곤충세포 - 빼큘로바이러스 시스템에서 제조합단백질 생산을 위한 누에체액의 이용

배수미,하성호,박태현 ( Su Mi Bae,Sung Ho Ha,Tai Hyun Park ) 한국화학공학회 1996 화학공학의이론과응용 Vol.2 No.1

염증유도 RAW264.7 세포와 동물모델에서 구기자와 구기엽의 항염 효능

배수미,김지은,배은영,김경아,이선영 한국영양학회 2019 Journal of Nutrition and Health Vol.52 No.2

Purpose: Medicinal herbs have recently attracted attention as health beneficial foods and source materials for drug development. Recent studies have demonstrated that extracts of Lycium’s fruits and roots have a range of physiologically active substances. The extract of Lycium’s leaves has been reported to have excellent anti-oxidant and anti-microbial activity, but its anti-inflammatory efficacy is not known. The chlorophyll present in the leaves can act as an anti-oxidant or pro-oxidant depending on the presence of light. Therefore, this study analyzed the anti-inflammatory effects of Lycium’s fruit extract (LFE), leaf extract (LLE), and leaf extract with chlorophyll removal (LLE with CR). Methods: This study examined the inhibitory effects of LFE, LLE, and LLE with CR on pro-inflammatory mediator production as well as on the expression of iNOS and COX-2 in lipopolysaccharide (LPS)-stimulated RAW264.7 cells and BALB/c mice. Results: LFE, LLE, and LLE with CR inhibited the production of pro-inflammatory mediators (NO, TNF-α, IL-6, and IL-1β) and the expression of iNOS and COX-2 in LPS-stimulated RAW 264.7 cells in a dose-dependent manner. Furthermore, the administration of LLE and LLE with CR inhibited the serum pro-inflammatory cytokine levels and suppressed DNA damage in BALB/c mice. In particular, LLE with CR exhibited the highest anti-inflammatory activity. Conclusion: These results suggest that the fruit and leaves of Lycium are potential therapeutic agents against inflammation.

Protein Expression Profile using Two-Dimensional Gel Analysis in Squamous Cervical Cancer Patients

배수미,민현진,Guo Hua Ding,곽선영,조영래,남계현,박충학,김용완,김종국,한병돈,이영주,김도강,안웅식 대한암학회 2006 Cancer Research and Treatment Vol.38 No.2

Purpose: Screening in cervical cancer is now progressingto discover candidate genes and proteins that mayserve as biological markers and that play a role in tumorprogression. We examined the protein expression patternsof the squamous cell carcinoma (SCC) tissues fromKorean women with using two- dimensional polyacrylamidegel electrophoresis (2-DE) and matrix assistedlaser desorption/ionization-time of flight (MALDI- TOF)mass spectrometer.Materials and Methods: Normal cervix and SCC tissueswere solubilized and 2-DE was performed using pH 3~10linear IPG strips of 17 cm length. The protein expressionwas evaluated using PDQuest 2-D softwareTM. The differentiallyexpressed protein spots were identified with aMALDI-TOF mass spectrometer, and the peptide massspectra identifications were performed using the Mascotprogram and by searching the Swiss-prot or NCBInrdatabases.Results: A total of 35 proteins were detected in SCC.17 proteins were up-regulated and 18 proteins weredownregulated.Among the proteins that were identified, 12proteins (pigment epithelium derived factor, annexin A2and A5, keratin 19 and 20, heat shock protein 27, smoothmuscle protein 22 alpha, α-enolase, squamous cell carcinomaantigen 1 and 2, glutathione S-transferase andapolipoprotein a1) were protein previously known to beinvolved in tumor, and 21 proteins were newly identifiedin this study.Conclusion: 2-DE offers the total protein expressionprofiles of SCC tissues; further characterization of thesedifferentially expressed proteins will give a chance toidentify the badly needed tumor-specific diagnostic markersfor SCC. (Cancer Res Treat. 2006;38:99-107) Purpose: Screening in cervical cancer is now progressing to discover candidate genes and proteins that may serve as biological markers and that play a role in tumor progression. We examined the protein expression patterns of the squamous cell carcinoma (SCC) tissues from Korean women with using two- dimensional polyacrylamide gel electrophoresis (2-DE) and matrix assisted laser desorption/ionization-time of flight (MALDI- TOF) mass spectrometer.Materials and Methods: Normal cervix and SCC tissues were solubilized and 2-DE was performed using pH 3~10 linear IPG strips of 17 cm length. The protein expression was evaluated using PDQuest 2-D softwareTM. The differentially expressed protein spots were identified with a MALDI-TOF mass spectrometer, and the peptide mass spectra identifications were performed using the Mascot program and by searching the Swiss-prot or NCBInr databases.Results: A total of 35 proteins were detected in SCC.17 proteins were up-regulated and 18 proteins weredownregulated.Among the proteins that were identified, 12 proteins (pigment epithelium derived factor, annexin A2 and A5, keratin 19 and 20, heat shock protein 27, smooth muscle protein 22 alpha, α-enolase, squamous cell carcinoma antigen 1 and 2, glutathione S-transferase and apolipoprotein a1) were protein previously known to be involved in tumor, and 21 proteins were newly identified in this study.Conclusion: 2-DE offers the total protein expression profiles of SCC tissues; further characterization of these differentially expressed proteins will give a chance to identify the badly needed tumor-specific diagnostic markers for SCC. (Cancer Res Treat. 2006;38:99-107)

