Measurement of|Vub|from Inclusive Charmless SemileptonicBDecays

Urquijo, P.,Barberio, E.,Adachi, I.,Aihara, H.,Arinstein, K.,Bakich, A. M.,Belous, K.,Bhardwaj, V.,Bischofberger, M.,Bozek, A.,Brač,ko, M.,Browder, T. E.,Chao, Y.,Chen, A.,Cheon, B. G.,Chistov, R American Physical Society 2010 Physical review letters Vol.104 No.2

<P>We present the partial branching fraction for inclusive charmless semileptonic B decays and the corresponding value of the Cabibbo-Kobayashi-Maskawa matrix element vertical bar V-ub vertical bar, using a multivariate analysis method to access similar to 90% of the B -> X(u)l nu phase space. This approach dramatically reduces the theoretical uncertainties from the b-quark mass and nonperturbative QCD compared to all previous inclusive measurements. The results are based on a sample of 657 X 10(6) B (B) over bar pairs collected with the Belle detector. We find that Delta B(B -> X(u)l nu; p(l)*(B) > 1.0 GeV/c) = 1.963X(1 +/- 0.088(stat) +/- 0.081(syst)) X 10(-3). Corresponding values of vertical bar V-ub vertical bar are extracted using several theoretical calculations.</P>

New Evidence of Alleles (V199I and G52S) at the PRKAG3 (RN) Locus Affecting Pork Meat Quality

Chen, J.F.,Dai, L.H.,Peng, J.,Li, J.L.,Zheng, R.,Zuo, B.,Li, F.E.,Liu, M.,Yue, K.,Lei, M.G.,Xiong, Y.Z.,Deng, C.Y.,Jiang, S.W. Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.4

The porcine PRKAG3 (RN) gene encodes the regulatory gamma subunit of adenosine monophosphate-activated protein kinase (AMPK), which is a good candidate gene affecting meat quality. In this study, the effects of two missense mutations A595G (Ile199Val) and G154A (Gly52Ser) in porcine PRKAG3 gene on meat quality traits were studied in M. Longissimus dorsi (LD), M. Semispinalis capitis (SC) and M. Biceps femoris (BF) from different populations of 326 pigs. The PRKAG3 alleles 199I, 199IV, 52S and 52G were identified with PCR-RFLPs and all genotypes - 199I/199I, 199I/199V, 199V/199V, 52S/52S, 52S/52G and 52G/52G - were found. The frequency of V allele was larger than that of I allele in all populations. I allele frequency was zero in Chinese Meishan pigs (population D) especially. G allele frequency was larger than that of S allele in all populations except Large White (population A). Both variations at the PRKAG3 locus significantly affected these meat quality traits. The pork meat quality has not previously been established in Meishan or crosses thereof. The results suggested that generally pH of LD, SC and BF was higher in Meishan pigs than that in other populations. Moreover, Meishan pigs showed higher water-holding capacity and intramuscular fat (IMF), lower water content and water loss percentage compared to other populations in terms of the two variations. The results present here supply new evidence that alleles V199I and G52S at the PRKAG3 locus affect pork meat quality and provide useful information on pork production.

Large-scale dataset from China gives new insights into leaf margin-temperature relationships

Chen, Wen-Yun,Su, Tao,Adams, Jonathan M.,Jacques, Fré,dé,ric M.B.,Ferguson, David K.,Zhou, Zhe-Kun Elsevier 2014 Palaeogeography, palaeoclimatology, palaeoecology Vol.402 No.-

