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류마티스 활막염에 있어 염증매개물질에 의한 Transforming Growth Factor-β-inducible Gene-h3 (βig-h3) 생산 조절 기전
강영모 ( Young Mo Kang ),김성일 ( Sung Il Kim ),김정섭 ( Jeong Seup Kim ),유동완 ( Dong Wan You ),사금희 ( Kheum Hee Sa ),박은주 ( Eun Ju Park ),김성욱 ( Sung Uk Kim ),서재석 ( Jae Seok Seo ),한승우 ( Seung Woo Han ),남언정 ( Eon 대한류마티스학회 2005 대한류마티스학회지 Vol.12 No.2
Objective: To investigate the expression pattern of transforming growth factor-β-inducible gene-h3 (βig-h3) within rheumatoid synovial tissue and the regulation of βig-h3 synthesis in fibroblast-like synoviocyte (FLS). Methods: Synovial tissues obtained from patients with rheumatoid arthritis and osteoarthritis were obtained during joint replacement surgery. βig-h3 expression was evaluated with immunohistochemical stain. FLS was isolated from synovial tissues and stimulated with cytokines including TGF-β, TNF-α, IL-1β, IFN-γ, IL-6, IL-4, and IL-10. βig-h3 synthesis was measured using semiquantitative RT-PCR, ELISA, immunofluorescence stain, and flow cytometry. Results: Expression of βig-h3 was diffuse and abundant in both lining and sublining layers of rheumatoid synovium, which was more prominent than those of osteoarthritis. Production of βig-h3 in FLS was regulated by TGF-β1 in a dose-dependent manner and was highest at 5 ng/mL of TGF-β1. TNF-α and IL-1β upregulated the production of βig-h3 from FLS synergistically with TGF-β1 but other cytokines such as IL-4, IL-6, IL-10 did not affect. βig-h3 synthesis was efficiently inhibited by dexamethasone at higher dose (100 nM) but not by cyclosporine-A. Conclusion: Production of βig-h3, which is highly upregulated in rheumatoid synovitis, is differentially regulated by inflammatory cytokines.
건선 모사 피부 염증모델에서 대황목단피탕(大黃牧丹皮湯)의 효능 연구
노경란,권빛나,김동욱,오진영,양갑식,조일주,Gyeong-Ran Noh,Bitna Kweon,Dong-Uk Kim,Jin‑Young Oh,Gabsik Yang,Il-Joo Jo 대한융합한의학회 2024 대한융합한의학회지 Vol.6 No.1
Objectives: Psoriasis is a common chronic inflammatory skin disease characterized by keratinocyte hyperproliferation and an excessive inflammatory response. Agents that can attenuate keratinocyte hyperproliferation and excessive inflammatory responses are considered potentially useful for the treatment of psoriasis. Daehwangmokdanpitang (DHMDPT) exhibits a broad range of bioactivities, including anti-proliferative and anti-inflammatory effects. This study aims to evaluate the anti-psoriatic potential of DHMDPT in vitro. Methods: HaCaT keratinocytes were stimulated with a mixture of IL-17A, IL-22, oncostatin M, IL-1α, and TNF-α (M5) to establish an in vitro psoriatic keratinocyte model. Cell viability was measured using the MTT assay. Quantitative real-time PCR (qRT-PCR) was performed to measure the mRNA levels of the hyperproliferative marker gene keratin 6 (KRT6) and inflammatory factors such as IL-6, TNF-α, and IL-23A. Additionally, chemokines including CCL5, CCL2, CCL20, and CXCL1 were measured by qRT-PCR. Results: DHMDPT attenuated M5-induced hyperproliferation, as indicated by a reduction in KRT6 expression in HaCaT keratinocytes. M5 stimulation significantly upregulated the mRNA levels of IL-6, TNF-α, and IL-23A. However, DHMDPT treatment attenuated the upregulation of IL-6 but not TNF-α or IL-23A. Additionally, DHMDPT inhibited the expression of CCL5, CCL2, and CXCL1, but not CCL20. Conclusion: DHMDPT effectively attenuated the M5-induced proliferation and inflammatory response in HaCaT keratinocytes. Therefore, DHMDPT could be an attractive candidate for future development as an anti-psoriatic agent.
