A Study on the Functionality of Halophytes from Coast area of Jeollanam-do

Tae-Man Ha,Yang-Joon An,Mi-Yeong Shin,Sook Park,Hak-Jae Park,Hyo-Jeong Kang,Su-Jeong Choi,Jae-Yeong Yu,Ji-Yun Jeon,Gyu-Yeon Oh 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

This study was undertaken to improve the perceived value of halophytes and contribute to the activity of the local economy by analyzing the antioxidant activity and mineral content of Suaeda japonica, Tetragonia tetragonoides, and Spergularia marina that grow wild on the mud flats and coastal areas. The total phenolic compound content found in each order is as follows: Suaeda japonica (3.674∼5.989 mg TAE/g dry wt.), Tetragonia tetragonoides (2.515∼4.471 mg TAE/g dry wt.), and Spergularia marina (2.450∼4.067 mg TAE/g dry wt.); DPPH radical scavenging and ABTS cation radical scavenging activities were also recorded in the same order. In the case of minerals, Iron(Fe) was relatively high in Suaeda japonica (960.1∼1,346.7 mg/kg), Manganese(Mn) in Tetragonia tetragonoides (197.1∼574.8 mg/kg), and Zinc(Zn) was relatively high in Spergularia marina (63.19∼216.56 mg/kg). It was confirmed that the mineral content of Suaeda japonica, Tetragonia tetragonoides, and Spergularia marina was significantly higher than that of Chinese cabbage, lettuce, leek, and spinach.

Targeting Multiple Myeloma Cell Death with Polyphenol EGCG

Tae‐Hoon Lee,Hee‐Young Yang,Hoon‐In Choi,Ung Yang,Kyoung‐Jin Chung,Lina Ren 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1

(‐)‐Epigallocatechin 3‐gallate (EGCG) is a potent antioxidant polyphenol in green tea that acts as an anticancer agent via both direct and indirect pathways. Although the relationship between EGCG’s anticancer effects and its antioxidant activity is not fully understood, it is known that EGCG stimulates production of reactive oxygen species (ROS), which induce oxidative stress leading to cell death. In IM9 multiple myeloma cells, EGCG acted in a dose‐ and time‐dependent manner to induce apoptotic cell death. Among the antioxidant enzymes expressed in IM9 cells, levels of peroxiredoxin (Prdx) V were selectively and significantly reduced by EGCG. Moreover, the ROS scavenger NAC completely inhibited EGCG‐induced apoptosis and PrdxV reduction, while overexpression of PrdxV, but not a PrdxVC48S mutant, protected IM9 cells from EGCG‐induced apoptosis. EGCG‐induced reductions in cell viability and PrdxV levels were also observed in primary CD138+ multiple myeloma cells from patients. These results suggest that PrdxV is a key target via which EGCG mediates its anticancer effects.

Peroxiredoxin V Regulates Aco2, Acadm, and Acox1 Activity in Hypoxic Kidney

Tae‐Hoon Lee,Hee‐Young Yang,Joseph Kwon,Hoon‐In Choi,Lina Ren,Ung Yang,Byung‐Ju Park,Zae Young Ryoo 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1

Peroxiredoxin V, an atypical thioredoxin peroxidase, is widely expressed in mammalian tissues. In addition, Prdx V is localized in mitochondria, peroxisome, cytosol, and nucleus. Prdx V has been reported to protect a wide range of cellular environments as antioxidant enzyme, and its dysfunctions may be implicated in several diseases, such as cancer, inflammation, and neurodegenerative disease. Identification and relative quantification of proteins affected by Prdx V may help identify novel signaling mechanisms that are important for oxidative stress response. However, the role of Prdx V in the modulation of hypoxia‐related cellular response is not studied yet. In order to examine the function of endogenous Prdx V in hypoxic condition in vivo, we generated a transgenic mouse model with Prdx V siRNA expression controlled by U6 promoter. Of many tissues, the knockdown of Prdx V expression was displayed in kidney, lung, and liver, but not spleen and skin. We conducted on the basis of nano‐UPLC‐MSE proteomic study to identify the Prdx V‐affected protein networks in hypoxic kidneys. In this study, we identified protein networks associated with oxidative stress, fatty acid metabolism, and mitochondrial dysfunction. Our results indicated that Prdx V affected to regulation of kidney homeostasis under hypoxia stress.

