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해수에서 분리한 Vibrio sp. M-96 균주의 열감수성 alkaline phosphatase 성질
박문경,진덕희,김중균,공인수,김광현,홍용기 동의대학교 기초과학연구소 1997 基礎科學硏究論文集 Vol.7 No.1
A thermolabile alkaline phosphatase has been purified through steps of osmotic shock, ammonium sulfate salting-out, and DEAE-cellulose chromatography from the cultured broth of the marine Vibrio sp. M-96 strain. The optimal temperature for the enzyme activity was 35℃. The optimal pH was pH 11.0, and the range of pH stability was pH 10.4 to 12.0. Thermal inactivation occured within 6 minutes at 60℃. The enzyme was considerably inactivated by 0.1mM concentrations of Hg^2+, Ni^2+ and Zn^2+, whereas activated up to 234% by 1mM of Mn^2+. The activation energy and deactivation energy by the Arrhenius equation were 4.02 Kcal/mol and 9.09 Kcal/mol, respectively. The Km and Vmax values of the enzyme for p-nitrophenylphosphate were found to be 0.0465mM and 0.001335mM/min. respectively. Active from of the enzyme had a molecular weight of 57,000 dalton determined by the Sephadex G-200 gel filtration method.
朴冕用,李文壽,鄭求春 建國大學校附設 應用科學硏究所 1978 理學論集 Vol.4 No.-
An infra-red absorption which is characteristics to the novolac resin is occurred at 1,000 ??, and this peak is useful for the identification of novolac phenol resin from resolic phenol resin. The relative intensities of infra-red absorption peaks at 813.01 and 751.88 ?? of novolac and resolic phenol resin samples did not show the significant changes for 60 min. at 140℃ and 120℃, but they were changed as a function of heat-treated time from 60 min. thereafter.
박문기,이현정,김광중,문영수 한국환경과학회 2005 한국환경과학회지 Vol.14 No.2
The concentration of heavy metal were studied for the estimation of quality in herbal medicines which is Bupleuri Radix, Paeoniae Radix, Dioscoreae Rhizoma and Astragali Radix in products of north Gyeongbuk area. Also, we collected soils from several area in Gyeongbuk, and determined heavy metal concentration of soils by ICP. We compared with herbal medicines in products of Gyeongbuk and their soils. The average levels of heavy metal of herbal medicines in Gyeongbuk area are as follows : Mercury is 0.037㎎/㎏, Chromium is 0.093 ㎎/㎏, Nickel is 0.108㎎/㎏, Copper is 0.475㎎/㎏, Zinc is 3.14㎎/㎏, Manganese is 1.52㎎/㎏, Iron is 7.83㎎/ ㎏, and Cadmium, Lead and Arsenic is not detected. It was very lower in concentrations than those of average Korea area. For the heavy metals contents of Gyeongbuk area, in Gong Jung Ri, represented the higher level, it may be due to a near the road. The Cd contents in soil was not a wide difference. For the As and Pb contents in soil, Song Sa Ri (Bupleuri Radix) represented the higher level(0.68㎎/㎏, 1523㎎/㎏). Correlation coefficients of heavy metal contents in cultured herbal medicines and their soils did not give reproducible results. But the degree of correlation between a few heavy metal contents of Dioscoreae Rhizoma and their soil showed the good transfer from the soil.
박용,조문수 大邱大學校 科學技術硏究所 1999 科學技術硏究 Vol.5 No.5
The cultural method of shoot primordia was developed to maintain parental lines of F₁ hybrids for a long time without genetic variation. The shoot primordium including 1 or 2 leaf primordia was cultured in the semi-solid MS basal media with various combinations of BAP and kinetin. The cultures were maintained and proliferated in the 2 rpm drum shaker with illumination of 1,000 and 2,000 lux on the upper and lower part, respectively. Cultivar, Shinkuroda and male sterile lines had higher percentages of cell clumps than cultivars, Kazan and Flakkee-Vita-Longa. Higher concentrations of BAP and NAA showed higher percentages of cell clumps. C8(2.0mg/L BAP + 0.2mg/L NAA) was very effective in the percentages of cell clumps in all lines. Shinkuroda had higher percentages of plant regeneration among tested materials. Higher concentration of BAP and kinetin had higher percentages of plant regeneration. AR line has 93% of cell clump formation and 43% of plant regeneration. Plantlets has a average of 6 shoots and 3 roots.
