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$\bar{x}$-chart 의 경제적(經濟的) 파라메터 설정(設定)에 관한 연구(硏究)
한병돈,황의철,Han, Byeong-Don,Hwang, Ui-Cheol 한국품질경영학회 1983 품질경영학회지 Vol.11 No.1
The main factors of determining the Control Line of the Control Chart can be classified as follows: 1) sample size (n), 2) the factor that determines the spread of Control Limits (B), (3) sampling frequency (h). The determination of these factors can be explained according to the extent that occurrences of assignable cause should be detected. The purpose of this paper are two: one is for composing a model of which use should be designated for economic decision on the size of these factors leading to the Control Line of the Control Chart, the other is about what influence increasing or decreasing condition, according to changeability of the size of these factors, of expect cost can have on the economy when the Control Chart is used.
임인경,한병돈,이기호,윤택구,Lim, In-Kyoung,Han, Byoung-Don,Lee, Kee-Ho,Yun, Taik-Koo 생화학분자생물학회 1985 한국생화학회지 Vol.18 No.1
NIH(GP) 마우스 태아 세포 일차배양계에서 GST활성도와 B($\alpha$)P 대사에 미치는 BHA의 영향에 관한 실험을 수행하였다. 1-Chloro-2,4-dinitrobenzene (CDNB)을 기질로 하였을 때 GST의 최적 pH는 6.5 이었으며, 이때 반응액은 1.0 mM CDNB, 1.0 mM GSH 그러고 $50-100\;{\mu}g$의 세포질 단백으로 조성 하였다. 1,2-Epoxy-3-(p-nitrophenoxy)propane (alkyl epoxide)를 기질로 하였을 때 GST의 최적 pH는 5.8이었으며, 이때 반응액은 0.5 mM alkyl epoxide, 10 mM GSH, $50-100\;{\mu}g$의 세포잘 단백으로 조성하였다. CDNB를 기질로 하는 GST 활성도는 배양 5일에 의미있게 증가되었으며 그 활성도는 배양 7일까지 유지되었다. BHA배지에서 7 일간 배양된 세포내 GST 활성도는 대조군에 비하여 의미있게 증가하였다. $4\;{\mu}M$ $^3H-B(\alpha)P$ (500 mCi/m mol) 첨가하여 24시간 배양한 후 회수한 완전 배지와 BHA배지에서 분리되는 수용성, B(a)P 대사산물의 분획은 배양 3일에 비하여 배양 7일에 현저히 증가하였으며, 이때 BHA 첨가로 인한 영향은 없었다. B($\alpha$)P-DNA, B($\alpha$)P-RNA 그리고 B($\alpha$)P-protein adducts 양은 배양 일수나 배양액의 조건에 의하여 차이가 없었다. We examined the effect of BRA on the activities of glutathione-S-transferase(GST) and the metabolism of benzo($\alpha$)pyrene in the primary culture of embryonic fibroblasts derived from the NIH(GP) mice. Optimum pR of GST was 6.5 with 1.0 mM of 1-chloro-2, 4-dinitrobenzene, 1.0 mM of reduced glutathione, and $50-100\;{\mu}g$ of cytosol proteins in 0.1 M potassium phosphate buffer. Optimum pH of GST with 0.5 mM 1,2-epoxy-3 (p-nitrophenoxy) propane, 10 mM of GSH and $50-100\;{\mu}g$ of cytosol proteins in 0.1 M potassium phosphate buffer was found to be 5.8. The activities of GST catalyzing CDNB were increased on the 5th day of the culture and maintained on the 7th day. The culture medium containing 100\;${\mu}M$ BRA significantly increased the activities of GST with CDNB on the 7th day as compared with those of the control. The fractions of the conjugated B($\alpha$)P metabolites in both of the control and the BRA media were increased on the 7th day as compared with those on the 3rd day of the culture, after incubation with $4\;{\mu}M$ $^3H-B(\alpha)P$ (500 mCi/m mol) for 24 hours. However, the binding levels of B($\alpha$)P to the cellular DNA, RNA and proteins were not changed depending on the culture days and the culture medium. BHA had no trans-placental effect on the activities of glutathione-S-transferases and the metabolism of B($\alpha$)P in our experiment.
일차배양 마우스 태아 세포의 Glutathione - S - transferases 활동도와 Benzo ( a ) Pyrene 대사에 관한 연구
임인경,한병돈,이기호,윤택구 ( In Kyoung Lim,Byoung Don Han,Kee Ho Lee,Taik Koo Yun ) 생화학분자생물학회 1985 BMB Reports Vol.18 No.1
We examined the effect of BHA on the activities of glutathione-S-transferase(GST) and the metabolism of benzo(α)pyrene in the primary culture of embryonic fibroblasts derived from the NIH(GP) mice. Optimum pH of GST was 6.5 with 1.0 mM of 1-chloro-2, 4-dinitrobenzene, 1.0 mM of reduced glutathione, and 50-100 ㎍ of cytosol proteins in 0.1 M potassium phosphate buffer. Optimum pH of GST with 0.5 mM 1,2-epoxy-3 (p-nitrophenoxy) propane, 10 mM of GSH and 50-100 ㎍ of cytosol proteins in 0.1 M potassium phosphate buffer was found to be 5.8. The activities of GST catalyzing CDNB were increased on the 5th day of the culture and maintained on the 7th day. The culture medium containing 100 μM BHA significantly increased the activities of GST with CDNB on the 7th day as compared with those of the control. The fractions of the conjugated B(α)P metabolites in both of the control and the BHA media were increased on the 7th day as compared with those on the 3rd day of the culture, after incubation with 4 μM ³H-B(α)P (500 mCi/m ㏖) for 24 hours. However, the binding levels of B(α)P to the cellular DNA, RNA and proteins were not changed depending on the culture days and the culture medium. BHA had no transplacental effect on the activities of glutathione-S-transferases and the metabolism of B(α)P in our experiment.