Proteomic Analysis of Thiol Specific‐Protein Oxidation

Tae‐Hoon Lee,Hee‐Young Yang,Hoon‐In Choi,Gia‐Buu Tran,Ung Yang 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1

Peroxiredoxin II (Prdx II; a typical 2‐Cys Prdx) has been originally isolated from erythrocytes, and its structure and peroxidase activity have been adequately studied. Mice absent to Prdx II proteins had heinz bodies in their peripheral blood, and morphologically abnormal cells were detected in the dense red blood cell (RBC) fractions, which contained markedly higher levels of reactive oxygen species (ROS). In this study, a labeling experiment with the thiol‐modifying reagent biotinylated iodoacetamide (BIAM) in Prdx I‒/‒ mice revealed that a variety of RBC proteins were highly oxidized. To identify oxidation‐sensitive proteins in Prdx II‒/‒ mice, we performed RBC comparative proteome analysis in membrane and cytosolic fractions by nano‐UPLC‐MSE shotgun proteomics. We found oxidation‐sensitive 54 proteins from 61 peptides containing cysteine oxidation, and analyzed comparative expression pattern in healthy RBCs of Prdx II+/+ mice, healthy RBCs of Prdx II‒/‒ mice, and abnormal RBCs of Prdx II‒/‒ mice. These proteins belonged to cellular functions related with RBC lifespan maintain, such as cytoskeleton, stress‐induced proteins, metabolic enzymes, signal transduction, and transporters. Furthermore, protein networks among identified oxidation sensitive proteins were analyzed to associate with various diseases. Consequently, we expected that RBC proteome may provide clues to understand redox‐imbalanced diseases.

Identification of Redox Sensitive Proteins in Prdx II Deficient Red Blood Cells

Tae‐Hoon Lee,Hee‐Young Yang,Hoon‐In Choi,Gia‐Buu Tran,Ung Yang 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1

Red blood cells (RBCs) have been studied as models for infectious diseases, various symptoms of anemia, hemolysis, and erythrocyte aging. Although do not directly affect RBCs, other diseases may cause RBC physiological alterations that could be advanced for diagnostic aim or to convince better understanding of a certain pathological pattern. In this study, comparative RBC proteomics between healthy and abnormal conditions involve to promote aging related‐biomarker discovery. Peroxiredoxin II (Prdx II; a typical 2‐Cys Prdx) has been originally isolated from erythrocytes, and its structure and peroxidase activity have been adequately studied. Mice absent to Prdx II proteins had heinz bodies in their peripheral blood, and morphologically aged cells were detected in the dense RBC fractions, which contained markedly higher levels of reactive oxygen species (ROS). In addition, a labeling experiment with the thiol‐modifying reagent biotinylated iodoacetamide (BIAM) in Prdx II‒/‒ mice revealed that a variety of RBC proteins were highly oxidized. To identify oxidation‐sensitive proteins in Prdx II‒/‒ mice, we performed RBC comparative proteome analysis by nano‐UPLC‐MSE shotgun proteomics with relative protein quantitative analysis. We found oxidation‐sensitive 18 membrane and 41 cytosol proteins from 32 and 85 peptides containing cysteine oxidation, and analyzed comparative expression pattern in healthy RBCs of Prdx II+/+ mice (W1), healthy RBCs of Prdx II‒/‒ mice (K1), and abnormal RBCs of Prdx II‒/‒ mice (K2). These proteins belonged to cellular functions related with RBC lifespan maintain, such as cytoskeleton, stress‐induced proteins, amino acid/nucleic acid metabolic enzymes, signal transduction, and molecular transporters. Furthermore, protein networks among identified oxidation sensitive proteins were analyzed to associate with aging consequence. Consequently, we expected that RBC proteome may provide clues to understand redox‐imbalanced diseases.

ZMP를 이용한 굴삭기의 안정성에 관한 연구

최종환,엄혁,임태형,김승수,양순용,이병룡,안경관 한국공작기계학회 2003 한국공작기계학회 춘계학술대회논문집 Vol.2003 No.-

The hydraulic excavator has been a popular research object for automation because of its multi-workings and economic efficiency. When it works crane tasks, most of disasters happen. The stability of the excavator having crane function has a close relation with excavator's posture, motion and load. In this paper, the stability of tipping-over has been analysed using Zero Moment Point(ZMP),

비틀림 방법을 통한 단일모드 광섬유의 미세 복굴절도의 측정

최태일,정해양 경희대학교 레이저공학연구소 2001 레이저공학 Vol.12 No.-

We describe a twist method for measuring ultralow birefingence(i.e. Δn□10-6~10-8) in single mode optical fibers. In twisted single-mode optical fibers the output state of polarization of light is affected by an elastooptically induced optical activity and by modification of any linear birefringence. The measuring procedure involves measurement of the light intensity only and does not require an analysis of the light-polarization state. Experimental results for commercial single mode fibers with Δn□2×10-6 are presented.

