B형 간염 바이러스 단백질에 있어서 HLA-A2에 의해 표현되는 Epitope 펩타이드 들의 분석

이희구,임종석,김승목,이기영,김희수,김승호,권태종,최인성,정태화,김길현 梨花女子大學校 藥學硏究所 1995 藥學硏究論文集 Vol.- No.5

The cytotoxic T lymphocyte (CTL) are an important component in host defense mechanism against viral infection. They can recongnize virus-derived peptides presented by the Class I MHC molecule at the cell surface of the infected cells. On searching for effective CTL epitopes of hepatitis B virus(HBV), we synthesized a distinct set of 9-10 mer peptide containing amino acid sequence of hepatitis B virus surface protein that are selected on the basis of a computer modeling and the previously described HLA-A2 specific motifs.Binding assay of the synthetic peptides to HLA-A2 molecules using human antigen processing defectant T2 cells showed that 3 out of 4 synthetic peptides enhanced the expression of HLA-A2 molecule on T2 cell surface.Two anchor positions, namely P2 and P9(or P10) appeared to play a decisive role for binding.Structural. characteristics of the peptides addressed by molecular dynamics simulation was analysed and compared.These peptides also partially triggered CTL isolated from human peripheral blood mononuclear cells of HBV positive patients, and the response was peptide-spcific.These results showed that negatively-charged amino acid residue at P2 hampered binding affinity of the peptides to HLA-A2 molecules, and that binding affinity of the peptides are not always reflected by their immunogenicity among natural T cell repertoire.

B형 간염 바이러스 단백질에 있어서 HLA-A2에 의해 표현되는 Epitope 펩타이드 들의 분석

이희구,임종석,김승목,이기영,김희수,김승호,권태종,최인성,정태화,김길현 이화여자대학교 생명과학연구소 1995 생명과학연구논문집 Vol.6 No.-

The cytotoxic T lymphocyte(CTL) are an important component in host defense mechanism against viral infection. They can recongnize virus-derived peptides presented by the ClassⅠ MHC molecule at the cell surface of the infected cells. On searching for effective CTL epitopes of hepatitis B virus(HBV), we synthesized a distinct set of 9-10 mer peptide containing amino acid sequence of hepatitis B virus surface proteion that are selected on the basis of a computer modeling and the previously described HLA-A2 specific motifs. Binding assay of the synthetic peptides to HLA-A2 molecules using human antigen processing defectantn T2 cells showed what 3 out of 4 synthetic peptides enhaced the expression of HLA-A2 molemule on T2 cell surface. Two anchor positions, namely P2 and P9(or P10) appeared to play a decisive role for binding. Structural chacteristics of the peptides addressed by molecular dynamics simulation was analysed and compared. These peptides also parially triggerd CTL isolatied frmo human peripheral blood mononuclear cells of HBV positive patients, and the response was peptide-specific. These results showed that negatively-charged amino acid residue at P2 hampered binding affinity of the peptides to HLA-A2 molecules, and that binding affinity of the peptides are not always reflected by thier immunogenicity among natural T cell repertoire.

내시경적 점막절제술로 치험한 식도 과립상 세포종 1례

강혁주,김성욱,최석진,이중현,장재식,서영범,윤병구,박건욱,김성자,김용섭,강승완,이구,양창헌,이창우,김욱년,이광헌,서정일 동국대학교 의학연구소 2000 東國醫學 Vol.7 No.-

과립상 세포종은 Schwann 세포 기원으로 생각되며 인체에 비교적 드물게 발생한다. 과립상 세포종은 전신 어느 곳에서나 발견될 수 있으나 주로 혀, 구강, 피부 혹은 유방 등에서 호발하며 드물게 위장관에서 발견된다. 위장관에서는 식도에서 가장 호발하며 다음으로 위, 대장 순이다. 과립상 세포종은 대부분, 특히 위장관에서는 양성이며 소수의 악성 병변이 보고되었다. 이러한 이유와 함께 수술 전의 진단이 어렵기 때문에 과립상 세포종에 대한 근본적인 치료는 현재까지 외과적 절제술이다. 최근에 시도되는 치료방법들로는 내시경적 레이저 치료, 용종절제술, 내시경적 점막 절제술 등이 있다. 저자들은 상부 소화관 내시경검사를 시행하여 식도 과립상 세포종을 진단하고 내시경적 점막 절제술을 시행하여 합병증 없이 퇴원하여 현재 재발없이 경과 관찰중인 1례를 경험하였기에 보고하는 바이다. Granular cell tumors, which occur infrequently, are probably of Schwann cell origin. They can occur almost anywhere in the body but usually affect the tongue, oral cavity, skin, or breasts and are rarely found in the gastrointestinal tracts. The esophagus is the most frequent gastrointestinal site, followed by the stomach and the colon. Granular cell tumors are generally benign, especially in the gastrointestinal tract, some malignant lesions have been reported. For this reason, and also because preoperative diagnosis is difficult, the standard treatment for granular cell tumor has until now been surgical excision. In recent years, other therapeutic methods is endoscopic laser therapy (ELT), polypectiomy, endoscopic mucosal resection (EMR). We report a case of esophageal granular cell tumor which was diagnosed by an endoscopy and managed using an endoscopic mucosal resection without complication.

