Hepatic Cirrhosis Occurring in a Young Woodchuck(Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus(WHV)

Kyu-shik Jeong,정원일,Jae-yong Chung,Mi-young An,Chae-yong Jung,Gyoung-jae Lee,Jong-soo Kang,Byeong-cheol Kang,Young-heun Jee,Bruce H Williams,Young-oh Kwon,Da-hee Jeong 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

Cirrhosis Occurring in a Young Woodchuck (Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus (WHV)Da-hee Jeong, Won-il Jeong, Jae-yong Chung, Mi-young An, Chae-yong Jung, Gyoung-jae Lee1, Jong-soo Kang1, Byeong-cheol Kang2, Young-heun Jee3, Bruce H Williams4, Young-oh Kwon5 and Kyu-shik Jeong*College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea1Shinwon Scientific Co., LTD, Research Institute, Suwon, Korea2Clinical Research Institute, Seoul National University, Seoul 110-744, Korea3College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea

노화가 인체 중간엽 줄기세포로부터 조골세포로의 증식 및 분화에 미치는 영향

백기현,태현정,오기원,이원영,조정기,권순용,강무일,차봉연,이광우,손호영,강성구,김춘추 대한내분비학회 2003 Endocrinology and metabolism Vol.18 No.3

연구배경: 일반적으로 골다공증과 연관된 위험인자로는 연령, 폐경, 약물, 불충분한 칼슘섭취, 만성질환 및 운동부족 등이 있는데, 특히 노화가 진행할수록 골밀도가 감소하는 것은 잘 알려져 있다. 노화와 관련하여 진행되는 골소실은 조골세포 및 전구조골세포의 기능적 결핍에 의한 골형성의 감소가 주요한 요인으로 여겨지고 있다. 그 동안 연령이 조골모 세포의 양과 조골모 세포로부터 성숙조골세포로의 분화 및 증식에 미치는 영향에 대한 일부 보고들이 있었으나 아직 일치된 견해는 없는 형편이다. 방법: 다양한 연령의 사람으로부터 골수를 채취, 중간엽 줄기세포가 포함된 단핵세포를 분리한 후 조골세포로 분화하기 좋은 조건하에서 배양하였다. 대상군은다시 젊은군과 노령군으로 구분하여 다양한 변수를 비교 분석하였다. 일차배양에서는 CFU-F를 계수하여 골수내 중간엽 줄기세포의 수를 추산하였고, 칼슘측정을 통하여 기질의 무기화 정도를 비교하였다. 계대배양후 이차배양에서는 시기별로 알카리성 포스파타제 활성도를 측정하고 오스테오칼신 mRNA의 발현을 관찰하여 젊은군과 노령군 사이의 증식능 차이를 비교하였다. 또한 이차배양 시기별로 MTT 측정을 하여 양군간에 증식능 차이가 있는지 알아보았다. 결과: 1. 일차배양 15일째에 평균 CFU-F의 수는 젊은군에서 유의하게 많았다(젊은군 148.3±28.9, 노령군 54.3±9.1, p=0.02). CFU-F의 평균면적은 젊은군에서 넓은 경향을 보였으나 통계적으로 유의하지는 않았다. 2. 일차배양 17일 경과 후 양군간에 기질 칼슘 침착정도는 유의한 차이를 보이지 않았다(젊은군 103.6±50.6, 노령군: 114.0±56.5, p=NS). 3. 이차배양 10일째에 젊은군에서 알카리성 포스파타제 활성도가 고령군에 비해 유의하게 높았다(젊은군: 935.5±115.0 U/mg, 노령군: 578.4±115.7U/mg,p.0.05). 고령군에서는 시간 경과에 따른 변화가 미약했으며 전반적으로 알카리성 포스파타제의 활성도가 젊은군에 비해 낮았다. 4. 이차배양도중 오스테오칼신 mRNA의 발현은 배양시기별로 젊은군에 비해 고령군에서 더 낮은 경향을 관찰할 수 있었다. 5. 이차배양 10일과 15일에 젊은군에서 노령군보다세포증식이 유의하게 증가된 양상을 보였다(10앓 젊은군 0.73±0.05, 노령군 0.58±0.04, p=0.05, 15일; 젊은군 0.80±0.05, 노령군 0.70±0.03, p=0.05).결론: 이상의 연구에서 저자들은 노령군에서 젊은군보다 골수 내 중간엽줄기세포의 수가 적고, 노령군에서 유래한 전구조골세포의 성숙조골세포로의 증식 및 분화가 젊은군 보다 감소해 있는 것을 관찰할 수 있었다. Background: Osteoblasts originate from osteoprogenitor cells in bone marrow stroma, termed mesenchymal stem cells (MSCs) or bone marrow stromal cells. Each MSC forms colonies (colony forming units-fibroblasts [CFL-Fs]) when cultured ex vivo. There are some reports about the age-related changes of the number and osteogenic potential of osteoprogenitor cells, but any relationship has not been clearly established in humans. In this study, we counted MSCs using CFU-Fs count and examined the proliferative capacity and differentiation potential of osteoprogenitor cells. Finally, we analyzed how these parameters varied with donor age. Methods: Bone marrow was obtained from the iliac crest of young (n=6, 27.2±8.6 years old) and old (n= 10, 57.4k6.7 years old) healthy donors. Mononuclear cells, including MSCs, were isolated and cultured in osteogenic medium. In primary culture, we compared the colony-forming efficiency of MSCs between the two groups and determined the matrix calcification. When primary culture showed near confluence, the cells were subcultured. Alkaline phosphatase activity, osteocalcin expression by RT-PCR and proliferative potential by MTT assay were examined by the time course of secondary culture. Results: At the 15th day of primary culture, the mean number of CFU-Fs was significantly higher in the younger donors (young: 148.3±28.9, old: 54.3±9.1, p=0.02) and the mean size of CFL-Fs was also larger in the younger donors than the older donors. However, matrix calcification was not different between the two groups (young: 103.6±50.6, old: 114.0±56.5, p=NS). In secondary culture, alkaline phosphatase activities were significantly lower in the older donors. The younger donors showed peak alkaline phosphatase activity at day 10, while the older donors didn't showed a remarkable peak (young: 935.5±115.OU/mg, old: 578.4±115.7U/mg, p<0.05). Total cell number as a proliferative index increased progressively during the secondary culture and a significantly greater cell number was noted in the younger donors. Osteocalcin expression was generally upregulated in the younger donors, but this was not statistically significant. Conclusion: Our study shows that the number of osteoprogenitor cells is decreased during aging and that the proliferative capacity and differentiation potential of osteoprogenitor cells seem to be reduced during aging (J Kor SOC Endocrinol 18:296-305, 2003).

