Identification of Genes Modulated by High Extracellular Calcium in Coculture of Mouse Osteoblasts and Bone Marrow Cells by Oligo Chip Assay

Kim, Hyung-Keun,Song, Mina,Jun, ji-Hae,Woo, Kyung-Mi,Kim, Gwan-Shik,Baek, Jeong-Hwa The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Calcium concentration in the bone resorption lacunae is high and is in the mM concentration range. Both osteoblast and osteoclast have calcium sensing receptor in the cell surface, suggesting the regulatory role of high extracellular calcium in bone merabolism. In vitro, high extracellular calcium stimulated osteoclastogenesis in coculture of mouse osteoblasts and bone marrow cells. Therefore we examined the genes that were commonly regulated by both high extracellular calcium and 1,25(OH)_(2)vitaminD_(3)(VD3) by using mouse oligo 11 K gene chip. In the presence of 10 mM[Ca^(2+)]e or 10 nM VD3, mouse calvarial osteoblasts and bone marrow cells were co-cultured for 4 days when tartrate resistant acid phosphatase-positive multinucleated cells start to appear. Of 11,000 genes examined, the genes commonly regulated both by high extracellular calcium and by VD3 were as follows; 1) the expressions of genes which were osteoclast differentiation markers or were associated with osteoclastogenesis were up-regulated both by high extracellular calcium and by VD3; trap, mmp9, car2, ctsk, ckb, atp6b2, tm7sf4, rab7, 2) several chemokine and chemokine receptor genes such as sdf1, scya2, scyb5, scya6, scya8, scya9, and ccr1 were up-regulated both by high ectracellular calcium and by VD3, 3) the genes such as mmp1b, mmp3 and c3 which possibly stimulate bone resorption by osteoclast, were commonly up-regulated, 4) the gene such as c1q and msr2 which were related with macrophage function, were commonly down-regulated, 5) the genes which possibly stimulate osteoblast differentiation and/or mineralization of extracellular matrix, were commonly down-regulated;slc8a1, admr, plod2, lox, fosb, 6) the genes which possibly suppress osteoblast differentiation and/or mineralization of extracellular matrix, were commonly up-regulated;s100a4, npr3, mme, 7) the genes such as calponin 1 and tgfbi which possibly suppress osteoblast differentiation and/or mineralization of extracellular matrix, were up-regulated by high extracelluar calcium but were down-regulated by VD3. These results suggest that in coculture condition, both high extracellular calcium and VD3 commonly induce osteoclastogenesis but suppress osteoblast differentiation/mineralization by regulating the expression of related genes.

자료포락분석(DEA)를 이용한 보건의료의 효율성 측정

김남송,박홍섭,김은진,김설화,김정숙,김현아,김경숙,신지혜,최은정,양은진,유선미,유수진 圓光大學校 醫科學硏究所 2007 圓光醫科學 Vol.22 No.1

