New Evidence of Alleles (V199I and G52S) at the PRKAG3 (RN) Locus Affecting Pork Meat Quality

Chen, J.F.,Dai, L.H.,Peng, J.,Li, J.L.,Zheng, R.,Zuo, B.,Li, F.E.,Liu, M.,Yue, K.,Lei, M.G.,Xiong, Y.Z.,Deng, C.Y.,Jiang, S.W. Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.4

The porcine PRKAG3 (RN) gene encodes the regulatory gamma subunit of adenosine monophosphate-activated protein kinase (AMPK), which is a good candidate gene affecting meat quality. In this study, the effects of two missense mutations A595G (Ile199Val) and G154A (Gly52Ser) in porcine PRKAG3 gene on meat quality traits were studied in M. Longissimus dorsi (LD), M. Semispinalis capitis (SC) and M. Biceps femoris (BF) from different populations of 326 pigs. The PRKAG3 alleles 199I, 199IV, 52S and 52G were identified with PCR-RFLPs and all genotypes - 199I/199I, 199I/199V, 199V/199V, 52S/52S, 52S/52G and 52G/52G - were found. The frequency of V allele was larger than that of I allele in all populations. I allele frequency was zero in Chinese Meishan pigs (population D) especially. G allele frequency was larger than that of S allele in all populations except Large White (population A). Both variations at the PRKAG3 locus significantly affected these meat quality traits. The pork meat quality has not previously been established in Meishan or crosses thereof. The results suggested that generally pH of LD, SC and BF was higher in Meishan pigs than that in other populations. Moreover, Meishan pigs showed higher water-holding capacity and intramuscular fat (IMF), lower water content and water loss percentage compared to other populations in terms of the two variations. The results present here supply new evidence that alleles V199I and G52S at the PRKAG3 locus affect pork meat quality and provide useful information on pork production.

Genetic Variability of mtDNA Sequences in Chinese Native Chicken Breeds

Liu, Z.G.,Lei, C.Z.,Luo, J.,Ding, C.,Chen, G.H.,Chang, H.,Wang, K.H.,Liu, X.X.,Zhang, X.Y.,Xiao, X.J.,Wu, S.L. Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.7

The variability of mtDNA hypervariable segment I (HVS I) sequences was investigated in a total of 48 birds belonging to 12 Chinese native chicken breeds. Sixteen haplotypes were identified from 35 polymorphic nucleotide sites which accounted for 6.4% of a sequenced 544 bp fragment. Diversity analysis of the haplotypes showed that Tibetan, Langshan and Henan cockfight chicken had only one haplotype, while ancient haplotypes existed in Taihe silky and Chahua chicken. Phylogenetic analysis of the haplotypes suggested that Chinese native chicken breeds shared 5 maternal lineages and some breeds would share the same maternal lineage, regardless of their external features and ecological types. Both divergent and phylogenetic analysis of the haplotypes indicated the close genetic relationships between the Chinese native chicken breeds and G. g. gallus and G. g. spadiceus from different areas, which implied that G. g. gallus and G. g. spadiceus were the original ancestors of the Chinese native chicken breeds.

Multilocus Sequence Typing and Virulence Factors Analysis of Escherichia coli O157 Strains in China

Xiao W. Ji,Ya L. Liao,Ye F. Zhu,Hai G. Wang,Ling Gu,Jiang Gu,Chen Dong,Hong L. Ding,Xu H. Mao,Feng C. Zhu,Quan M. Zou 한국미생물학회 2010 The journal of microbiology Vol.48 No.6