Leaf margin analysis (LMA) is an important method of estimating paleotemperatures from fossil leaf floras or modern floras. Although some calibration has been carried out based upon large-scale studies of modern forests, most of this research has been in North America and Europe, with relatively little calibration work in East Asia. In the present study, we used species range maps of 3116 native dicot trees of China to derive synthetic local floras for each county from the Chinese humid region, and compared the percentage of untoothed leaf margined species with several temperature and precipitation related parameters. The results confirm the generally strong relationship between the proportion of species with untoothed leaf margins and climatic parameters within China. Leaf habit (deciduous vs. evergreen) does not strongly affect this relationship. The transfer function obtained from China, while not identical, is similar to those obtained from other regions, and is affected by regional restrictions, such as complex topography and relic taxa. As such it clarifies the potential range of error inherent in the LMA method as applied to paleodimate reconstruction. It is possible, however, that with the close similarity of the modern Chinese tree floras to Neogene floras in the Northern Hemisphere, the present estimate offers a better transfer function for reconstructing the Neogene paleoclimate in various regions without extremely cold conditions across the Northern Hemisphere. (C) 2014 Published by Elsevier B.V.

Transcriptomes of major renal collecting duct cell types in mouse identified by single-cell RNA-seq

Chen, Lihe,Lee, Jae Wook,Chou, Chung-Lin,Nair, Anil V.,Battistone, Maria A.,Pă,unescu, Teodor G.,Merkulova, Maria,Breton, Sylvie,Verlander, Jill W.,Wall, Susan M.,Brown, Dennis,Burg, Maurice B. National Academy of Sciences 2017 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.114 No.46

<P><B>Significance</B></P><P>A long-term goal in mammalian biology is to identify the genes expressed in every cell type of the body. In the kidney, the expressed genes (i.e., transcriptome) of all epithelial cell types have already been identified with the exception of the cells that make up the renal collecting duct, which is responsible for regulation of blood pressure and body fluid composition. Here, single-cell RNA-sequencing was used in mouse to identify transcriptomes for the major collecting duct cell types: type A intercalated cells, type B intercalated cells, and principal cells. The information was used to create a publicly accessible online resource. The data allowed identification of genes that are selectively expressed in each cell type, which is informative for cell-level understanding of physiology and pathophysiology.</P><P>Prior RNA sequencing (RNA-seq) studies have identified complete transcriptomes for most renal epithelial cell types. The exceptions are the cell types that make up the renal collecting duct, namely intercalated cells (ICs) and principal cells (PCs), which account for only a small fraction of the kidney mass, but play critical physiological roles in the regulation of blood pressure, extracellular fluid volume, and extracellular fluid composition. To enrich these cell types, we used FACS that employed well-established lectin cell surface markers for PCs and type B ICs, as well as a newly identified cell surface marker for type A ICs, c-Kit. Single-cell RNA-seq using the IC- and PC-enriched populations as input enabled identification of complete transcriptomes of A-ICs, B-ICs, and PCs. The data were used to create a freely accessible online gene-expression database for collecting duct cells. This database allowed identification of genes that are selectively expressed in each cell type, including cell-surface receptors, transcription factors, transporters, and secreted proteins. The analysis also identified a small fraction of hybrid cells expressing aquaporin-2 and anion exchanger 1 or pendrin transcripts. In many cases, mRNAs for receptors and their ligands were identified in different cells (e.g., <I>Notch2</I> chiefly in PCs vs. <I>Jag1</I> chiefly in ICs), suggesting signaling cross-talk among the three cell types. The identified patterns of gene expression among the three types of collecting duct cells provide a foundation for understanding physiological regulation and pathophysiology in the renal collecting duct.</P>

Improvement of lower hybrid current drive systems for high-power and long-pulse operation on EAST

Wang M.,Liu L.,Zhao L.M.,Li M.H.,Ma W.D.,Hu H.C.,Wu Z.G.,Feng J.Q.,Yang Y.,Zhu L.,Chen M.,Zhou T.A.,Jia H.,Zhang J.,Cao L.,Zhang L.,Liang R.R.,Ding B.J.,Zhang X.J.,Shan J.F.,Liu F.K.,Ekedahl A.,Gonich 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.11

