DJ-1/park7 modulates vasorelaxation and blood pressure via epigenetic modification of endothelial nitric oxide synthase

Won, Kyung Jong,Jung, Seung Hyo,Jung, Soo Hyun,Lee, Kang Pa,Lee, Hwan Myung,Lee, Dong-Youb,Park, Eun-Seok,Kim, Junghwan,Kim, Bokyung Oxford University Press 2014 Cardiovascular research Vol.101 No.3

<P><B>Aims</B></P><P>DJ-1/park7, a multifunctional protein, may play essential roles in the vascular system. However, the function of DJ-1/park7 in vascular contractility has remained unclear. The present study was designed to investigate whether the DJ-1/park7 is involved in the regulation of vascular contractility and systolic blood pressure (SBP).</P><P><B>Methods and results</B></P><P>Norepinephrine (NE) elevated contraction in endothelium-intact vessels in a dose-dependent manner, to a greater extent in DJ-1/park7 knockout (DJ-1/park7<SUP>−/−</SUP>) mice than in wild-type (DJ-1/park7<SUP>+/+</SUP>) mice. Acetylcholine inhibited NE-evoked contraction in endothelium-intact vessels, and this was markedly impaired in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP>. Nitric oxide (NO) production (82.1 ± 2.8% of control) and endothelial NO synthase (eNOS) expression (61.7 ± 8.9%) were lower, but H<SUB>2</SUB>O<SUB>2</SUB> production (126.4 ± 8.6%) was higher, in endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice than in those from DJ-1/park7<SUP>+/+</SUP> controls; these effects were reversed by DJ-1/park7-overexpressing endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice. Histone deacetylase (HDAC)-1 recruitment and H3 histone acetylation at the eNOS promoter were elevated and diminished, respectively, in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls. Moreover, SBP was significantly elevated in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls, but this elevation was inhibited in mice treated with valproic acid, an inhibitor of Class I HDACs including HDAC-1.</P><P><B>Conclusion</B></P><P>These results demonstrate that DJ-1/park7 protein may be implicated in the regulation of vascular contractility and blood pressure, probably by the impairment of NO production through H<SUB>2</SUB>O<SUB>2</SUB>-mediated epigenetic inhibition of eNOS expression.</P>

비매너 주차 단속시스템

박상민(Sang-min Park),손병수(Byung-Soo Son),김명식(Myung-sik Kim),최병윤 한국정보통신학회 2021 한국정보통신학회 종합학술대회 논문집 Vol.25 No.1

주차장에서 일어날 수 있는 비매너 주차로 인한 충돌을 사전에 예방하기 위한 단속시스템이다. 주차장에서 일어날 수 있는 장애인, 전기차 전용 주차구역에 일반차량 주차, 2개 차선을 물고 주차하는 차량이 있다. 위와 같은 차량을 딥러닝 객체인식 기능을 통해 비매너 주차를 감지하여 알려준다. 비매너 주차 상황이 찍힌 사진이나 영상을 학습데이터로 사용하여 상황을 인식할 수 있도록 학습데이터를 제작하고 그 상황을 인식하여 비매너 주차 유무를 판단한다. 주차장의 환경을 좀 더 쾌적하게 함으로써 주차장 이용자간 충돌을 줄이는데 목적이 있다. It is a enforcement system to prevent collisions caused by unmanageable parking that may occur in parking lots. There are handicapped people who can get up in parking lots, general vehicles parked in electric vehicle parking areas, and vehicles parked in two lanes. The vehicle above is detected and notified through the deep learning object recognition function. By using a picture or video of an unmanageable parking situation as learning data, the learning data is produced so that the situation can be recognized, and the situation is recognized to determine the presence or absence of unmanageable parking. The purpose is to reduce collisions between parking lot users by making the environment of the parking lot more comfortable.