대인ㆍ대내인간 통합적 의사소통 프로그램 개발

최태산,양정남 동신대학교 2005 論文集 Vol.15 No.-

연구는 대인, 대내인간 통합적 의사소통 훈련 프로그램을 개발하기 위하여 실시되었다. 연구목적을 달성하기 위해 먼저 선행연구와 임상경험을 토대로 프로그램을 구성하였다. 구성된 프로그램의 효과는 동신대학교 남·여 대학생 30명을 실험집단과 통제집단으로 구분한 후, 총 24시간(6X4)의 프로그램을 실시하여 사전-사후간의 차이를 비교, 검증하였다. 측정도구는 문제해결에 대한 자기평가검사와 자아존중감검사를 사용하였는데, 결과를 요약하면 다음과 같다. 첫째, 대인간·대내인간 통합적 의사소통 훈련 프로그램이 지각된 문제해결능력에 미치는 효과를 검증한 결과, 유의수준 .001에서 통계적으로 유의미한 차이가 있었다. 둘째, 대내인간 통합적 의사소통 훈련 프로그램이 자아존중감에 미치는 효과를 검증한 결과, 유의수준 .001에서 통계적으로 유의미한 차이가 있었다. 따라서 대인간·대내인간 통합적 의사소통 훈련 프로그램은 문제해결능력과 자아존중감 향상에 효과가 있다고 할 수 있다. This study has a goal to develop of the Integrated(Inter·Intra) Communication Training Program. The Integrated(Inter·Intra) Communication Training Program based on preceding research and clinical experience was developed. The subject of this study were 30 Dongsin university students. They divided into experimental group and control group, and then experienced 24 hours program. to examine the effects of Measurement instruments were Problem Solving Inventory and Self-Esteem Inventory. The results are as follow: 1) The results of The Integrated(Inter·Intra) Communication Training Program at a problem solving ability is revealed on significant difference(p<.001). 2) The results of The Integrated(Inter·Intra) Communication Training Program at increasing of self-esteem is revealed on significant difference(p<.001). For that reason The Integrated(Inter·Intra) Communication Training Program is effective to problem solving ability and increasing of self-esteem.

産婦人科 患者에서 分離된 Ureaplasma urealyticum의 生物學的 性狀

梁熙燦,崔大卿,張明雄,白太鉉,朴鼎圭 충남대학교 의과대학 지역사회의학연구소 1986 충남의대잡지 Vol.13 No.2

Isolation rates of Ureaplasma urealyticum from vaginal swab of 216 patients, 105 prostitutes and 100 college females was investigated. Also, minimal inhibitory concentration of isolated strains about tetracycline and minocycline was studied. The results were summarized as follows; 1. Out of 70 vaginitis patients, isolation rates of Ureaplasma urealyticum and Mycoplasma hominis were 34.3% and 15.7%, respectively. 2. Out of 54 cervicitis patients, isolation rates of Ureplasma urealyticum and Mycoplasma hominis were 37.0% and 11.0%, respectively. 3. Out of 92 other gynecological patients, isolation rates of Ureaplasma urealyticum and mycoplasma hominis were 44.6% and 16.3%, respectively. 4. Out of 105 prostitues, isolation rates of Ureaplasma urealyticum and Mycoplasma hominis were 54.3% and 75.2%, respectively. 5. Out of 100 college students, isolation rates of Ureaplasma urealyticum and Mycoplasma hominis were 23.0% and 7.0% respectively. 6. In the susceptibility patterns of isolated Ureaplasma urealyticum, resistant strains about tetracycline in patients, prostitues and college students were 75.2%, 73.6% and 69.5%, respectively 7. In the susceptibility patterns of isolated Ureaplasma urealyticum, resistant strains about minocycline in patients, prostitues and college students were 91.7%, 87.7% and 73.9%, respectively.

한의진단명과 진단요건의 표준화 연구 III : 3차년도 연구결과 보고

최선미,양기상,최승훈,박경모,박종현,심범상,김성우,노석선,이인선,정진홍,이진용,김달래,임형호,김윤범,박성식,송태원,김종우,이승기,최윤정,신순식 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the third year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows : - analyzing and differentiating of epidemic febrile disease - analyzing and differentiating in accordance with the Sasang constitution medicine based on four-type recognition - differentiation of disease according to pathological changes of Chong and Ren channels - standards for diagnosis of women's disease - standards for diagnosis of children's disease - standards for diagnosis of motor and sensor disturbance (-muscle. born, joint, etc.) - standards for diagnosis of neuropsychiatric disease - standards for diagnosis of five sense organ disease - standards for diagnosis of external disease The indivisual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name, notion of diagnosis pattern, index of differentiation of symptoms and signs, the main point of diagnosis, analysis of diagnosis pattern, discrimination of diagnosis pattern, prognosis, a way of curing a disease, prescription, herbs in common use, disease appearing the diagnosis pattern, documents. The standards for diagnosis of each disease was arranged by the diagnostic requirements in the following order : another name, notion of disease, the main point of diagnosis, analyzing and differentiating of disease, analysis of disease, discrimination of disease, prognosis, a way of curing and prescription of disease, disease in western medicine appearing the disease in oriental medicine, documents.