Escherichia coli O157:H7, an important food-borne pathogen, has become a major public health concern worldwide. The aim of this study was to investigate the molecular epidemiologic feature of E. coli O157:H7strains in China. 105 E. coli O157:H7 isolates were collected from various hosts and places over 9 years. A multilocus sequence typing scheme (MLST) was applied for bacteria genotyping and polymerase chain reaction (PCR) was used for virulence factor identification. Seven new MLST sequence types (STs), namely ST836, ST837, ST838, ST839, ST840, ST841, and ST842 were identified, which grouped into two lineages. Phylogenetic analysis suggested that the most two frequent STs in China, ST837 and ST836, may be the derivatives of E. coli O157:H7 Sakai or E. coli O157:H7 EDL933. Geographical diversity and host variety of E. coli O157:H7 were observed in China. In addition, the different distribution of tccp was detected. The data presented herein provide new insights into the molecular epidemiologic feature of E. coli O157:H7, and aid in the investigation of the transmission regularity and evolutionary mechanism of E. coli O157:H7.

A two-photon fluorescent probe for specific detection of hydrogen sulfide based on a familiar ESIPT fluorophore bearing AIE characteristics

Chen, L.,Wu, D.,Lim, C.,Kim, D.,Nam, S. J.,Lee, W.,Kim, G.,Kim, H.,Yoon, J. unknown 2017 Chemical communications Vol. No.

<P>Hydrogen sulfide (H2S) is a signaling gasotransmitter, which plays various roles in modulating the functions of different systems. In this study, a 'turn-on'' fluorescent probe for H2S was developed. The probe, whose design is based on a familiar excited state intramolecular proton transfer (ESIPT) fluorophore bearing aggregation-induced emission (AIE) characteristics, was found to exhibit an 80-fold fluorescence enhancement along with a large Stokes shift upon the addition of H2S. Furthermore, the probe was successfully utilized to detect H2S in HeLa cells with both one-photon and two-photon microscopy.</P>

A facile sol-gel method for synthesis of porous Nd-doped TiO₂ monolith with enhanced photocatalytic activity under UV-Vis irradiation

Du, J.,Chen, H.,Yang, H.,Sang, R.,Qian, Y.,Li, Y.,Zhu, G.,Mao, Y.,He, W.,Kang, D.J. Elsevier 2013 Microporous and mesoporous materials Vol.182 No.-

A porous Nd-doped TiO<SUB>2</SUB> monolith was successfully synthesized with polystyrene spheres as a template using a sol-gel method followed by calcination at high temperature. The porous Nd-doped TiO<SUB>2</SUB> monolith was characterized by X-ray diffractometry, scanning electron microscopy and Brunauer-Emmett-Teller measurements. Nd doping can increase the TiO<SUB>2</SUB> surface area by hindering the growth of large TiO<SUB>2</SUB> particles, and suppressing the recombination of photo-produced hole/electron (h<SUP>+</SUP>/e<SUP>-</SUP>) pairs. Moreover, its porous structure can provide a large surface area, facilitating enhanced adsorption and fast transfer of pollutants. To evaluate the photocatalytic activity of the porous Nd-doped TiO<SUB>2</SUB> monolith, the photo-degradation for methyl orange was investigated under UV-Vis irradiation. The porous 0.5% Nd-doped TiO<SUB>2</SUB> monolith showed better behavior than the other as-prepared samples and Degussa P25 due to its narrow band gap, high efficiency for h<SUP>+</SUP>/e<SUP>-</SUP> pair separation, and large surface area. The synergistic combination of Nd doping and the porous structure is a promising material design strategy for use in the degradation of organic pollutants.

A Markov jump model approach to reliable event-triggered retarded dynamic output feedback H_~ control for networked systems

Wang, J.,Chen, M.,Shen, H.,Park, J.H.,Wu, Z.G. Elsevier Science B. V., Amsterdam 2017 NONLINEAR ANALYSIS HYBRID SYSTEMS Vol.26 No.-

In this paper, the problem of reliable event-triggered H<SUB>~</SUB> control is addressed for networked control systems by using retarded dynamic output feedback. The randomness of actuators failures is modeled by a stochastic variable in a Markov jump model framework. To guarantee the considered closed-loop system is stochastically stable with a prescribed H<SUB>~</SUB> performance level, a Markov jump event-triggered retarded dynamic output feedback H<SUB>~</SUB> controller is designed in this paper, which is the main purpose of our study. By using the stochastic analysis techniques and novel integral inequalities, some sufficient conditions for the solvability of the addressed problem are derived. Finally, an example using a satellite control system model is provided to explain the validity of the proposed method.