이명직의 성서강해 연구

박명수(Park Myung-Soo) 한국기독교역사연구소 2004 한국기독교와 역사 Vol.21 No.-

The purpose of this paper is to describe and analyse several Bible expositions (Matthew, Daniels, Songs of Solomon, Romans, Ruth, Hosea, Revelation) written by Lee, Myung-Chick during 1920s. Rev. Lee has been called the father of the Holiness Church for long time. He had been the chief administrator of the Holiness Church and the principal of the Kyung-Sung Bible School (now, Seoul Theological University) for several decades. However, he was also the famous Bible expositor in Korean Church. He wrote many Bible expositions in his life and published them in monthly the Living Water. His exposition of the Bible had influenced the Holiness Church as well as the Korean church in general. In 1920s, Korean society confronted secularization of western culture, communism from Russia, and liberal theology of western church. In this context, Lee had tried to maintain the Full (or pure) Gospel of the Bible. He had criticized secularization very strongly, considered communism as anti-Christ, and attacked liberal theology as a threat of Christianity. After description and analysis of the Bible exposition, the writer concludes the main characteristics of the Bible expositions by Lee, Myung-Chick as followings. First, Rev. Lee tried to read the Old Testament from Christological perspectives. He found many Christological figure in the Old Testament. Second, he stood in the tradition of the Wesleyan Holiness movement, key teaching of which is the Holiness experience by the Baptism of the Holy Spirit. Third, his framework of the Bible interpretation is premillennialism. For Rev. Lee, Chiristian should live in the shadow of the second coming of Christ. Fourth, his emphasis on holiness and the second coming of Christ led to attack secularization as anti-Christ. Christian should not follow this world. Here Holiness movement considered as anti-secularization movement. Finally, Rev. Lee explained the Bible from woman’s perspectives. Of course, Rev. Lee is not feminist. However, all Christian should be a bride of the coming Christ, the bridegroom of all believers. For him, Daniels, Ruth, and the woman of Solomon in the Songs were the examples of true bride of Christ. Church history sometimes considers as history of the Bible interpretation. The Bible interpretation principles of Rev. Lee had heavily influenced the Korean Holiness Church.

捻轉法이 竝行된 鍼刺戟 및 電鍼刺戟이 鎭痛效果에 미치는 影響

Park, Dong-Seok,Kim, Ji-Hoon,Min, Byung-Il,Oh, Soo-Myung 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Dong-Seok Park¹, Ji-Hoon Kim¹ and Byung-Il Min, Soo-Myung Oh,³¹Department of Acupuncture and Moxibustion, College of Medicine, ²Department of Physiology, College of Medicine, And ³East-West Medical Research Institute, Kyung Hee University,Seoul, Korea. Analgesic Effect of Manual Acupuncture and Electroacupuncture combined with Twirling Method. Proceeding of International Symposium on East-West Medicine Seoul. 187-200, 1999.-Twirling the needle (TN) is one of several needing methods frequently used for acupuncture in the oriental medicine. The present study was conducted to see if TN enhanced the antinociceptive effect produced by plain-or electroacupuncture in the rat tail flick test. Rats (Sprague-Dawley, 200-300g)lightly anesthetized with thiopental sodium(40mg/kg, i.p.) were used in the tail-flick test. The basal reaction time for tail-flick was 2 sec. For "plain" acupuncture(PA), a needle was inserted for 20 min into a Chock-samni (ST36) acupuncture point which (is located at the anterior tibial muscle and about 10mm below the knee joint). For electroacupuncture(EA), train-pulses(3 Hz,0.3-ms pulse width, 0.2-0.3mA) were applied to the inserted needle for the period of insertion. For combining TN with either PA or EA, TN was performed during the each acupuncture period. Two methods for TN were used; twirling 3 times for 1 second persisted for 1 min in every 5 mins(long-duration and long-interval;LDLI) and twirling 3 times for 1 second persisted for 10 sec in every 1 min(short-duration and short-interval;SDSI). PA and EA produced an increase in tail flick latency (TFL); peak increases were 21.3±5.1% and 42.1±17.3% of the pre-acupunture control, respectively, and occurred immediately after cessation of 20min-acupuncture. Performing TN combined with PA increased TFL more than PA itself by showing a greater peak increase in TFL when performed with a SDSI-TN than with a LDLI-TN(60.6±12.5% and 42.7±22.7% of the pre-acupuncture control, respectively). Performing TN combined with EA also increased TFL more than EA itself and a greater peak increase in TFL was observed with a SDSI-TN as compared to a LDLI-TN(77.5±13.8% and 67.3±14.0% of the pre-acupuncture control, respectively). These results indicate that TN enhances both PA-and EA-produced antinociception where an greater enhanced antinociception than a LDLI-TN. It is suggested that performing a SDSI-TN combined with EA seems to be one of the most effective ways to produce analgesia.