알쯔하이머형 치매와 혈관성 치매환자에서의 ALDH2와 APOE 유전자의 다형성에 관한 연구

김동홍,김영돈,이창화,엄기춘,김은식,윤경식,김동희 대한생물치료정신의학회 2002 생물치료정신의학 Vol.8 No.2

연구목적: 본 연구는 국내 치매환자들을 대상으로 치매발병의 위험요소가 될 수있는 ALDH2와 APOE 유전자형이 어떤 양상으로 나타나는지 알아보기 위하여 시행되었다. 방 법: 충남 부여군에 위치한 부여노인병원에 입원중인 65세 이상의 알쯔하이머형 치매와 혈관성 또는 혼재성 치매환자 59명(남:19, 녀:40)을 대상으로 ALDH2와 APOE 유전자형을 제한효소법에 의하여 조사하였고 치매유형에 따른 유전자형과 대립유전자의 빈도를 비교하였다. 결 과: 1) 알쯔하이머형 치매환자군에서 혈관성 또는 혼재성 치매환자군보다 ALDH2*(-/2)유전자형의 빈도가 높았으나 통계학적으로 의미있는 차이는 없었다. 2) 알쯔하이머형 치매화자군에서 혈관성 또는 혼재성 치매환자군보다 APOE*(-/ε4) 유전자형의 빈도가 높았으나 통계학적으로 의미있는 차이는 없었다. 3) APOE*ε4와 ALDH2*2를 알쯔하이머병의 균등한 위험대립유전자로 가정하고 각 유전자형의 대립유전자의 수를 점수화하여 각 치매환자군의 위험유전자 전체점수를 비교한 결과 알쯔하이머형 치매환자군에서 점수가 더 높게 나왔으나 통계학적으로 의미있는 차이는 없었다. 결 론: 알쯔하이머형 치매환자군에서 혈관성 또는 혼재성치매치매환자군보다 ALDH2*2와 APOE*ε4 대립유전자의 빈도가 높게 나왔으나 통계학적으로 의미있는 차이는 없었다. A study on gene polymorphism of ALDH2 and APOE in patients with alzheimer's disease and vascular or mixed dementia. Objective : This study was designed to investigate gene polymorphism of ALDH2 and APOE, reported the risk factor of Alzheimer's disease, in korean dementia patients. Method : 59 Patients(male:19, female:40) with dementia, aged 65 and older in Buyeo geriatric hospital located in Buyeo-gun, chungnam, were finally included in this study. gene polymorphism of ALDH2 and APOE was typed with polymerase chain reaction in patients with Alzheimer's desease and vascular or mixed dementia. Results : 1) There was no difference in allelic frequency of ALDH2 gene polymorphism between patients with Alzheimer's disease and vascular or mixed dementia. 2) There was no difference in allelic frequency of APOE gene polymorphism between patients with Alzheimer's disease and vascular or mixed dementia. 3) Supposing APOE*ε4 and ALDH2*2 were equal risk allele of Alzheimer's disease, the sum of score by counting each risk allele was higher in patients with Alzheimer's disease than vascular mixed dementia, however there was no significant difference. Conclusion : The genotype frequency of ALDH2*(-/2) and APOE*(-/ε4) was higher in patients with Alsheimer's disease than vascular or mixed dementia, however there was no significant difference in allelic frequency of gene polymorphism of ALDH2 and APOE between patients with Alzheimer's disease and vascular or mixed dementia.

민들레 잎과 뿌리 분말을 첨가한 설기떡의 품질 특성

유경미,김세희,장정화,황인경,김경임,김성수,김영찬 한국조리과학회 2005 한국식품조리과학회지 Vol.21 No.1

In order to develop a dandelion (Taraxacum officinale) with natural food applications, the effects of dandelion leaves and roots powder content on the physical, textural and sensory properties of sulggidduk were examined. Dandelion powders of leaves and roots at 0, 1, 2 and 3% were added to the dandelion sulgidduk. In the dandelion roots powder in sulgidduk, the color intensity of L values and the values and moisture content decreased however, the sensory color intensity, dandelion flavor and overall acceptability increased with increasing the dandelion powder content. The sensory overall acceptability indicated the dandelion roots powder content at 3% had the highest overall and flavor scores. As the dandelion leaves content was increased, the moisture contents, lightness, adhesiveness and cohesiveness of sulggidduk decreased, while the sensory color intensity, sensory hardness, hardness of texture properties and dandelion flavor increased. The addition at 1% of the dandelion leaves powder content with the dandelion sulggidduk and the addition at 3% of the dandelion roots powder content with the dandelion sulggidduk showed the highest overall acceptability.

유아의 창의적 성격과 자아개념 간의 관계

김재희(Kim Jae Hee),이경화(Lee Kyung hwa) 숭실대학교 영재교육연구소 2013 Global Creative Leader Vol.3 No.2