c-fos Oncogene Expression in the Growth-Stimulated Cells
임인경,이기호,이도종,윤택구,한병돈,유주현,Lim, In-Kyoung,Lee, Kee-Ho,Lee, Do-Jong,Yun, Taik-Koo,Han, Byoung-Don,Yu, Ju-Hyun Korean Society for Biochemistry and Molecular Biol 1987 한국생화학회지 Vol.20 No.2
마우스 태생조직과 혈청 투여로 세포 성장을 유도시킨 NIH 3T3 마우스 섬유아세포 및 A549 사람 폐암 세포주에서 c-fos 유전자의 발현을 조사하였다. 태령 13 일과 17일된 A/J, C57BL/6J 마우스 태아, 태반 및 태아 외막과 2-3 일간 성장을 정지시킨후 15% 혈청 투여로 세포성장을 유도한 NIH 3T3 섬유아세포와 A549 폐암 세포주에서 세포 총 RNA를 분리하였다. 마우스 태아 및 관련 장기는 태령에 관계없이 c-fos의 강한 발현을 나타내였으나 NIH 3T3 세포에서는 혈청 첨가 30분 후에 c-fos 유전자 발현이 유도되었다가 2시간 이후는 대조군 수준으로 감소하였다. 한편, A549 암세포에서는 혈청 첨가에 관계없이 c-fos 유전자의 발현이 활발하였다. We describe here the expression of c-fos oncogene in the growth-stimulated cells, e.g., mouse prenatal tissues, FCS treated NIH-3T3 fibroblast and A549 human lung cancer cells. Total cellular RNA was isolated from A/J and C57BL/6J mouse embryos at the 13th and 17th day of pregnancy. RNAs isolated from mouse prenatal tissues were strongly hybridized with c-fos. NIH-3T3 mouse fibroblast and A549 lung cancer cells were stimulated by the 15% FCS after two to three days of serum depletion. During the serum stimulation, c-fos expression was detected at time intervals by the dot blot analysis. c-fos induction was detected from 30' after serum stimulation in NIH-3T3 cells, which was turned off after 2 hrs. On the other hand, c-fos induction in the A549 lung cancer cells was independent of the serum stimulation.
성장이 활발한 세포에서 c - fos 암 유전자의 발현
임인경,이기호,이도종,윤택구,한병돈,유주현 ( In Kyoung Lim,Kee Ho Lee,Do Jong Lee,Taik Koo Yun,Byoung Don Han,Ju Hyun Yu ) 생화학분자생물학회 1987 BMB Reports Vol.20 No.2
We describe here the expression of c-fos oncogene in the growth-stimulated cells, e.g., mouse prenatal tissues, FCS treated NIH-3T3 fibroblast and A549 human lung cancer cells. Total cellular RNA was isolated from A/J and C57BL/6J mouse embryos at the 13th and 17th day of pregnancy. RNAs isolated from mouse prenatal tissues were strongly hybridized with c-fos. NIH-3T3 mouse fibroblast and A549 lung cancer cells were stimulated by the 15% FCS after two to three days of serum depletion. During the serum stimulation, c-fos expression was detected at time intervals by the dot blot analysis. c-fos induction was detected from 30` after serum stimulation in NIH-3T3 cells, which was turned off after 2 hrs. On the other hand, c-fos induction in the A549 lung cancer cells was independent of the serum stimulation.
이양원,정은숙,최경영,권소영,조남선,김진숙,박한정,한병돈,윤수영 대한수혈학회 2012 大韓輸血學會誌 Vol.23 No.2
Background:Leukocyte reduction filters are widely used to prevent transfusion reactions caused by leukocytes in blood components. Commercial filters are not sufficient for removal of leukocytes for prevention of transfusion associated graft-versus-host disease; therefore, irradiation of blood components was performed using expensive equipment. Techniques using an aptamer substituted for antibody have been developed and are available in clinical areas. The purpose of this study is to develop the aptamer filter system and to evaluate its efficiency and the possibility of its clinical application. Methods:Aptamers targeted to CD45 were selected by the Postech Aptamer Initiative. The aptamer filter in which aptamers attached to beads were bound to leukocytes and removed by magnetic field was developed. Filtration of 14 units of leukoreduction-red blood provided by Korean Red Cross Blood Services was performed using aptamer filters. Leukocyte removal rate and red cell recovery rate were evaluated and bacterial culture was performed. Results:After filtration using the aptamer filters, 45.6% of leukocytes were additionally removed and the red cell recovery rate was 92.8%. No growth in the bacterial culture was observed. Conclusion:In order to apply the cell depletion technique utilizing an aptamer to blood filter system, we developed and evaluated the aptamer filter system. Through improvement of the binding efficiency of the aptamer and the filtering process, and application of the various aptamers for other different cells, we suggest that this technique can be applied in the clinical area, such as a substitution for the irradiation process for TAGVHD prevention