이명직 목사와 일제시대 한국성결교회의 부흥운동

박명수 ( Myung Soo Park ) 현대기독교역사연구소 2002 성결교회와 신학 Vol.7 No.-

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

IMF 전·후의 정부행정개혁에 관한 비교연구 : 김영삼정부와 김대중정부의 개혁을 중심으로

박명수 중앙대학교 산업경영연구소 1999 산업경영연구 Vol.8 No.2

IMF 통제 및 관리체제라는 특정한 시대적 배경에서 본 연구는 김영삼 정부의 행정개혁과 김대중 정부의 1, 2차 정부조직개편에 따른 행정개혁을 비교 ·분석함으로써 나타나는 개혁의 특징과 문제점을 기술하고, 이에 따라 과거 행정개혁의 특징을 행태론적 측면에서 논의하는데 그 목적이 있다. 우리 나라의 행정개혁은 정치권과 이익집단의 개입으로 처음 계획된 것과는 다르게 많은 난관에 부딪히면서 변화해왔지만 계속적인 정부개혁과 유연성있는 대처를 필요로 하며 조직개편만이 관료들의 무기력한 행태를 개혁할 수 있다는 생각으로부터 자아실현적 방법과 변화의 동조자라는 관료의 인식과 확신을 가지는 일 또한 수반되어야 할 것이다. 따라서, IMF 前後 양(兩) 정부의 조직개편에서 나타난 바와 같이 부서를 줄이고 직급을 조정하는 행정개혁만으로는 바람직한 관려들의 행태가 이루어지지 않다는 점을 다시 한번 명심해야 할 것이다.

지구성 운동이 중년여성의 혈중지질농도에 미치는 영향

朴麟基,崔大雨,李明秀,徐在河,趙炳俊,金泰亨 충남대학교 체육과학연구소 2000 體育科學硏究誌 Vol.18 No.1

The purpose of this study was to identify the effect of endurance exercise program on the middle-aged women. Six subjects were recruited from among volunteers responding to advertisements placed around the university community. All subjects, with no experience in regular physical activity participated in an interventional training consisting of swimming and stretching three days a week for 10weeks. Pre-and post blood lipid levels, such as Total Cholesterol(TC), High Density Lipoprotein Cholesterol(HDL-C), Low Density Lipoprotein Cholesterol(LDL-C), and Triglyceride(TG) were measured. A paired t-test showed results that were significant the .05 level were HDL-C , LDL-C, TG, and TC/HDL-C Ratio. The main finding of the study was that the role of endurance training, as prescribed in our study has the effect of making improvements in blood lipid levels for the middle-aged women. 1. After a 10week period of endurance exercise, the Total Cholesterol(TC) level was higher than that of pre-test. But the difference no showed the statistical significance. 2. After a 10week period of endurance exercise, the High Density Lipoprotein Cholesterol(HDL-C) Low Density Lipoprotein Cholesterol(LDL-C). Triglyceride(TG) level was higher than that of pre-test. and the difference showed the Statistical significance. In conclusion, even though there was significant in blood lipid levels after endurance exercise, swimming has a beneficial on improving in the middle-aged women.

刑事司法行政 運營과 人權問題

朴命洙 中央大學校 法學硏究所 1990 法學論文集 Vol.15 No.-

金屬薄膜導波管을 利用한 I/O Mode Filter

박한규,오한솔,김명수 연세대학교 산업기술연구소 1981 논문집 Vol.13 No.2

代表的인 3종류의 金屬薄膜導波管의 TEo-Mode와 TMo-Mode의 특성을 Computer Simulation에 의하여 비교 고찰하였다. 非對稱型平板薄膜導波管으로된 금속박막도파관은 TEo-Mode Filtering이 不可能하였다. 對稱型平板薄膜導波管으로된 금속박막도파관에서는 Film층과 그 외의 층과 굴절율 차이가 0.02 이하가 되면 TEo-Mode Filtering이 가능하며 TMo-Mode 의 損失이 10dB/cm이하가 되어 Mode Filter로서 使用可能함이 밝혀졌다. The typical three kinds of the Metal-Clad waveguides have been chosen, and the characteristics of TEo-Mode and TMo-Mode in them are investigated and compared with each other by computer simulation. TEo-Mode filtering has been impossible in the Metal-Clad waveguide made of the symmetric thin film slab waveguide. In the Metal-Clad waveguide made of the symmetric thin film slab waveguide, if the refraction index difference between the film layer and the other layers is below 0.02, it has made TEo-Mode filtering possible and has had TMo-Mode loss below 10dB/㎝. Therefore it has been improved that it can be used practically as a Mode Filter.