약물로 인해 유발된 좌불안석증에서 세로토닌 차단제인 Cyproheptadine의 효과

정동선,박인준,권영준,정희연,한선호 大韓神經精神醫學會 1997 신경정신의학 Vol.36 No.5

목 적 : 약물로 인해 유발된 좌불안석증은 항정신병 약물로 치료받은 환자에게서 자주 발생한다. 항세로토닌 제제인 Cyproheptadine은 좌불안석증 환자들을 치료키 위해 사용되어져 왔다. 방 법 : 21명의 항정신병 약물로 치료받은 좌불안석증을 동반한 환자들이 4일간에 걸쳐 하루 16mg의 Cyproheptadine을 투여 받았다. 좌불안석증에 대한 평가는 Barnes' rating scale을 이용하여 이루어졌다. 우울증과 정신병증상의 정도가 Brief psychiatric rating scale(BPRS)와 Hamilton rating scale for depression(HAM-D)에 의해 평가되어졌다. 결 과 :좌불안석증을 보인 대부분의 환자들이(20/21) Cyproheptadine 치료에서 호전을 보여주었다. 치료기간에 정신병이나 우울증의 악화는 없었다. Cyproheptadine 투여가 중단되었을 때 좌불안석증의 증상은 악화되었다. 결 론 : Cyproheptadine은 약물로 인해 유발된 좌불안석증 치료에 유용하다. Objective : Neuroleptic Induced Akathisia(NIA) often occurs in neuroleptic treated patients. Cyproheptadine, an antiserotonergic agent, was used to treat neuroleptic induced akathisia. Method : In an open trial 21 neuroleptic-treated patients with akathisia were administrated Cyproheptadine(16mg/day) over 4 days. Assessment of akathisia was evaluated using Barnes' rating scale(BAS) for neuroleptic induced akathisia. The degree of depression and psychosis were assessed by brief psychiatric rating scale(BPRS) and Hamilton rating scale for depression(HAM-D). Result : Most patients(20 of 21) with neuroleptic induced akathsia(NIA) showed improvement under the treatment of cyproheptadine. There was no aggravation of psychosis or depression during the treatment. Symptoms of akathisia aggravated when cyproheptadine was discontinued. Conclusion : Cyproheptadine may be useful in the treatment of neuroleptic induced akathisia(NIA).