본 연구는 유아의 창의적 성격과 자아개념 간에 어떠한 관계가 있는 지를 확인하는데 목적을 두었다. 연구대상으로는 서울·경기 지역에 위치한 유치원에 재원중인 만 5세 유아 70명을 선정하였다. 측정도구로는 ‘유아용 통합 창의성 검사’(이경화, 2012) 중에서 ‘창의적 성격 검사’와 ‘유아용 자아개념 검사’(이경화, 고진영, 2006)를 실시하였다. 자료처리는 유아의 창의적 성격과 자아개념간의 관계를 알아보기 위해 Pearson의 적률상관분석을 진행하였다. 자아개념 수준에 따른 창의적 성격의 차이는 자아개념을 상·하위 집단(M±1/2SD)으로 나누어 t-test를 실시하였다. 또한 유아의 성차에 따른 창의적 성격과 자아개념의 차이를 알아보기 위해 평균차이를 검증하였다. 연구결과 유아의 창의적 성격은 자아개념과 유기적인 관계를 가지며, 유아의 성차에 따라 창의적 성격과 자아개념에도 차이가 있다는 것을 알 수 있었다. 이러한 연구 결과는 창의적 성격과 자아개념 발달의 결정적인 시기인 유아를 위해 다각적인 프로그램 개발하여 제공해야 함을 시사한다. The purpose of this study was to investigate the relationship between self-concept and creative personality of young children. The participants in this research are seventy five-year old children of kindergartens in Seoul or Gyeonggi, The self-concept and creative personality were assessed by ‘Integrated creativity test for young children’ by Kyung-Hwa Lee (2012), and ‘The self-concept test for children’ developed by Kyung-Hwa Lee and Jin-Young Koh (2006). To evaluate the relation between self-concept and creative personality of young children, Pearson’s product-moment correlation analysis was implemented using SPSS Win 20.0 program. To analyze the differences of the creative personality according to self-concept of children, young children were divied into two groups (M±1/2SD) based on the descriptive analysis of collected self-concept data, then t-test was implemented. To investigate the differences of the creative personality and self-concept according to gender of children, t-test was implemented. This study shows that some elements of the self-concept related to creative personality of young children and statistically significant difference of creative personality and self-concept according to gender of children. Thus, this research could suggest valuable information about developing of multilateral creative personality and self-concept program.

유아의 다중지능 향상을 위한 요리 활동프로그램의 효과 검증

김혜란 ( Hye Ran Kim ),이경화 ( Kyung Hwa Lee ),서호찬 ( Ho Chan Seo ) 국제뇌교육종합대학원대학교 뇌교육연구소 2014 뇌교육연구 Vol.14 No.-

본 연구는 유아기의 다중지능 발달의 중요성을 인식하고 유아를 위한 효율적인 교육프로그램을 제공할 수 있는 기초자료를 확보하고자 요리활동 프로그램을 제작하고 적용해봄으로써 요리활동이 유아의 다중지능 향상에 미치는 영향을 알아보는데 교육적 의의를 가진다. 연구의 대상자는 서울시에 소 재한 S유치원의 만 5세 유아 30명을 무선 선정한 후 실험집단과 통제집단에 15명씩 무선 배치하였다. 연구도구는 만 3, 4, 5세를 대상으로 개발하여 표준화시킨 유아 다중지능 검사 도구를 사용하였으며, 수집된 자료는 사전·사후 검사로 t-test를 실시하여 집단 간의 차이를 확인하였다. 8주간의 요리활동으로 실험집단과 통제집단을 비교하여 볼 때 실험집단에서 유아들의 다중지능 향상에 도움을 주었으며 구체적으로 언어 지능, 논리·수학 지능, 시각·공간 지능, 신체운동감각 지능, 음악 지능, 개인이해 지능, 자연탐구 지능에 각각 유 의미한 결과를 나타내었다. 이상과 같은 결과로 요리활동은 유아의 오감을 모두 사용하는 최적의 활동으로 지식이나 기술 습득뿐 아니라 공감과 만족감을 맛볼 수 있다는 점에서 가치 있는 활동으로 제시될 수 있으며 유아 개개인이 지니고 있는 각자의 다 양한 지적 특성에 적합한 교육적 경험의 기회로 요리활동이 활용되어지기를 기대한다. This study has its educational significance in the sense that I have tried to identify the importance of multiple intelligence development during ch ildhood and find out whether or not children``s multiple intelligence can be improved by such programs as cooking activities that have been produced and applied for the purpose of securing basic materials that can provide young children with efficient programs. To attain at its gold of study, I have established the following assumptions and verified its effects. The targets of research were randomly selected from 5-aged 30 children of S kindergarten located in Seoul and 30 of them were arranged into a test group and a control group. The measurement in this research were taken using inspection tools for children``s multiple intelligence developed and standardized for ages of 3, 4, and 5 by Kyung-Hwa Lee. The collected materials have been processed using t-test to find out effect of pre and post-examination through t-test in order to verify significance between those two groups. These are summaries of the result carried out on this study. At first, cooking activities have had impact on children``s linguistic intelligence, showing significant difference statistically, therefore cooking activities having always positive effect on improvement of children``s linguistic intelligence. Second, cooking activities have had impact on children``s logics`` math intelligence, showing significant difference statistically, therefore cooking activities having always positive effect on improvement of children``s logics`` math intelligence. Third. cooking activities have had impact on children``s visual`` spacial intelligence, showing significant difference statistically, therefore cooking activities having always positive effect on improvement of children``s visual`` spacial intelligence. Fourth, cooking activities have had impact on children``s music intelligence, showing significant difference statistically, therefore cooking activities having always positive effect on improvement of children``s music intelligence. Fifth, cooking activities have had impact on children``s inter-personal intelligence, showing significant difference statistically, therefore cooking activities having always positive effect on improvement of children``s inter-personal intelligence. Sixth, cooking activities have had impact on children``s personal understanding intelligence, showing significant difference statistically, therefore cooking activities having always positive effect on improvement of children``s personal understanding intelligence. As a result of taking universal consideration of such results of the study, cooking activities is regarded as a worthy activity not only for acquiring knowledge or technology as an optimal activity that uses all of children``s five senses, but also for experiencing successive feeling and satisfaction. Accordingly, I hope that these cooking activities might be utilized as a educational opportunity from which children enjoy various intellectual characteristics of their own.

