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RISS 인기검색어
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- 저자 : Mingshu Wang (검색결과 5 건)
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Apoptosis induced in vivo by new type gosling viral enteritis virus
Shun Chen,Anchun Cheng,Mingshu Wang,Dekang Zhu,Renyong Jia,Qihui Luo,Hengmin Cui,Yi Zhou,Yin Wang,Zhiwen Xu,Zhengli Chen,Xiaoyue Chen,Xiaoyu Wang 대한수의학회 2011 JOURNAL OF VETERINARY SCIENCE Vol.12 No.4
In this study, apoptosis was induced by new type gosling viral enteritis virus (NGVEV) in experimentally infected goslings is reported in detail for the first time. After 3-day-old goslings were orally inoculated with a NGVEV-CN strain suspension, the time course of NGVEV effects on apoptotic morphological changes of the internal tissues was evaluated. These changes were observed by histological analysis with light microscopy and ultrastructural analysis with transmission electron microscopy. DNA fragmentation was assessed with a terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay and DNA ladder analysis. A series of characteristic apoptotic morphological changes including chromatin condensation and margination, cytoplasmic shrinkage, plasma membrane blebbing, and formation of apoptotic bodies were noted. Apoptosis was readily observed in the lymphoid and gastrointestinal organs, and sporadically occurred in other organs after 3 days post-infection (PI). The presence and quantity of TUNEL-positive cells increased with infection time until 9 days PI. DNA extracted from the NGVEV-infected gosling cells displayed characteristic 180~200 bp ladders. Apoptotic cells were ubiquitously distributed, especially among lymphocytes, macrophages, monocytes, and epithelial and intestinal cells. Necrosis was subsequently detected during the late NGVEV-infection phase, which was characterized by cell swelling, plasma membrane collapse, and rapidly lysis. Our results suggested that apoptosis may play an important role in the pathogenesis of NGVE disease.
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A RFID Data-Cleaning Algorithm Based on Communication Information among RFID Readers
Yunhua Gu,Bao Gao,Jin Wang,Mingshu Yin,Junyong Zhang 보안공학연구지원센터(IJUNESST) 2015 International Journal of u- and e- Service, Scienc Vol.8 No.1
For the insufficient of existing algorithms of redundant-reader elimination, the paper proposes a data-cleaning algorithm based on communication information among readers. This algorithm is no longer dependent on the tag to send information between the RFID readers, but to transmit information directly through communication information among the readers to determine which reader the tag belongs to and whether the reader is redundant or not. This paper does a lot of experiments to verify the effectiveness of the algorithm.
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Fixed-time Disturbance Observer-based Sliding Mode Control for Mismatched Uncertain Systems
Yang Wang,Mingshu Chen 제어·로봇·시스템학회 2022 International Journal of Control, Automation, and Vol.20 No.9
This paper concerns with fixed-time control for the mismatched uncertain system. We propose a fixedtime disturbance observer (DO). Then, a non-singular fixed-time sliding-mode surface and controller are designed based on the estimations of proposed DO. Existing DO-based sliding-mode control (DOSMC) and finite-time DOSMC schemes can eliminate the mismatched uncertain problem by using traditional sliding-mode control (TSMC). However, the convergence times of these DOSMC schemes are infinite or related to the initial system conditions. Unlike existing approaches, the proposed fixed-time DOSMC can guarantee the convergence time is uniformly bounded and the upper bound is independent on initial system conditions, which implies that the proposed scheme can provide similar convergence performance with the same control parameters under different initial conditions. Simulation result shows the excellent convergence performance of the proposed scheme.
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Meng Lin,Renyong Jia,Mingshu Wang,Xinghong Gao,Dekang Zhu,Shun Chen,Mafeng Liu,Zhongqiong Yin,Yin Wang,Xiaoyue Chen,Anchun Cheng 대한수의학회 2014 JOURNAL OF VETERINARY SCIENCE Vol.15 No.3
The UL49.5 gene of most herpesviruses is conserved andencodes glycoprotein N. However, the UL49.5 protein ofduck enteritis virus (DEV) (pUL49.5) has not been reported. In the current study, the DEV pUL49.5 gene was firstsubjected to molecular characterization. To verify thepredicted intracellular localization of gene expression, therecombinant plasmid pEGFP-C1/pUL49.5 was constructedand used to transfect duck embryo fibroblasts. Next, therecombinant plasmid pDsRed1-N1/ glycoprotein M (gM)was produced and used for co-transfection with thepEGFP-C1/pUL49.5 plasmid to determine whether DEVpUL49.5 and gM (a conserved protein in herpesviruses)colocalize. DEV pUL49.5 was thought to be an envelopeglycoprotein with a signal peptide and two transmembranedomains. This protein was also predicted to localize in thecytoplasm and endoplasmic reticulum with a probability of66.7%. Images taken by a fluorescence microscope atdifferent time points revealed that the DEV pUL49.5 andgM proteins were both expressed in the cytoplasm. Overlapof the two different fluorescence signals appeared 12 h aftertransfection and continued to persist until the end of theexperiment. These data indicate a possible interaction between DEV pUL49.5 and gM.
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Najeeb ur Rehman Lashari,Mingshu Zhao,Jun Wang,XINHAI HE,Irfan Ahmed,Miao Miao Liang,Songpon Tangsee,Xiaoping Song 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.108 No.-
The energy storage properties of layered metal vanadate, especially alkali metal vanadates have beenextensively studied. Metal vanadates have a more robust electrochemical output in contrast with pristinevanadium oxides. However, the detailed processes underlying the efficiency contrast of vanadates andvanadium oxides have rarely been studied. Herein a facile hydrothermal and low-temperature polymerizationmethod was introduced to synthesize KV3O8 and KV3O8@PPy nanowire bundles as anode materialfor an aqueous rechargeable Lithium batteries. The nanowires are composed of KV3O8·0.59H2O calculatedusing thermal gravimetric analysis (TGA). Successfully synthesized layered vanadium based KV3O80.59H2O (KVO) and KV3O8 0.59H2O@PPy (KVO@PPy) nanowires and investigated the source of theimproved electrochemical efficiency of PPy coated potassium vanadates compared to pristine KVO usingcrystal structure analysis and electrochemical tests. We demonstrated increase in electrochemical stabilityfor KVO@PPy caused by synergistic effect of K+ in vanadate nanowires and PPy coating. In KVO theoxygen atoms have close contact with the K ions, and the stable K+ serve as ‘‘pillars” between interlayersto shield the layered structures from collapse during the charge/discharge phase, while the PPy reducescharge transfer resistance. This research adds helps us design better electrode materials to be used as ananode material for ARLB using alkali metal vanadate.
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