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Xiaoyu Deng,Yanlong Cong,Renfu Yin,Guilian Yang,Chan Ding,Shengqing Yu,Xiufan Liu,Chun-feng Wang,Zhuang Ding 대한수의학회 2014 JOURNAL OF VETERINARY SCIENCE Vol.15 No.4
Characteristic clinical manifestations of Newcastle diseaseinclude leukopenia and immunosuppression. Peripheral bloodmononuclear cells (PBMCs) are the main targets of Newcastledisease virus (NDV) infection. To survey changes in proteomicexpression in chicken PBMCs following NDV infection,PBMC proteins from 30 chickens were separated using twodimensionalelectrophoresis (2-DE) and subjected to massspectrometry analysis. Quantitative intensity analysis showedthat the expression of 78 proteins increased more thantwo-fold. Thirty-five proteins exhibited consistent changes inexpression and 13 were identified as unique proteins bymatrix assisted laser desorption ionization-time of flight massspectrometer/mass spectrometer including three that weredown-regulated and 10 that were up-regulated. These proteinswere sorted into five groups based on function: macromolecularbiosynthesis, cytoskeleton organization, metabolism, stressresponses, and signal transduction. Furthermore, Westernblot analysis confirmed the down-regulation of integrin-linkedkinase expression and up-regulation of lamin A production. These data provide insight into the in vivo response of targetcells to NDV infection at the molecular level. Additionally,results from this study have helped elucidate the molecularpathogenesis of NDV and may facilitate the development ofnew antiviral therapies as well as innovative diagnostic methods.
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Yingjie Sun,Pin Zhang,Hang Zheng,Luna Dong,Lei Tan,Cuiping Song,Xusheng Qiu,Ying Liao,Chunchun Meng,Shengqing Yu,Chan Ding 대한수의학회 2018 Journal of Veterinary Science Vol.19 No.1
T-cell internal antigen-1 (TIA-1) has roles in regulating alternative pre-mRNA splicing, mRNA translation, and stress granule (SG) formation in human cells. As an evolutionarily conserved response to environmental stress, SGs have been reported in various species. However, SG formation in chicken cells and the role of chicken TIA-1 (cTIA-1) in SG assembly has not been elucidated. In the present study, we cloned cTIA-1 and showed that it facilitates the assembly of canonical SGs in both human and chicken cells. Overexpression of the chicken prion-related domain (cPRD) of cTIA-1 that bore an N-terminal green fluorescent protein (GFP) tag (pntGFP-cPRD) or Flag tag (pFlag-cPRD) induced the production of typical SGs. However, C-terminal GFP-tagged cPRD induced notably large cytoplasmic granules that were devoid of endogenous G3BP1 and remained stable when exposed to cycloheximide, indicating that these were not typical SGs, and that the pntGFP tag influences cPRD localization. Finally, endogenous cTIA-1 was recruited to SGs in chicken cells and tissues under environmental stress. Taken together, our study provide evidence that cTIA-1 has a role in canonical SG formation in chicken cells and tissues. Our results also indicate that cPRD is necessary for SG aggregation.
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