Comparative transcriptome analysis to identify genes involved in terpenoid biosynthesis in Agriophyllum squarrosum, a folk medicinal herb native to Asian temperature deserts

Yin Xiaoyue,Yan-Xia Liu,Qian Chaoju,Zhou Shanshan,Fang Tingzhou,Fan Xingke,Gao yuan,Chang Yuxiao,Yang Jian,Ma Xiao-Fei 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.3

Agriophyllum squarrosum is a folk Mongolian medicine with pleiotropic pharmacological and ecological economic importance endemic to Asian temperature deserts. Terpenoids play critical roles in biotic and abiotic stresses due to their antioxidative activities. Based on non-targeted metabolomic analysis, we detected eight terpenoids enriched in the above-ground tissues of A. squarrosum, however, the molecular mechanism underlying terpenoids biosynthesis in this desert medicinal plant is rarely understood. Here, a comparative transcriptome analysis of diferent tissues in A. squarrosum was conducted to identify 84 unigenes encoding key enzymes in the upstream backbone biosynthesis and 53 unigenes encoding the downstream enzymes for terpenoid diversifcation. Most of the upstream genes exhibited signifcant high expression levels in leaf, and some of which were validated by qRT-PCR. Phylogenetic analysis showed that two downstream gene families OSCs (oxidosqualene cyclases) and TPSs (mainly in terpene synthases -g subfamily) had undergone notable gene expansions in A. squarrosum comparing with the other Amaranthaceae plant species and Arabidopsis. Nevertheless, most members from these two gene families showed the tissue-specifc expression in A. squarrosum, which supported the diversifcation and tissue-specifc enrichment of terpenoids across above-ground tissues. Considering to the habitat characteristics of A. squarrosum, we proposed that the enrichment of terpenoids and the functional diversifcation of terpenoids biosynthesis enzymes were more or less involved into its adaptation to stressful environments of deserts. These results expand the available genetic information underlying terpenoid biosynthesis in A. squarrosum, and contribute to deeper researches on pharmaceutical and eco-agricultural applications in this desert medicinal plant.

Computerized bone age estimation system based on China-05 standard

Yin, Chuangao,Zhang, Miao,Wang, Chang,Lin, Huihui,Li, Gengwu,Zhu, Lichun,Fei, Weimin,Wang, Xiaoyu Techno-Press 2022 Advances in nano research Vol.12 No.2

The purpose of this study is to develop an automatic software system for bone age evaluation and to evaluate its accuracy in testing and feasibility in clinical practice. 20394 left-hand radiographs of healthy children (2-18 years old) were collected from China Skeletal Development Survey data of 1998 and China Skeletal Development Survey data of 2005. Three experienced radiologists and China-05 standard maker jointly evaluate the stages of bone development and the reference bone age was determined by consensus. 1020 from 20394 radiographs were picked randomly as test set and the remaining 19374 radiographs as training set and validation set. Accuracy of the automatic software system for bone age assessment is evaluated in test set and two clinical test sets. Compared with the reference standard, the automatic software system based on RUS-CHN for bone age assessment has a 0.04 years old mean difference, ±0.40 years old in 95% confidence interval by single reading, a 85.6% percentage agreement of ratings, a 93.7% bone age accuracy rate, 0.17 years old of MAD, 0.29 years old of RMS; Compared with the reference standard, the automatic software system based on TW3-C RUS has a 0.04 years old mean difference, a ±0.38 years old in 95% confidence interval by single reading, a 90.9% percentage agreement of ratings, a 93.2% bone age accuracy rate, a 0.16 years of MAD, and a 0.28 years of RMS. Automatic software system, AI-China-05 showed reliably accuracy in bone age estimation and steady determination in different clinical test sets.

