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Optimization of Medium Components for Plasmid Production by Recombinant E. coli DH5α pUK21CMVβ1.2
Yinghua Lu,Shuzhen Zheng,Karl Friehs,Ning He,Xu Deng,Qingbiao Li,Zheng He,Chenyun Xu 한국생물공학회 2007 Biotechnology and Bioprocess Engineering Vol.12 No.3
In this study we investigated the effects of carbon and nitrogen sources, and the carbon to nitrogen ratio (C:N) of growth medium on the production of plasmid DNA by pUK21CMVβ1.2 harbouring recombinant E. coli. The results indicated that sucrose and casein peptone were the most suitable carbon and nitrogen sources, respectively, for plasmid production, and the plasmid concentration was significantly influenced by the C:N ratio. A Box-Behnken design and surface response methodology were employed to formulate the optimal medium. In the optimized medium, a plasmid concentration of 51.80 mg/L and a plasmid selectivity of 23.49 mg/g were achieved after 24 h of incubation. These values were approximately 3.5 and 1.7 times higher, respectively, than those obtained on the original LB medium.
余颖华(Yinghua Yu),周蕗(Lu Zhou) YIXIN 출판사 2024 Journal of China Studies Vol.2 No.3
本研究以江西的鹅湖书院为例,探讨了书院文化负载词的英译问题。书院作为中国传统文化的重要载体,蕴含了丰富的历史、哲学、教育和地方特色等元素,这些元素在英译过程中往往面临诸多挑战。首先对书院文化负载词进行了分类并结合翻译理论和实践探讨了文化负载词的翻译策略,包括直译、 意译、释义等方法,旨在保持原文文化内涵的同时,确保译文的准确性和可读性。 This study takes Goose Lake Academy in Jiangxi Province as an example to explore the English translation of culture-loaded words in the academy. As an important carrier of Chinese traditional culture, academies contain rich elements of history, philosophy, education and local characteristics, which often face many challenges in the process of English translation. Combining translation theory and practice, this study explores the translation strategies of culture-loaded words, including literal translation, free translation, paraphrased and other methods, in order to maintain the cultural connotation of the original text and ensure the accuracy and readability of the translation.
I-Son Ng,Xuesong Zheng,Bor-Yann Chen,Xiaoqin Chi,Yinghua Lu,Chun-Sheng Chang 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.1
The first-attempt study employed a proteomics strategy for the identification of abundant proteins from a bioelectricity generation and dye decolorization bacterium Proteus hauseri ZMd44. By using the degenerated primers designed based on the peptide sequences from tandem mass spectroscopy and the whole genomics annotation of the closely associated strain, Proteus penneri ATCC 35198,the genes were successfully obtained for two full-length genes of 543 bp (laccase) and 1,086 bp (Omp F, porin)encoding to 181 amino acids and 362 amino acids,respectively. It explored laccase and NADH dehydrogenase involvement in the oxidation-reduction reaction as well, as porin played an important role in providing channels for related proteins in the accomplishment of electron transportation in P. hauseri. Detailed enzymatic assays indicated that laccase activity of 542.2 U/DCW could be stimulated by 2.5 mM copper induction in LB medium (ca. 293-fold to those without copper induction). Among intracellular proteins, NADH dehydrogenase activity of 257.2 U/mg via mediator riboflavin was in parallel with the decolorizing capability of azo dye Rb160 that only took place in LB medium. From the evaluation of kinetic parameters (Vmax and Km were 0.272 U/min and 0.393 mM with ABTS,0.046 U/min and 43.8 μM with NADH), it is better to decipher the decolorization mechanism of ZMd44 indicating that laccase and NADH dehydrogenase played the most crucial role for azo dye decolorization.
Zheng, Shuzhen,Friehs, Karl,He, Ning,Deng, Xu,Li, Qingbiao,He, Zheng,Xu, Chenyun,Lu, Yinghua Korean Society for Biotechnology and Bioengineerin 2007 Biotechnology and Bioprocess Engineering Vol.12 No.3
In this study we investigated the effects of carbon and nitrogen sources, and the carbon to nitrogen ratio (C:N) of growth medium on the production of plasmid DNA by $pUK21CMV{\beta}1.2$ harbouring recombinant E. coli. The results indicated that sucrose and casein peptone were the most suitable carbon and nitrogen sources, respectively, for plasmid production, and the plasmid concentration was significantly influenced by the C:N ratio. A Box-Behnken design and surface response methodology were employed to formulate the optimal medium. In the optimized medium, a plasmid concentration of 51.80mg/L and a plasmid selectivity of 23.49mg/g were achieved after 24h of incubation. These values were approximately 3.5 and 1.7 times higher, respectively, than those obtained on the original LB medium.