Circulating IL-4 Levels in Lupus nephritis is increased and downregulated by cyclophosphamide
김성일 ( Sung Il Kim ),조미라 ( Mi La Cho ),이신석 ( Shin Seok Lee ),주영실 ( Yeong Shil Joo ),김완욱 ( Wan Uk Kim ),민준기 ( Jun Ki Min ),홍연식 ( Yeon Sik Hong ),이상헌 ( Sang Heon Lee ),박성환 ( Sung Hwan Park ),조철수 ( Chul S 대한내과학회 1998 대한내과학회 추계학술발표논문집 Vol.1998 No.1
그람양성구균에 대한 Teicoplanin과 Vancomycin의 시험관내 항균력
최태열,김경숙,전용관,서일혜,김정욱,이웅수,안정열,김홍석,정재용,최효선,김덕언,유진우 대한감염학회 1994 감염 Vol.26 No.1
An increasing frequency of methicillin resistant S. aureus(MRSA), methicillin resistant coagulase negative staphylococci(MRCNS) and Enterococcal infection have been observed in recent years. Teicoplanin is a new glycopeptide antibiotic obstained from the Actinoplanes teicomycetius. The molecular structure and spectrum of antimicrobial activity of teicoplanin is simillar to those of vancomycin, and has been reported to have an excellent in vitro and in vivo effect against various gram-positive infections. Therefore, we evaluated the in vitor susceptibility of gram positive cocci, such as, S. aureus, coagulase negative Staphylococci(CNS), and Enterococci to teicoplanin and vancomycin. The total 253 strains consisted of MSSA(40), MRSA(53), MSCNS(47), MRCNS(48), and Enterococci(65). They were assayed by disc diffusion and agar dilution. During the study, 57% of S. aureus and 49% of CNS showed resistance to methicillin. The inhibitory diameter of teicoplanin was 15-20mm in MSSA, 12-19mm in MRSA, 13-24mm in MSCNS, 11-23mm in MRCNS, and 15-22mm in Enterococci respectively, and showed sensitivity in all but 8 strains(3.2%). The range of the minimum inhibitory concentration (MIC) of teicoplanin to MSSA, MRSA, MSCNS, MRCNS and Enterococci were 9.12-2.0㎍/ml, 0.25-2.0㎍/ml, & 0.25-32㎍/ml, 0.12-1.0㎍/ml respectively. One case of S. haemolyticus was resistant to teicoplanin (32㎍/ml) by the agar dilution method. Eight minor (3.2%) and one major(0.4%) error was observed when the MIC and disk diffusion data were correlated with teicoplanin. As for vancomycin the inhibitory diameter was 17-21mm in MSSA, 15-21mm in MRSA, 18-26mm in MSCNS, 18-25mm in MRCNS, and 16-22mm in Enterococci respectively. The range of the MIC of vancomycin to MSSA, MRSA, MSCNS, MRCNS, and Enterococci were 0.25-1.0㎍/ml, 0.25-4.0㎍/ml, 0.5-2.0㎍/ml and 0.5-2.0㎍/ml respectively. One minor error (0.4%) was seen with the vancomycin disk. The MIC90 of MSSA and MRSA exhibited the same results in teicoplanin (1.0㎍/ml, 1.0㎍/ml), and vancomycin(2.0㎍/ml, 2.0㎍/ml). MSCNS and MRCNS exhibited greater MIC90 with teicoplanin(4.0㎍/ml, 8.0㎍/ml) than vancomycin(2.0㎍/ml, 2.0㎍/ml). Incontrase Enterococci were more susceptible to teicoplanin(0.5㎍/ml) than to vancomucin (2.0㎍/ml). Results from this analysis indicated that both teicoplanin and vancomycin were very excellent for gram positive infections, especially those resistant to methicillin.