Proteomic analysis of chicken peripheral blood mononuclear cells after infection by Newcastle disease virus

Xiaoyu Deng,Yanlong Cong,Renfu Yin,Guilian Yang,Chan Ding,Shengqing Yu,Xiufan Liu,Chun-feng Wang,Zhuang Ding 대한수의학회 2014 JOURNAL OF VETERINARY SCIENCE Vol.15 No.4

Characteristic clinical manifestations of Newcastle diseaseinclude leukopenia and immunosuppression. Peripheral bloodmononuclear cells (PBMCs) are the main targets of Newcastledisease virus (NDV) infection. To survey changes in proteomicexpression in chicken PBMCs following NDV infection,PBMC proteins from 30 chickens were separated using twodimensionalelectrophoresis (2-DE) and subjected to massspectrometry analysis. Quantitative intensity analysis showedthat the expression of 78 proteins increased more thantwo-fold. Thirty-five proteins exhibited consistent changes inexpression and 13 were identified as unique proteins bymatrix assisted laser desorption ionization-time of flight massspectrometer/mass spectrometer including three that weredown-regulated and 10 that were up-regulated. These proteinswere sorted into five groups based on function: macromolecularbiosynthesis, cytoskeleton organization, metabolism, stressresponses, and signal transduction. Furthermore, Westernblot analysis confirmed the down-regulation of integrin-linkedkinase expression and up-regulation of lamin A production. These data provide insight into the in vivo response of targetcells to NDV infection at the molecular level. Additionally,results from this study have helped elucidate the molecularpathogenesis of NDV and may facilitate the development ofnew antiviral therapies as well as innovative diagnostic methods.

Acute Effects of Transforming Growth Factor-β1 on Neuronal Excitability and Involvement in the Pain of Rats with Chronic Pancreatitis

( Xiaoyu Zhang ),( Hang Zheng ),( Hong-yan Zhu ),( Shufen Hu ),( Shusheng Wang ),( Xinghong Jiang ),( Guang-yin Xu ) 대한소화기기능성질환·운동학회 2016 Journal of Neurogastroenterology and Motility (JNM Vol.22 No.2

Background/Aims This study was to investigate whether transforming growth factor-β1 (TGF-β1) plays a role in hyperalgesia in chronic pancreatitis (CP) and the underlying mechanisms. Methods CP was induced in male adult rats by intraductal injection of trinitrobenzene sulfonic acid (TNBS). Abdominal hyperalgesia was assessed by referred somatic behaviors to mechanical stimulation of rat abdomen. Dil dye injected into the pancreas was used to label pancreas-specific dorsal root ganglion (DRG) neurons. Whole cell patch clamp recordings and calcium imaging were performed to examine the effect of TGF-β1 on acutely isolated pancreas-specific DRG neurons. Western blot analysis was carried out to measure the expression of TGF-β1 and its receptors. Results TNBS injection significantly upregulated expression of TGF-β1 in the pancreas and DRGs, and TGF-β1 receptors in DRGs (T9-T13) in CP rats. Intrathecal injection of TGF-β receptor I antagonist SB431542 attenuated abdominal hyperalgesia in CP rats. TGF-β1 application depolarized the membrane potential and caused firing activity of DRG neurons. TGF-β1 application also reduced rheobase, hyperpolarized action potential threshold, and increased numbers of action potentials evoked by current injection of pancreas-specific DRG neurons. TGF-β1 application also increased the concentration of intracellular calcium of DRG neurons, which was inhibited by SB431542. Furthermore, intrathecal injection of TGF-β1 produced abdominal hyperalgesia in healthy rats. Conclusions These results suggest that TGF-β1 enhances neuronal excitability and increases the concentration of intracellular calcium. TGF-β1 and its receptors are involved in abdominal hyperalgesia in CP. This and future study might identify a potentially novel target for the treatment of abdominal pain in CP. (J Neurogastroenterol Motil 2016;22:333-343)

Porous NiS@Ni2P nanoframe as a multi-functional catalyst for enhanced oxygen evolution and urea oxidation reactions