화병(火病)을 동반한 갱년기 환자 치험 5례

송유림 ( Yu-rim Song ),박경미 ( Kyung-mi Park ),양승정 ( Seung-jeong Yang ),이은규 ( Eun-kyu Lee ),김혜화 ( Hye-hwa Kim ),조성희 ( Seong-hee Cho ) 대한한방부인과학회 2016 大韓韓方婦人科學會誌 Vol.29 No.4

Objectives: The purpose of this study is to evaluate the effects of Korean traditional treatments for Climacteric Syndrome Patients with Hwa-Byung. Methods: From February 2015 to May 2015, We treated 5 Cases Patients with Korean traditional treatments for a month. We measured treatment effects by Menopause Rating Scale (MRS), Beck`s Depression Inventory (BDI), Visual Analogue Scale (VAS) and Digital Infrared Thermal Imaging (DITI). Results: After treatment, First, the scores of MRS, BDI were decreased. Second, the symptoms such as hot flush, pantalgia, palpitation, insomnia, fatigue were significantly improved. Also, the difference of ?中 (CV17, Danjung) and 關元 (CV4, Guanyuan) temperatures were significantly decreased after treatment. Conclusions: This study suggests that Korean traditional treatments such as Gamicheonglijagam-hwan, acupuncture, moxibustion are effective on Climacteric Syndrome Patients with Hwa-Byung.

토끼 복부 내벽으로부터 분리된 경락으로 믿어지는 관조직

손준형 ( Joon Hyung Sohn ),윤진하 ( Jin-ha Yoon ),김영주 ( Young Joo Kim ),김민경 ( Min Kyung Kim ),김지화 ( Ji Hwa Kim ),권오현 ( Ohyun Kwon ),김현원 ( Hyun-won Kim ) 대한미용학회(구 대한미용과학회) 2016 대한미용학회지 Vol.12 No.4

Investigation of meridian line and acupoints as anatomical entities should be very important, as acupuncture and meridian massage for medical purpose in the hospital or for skin care in beauty shop are each based on the traditional meridian theory. We isolated tubular structures under the skin of rabbits where the traditional meridian lines are located. The characteristics of these tubular structures isolated from the abdominal wall matches those of Bonghan tubes and Bonghan tubules found by Bong Han Kim. The Bonghan theory established by Bong Han Kim suggests that meridian lines consist of Bonghan tubes and Bonghan tubules. We could observe the presence of large cells inside the Bonghan tubule and the presence of small granules referred to as Sanal in Bonghan theory inside the striae consisting of the Bonghan tubules. Present research suggests that there exist a third unique tubular structure where Sanals flow other than Bonghan tube and Bonghan tubules.