한국의 재활의료 전달체계

권혁철,정영일,남은우 高神大學校 保健科學硏究所 1993 보건과학연구소보 Vol.3 No.-

This study was carried out for reorientation of Rehabilitation Service Delivery System (RSDS) through reviewing reference and observing field practice. The most important results are as follows: 1. The current rehabilitation facility for handicapped persons can be classified into four categories : (a) those for physically disabled persons, (b) those for mentally disturbed persons, (c) those for visually disturbed persons, and (d) those for acoustic and speech disturbed persons. The nationwide number of rehabilitation facilities is 101 of those, 43.5% is for physically disabled persons and 32.9% is for mentally disturbed persons. 2. A small number of circulating rehabilitation centers for physically disability are being run by comprehensive rehabilitation centers since 1992. 3. The hospitals are self-supporting only 20% of their operational costs, and the rest of the operational costs comes from federal tax(40%) and local tax(40%). 4. The number of handicapped persons in Taegu and Kyungpook province is estimated to be about 50,000, but only 20 percent out of them ws registered. The rehabilitation facilities located in this area receives 69% of their operational costs from central government. This figure is higher than the national average of 64.6%. 5. Although RSDS is different from other medical delivery system, nonetheless, it should be developed as a sub-system of national health care delivery system. Its development should also be in accordance with the need of local community. Therefore we suggest the development policy for the development of RMDS must consider the following points.; First, all rehabilitation-related medical systems should be integrated unde ra single comprehensive system so that handicapped persons may easily obtain better service available. Second, rehabilitation programs based on local community should be rejuvenated. In addition to in-patient service, development of intermediate facilities and programs connecting home and society should be encouraged. Third, rehabilitation medical services should be more specialized. Also, more emphasis should be given to team approach to rehabilitation medical services. Fourth, a comprehensive rehabilitation information system should be developed so that handicapped persons may have easy access to public welfare facilities, and the central government should formulate a comprehensive long-term plan for the rehabilitation system.

거대 난소 종양의 염전 1예

김미영,이해혁,유애리,박정희,이임순,정수호,고은석,레앙소팥,배동한,김태희,이권해 순천향의학연구소 2008 Journal of Soonchunhyang Medical Science Vol.14 No.1

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초등학교 학생들의 비례논리 전략의 발달에 대한 연구

정완호,권용주,김영신 한국교원대학교 과학교육연구소 1998 청람과학교육연구논총 Vol.8 No.1

The purpose of the present study was to investigate the development of elementary school student's reasoning strategies used in proportional tasks. Three hundred and ninety elementary students were sampled to investigate their reasoning strategies used in Pouring Water Tasks. Results showed that 4 percentage of students used proportional reasoning strategy. By the way, about 80% of students used qualitative guess or additive strategies to solve proportion tasks. Further, about fifth-grade or 11-year-old students began to use proportional reasoning strategy. Also, female and male students' development of reasoning strategies improved from 1st grade across 5th grade and from 6-year-old across 11-year-old. However, female did not show the improvement of strategy development after 5th-grade or 11-year-old. However, male students showed a continuous improvement after the grade or age. In addition, students showed developmental patterns of spurts and plateau, rather than a linear developmental pattern. The present study also discussed educational implications of this findings in school curriculum.