Yin Huang,Yaoyao Pan,Xiaoyu Huang,Jialu Zhao,Xiuhua Wang 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.118 No.-

During water electrolysis, multifunctional electrocatalysts with outstanding functionality and endurancemust be thoughtfully constructed and designed. Below, we report a hollow NiS@Ni2P nanoframeheterostructure that works well as a substitute catalyst for the urea oxidation and general water splittingreactions. The hollow NiS@Ni2P nanoframe was synthesized by ion exchange, sulfurization and phosphating,which is favorable for achieving a heterostructure. The NiS@Ni2P catalyst exhibits excellent catalyticactivity and strong long-term stability in 1.0 M KOH solutions for the hydrogen evolution reaction, whichneeds 121 mV to achieve 10 mA cm2. And for the oxygen evolution reaction, the catalyst needs 311 mVto acquire 50 mA cm2. These advantages come from the optimal electronic structural configuration,hierarchical hollow nanoframe structure, and large surface area. It could attain 10 mA cm2 at 1.41 Vwhen utilized as a urea oxidation reaction anode, which is lower than the oxygen evolution reaction. For total water splitting and urea oxidation process, NiS@Ni2P as a bifunctional catalyst holds tremendouspromise due to its strong electrocatalytic activity, ease of manufacture, and low cost of raw ingredients.

Influence of Pore Structure Characteristics on the Mechanical and Durability Behavior of Pervious Concrete Material Based on Image Analysis

Ruyan Liu,Yin Chi,Shengyun Chen,Qinghui Jiang,Xiaoyu Meng,Kun Wu,Shengjia Li 한국콘크리트학회 2020 International Journal of Concrete Structures and M Vol.14 No.4

Pervious concrete has been widely used in parking lots and other lightweight streets. Performances of pervious concrete are strongly dependent on its pore structure characteristics. This paper investigates the relationship among porosity, permeability, compressive strength, durability and the pore structure characteristics of pervious concrete. The influences of basalt fiber and fine aggregate on the pervious concrete are also discussed. Pervious concrete specimens with different pore structures were prepared by combining basalt aggregate with size of 5-10 mm and 10-15 mm at different proportion. The pore structure characteristics such as area porosity, pore sizes, and pore distribution of pervious concrete were obtained and analyzed using image analysis method. Experimental results showed that the pore structure characteristics of pervious concrete have effects on its mechanical and durability behaviors. Homogeneous pore distribution and large distances between pores increase the compressive strength and improve freeze-thaw durability of pervious concrete. Due to the difference in the internal pore structure of pervious concretes, specimens with similar volumetric porosities had different permeability coefficients. A series of numerical models were regenerated through a MATLAB code using pore structural parameters derived from the image analysis method, then the numerical models were fed to ANSYS for meshing and further simulation. The comparison of the simulation and experimental results showed close accuracy, which verified the applied simulation analysis method based on image analysis technology.

Controlled self-assembly of low-dimensional Alq3 nanostructures from 1D nanowires to 2D plates via intermolecular interactions

Jianmin Gu,Baipeng Yin,Shaoyan Fu,Cuihong Jin,Xin Liu,Zhenpan Bian,Jianjun Li,Lu Wang,Xiaoyu Li 대한금속·재료학회 2018 ELECTRONIC MATERIALS LETTERS Vol.14 No.2

Due to the intense influence of the shape and size of the photon building blocks on the limitation and guidance of opticalwaves, an important strategy is the fabrication of different structures. Herein, organic semiconductor tris-(8-hydroxyquinoline)aluminium (Alq3) nanostructures with controllable morphology, ranging from one-dimensional nanowires to twodimensionalplates, have been prepared through altering intermolecular interactions with employing the anti-solvent diffusioncooperate with solvent-volatilization induced self-assembly method. The morphologies of the formed nanostructures, whichare closely related to the stacking modes of the molecules, can be exactly controlled by altering the polarity of anti-solventsthat can influence various intermolecular interactions. The synthesis strategy reported here can potentially be extended toother functional organic nanomaterials.

