspid valvuloplasty successfully. We also reviewed other literatures in an effort to increase awareness of this condition.

중공업 제조업체 근로자의 건강증진행위에 영향을 미치는 요인

이명화,이지현,전민경,곽선령 고신대학교 의학부 2001 高神大學校 醫學部 論文集 Vol.16 No.1

Background Heavy Industrial worker feels suffer mental stresses which are caused by heavy work, noisy environment. Such stresses influence health of the workers negatively. Thus the health promotion policy for heavy industrial workers should be made considering the workers' ways of living. This study attempted to provide basic information for development of the health promotion program for heavy industrial workers by examining predictive factors influencing health promotion behaviors of those workers. Method Data were collected from May 8th to May 27th, 1999 using questionnaires with helps of safety and health managers of the plants. Means for the study included the measurement tool of health promotion behavior provided by Park(1995), the tool of self-efficacy measurement by Suh(1995), the tool of locus of control measurement by Oh(1987), the measurement tool of perceived health state by Park(1995) and the tool of self-esteem measurement by Miller(1995). The collected data were analyzed frequency, percentage, standard deviation, t-test. ANOVA Pearson's Correlation Coefficient, multiple stepwise regression. Result Results are summarized as follows. 1. average score of health promotion behaviors 2.63±0.36(min.:1.68, max. : 3.86). In 5 areas of health promotion behavior, it showed the highest level self-realization 3.10±.42 followed by harmonious interrelationship, 2.78±.40 stress(2.55±.49), exercise and nutrition(2.46±.54), resposibility of health(2.22±.47). 2. Relations between demosociographical facters and health promotion behaviors were showed significant differences according to income(F=3.61, P=.007), age(F=3.85, P=.011). 3. Corelation between perceived factors and health promotion behavior the performance was significantly positive with self-esteem(r=.639, P=.000), and perceived health state(r=.559, P=.00) and self-efficiency(r=.557, p=.000) internal locus of control(r=.309, P=.000), external locus of control(r=.233, P=.000). 4. the Self-esteem 40.8% perceived health state, external locus of control, total carreer, carrer, self-efficacy were identified as predictor variables of health promotion behaviors 60.1%. Conclusion In conclusion, the predictive factor which most influence the performance of health promotion behaviors by heavy industrial workers was self-esteem. To promote the health, therefore, it is necessary to develop the nursing intervention program considering predictor variables identified in this study. Further industrial nurses should play their roles actively to help heavy industrial workers increase their capability of self-management of health.

서울의 PPNG 발생 빈도(1991-1992)

김재홍,윤기범,박평원,김영진,전경민,김영태,김중환,곽호,구상완,송민석,유옥,지혜구,김동원,문상은,박영립,정승호,성범진,성순제,엄주용,황정열,이기홍,이주협,전태진 대한화학요법학회 1994 대한화학요법학회지 Vol.12 No.1

The prevalence of PPNG among pretreated gonorrhea cases isolated at the STD clinic of Choong-Ku Public Health Center in Seoul has been studied and reported annually since 1981. In 1991, 123 strains of N.gonorrhoeae were isolated, among which 58(47.1%) were PPNG. In 1992, 98 starains of N.gonorrhoeae were isolated, among which 51(52.0%) were PPNG. In all, 109(49.3%) strains were found to be PPNG among 221 strains isolated between 1991-1992. The prevalence of PPNG in Seoul showed increased tendency till 1989, thereafter, it has been stationary or slightly decreasing.