Aiming at high-power and long-pulse operation up to 1000 s, some improvements have been made for both 2.45 GHz and 4.6 GHz lower hybrid (LH) systems during the recent 5 years. At first, the guard limiters of the LH antennas with graphite tiles were upgraded to tungsten, the most promising material for plasma facing components in nuclear fusion devices. These new guard limiters can operate at a peak power density of 12.9 MW/m2 . Strong hot spots were usually observed on the old graphite limiters when 4.6 GHz system operated with power >2.0 MW [B. N. Wan et al., Nucl. Fusion 57 (2017) 102019], leading to a reduction of the maximum power capability. With the new limiters, 4.6 GHz LH system, the main current drive (CD) and electron heating tool for EAST, can be operated with power >2.5 MW routinely. Long-pulse operation up to 100 s with 4.6 GHz LH power of 2.4 MW was achieved in 2021 and the maximal temperature on the guard limiters measured by an infrared (IR) camera was about 540 C, much below the permissible value of tungsten material (~1200 C). A discharge with a duration of 1056 s was achieved and the 4.6 GHz LH energy injected into the plasma was up to 1.05 GJ. Secondly, the fully-activemultijunction (FAM) launcher of 2.45 GHz system was upgraded to a passive-active-multijunction (PAM), for which the density of optimum coupling was relatively low (below the cut-off value). Good coupling with reflection coefficient ~3% has been achieved with plasma-antenna distance up to 11 cm for the new PAM. Finally, in order to eliminate the effect of ion cyclotron range of frequencies (ICRF) wave on 4.6 GHz LH wave coupling, the location of the ICRF launcher was changed to a port that is located 157.5 toroidally from the 4.6 GHz LH system and is not magnetically connected

Search forB0→pΛ¯π−γat Belle

Lai, Y.-T.,Wang, M.-Z.,Adachi, I.,Aihara, H.,Asner, D. M.,Aulchenko, V.,Aushev, T.,Bakich, A. M.,Bala, A.,Bhuyan, B.,Bobrov, A.,Bozek, A.,Brač,ko, M.,Browder, T. E.,Chang, P.,Chekelian, V.,Chen, American Physical Society 2014 PHYSICAL REVIEW D - Vol.89 No.5

<P>We search for the charmless B-o decay with final state particles p (Lambda) over bar pi(-)gamma using the full data sample that contains 772 x 10(6)B (B) over bar pairs collected at the Upsilon(4S) resonance with the Belle detector at the KEKB asymmetric-energy e(+)e(-) collider. This decay is predicted to proceed predominantly via the b -> s gamma radiative penguin process with a high energy photon. No significant signal is found. We set an upper limit of 6.5 x 10(-7) for the branching fraction of B-o -> p (Lambda) over bar pi(-)gamma at the 90% confidence level.</P>

Investigation of PCR-RFLPs within Major Histocompatibility Complex B-G Genes Using Two Restriction Enzymes in Eight Breeds of Chinese Indigenous Chickens

R. F. Xu,K. Li,G. H. Chen,B. Y. Z. Qiang,D. L. Mo,B. Fan,C. C. Li,M. Yu,M. J. Zhu,T. A. Xiong,B. Liu 아세아·태평양축산학회 2005 Animal Bioscience Vol.18 No.7

New polymorphism of major histocompatibility complex B-G genes was investigated by amplification and digestion of a 401bp fragment including intron 1 and exon 2 using polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP) technique with two restriction enzymes of Msp I and Tas I in eight breeds of Chinese indigenous chickens and one exotic breed. In the fragment region of the gene, three novel single nucleotide polymorphisms (SNPs) were detected at the two restriction sites. We found the transition of two nucleotides of A294G and T295C occurred at Tas I restriction site, and consequently led to a nonsynonymous substitution of asparagine into serine at position 54 within the deduced amino acid sequence of immunoglobulin variableregion- like domain encoded by the exon 2 of B-G gene. It was observed at rare frequency that a single mutation of A294G occurring at the site, also caused an identical substitution of amino acid, asparagine 54-to-serine, to that we described previously. And the transversion of G319C at Msp I site led to a non-synonymous substitution, glutamine 62-to-histidine. The new alleles and allele frequencies identified by the PCR-RFLP method with the two enzymes were characterized, of which the allele A and B frequencies at Msp I and Tas I loci were given disequilibrium distribution either in the eight Chinese local breeds or in the exotic breed. By comparison, allele A at Msp I locus tended to be dominant, while, the allele B at Tas I locus tended to be dominant in all of the breeds analyzed. In Tibetan chickens, the preliminary association analysis revealed that no significant difference was observed between the different genotypes identified at the Msp I and Tas I loci and the laying performance traits, respectively.