Lycium Chinense의 열매 추출물과 잎 추출물의 항염 효능 연구

배수미(Su-Mi Bae),남유리(You-ree Nam),김지은(Ji-Eun Kim),서진형(Jin-hyung Suhr),이선영(Sun Yung Ly) 한국생활과학회 2018 한국생활과학회 학술대회 Vol.2018 No.5

Antiproliferative Effects of Quercetin through Cell Cycle Arrest and Apoptosis in Human Breast Cancer MDA-MB-453 Cells

최은정,배수미,안웅식 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.10

To explore the anticancer effects of the flavonoid quercetin on human breast cancer MDA-MB-453 cells via cell cycle regulation and the induction of apoptosis, the antiproliferative effect of quercetin was first examined by MTT assay. When MDA-MB-453 cells were treated with quercetin for various periods of time (3-24 hrs) and at various doses (1-100 μM), cell growth decreased significantly in a time- and dosedependent manner. To elucidate the mechanism underlying the antiproliferative effect of quercetin, cell cycle progression and the induction of apoptosis in MDA-MB-453 cells exposed to 100 μM quercetin for 24 hrs were investigated. Quercetin caused a remarkable increase in the number of sub-G1 phase cells, and an Annexin-V assay revealed that exposure to quercetin affected apoptosis. Moreover, treatment with quercetin increased Bax expression but decreased Bcl-2 expression. Cleaved caspase-3 and PARP expression was also increased by quercetin. Thus, quercetin has probable anticancer activity. Our results suggest the existence of multiple pathways for the induction of cell cycle arrest and apoptosis by quercetin.

한국인 난소암 조직에서 발현된 miRNA의 타겟 mRNA를 중심으로 하는 p53 관련 신호전달 체계 분석

권지영,배수미,안웅식,김정민,서영록 대한암예방학회 2010 Journal of cancer prevention Vol.15 No.3

Ovarian cancer has been considered as the mostly common cancer among women in worldwide. Molecular mechanism of initiation and progression in this cancer has been well studied, however, diagnotic and therapeutic programs has not been successfully established yet. In order to improve screening program to detect the ovarian cancer at the early stage and subsequently develop therapeutic modalities for cancer treatment, research focusing on more insight of regulatory molecular mechanisms are urgently required. Here, we analyze effect of microRNAs (miRNAs), recognized as non-coding RNAs that negatively regulate expression of target genes, on gene expression at genome wide level. Concerning with potential miRNA features, each miRNA is able to inhibit hundreds of transcripts directly or indirectly. Therefore,signaling network analysis of miRNA targeting on particular mRNA would provide novel molecular interactions for establishing alternative approach to treat the ovarian cancer patients. Our group has analyzed miRNA microarray in ovarian tissues from Korean patients. Based on our previous microarray study, we discovered miRNA-associated target mRNAs participating in p53-regulated signaling. As result,we have presented 26 target genes related with p53-controlling pathway. Interestingly, the identified three genes encoding ING1, NDRG1 and CDC14A might be further recognized as novel target molecules in ovarian cancer. This study might provide evidence for better understanding mechanism in relevance to governing ovarian cancer and consequently for improving diagnosis and therapy of this cancer.