무용창작의 구성방법에 관한 연구

정은혜,권영옥 忠南大學校體育科學硏究所 1996 體育科學硏究誌 Vol.14 No.1

The creation of dance contains a variety of complexed factors. Space and time, the balancement of human body and the eventual harmony in space from the movement, the dynamic energy and the expression of the movement will form a creative dance. This study aims to find out a scientific method o composition in choreography by the theoritical evaluation of the concept of dance creation and by analyzing the esthentic definition of the dance; -Creating the dance will not merely 'making the dance', since the dance would have the mission of expression and conveying a theme. -A superb dancing art will commonly consist of the esthentic definition of unity, variety, repetition, contrast, transition, sequence, climax, balance and harmony. -Firm theme will be accompanied by spacial factor, tempo and dynamic energy. A correspondence will exist between the creator and the spectator provided the formality and definition of the composition is dissolved in creating work of dancing art.

벼의 잎 ·목 稻熱病에 대한 抵抗性의 比較

權寧閑,鄭厚燮,趙鏞涉,朴恩雨 서울大學校 農科大學 1986 서울대농학연구지 Vol.11 No.1

1. Differences in reactions of leaf and neck of rice plants to rice blast among four rice cultivars were observed in a paddy field. Cultivars Jinheung and Milyang 23 showed susceptible and resistant reactions, respectively, on both leaf and neck. Cultivar Norin 6 was susceptible to leaf blast and became intermediately resistant to neck blast whereas cultivar Ginga showed resistant and intermediately resistant reactions on leaf and neck, respectively. 2. Three different heading dates at 5 to 8 day intervals were induced by transplanting seedlings of four cultivars from seedling beds to a paddy field three times at 9 to 10 day intervals. When blast severity was compared within cultivars, leaf blast severity increased as heading dates became late. However, neck blast severity was greater in plots with early heading dates than those with late heading dates. 3. Spore germination of two isolates of Pyricularia oryzae in tissue extracts from leaf and neck of four rice cultivars indicated significant interactions between cultivars and isolates. However, spore germinations in leaf and neck extracts were not significantly different. Mean degree of hyphal growth of four P. oryzae isolates on the sheath adaxial epidermis indicated significant interactions between cultivars and isolates. 4. Some anatomical characteristics of leaf and neck of four cultivars did not have apparent relationship with the reactions of four cultivars to rice blast in the paddy field. Also, the amounts of the total nitrogen, sugar, P₂O??, and SiO in flag leaf and neck were not significantly different among four cultivars and did not seem to affect changes in reactions of cultivars Norin 6 and Ginga to leaf and neck blasts observed in the paddy field.

중학생들의 과학적 사고 수준과 교과서 생물 분야의 탐구활동에서 요구하는 사고수준의 분석

정완호,김영신,권용주 한국교원대학교 과학교육연구소 1999 청람과학교육연구논총 Vol.9 No.1

The purpose of this study was to analyze the relationship between junior high school students' reasoning level and thinking pattern of biology inquiry activities in their science textbook. Students' scientific reasoning abilities were investigated by the Lawson's Scientific Reasoning Group Test which was adminstered to 725 junior high school students. Four hundred and sixty-four inquiry activities excerpted from 8 science textbooks were analyzed by the profile of reasoning patterns which were composed of sub-categories of empirical-inductive reasoning and hypothetico-deductive reasoning patterns. The results of this study showed that 38.9 percentiles of all subjects were categorized as empirical-inductive reasoner and 29.1 percentiles were hypothetico-deductive reasoner. Although the percentile of hypothetico-deductive reasoner was increased from the 7th to the 8th grades, the result showed it was decreased from the 8th to the 9th grades which provided another positive evidence for Kwon's theory of the spurt-and-plateau pattern of reasoning development. In addition, the result showed that 86.4 percentiles of all inquiry activities were revealed as empirical-inductive reasoning pattern and 13.6 percentiles were turn in revealed as hypothetico-deductive reasoning pattern. Even the percentile of hypothetical-deductive reasoning pattern of inquiry activities was increased from the 7th to 9th grades, it was lower than the percentiles of students' hypothetico-deductive reasoning level.

조기 후두암의 Docetaxel-Cisplatin 유도 화학요법에 대한 임상연구

정주섭,권보란,최영진,왕수건,이병주,조군제 부산대학교 병원 암연구소 2009 부산대병원학술지 Vol.- No.25