Case Report : Development of Spontaneous Bacterial Peritonitis after Extended Hepatic Resection in a Patient without Evidence of Liver Cirrhosis

( Min Sun Kwak ),( Jeong Hoon Lee ),( Yoon Jun Kim ),( Jung Hwan Yoon ),( Hyo Suk Lee ) The Editorial Office of Gut and Liver 2010 Gut and Liver Vol.4 No.1

Hilar cholangiocarcinomas are often treated with liver resections. Hepatic dysfunction and infection are common postoperative complications. Although secondary bacterial peritonitis due to abdominal abscess or perforation is common, we report herein the first case of spontaneous bacterial peritonitis after hepatic resection. A 61-year-old male patient without underlying liver disease was diagnosed as having a Klatskin tumor, and a right trisectionectomy with caudate lobectomy was performed. From postoperative days 18-28, the patient gained 4.1 kg as ascites developed, and showed evidence of hepatic insufficiency with prolonged prothrombin time and jaundice. Computed tomography, performed at postoperative day 28 when fever had developed, showed only ascites without bowel perforation or abscess. When paracentesis was performed, the serum-ascites albumin gradient was 2.3 g/dL, indicating portal hypertension, and the ascites` polymorphonuclear cell count was 1,156/mm3. Since the clinical, laboratory, and image findings were compatible with spontaneous bacterial peritonitis, we started empirical antibiotics without additional intervention. Follow-up analysis of the ascites after 48 hours revealed that the polymorphonuclear cell count had decreased markedly to 108/mm3; the fever and leukocytosis had also improved. After 2 weeks of antibiotic treatment, the patient recovered well, and was discharged without any problem. (Gut Liver 2010;4: 129-134)

HCC : Risk factors, clinical features and prognosis of the hepatocellular carcinoma with peritoneal seeding (초)

( Min Sun Kwak ),( Jeong Hoon Lee ),( Jung Hwan Yoon ),( Su Jong Yu ),( Eun Ju Cho ),( Eun Sun Jang ),( Yoon Jun Kim ),( Hyo Suk Lee ) 대한간학회 2011 Clinical and Molecular Hepatology(대한간학회지) Vol.17 No.3(S)

Original Articles : Serum bilirubin Levels are inversely associated with nonalcoholic fatty Liver disease

( Min Sun Kwak ),( Dong Hee Kim ),( Goh Eun Chung ),( Seung Joo Kang ),( Min Jung Park ),( Yoon Jun Kim ),( Jung Hwan Yoon ),( Hyo Suk Lee ) 대한간학회 2012 Clinical and Molecular Hepatology(대한간학회지) Vol.18 No.4

Background/Aims: Serum bilirubin exerts antioxidant and cytoprotective effects. In addition, elevated serum bilirubin levels are associated with a decreased risk of metabolic and cardiovascular diseases. However, few studies have evaluated whether serum bilirubin is associated with non-alcoholic fatty liver disease (NAFLD), which is closely associated with other metabolic diseases. The aim of this study was thus to elucidate the association between serum total bilirubin levels and NAFLD. Methods: A cross-sectional study of 17,348 subjects undergoing a routine health check-up was conducted. Subjects positive for hepatitis B or hepatitis C virus, or with other hepatitis history were excluded. NAFLD was diagnosed on the basis of typical ultrasonographic findings and an alcohol consumption of less than 20 g/day. Results: The mean age of the subjects was 49 years and 9,076 (52.3%) were men. The prevalence of NAFLD decreased steadily as the serum bilirubin level increased in both men and women (P<0.001 for both). Multivariate regression analysis adjusted for other metabolic risk factors showed that serum bilirubin level was inversely associated with the prevalence of NAFLD [odds ratio (OR)=0.88, 95% confidence interval (CI)=0.80-0.97]. Furthermore, there was an inverse, dose-dependent association between NAFLD and serum total bilirubin levels (OR=0.83, 95% CI=0.75-0.93 in the third quartile; OR=0.80, 95% CI=0.71-0.90 in the fourth quartile vs. lowest quartile, P for trend <0.001). Conclusions: Serum bilirubin levels were found to be inversely associated with the prevalence of NAFLD independent of known metabolic risk factors. Serum bilirubin might be a protective marker for NAFLD. (Clin Mol Hepatol 2012;18:383-390)

Performance Evaluation of the Abbott Alinity hs Blood Slide Maker/Stainer

Kwak Min-Sun,Jeong In-Hwa,Cho Sung-Sook,Woo Kwang-Sook,Han Jin-Yeong 대한진단검사의학회 2022 Annals of Laboratory Medicine Vol.42 No.4