사상체질별 비만요인에 대한 환자-대조군 연구

이갑수,석재화,김상혁,김윤희,이의주,김달래,고병희,이수경,Seok, Jae-Hwa,Kim, Sang-Hyuk,Kim, Yun-Hee,Lee, Eui-Ju,Kim, Dal-Lae,Koh, Byung-Hee,Lee, Soo-Kyung 사상체질의학회 2007 사상체질의학회지 Vol.19 No.2

1. Backgrounds and Aims Human being is divided into 4 constitution types(Taeyangin, Soyangin, Taeumin, and Soumin). According to Sasang Constitutional Medicine[SCM] Each constitution has its own incidence, treatment and prevention of disease. The purpose of this study is to find the relation of dietary habits, life styles and Psychosocial stress with obesity in each Sasang Constitution. 2. Methods 975 subjects who have received health examinations and diagnosis of Sasang Constitution at Kyung-hee Medical Center were divided into each constitutional group. Each constitutional group was then divided into obese group and normal group. We evaluated risk factors of obesity such as dietary habits, life styles, and Psychosocial stress. These data were statistically analysed to investigate the relations between risk factors and obesity. And then significant factors were analysed by multinomial logistic regression analysis to get each odds ratio. 3. Results In a multinomial logistic regression analysis adjusted for age and sex, promotion of appetite and overeating or excessive diet elevated risks of obesity in Taeumin, promotion of appetite and high speed of diet elevated risks of obesity in Soyangin, and Psychosocial stress elevated risks of obesity in Soeumin. 4. Conclusions The results suggest that promotion of appetite and overeating or excessive diet may be major risk factors for obesity in Taeumin, promotion of appetite and high speed of diet in Soyangin and Psychosocial stress and dyschezia in Soeumin.

지식기반사회의 학교 교육과정 개발을 위한 기초연구로서의 제7차 초등수학과 교육과정 분석

김경자,정미화 이화여자대학교 사범대학 교과교육연구소 2001 교과교육학연구 Vol.5 No.2

이 연구에서는 지식기반사회에서의 수학과 교육과정을 개발하기 위한 함의를 도출하기 위해 2000년부터 점진적으로 실시되고 있는 제7차 초등수학 교육과정을 분석하였다. 분석대상을 국가수준의 교육과정 문서인 교육부 고시 초등수학 「교육과정」과 「교육과정해설」 및 「교사용 지도서」로 삼았고, 분석결과는 크게 (1) 구성과 제시형식 분석, (2) 내용분석으로 구분하여 진술하였으며, 내용분석은 다시 성격, 목표, 내용, 방법, 평가의 순으로 기술하였다. 제7차 초등수학 교육과정을 분석한 결과, 국가수준의 초등수학 교육과정 문서는 주된 독자인 교사들의 이해를 돕는 형식을 갖추어야 하고, 내용에 있어서도 교사들에게 수학이 무엇이고, 수학 수업에서 무엇이 가르쳐져야하며, 또 어떻게 가르쳐야 할 것인가에 대한 명확한 이해를 주는 관점의 명시와 내용 구조 그리고 구체적 접근 방법에 대한 상세화가 필요한 것으로 나타났다. The purposes of this study were to analyze the 7th amended math curriculum for elementary schools to draw implications for improvement of it. The curriculum documents published by the Ministry of Education in 1997 were studied. Forms and contents of the documents were analyzed. The forms were studied in terms of feasibility. The content was explored focusing on the nature of math, goals and objectives, content, learning activities, and evaluation. The results were as follows. (1) The national math curriculum consists of two volumes. One is a summarized version and another is an interpreted version. It was found that separation of two volumes did not have any meaning in terms of feasibility and usability. (2) Because perspectives on the curriculum describing why it had been developed and the nature of the mathe curriculum was not clearly presented, goals and objectives, content, learning activities, and evaluation could be interpreted differently depending on the reader's perspectives or philosophy. The recommendations for improvement of the curriculum were suggested as follows. (1) Considering teachers as the major audience of the curriculum, perspectives on the curriculum and the nature of math curriculum should be more explicitly stated so that teachers can understand the curriculum in a consistent manner and as a result use it easily. (2) Internal consistency among curricular elements such as goals and objectives, content, learning activities, and evaluation should be more firmly secured in accordance with the nature of math should be more assured.