임신동안 GnRH/GnRHR 신호전달체계와 황체호르몬 수용체의 상호관계

이희중,배수미 대한산부인과학회 2011 대한산부인과학회 학술대회 Vol.97 No.-

클로로필을 제거한 영하구기엽 에탄올 추출물의 항산화 활성

김지은,배수미,남유리,배은영,이선영 한국영양학회 2019 Journal of Nutrition and Health Vol.52 No.1

Purpose: The aim of this study was to estimate the antioxidant activities of 50%, 70%, and 100% ethanol extracts of Lycium barbarum leaf and chlorophyll removal extract. Methods: The antioxidant activities were estimated by measuring total polyphenol content and by assays of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline- 6-sulfate) (ABTS) radical scavenging activities and ferric reducing antioxidant power (FRAP). In addition, reactive oxygen species (ROS) production, DNA fragmentation, and antioxidant enzyme (superoxide dismutase and catalase) activities of the extracts were measured in hydrogen peroxide (H2O2)-stressed HepG2 cells. Results: The total polyphenol content, DPPH and ABTS radical scavenging activities, and FRAP value of the extracts increased in an ethanol concentration-dependent manner. The antioxidant activities of the chlorophyll-removal extracts were much higher than those of the chlorophyll-containing extracts. Cytotoxicity was not observed in HepG2 cells with extracts up to 1,000 μg/mL. All extracts inhibited ROS production in a concentration-dependent manner from 31.3 μg/mL and inhibited DNA damage at 250 μg/mL. The SOD and catalase activities of cell lines treated with the extracts and H2O2 were similar to those of normal cells, indicating a strong protective effect. Conclusion: Lycium barbarum leaf extracts had high antioxidant activities and protected H2O2-stressed HepG2 cells. Since the chlorophyll-removal extract exhibited higher antioxidant activities than the chlorophyll-containing ones and the cytoprotective effect was similar, chlorophyll removal extract of Lycium barbarum leaf could be developed as ingredients of functional food and cosmetics.

정상상피세포(HaCat)와 자궁경부 암세포(SiHa)에서 GeneFishing^(TM) PCR technique을 이용한 유전자 발현의 변화

김병훈,배수미,서민제,김용완,이정웅,김용욱,이준모,남궁성은,김종국,안웅식 대한부인종양 콜포스코피학회 2003 Journal of Gynecologic Oncology Vol.14 No.4

목적 : 본 실험의 목적은 정상상피 세포와 자궁경부 암세포 사이에서 유전자의 발현 차이를 조사하였다. 연구 방법 : 정상상피 세포(HaCat)와 자궁암 세포(SiHa)를 사용하였으며, 두 세포 간에 유전자 발현 차이를 GeneFish^(TM) PCR을 이용하여 알아보았으며, BLAST serach를 통해 분석하였다. 결과 : 정상상피 세포와 자궁암 세포 비교 결과, 자궁암 세포에서 S1-2-2와 S5-1을 포함한 25개의 유전자가 발현이 증가하였고, 24개의 유전자가 감소하였다. 결론 : GeneFishing^(TM) PCR기법은 유전자의 발현 변화를 확인하는데 있어서 아주 민감하고 효과적인 방법이다. 우리는 정상상피 세포와 자궁경부 암세포에서 다르게 발현하는 유전자를 찾을 수 있었고, 앞으로는, 종양의 발생과 진행과정에 관여하는 유전자를 더 탐지하고 해당 유전자의 기능을 연구할 필요가 있다고 생각된다. Objective : The purpose of this study is investigated the differentially expressed genes between normal and cervical cancer cell line. Methods : We used normal human keratinocyte (HaCaT) as a control and HPV-16 positive cervical cancer (SiHa) cell line. Two cell lines were studied differential expressed genes by using GeneFishing^(TM) PCR and analyzed with BLAST search. Results : As compared with normal, cervical cancer cell line was showed 25 up-regulated genes including the S1-2-2, S5-1 and 24 down-regulated genes. Conclusion : GeneFish^(TM) PCR test is very sensitive and effective method for detection of changed gene expression. We could search differentially expressed genes between normal and cervical cancer cell line. In the future, we need to research various genes function to participate in the process of tumor development and progression.

Tests for Fuzzy Mean of Poisson Distribution

강만기,배수미,최규탁 한국지능시스템학회 2008 한국지능시스템학회 학술발표 논문집 Vol.18 No.2

반복적인 실험에서 관측된 데이터의 발생 빈도가 약하며 변동 폭을 포함하는 경우에 관측자료를 퍼지화하여 이에 대한 확률을 정의하고, 관측된 자료의 확률에 따른 퍼지평균을 구하여 퍼지 포아송 분포를 사용하여 평균을 동의지수법에 의한 검정법을 제안하고 예증한다. We propose some properties for fuzzy Poisson test by agreement index. First we define fuzzy probability for repeatedly observed data with alteration error term. For few mean, we show that a Poisson function of performance for a fuzzy hypothesis test and drawing conclusions from the test.