Au-MPY/DTNBΣiO₂ SERS nanoprobe for immunosorbent assay

Chen, L.,Sa, Y.,Park, Y.,Hwang, H.,Ji, H.,Zhao, B.,Jung, Y.M. Elsevier 2016 Vibrational spectroscopy Vol.87 No.-

<P>In this paper, we developed a biocompatible surface-enhanced Raman scattering (SERS) nanoprobe that employs 4-mercaptopyridine (MPY)/5,5-dithiobis(2-nitrobenzoic acid) (DTNB)-decorated gold aggregates embedded in silicon dioxide. This highly sensitive SERS nanoprobe was applied in immunoassays. Furthermore, we constructed a sandwich (silver-proteins-Au-MPY/DTNB@SiO2) model that contributes very strong electromagnetic (EM) fields. Compared with a single layer of the SERS-active substrate, the designed model exhibited highly sensitive detection capabilities. This method demonstrates considerable potential for ultrasensitive SERS immunoassay applications. In addition, the proposed silica protected gold nanoaggregates possess good biocompatibility with proteins. (C) 2016 Elsevier B.V. All rights reserved.</P>

간호대생과 비보건계열 여대생의 미세먼지 위험에 대한 인식과 지식, 건강 관리행위 비교 연구

김선미,김슬기,김진선,박주형,배다희,유수빈,유영주,임지향,최유진,Chen Mengyao,양숙자,손지희 이화여자대학교 간호과학대학 2019 이화간호학회지 Vol.- No.53

Purpose: This study aimed to know whether there is a comparison of perception, knowledge and health promoting behaviors about particular matter between nursing students and non-health departments college students. Methods: A quantitative research is used. Data were collected from August 1 to 19. The study sample was nursing students and non-health departments students. For the similar condition of samples, this study excluded whose first language is not Korean. Independent T-test and pearson’s correlation was used for this study. Results: A significant positive correlation did not existed between difference of major and perception(t=0.017, p=0.986), knwledge(t=0.652, p=.516) and health promoting behaviors(t=-0.114, p=.910) about particular matters. Conclusion: Based on the study results, there is no significant correlation of perception and actions about particular matters between nursing students and non-health department college students.

Portable, non-destructive tester integrating VIS/NIR reflectance spectroscopy for the detection of sugar content in Asian pears

Choi, J.H.,Chen, P.A.,Lee, B.,Yim, S.H.,Kim, M.S.,Bae, Y.S.,Lim, D.C.,Seo, H.J. International Society for Horticultural Science ; 2017 Scientia horticulturae Vol.220 No.-

<P>In this study, a portable, non-destructive tester, integrating near-infrared (NIR) spectroscopy, was developed to determine the maturity of Asian pears. In addition, regression analysis was conducted for mature Manpungbae pears using the partial least squares regression model with the Brix value of Asian pears in a wavelength range of 710-930 nm, and the standard error of calibration or standard error of prediction (SEC/SEP) of 0.41-0.43 degrees Brix and relative standard error of calibration or relative standard error of prediction (rSEC/rSEP) = 0.91-0.92 were observed, indicating excellent prediction performance with no dependence on pretreatment conditions. Furthermore, the reliability of the developed Brix determination model was confirmed using Manpungbae, Wonhwang, Chuhwangbae, and Niitaka pears, and SEP, rSEP, and bias values of 0.29-0.33 degrees Brix, 0.90-0.96, and 1.67E-04, respectively, were observed, indicating high reliability. Hence, the construction of a Brix determination model by the accumulation of absorbance for several varieties of pears in each harvest period is expected to determine optimum harvest times via the measurement of the Brix values at full maturity regardless, of the variety. (C) 2017 Elsevier B.V. All rights reserved.</P>