The Thermal Stability and Elevated Temperature Mechanical Properties of Spray-Deposited $SiC_P$/Al-11.7Fe-1.3V-1.7Si Composite

Hao, L.,He, Y.Q.,Wang, Na,Chen, Z.H.,Chen, Z.G.,Yan, H.G.,Xu, Z.K. The Korean Society for Composite Materials 2009 Advanced composite materials Vol.18 No.4

The thermal stability and elevated temperature mechanical properties of $SiC_P$/Al-11.7Fe-1.3V-1.7Si (Al-11.7Fe-1.3V-1.7Si reinforced with SiC particulates) composites sheets prepared by spray deposition (SD) $\rightarrow$ hot pressing $\rightarrow$ rolling process were investigated. The experimental results showed that the composite possessed high ${\sigma}_b$ (elevated temperature tensile strength), for instance, ${\sigma}_b$ was 315.8 MPa, which was tested at $315^{\circ}C$, meanwhile the figure was 232.6 MPa tested at $400^{\circ}C$, and the elongations were 2.5% and 1.4%, respectively. Furthermore, the composite sheets exhibited excellent thermal stability: the hardness showed no significant decline after annealing at $550^{\circ}C$ for 200 h or at $600^{\circ}C$ for 10 h. The good elevated temperature mechanical properties and excellent thermal stability should mainly be attributed to the formation of spherical ${\alpha}-Al_{12}(Fe,\;V)_3Si$ dispersed phase particulates in the aluminum matrix. Furthermore, the addition of SiC particles into the alloy is another important factor, which the following properties are responsible for. The resultant Si of the reaction between Al matrix and SiC particles diffused into Al matrix can stabilize ${\alpha}-Al_{12}(Fe,\;V)_3Si$ dispersed phase; in addition, the interface (Si layer) improved the wettability of Al/$SiC_P$, hence, elevated the bonding between them. Furthermore, the fine $Al_4C_3$ phase also strengthened the matrix as a dispersion-strengthened phase. Meanwhile, load is transferred from Al matrix to SiC particles, which increased the cooling rate of the melt droplets and improved the solution strengthening and dispersion strengthening.

Investigation of PCR-RFLPs within Major Histocompatibility Complex B-G Genes Using Two Restriction Enzymes in Eight Breeds of Chinese Indigenous Chickens

R. F. Xu,K. Li,G. H. Chen,B. Y. Z. Qiang,D. L. Mo,B. Fan,C. C. Li,M. Yu,M. J. Zhu,T. A. Xiong,B. Liu 아세아·태평양축산학회 2005 Animal Bioscience Vol.18 No.7

New polymorphism of major histocompatibility complex B-G genes was investigated by amplification and digestion of a 401bp fragment including intron 1 and exon 2 using polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP) technique with two restriction enzymes of Msp I and Tas I in eight breeds of Chinese indigenous chickens and one exotic breed. In the fragment region of the gene, three novel single nucleotide polymorphisms (SNPs) were detected at the two restriction sites. We found the transition of two nucleotides of A294G and T295C occurred at Tas I restriction site, and consequently led to a nonsynonymous substitution of asparagine into serine at position 54 within the deduced amino acid sequence of immunoglobulin variableregion- like domain encoded by the exon 2 of B-G gene. It was observed at rare frequency that a single mutation of A294G occurring at the site, also caused an identical substitution of amino acid, asparagine 54-to-serine, to that we described previously. And the transversion of G319C at Msp I site led to a non-synonymous substitution, glutamine 62-to-histidine. The new alleles and allele frequencies identified by the PCR-RFLP method with the two enzymes were characterized, of which the allele A and B frequencies at Msp I and Tas I loci were given disequilibrium distribution either in the eight Chinese local breeds or in the exotic breed. By comparison, allele A at Msp I locus tended to be dominant, while, the allele B at Tas I locus tended to be dominant in all of the breeds analyzed. In Tibetan chickens, the preliminary association analysis revealed that no significant difference was observed between the different genotypes identified at the Msp I and Tas I loci and the laying performance traits, respectively.

