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Grégoire Burgé,Claire Saulou-Bérion,Marwen Moussa,Florent Allais,Violaine Athes,Henry-Eric Spinnler 한국미생물학회 2015 The journal of microbiology Vol.53 No.10
The aims of this study is to compare the growth and glucose metabolism of three Lactobacillus reuteri strains (i.e. DSM 20016, DSM 17938, and ATCC 53608) which are lactic acid bacteria of interest used for diverse applications such as probiotics implying the production of biomass, or for the production of valuable chemicals (3-hydroxypropionaldehyde, 3-hydroxypropionic acid, 1,3-propanediol). However, the physiological diversity inside the species, even for basic metabolisms, like its capacity of acidification or glucose metabolism, has not been studied yet. In the present work, the growth and metabolism of three strains representative of the species diversity have been studied in batch mode. The strains were compared through characterization of growth kinetics and evaluation of acidification kinetics, substrate consumption and product formation. The results showed significant differences between the three strains which may be explained, at least in part, by variations in the distribution of carbon source between two glycolytic pathways during the bacterial growth: the phosphoketolase or heterolactic pathway (PKP) and the Embden-Meyerhof pathway (EMP). It was also shown that, in the context of obtaining a large amount of biomass, DSM 20016 and DSM 17938 strains were the most effective in terms of growth kinetics. The DSM 17938 strain, which shows the more significant metabolic shift from EMP to PKP when the pH decreases, is more effective for lactate production.
Lara Burg,Maite Timmermans,Maaike van der Aa,Dorry Boll,Koen Rovers,Ignace de Hingh,Anne van Altena 대한부인종양학회 2020 Journal of Gynecologic Oncology Vol.31 No.5
Objective: Peritoneal metastases (PM) are a challenge in gynecological cancers, but itsappearance has never been described in a population-based study. Therefore, we describe theincidence of PM and identify predictors that increase the probability of peritoneal spread. Methods: All ovarian, endometrial and cervical cancer patients diagnosed in the Netherlandsbetween 1989 and 2015 were identified from the Netherlands Cancer Registry and stratifiedfor PM. Crude and age-adjusted incidence over time was calculated. Independent predictorsfor PM were identified using uni- and multivariable analyses. Results: The 94,981 patients were diagnosed with ovarian, endometrial or cervicalcancer and respectively 61%, 2% and 1% presented with PM. Predictors for PM in ovariancancer were: age between 50 and 74 years (odds ratio [OR]=1.19; 95% confidence interval[CI]=1.08–1.32), other distant metastases (OR=1.25; 95% CI=1.10–1.41), poor differentiationgrade (OR=2.00; 95% CI=1.73–2.32) and serous histology. Predictors in endometrial cancerwere lymph node metastases (OR=2.32; 95% CI=1.65–3.26), other distant metastases(OR=1.38; 95% CI=1.08–1.77), high-grade tumors (OR=1.95; 95% CI=1.38–2.76) and clearcell (OR=1.49; 95% CI=1.04–2.13) or serous histology (OR=2.71; 95% CI=2.15–3.42). Incervical cancer, the risk is higher in adenocarcinoma than in squamous cell carcinoma(OR=4.92; 95% CI=3.11–7.79). Conclusion: PM are frequently seen in patients with ovarian cancer. In endometrial andcervical cancer PM are rare. Histological subtype was the strongest predictive factor for PMin all 3 cancers. Better understanding of predictive factors for PM and thus the biologicalbehavior is of paramount importance.
Lara C. Burg,Shenna Verheijen,Ruud L.M. Bekkers,Joanna IntHout,Robert W. Holloway,Salih Taskin,Sarah E. Ferguson,Yu Xue,Antonino Ditto,Glauco Baiocchi,Andrea Papadia,Giorgio Bogani,Alessandro Buda,Roy 대한부인종양학회 2022 Journal of Gynecologic Oncology Vol.33 No.5
Objective: The aim of this study was to assess the SLN detection rate in presumed early stage, low- and intermediate-risk endometrial cancers, the incidence of SLN metastases, and the negative predictive value of SLN mapping performed with indocyanine green (ICG). Methods: A systematic review with meta-analyses was conducted. Study inclusion criteria were A) low- and intermediate-risk endometrial cancer, B) the use of ICG per cervical injection; C) a minimum of twenty included patients per study. To assess the negative predictive value of SLN mapping, D) a subsequent lymphadenectomy was an additional inclusion criterion. Results: Fourteen studies were selected, involving 2,117 patients. The overall and bilateral SLN detection rates were 95.6% (95% confidence interval [CI]=92.4%–97.9%) and 76.5% (95% CI=68.1%–84.0%), respectively. The incidence of SLN metastases was 9.6% (95% CI=5.1%–15.2%) in patients with grade 1–2 endometrial cancer and 11.8% (95% CI=8.1%–16.1%) in patients with grade 1–3 endometrial cancer. The negative predictive value of SLN mapping was 100% (95% CI=98.8%–100%) in studies that included grade 1–2 endometrial cancer and 99.2% (95% CI=97.9%–99.9%) in studies that also included grade 3. Conclusion: SLN mapping with ICG is feasible with a high detection rate and negative predictive value in low- and intermediate-risk endometrial cancers. Given the incidence of SLN metastases is approximately 10% in those patients, SLN mapping may lead to stage shifting with potential therapeutic consequences. Given the high negative predictive value with SLN mapping, routine lymphadenectomy should be omitted in low- and intermediate- risk endometrial cancer.