Implementation of the Fuzzy C-Means Clustering Algorithm in Meteorological Data

Yinghua Lu,Tinghuai Ma,Changhong Yin,Xiaoyu Xie,Wei Tian,ShuiMing Zhong 보안공학연구지원센터 2013 International Journal of Database Theory and Appli Vol.6 No.6

Heterologous prime-boost immunization with live SPY1 and DnaJ protein of Streptococcus pneumoniae induces strong Th1 and Th17 cellular immune responses in mice

Yulan Qiu,XueMei Zhang,Hong Wang,Xinyuan Zhang,Yunjun Mo,Xiaoyu Sun,Jichao Wang,YiBing Yin,WenChun Xu 한국미생물학회 2017 The journal of microbiology Vol.55 No.10

diseases in children under 5-year-old. Vaccine has been used as an indispensable strategy to prevent S. pneumoniae infection for more than 30 years. Our previous studies confirmed that mucosal immunization with live attenuated strain SPY1 can protect mice against nasopharyngeal colonization of S. pneumoniae and lethal pneumococcal infection, and the protective effects are comparable with those induced by commercially available 23-valent polysaccharide vaccine. However, live attenuated vaccine SPY1 needs four inoculations to get satisfactory protective effect, which may increase the risk of virulence recovery. It is reported that heterologous primeboost approach is more effective than homologous primeboost approach. In the present study, to decrease the doses of live SPY1 and improve the safety of SPY1 vaccine, we immunized mice with SPY1 and DnaJ protein alternately. Our results showed that heterologous prime-boost immunization with SPY1 and DnaJ protein could significantly reduce the colonization of S. pneumoniae in the respiratory tract of mice, and induce stronger Th1 and Th17 cellular immune responses than SPY1 alone. These results indicate heterologous prime-boost immunization method not only elicits better protective effect than SPY1 alone, but also reduces the doses of live SPY1 and decreases the risk of SPY1 vaccine. This work is the first time to study the protective efficiency with two different forms of S. pneumoniae candidate vaccine, and provides a new strategy for the development of S. pneumoniae vaccine.

Molecular characterization of duck enteritis virus CHv strain UL49.5 protein and its colocalization with glycoprotein M

Meng Lin,Renyong Jia,Mingshu Wang,Xinghong Gao,Dekang Zhu,Shun Chen,Mafeng Liu,Zhongqiong Yin,Yin Wang,Xiaoyue Chen,Anchun Cheng 대한수의학회 2014 JOURNAL OF VETERINARY SCIENCE Vol.15 No.3

The UL49.5 gene of most herpesviruses is conserved andencodes glycoprotein N. However, the UL49.5 protein ofduck enteritis virus (DEV) (pUL49.5) has not been reported. In the current study, the DEV pUL49.5 gene was firstsubjected to molecular characterization. To verify thepredicted intracellular localization of gene expression, therecombinant plasmid pEGFP-C1/pUL49.5 was constructedand used to transfect duck embryo fibroblasts. Next, therecombinant plasmid pDsRed1-N1/ glycoprotein M (gM)was produced and used for co-transfection with thepEGFP-C1/pUL49.5 plasmid to determine whether DEVpUL49.5 and gM (a conserved protein in herpesviruses)colocalize. DEV pUL49.5 was thought to be an envelopeglycoprotein with a signal peptide and two transmembranedomains. This protein was also predicted to localize in thecytoplasm and endoplasmic reticulum with a probability of66.7%. Images taken by a fluorescence microscope atdifferent time points revealed that the DEV pUL49.5 andgM proteins were both expressed in the cytoplasm. Overlapof the two different fluorescence signals appeared 12 h aftertransfection and continued to persist until the end of theexperiment. These data indicate a possible interaction between DEV pUL49.5 and gM.