박영성,서문삼,강기혁,김봉수 大田大學校 環境問題硏究所 2002 환경문제연구소 논문집 Vol.6 No.-
γ-Al_2O_3에 몰리브덴을 담지시킨 촉매를 고정층 반응기에 충전하여 벤젠과 같은 VOCs의 촉매산화반응 특성을 살펴보았다. 실험조건은 반응온도를 200∼500℃로 하고, 반응물인 벤젠의 농도를 1,000∼3,000ppm, 공간속도를 5,000∼60,000hr^-1의 범위에서 적용하였다. 연구결과, 벤젠의 전환율은 공간속도가 낮을수록 증가하고, 초기유입농도가 증가할수록 감소하는 것으로 나타났다. 몰리브덴이 담지된 촉매의 성능은 니켈을 제외한 구리나 망간등의 다른 전이금속에 대해서는 다소 떨어지는 것으로 나타났다. 벤젠의 촉매연소반응은 1차 균일반응으로 해석될 수 있었으며, 균일연소반응의 활성화 에너지값은 7.4kcal/㏖ 이었다. Catalytic oxidation characteristics of benzene (one of important source of volatile organic compounds : VOCs) was investigated in a fixed bed flow reactor using Mo/γ-A1_2O_3 catalyst. As operating variables of experiment, reaction temperature (200∼500℃), benzene concentration (1,000∼3,000ppm), space velocity (5,000∼60,000hr^-1) were applied. The experimental results showed that the conversion of catalytic oxidation of benzene was increased with decreasing space velocity, while it was decreased with increasing inlet VOCs concentration. Mo/γ-A1_2O_3 catalyst exhibited more or less low activity among several transition metals such as Cu, Mn, Cr, etc. except Ni. The catalytic oxidation reaction for VOCs(benzene) could be expressed as the first order homogeneous reaction the activation energy of which was 7.4kcal/㏖.
문수덕,박재일,최경,황희용 강원대학교 정보통신연구소 2005 정보통신논문지 Vol.9 No.-
In this paper, A ultra wideband antenna with electromagnetically coupled feeding is presented. The designed antenna is composed of Bow-tie patch with parasitic patch ground of a semicircular shape. The measured bandwidth of the designed antenna is from 2.5GHz to 8.3GHz for -10dB return loss (VSWR < 2). This antenna shows a similar radiation pattern of monopole and maximum gain better than ideal dipole throughout the operating frequency band.
문수연,최영실,박미연,이정아,정미경,정혜숙,정두련,송재훈,백경란 대한감염학회 2009 감염과 화학요법 Vol.41 No.3
Q fever is a zoonosis caused by Coxiella burnetii, Presenting as acute and chronic illness and it has been reported worldwide. Acute Q fever is usually asymptomatic or mild and self-limiting, but infective endocarditis is one of the most serious complications of chronic Q fever and can be fatal. Known risk factors for Q fever endocarditis are valvular heart disease, immunocompromised hosts, and pregnancy. There have been some reports on Q fever in Korea but there exists no report on Q fever endocarditis. We have experienced 2 cases of Q fever with underlying valvular heart disease; both Patients came to the hospital for evaluation of prolonged fever. Although Q fever and Q fever endocarditis are rare in Korea, Q fever endocarditis should be considered in the differential diagnosis of patient with infective endocarditis when causative microorganism cannot be identified.