A Novel Method for the Production and Evaluation of Hernia Repair Mesh in an In Vitro Environment
( Bryant Mersereau ),( Richard E. Groff ),( Timothy Burg ),( Karen J. L. Burg ) 한국조직공학·재생의학회 2012 조직공학과 재생의학 Vol.9 No.3
While most hernia mesh researchers use animal models or patient studies to evaluate prostheses, we have developed an in vitro-centric mesh production and examination system to characterize mesh properties that affect the cellular affinity and treatment potential of repair mesh. A loom was designed and constructed, and a feasibility study was conducted on auto-loom woven, degummed silk mesh seeded with murine-derived D1 multipotent mesenchymal stromal cells (MSCs). Meshes of two different pore sizes, 1 mm and 2 mm, were investigated. The seeded meshes were evaluated using a variety of assays to determine cellular metabolic activity, viability, and conformation at time points of 7, 14, and 21 days. The assays showed substantial cell attachment to the mesh at all time points, including penetration of cells into the interior of silk threads. Metabolic activity peaked at Day 14 for both mesh sizes, with a sharp rise in protein production from Day 7 to 14. Based upon the successful production of mesh and acquisition of results from the assays performed, it can be concluded that an in vitro evaluation system for hernia mesh is both possible and likely to produce scientifically relevant results.
Onder Ozturk,Burge Kabukcu Basay,Ahmet Buber,Omer Basay,Huseyin Alacam,Ali Bacanlı,S˛ enay Görücü Yılmaz,Mehmet Emin Erdal,Hasan Herken,Eyup Sabri Ercan 대한신경정신의학회 2016 PSYCHIATRY INVESTIGATION Vol.13 No.5
ObjectiveaaAttention-deficit hyperactivity disorder (ADHD) is a neurodevelopmental disorder that negatively affects different areas of life. We aimed to evaluate the associations between the Val66Met polymorphism of brain-derived neurotrophic factor (BDNF) and ADHD and to assess the effect of the BDNF polymorphism on the neurocognitive profile and clinical symptomatology in ADHD. MethodsaaTwo hundred one ADHD cases and 99 typically developing subjects (TD) between the ages of 8 and 15 years were involved in the study. All subjects were evaluated using a complete neuropsychological battery, Child Behavior Checklist, the Teacher’s Report Form (TRF) and the DSM-IV Disruptive Behavior Disorders Rating Scale-teacher and parent forms. ResultsaaThe GG genotype was significantly more frequent in the patients with ADHD than in the TD controls, and the GG genotype was also significantly more frequent in the ADHD-combined (ADHD-C) subtype patients than in the TDs. However, there were no significant associations of the BDNF polymorphism with the ADHD subtypes or neurocognitive profiles of the patients. The teacher-assessed hyperactivity and inattention symptom count and the total score were higher, and the appropriately behaving subtest score of the TRF was lower in the GG genotypes than in the GA and AA (i.e., the A-containing) genotypes. ConclusionaaWe found a positive association between the BDNF gene Val66Met polymorphism and ADHD, and this association was observed specifically in the ADHD-C subtype and not the ADHD-predominantly inattentive subtype. Our findings support that the Val66Met polymorphism of BDNF gene might be involved in the pathogenesis of ADHD. Furthermore Val66Met polymorphism of BDNF gene may be more closely associated with hyperactivity rather than inattention.
Acute Dystonia Following a Switch in Treatment from Atomoxetine to Low-dose Aripiprazole
Ömer Başay,Burge Kabukcu Basay,Önder Öztürk,Zeki Yüncü 대한정신약물학회 2016 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.14 No.2
The present report describes the cases of a 17-year-old male patient and a 13-year-old female patient who developed acute dystonia following the administration of low-dose aripiprazole (5 mg/day) after the cessation of atomoxetine treatment. Although aripiprazole-induced dystonia has been previously reported in the literature, it is rare, and most of these cases were associated with doses higher than 5 mg/day. Furthermore, both of the patients in the present study discontinued atomoxetine prior to the initiation of aripiprazole treatment; thus, this report also discussed the possible mechanisms underlying the manifestation of dystonia from the perspective of neurotransmitter activity.