Tryptophan Negatively Regulates IgE-mediated Mast Cell Activation
Prashanta Silwal(실왈 프라산타),Keuna Shin(신근아),Seulgi Choi(최슬기),Uk Namgung(남궁욱),Chan Yong Lee(이찬용),Jun-Young Heo(허준영),Kyu Lim(임규),Jong IL Park(박종일),Ki-Hwan Kim(김기환),Seung-Kiel Park(박승길) 대한체질인류학회 2017 해부·생물인류학 (Anat Biol Anthropol) Vol.30 No.2
비만세포는 알레르기 반응을 일으키는 주된 세포로서 항원 자극에 반응하여 알레르기 유발 물질인 히스타민, 단백질 분해효소, 염증성 지질 물질 및 사이토카인 등을 분비한다. 아미노산인 트립토판은 염증반응을 조절한다. 트립토판 투여는 비만세포가 관여하는 염증성 장염 증상을 완화시킨다. 그러나 트립토판이 비만세포의 알레르기 반응에 미치는 영향에 대한 연구는 없다. 본 저자들은 트립토판이 IgE 매개 알레르기 반응에 미치는 영향을 비만세포 수준에서 그리고 실험동물 생쥐에서 연구하였다. IgE-매개 수동 피부 아나필락시스를 생쥐에서 연구하였다. 또한 IgE-매개 비만세포 활성화 반응 즉, 탈과립 반응, 염증성 지질인 LTB4와 사토카인 (TNF-α와 IL-4) 등의 분비를 측정하였다. 트립토판을 생쥐에 복강 주사하면 IgE 매개 수동 피부 아나필락시스를 억제하였다. 또한 비만세포 수준에서도 트립토판은 IgE 매개 알레르기 반응들, 즉 탈과립 반응과 염증성 지질인 LTB4 및 사이토카인인 TNF-α와 IL4의 분비를 억제하였다. 이러한 결과로부터 트립토판은 IgE 매개 알레르기 반응을 세포 수준 및 실험동물 수준에서 억제함을 알 수 있었다. Mast cells are major immune cells in allergy to secrete allergic mediators by a degranulation process and make and secrete inflammatory lipids and cytokines in response to antigen stimulation. An amino acid tryptophan regulates immune functions. Tryptophan ameliorates inflammatory colitis in which mast cells are engaged. However, its effects on mast cells remain to be solved. We investigated the effect of tryptophan on IgE-mediated allergic responses in the mast cells and mice. IgEmediated passive cutaneous anaphylaxis (PCA) in mice were examined. Also IgE-mediated mast cell activation responses such as degranulation of stored granules and secretion of inflammatory lipid LTB4 and cytokines (TNF-α and IL-4) were measured. Intraperitoneal administration of tryptophan suppressed PCA in mice. Also, in the cellular level tryptophan inhibited IgE-mediated mast cell activation such as IgE-mediated degranulation and the production of LTB4. Also, it inhibited production of inflammatory cytokines TNF-α and IL-4. In summary, tryptophan suppressed IgE-mediated allergic activation in vivo and in vitro. Tryptophan supplementation is beneficial for IgE-mediated allergy.
金億煥,趙誠一 建國大學校 敎育硏究所 1982 論文集 Vol.6 No.-
In Piagetian scheme, children's classification behavior plays a significant role in the development of logical thinking, and classification ability develops when children understand that instances (objects, events and persons) are multi-dimensional. Furthermore, attributes can be used as bases for forming classifications. Children should be shown that single attributes can also be combined to form new sub-classes. That is, they must be able to co-ordinate two or more attributes. In connection with this ability children must also develop intellectual operations called reversibility and reciprocity. At last, this paper demonstrated a teaching strategy for social studies through the use of discovery ptocedures in developing classification ability of children.