Evaluation of mango saponin in broilers: effects on growth performance, carcass characteristics, meat quality and plasma biochemical indices

Y.N. Zhang,J. Wang,B. Qi,S.G. Wu,H.R. Chen,H.Y. Luo,D.J. Yin,F.J. Lu,H.J. Zhang,G.H. Qi 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.8

Objective: The objective of the present study was to determine whether mango saponin (MS) could be used as a feed additive in broiler chicks by evaluating growth performance, carcass characteristics, meat quality, and plasma biochemical indices. Methods: A total of 216 1-d-old Arbor Acres male broiler chicks were randomly assigned into three dietary treatments supplemented with 0 (control), 0.14% (MS 0.14%), or 0.28% (MS 0.28%) MS. Each treatment had six replicates (cages) with 12 chicks each. The feeding trial lasted for six weeks. Results: Compared with the control, dietary supplemented with 0.14% or 0.28% MS increased average daily weight gain of chicks in the grower (22 to 42 d) and the whole (1 to 42 d) phases, and the final body weight of chicks on d 42 was higher in MS supplemented groups (p<0.05). Lower L45 min* (lightness) and L24 h* values, lower b24 h* (yellowness) value, and higher a45 min* (redness) and a24 h* values of the breast muscle were observed in chicks fed with 0.28% MS on d 42 (p<0.05). The total antioxidant capacity in plasma increased in MS 0.14% group on d 21 (p<0.001). Lower contents of plasma total cholesterol and triglyceride were observed in chicks fed with 0.28% MS on d 21 and d 42, whereas the group supplemented with 0.14% MS only decreased plasma triglyceride content on d 21 (p<0.05). The glucose content in plasma decreased in MS 0.28% group on d 42 (p<0.001). Conclusion: Overall, MS could be used as a feed additive in broiler chicks, and the supplemental level of 0.28% MS in diet could improve growth performance, meat quality, and plasma lipid metabolism in broiler chicks.

1997~2000년에 관측된 황사의 농도와 부하량 및 시정 : 위성과 기상 분석 Satellite and Meteorological Analysis

정용승,김학성,박기현,전종갑,첸수젠 한국교원대학교 환경과학연구소 2001 환경연구논문집 Vol.- No.6

This Korea-China study was to monitor the phenomena of yellow sand(YS) occurred during the period of 1997~2000. The analysis on observed data included ground measurements of dust concentration, visibility, satellite imagery, aircraft and lidar observations. In addition, an estimation of atmospheric loadings and study on the relationship between dust concentrations and visibility were carried out. The movement and invasion of YS clouds to Korea were clearly identified with meterological and satellite data. The increasing concentrations of TSP and PM10 agreed well with the satellite information. We define the YS episode with the following values: TSP ≥250, PM10 ≥190 ㎍m-3, and visibility 6.5 km or less. From case studies, we estimated that atmospheric loadings of a dust cloud were over 1 million ton and that the deposition over the Korean Peninsula was in the range of 46,000 and 86,000 ton. For the YS with visibility 3 km, we predict TSP 659 ㎍m-3, PM10 493 ㎍m-3. We recommend the issuance of the YS Watch (advisory) and the YS Warning for the general public. There is a need for the general use of dust collectors in the indoor environment to reduce YS concentrations in spring.