리튬 이온 이차전지를 위한 부극용 Si 특성에 관한 연구
김성호,이상헌,박일현,김민철,우태욱,손영국 부산대학교 생산기술연구소 2000 生産技術硏究所論文集 Vol.59 No.-
리튬이온이차전지의 부극으로 Si를 사용하였다. 부극용 Si전극을 1회 충방전 시 Li이온이 탈 삽입될 때 각각의 전압에서 XRD 회절분석을 통하여 Li이온이 이동하여 Si와 반응하는 것을 조사하였다. 순환전압전류 및 충방전 시험을 통하여 전기 화학적 특성을 측정해본 결과 부극으로써 Si의 산화환원 반응이 양호하게 이루어졌으며 충방전에 대한 cycle number가 증가할수록 비가역적인 산화환원반응 및 부피변화로 인해 싸이클 특성 감소함을 나타내었다. Si with anode is used Li ion secondary batteries During Li ion intercalating at first cycle, XRD analysis studies at others voltages showed that Li ions are moved and reacted to Si The electrochemical properties of Si anode materials were evaluated by cyclic voltammetry and charge/discharge test. With cycle number increasing, cyclic characteristics are decreased for irreversible reduction/oxidation and volume change.
최소자승법을 이용한 다수 베타 방출 핵종 혼합물의 방사능 분석
선광일,남욱원,공경남,김창규,이동명,이상국 대한방사선 방어학회 2001 방사선방어학회지 Vol.26 No.4
베타선 스펙트럼의 최대 에너지가 확실하게 구별되는 2개의 핵종만을 포함하는 혼합시료의 경우에는 최대 에너지가 다르다는 점을 이용하여 손쉽게 각 핵종의 방사능값을 측정할 수 있다. 그러나 3개 이상의 베타 방출 핵종이 포함된 혼합물에 대해서는 각 핵종의 스펙트럼이 서로 겹치기 때문에 이러한 방법으로 구해진 방사능값은 신뢰도가 떨어지게 된다. 따라서, 본 연구에서는 최소자승법을 이용하여 혼합물의 중첩된 베타선 스펙트럼을 각각 분리 정량분석할 수 있는 밥법을 제시하였다. 또한, 실제로 4개의 베타 방출 핵종 ^3H, ^14C, ^16Cl, 90Sr)이 혼합된 사료를 조제하여 본 분석법을 검증한 결과 최고치 Reference value)와 분석치가 7% 이내에서 잘 일치함을 보였다. It is possible to count and perform quench correction on two β-label samples so long as the maximum β-energies are sufficiently different. However, when the coventional technique is applied to the radioassay of a mixture of more than three nuclides, the reliability of the activiteis determined is considerably reduced, resulting from the large overlapping of liquid scintillation pulse height distributions of ezch nuclide. A technique that allows the activities of multiple β-labeled samples to be radioassayed was proposed by using the least square method. The technique was applied to mixture samples of ^3H, ^14C, ^16Cl, and ^90Sr. The analytical values were in good agreement with the reference values within 7% relstive error.
새마을敎育의 效果的 推進方案을 위한 덴마아크 國民敎育의 比較硏究
柳泰永,金億煥,金忠起,趙誠一 건국대학교 1980 새마을연구논문집 Vol.1 No.-
The purpose of this study was to investigate the opinions of selected members of teaching staffs and administrator concerning the effective method to pursue the Saemaul Movement throughout the country. A sample of 82 educational institutions was used to draw the process of Saemaul education. The major scope of this study is as follows : ① Educational facilities and institutions ② Man power and teaching staff ③ Curriculum ④ Finance Responses of the teaching staff to the opinionnaire were collected and analized in terms of percentages for each of twenty one statements. A return of 59.5 percent by the respondents was collected by mail. An analysis of the information resulting from this study led to the following conclusions : 1.A Saemaul education training institute should be a training center for leaching staff members and faculties. 2.A contribution to the Denmark reconstruction due to the erect of social education. 3.The education development of national spirit should be studied by general public school and during the summer vacation. 4.Curriculum should be organized to enlighten the security of the nation, patirotism, and value system. 5.The foundation of social education should start from the Saemaul education. The important direction of Saemaul education should be organized as follows : 1.A Saemaul education should stress the spiritual education for insuring substantiality of the national development and public livehood. 2.A Saemaul education should be organized and emphasized the harmony and unification of the nation. Consequently, the comprehensive institution of Saemaul Movement should establish to pursue the effectiveness of them.