전경대원에서 발생한 결핵의 임상 양상

문창기,박상준,조민구,김영중,김소연,김윤권,정준오,안석진,김은실,서승오,김지훈,최원제,이윤영,박형기,최규영,김현근 대한감염학회 2006 감염과 화학요법 Vol.38 No.6

Background: The purpose of this study was to document the incidence and clinical characteristics of patients with tuberculosis (TB) in combat and auxiliary police, living in a group, in Korea where the incidence rate of active TB in a general population is higher than in Western countries. Materials and Methods:We retrospectively reviewed the medical records of all tuberculosis patients diagnosed at National Police Hospital from January 2002 through December 2004. Results:In 2002-2004, a total of 156 cases of tuberculosis were identified with the mean (Standard deviation) age of 20.6 (±1.0) years. Of these, 134 (85.9%) patients were registered as new cases, 11 (7.1%) as relapse, 2 (1.3%) as failure, 5 (3.1%) as treatment-after-default cases whereas 4 (2.6 %) patients were not included in any categories. Average annual new TB rate and smear-positive TB rate were 86.5/10^(5) and 17.4/10^(5) person-years, respectively. In 12 of 31 smear-positive cases, time from onset of symptoms to diagnosis was more than 30 days. Two multidrug-resistance TB cases were identified and two suspected outbreak episodes of TB had occurred during 3 years. Conclusion:There was no statistically significant difference in the incidence rate of new cases of TB between the general population aged 20 to 29 years and combat and auxiliary police in Korea. 배경 : 국내 결핵의 발생은 감소 추세에 있으나 여전히 후진국형 발생양상을 보이고 있다. 특히 집단생활을 하는 젊은이들에서 발생하는 결핵의 유행이 간헐적으로 알려지고 있어 우려를 낳고 있다. 이에 저자들은 최근 전경대원의 결핵 발병 양상에 대해 알아보고자 본 연구를 시행하였다. 재료 및 방법 : 2002년 1월부터 2004년 12월까지 3년간 경찰병원에서 결핵 진료를 받은 전경대원의 의무기록을 후향적으로 분석하여 연도별 결핵 신환발생률, 도말양성결핵 신환율, 진단 지연, 집단발병, 다제내성결핵을 조사하였다. 결과 : 연도별 결핵 신환자 수(10만명당 발생추정치)는 2002년 50명(83.6명), 2003년 42명(83.7명), 2004년 42명(93.5명)이었고 도말양성 폐결핵 신환자 수는 2002년 10명(16.7명), 2003년 11명(21.9명), 2004년 6명(13.4명)이었다. 3년간 도말양성 폐결핵 환자는 31명이 있었고 그 중 12명은 호흡기 증상 발생 30일이 지나서 진단을 받았다. 다제내성 결핵은 2명이 있었으며 결핵의 집단발병이 의심되는 사례는 2차례 있었다. 결론 : 전경대원과 20-29세 연령군 일반인의 결핵 신환발생률, 도말양성 폐결핵 발생률의 통계적으로 유의한 차이는 없었다.

Medicinal Chemistry : EVALUATION OF CADMIUM-INDUCED NEPHROTOXICITY USING URINARY METABOLOMIC PROFILES IN SPRAGUE-DAWLEY MALE RATS

( Yu Kyung Lee ),( Eun Young Park ),( Shi Won Kim ),( Ji Yeon Son ),( Tae Hyung Kim ),( Won Gu Kang ),( Tae Chun Jeong ),( Kyu Bong Kim ),( Seung Jun Kwack ),( Jae Won Lee ),( Suh Mann Kim ),( Byung M 영남대학교 약품개발연구소 2015 영남대학교 약품개발연구소 연구업적집 Vol.25 No.-

The aim of this study was to investigate urinary metabolomics profiles associated with cadmium (Cd)-induced nephrotoxicity and their potential mechanisms. Metabolomic profiles were measured by high-resolution 1H-nuclear magnetic resonance (NMR) spectroscopy in the urine of rats after oral exposure to CdCl2 (1, 5, or 25 mg/kg) for 6 wk. The spectral data were further analyzed by a multivariate analysis to identify specific urinary metabolites. Urinary excretion levels of protein biomarkers were also measured and CdCl2 accumulated dose-dependently in the kidney. High-dose (25 mg/kg) CdCl2 exposure significantly increased serum blood urea nitrogen (BUN), but serum creatinine (sCr) levels were unchanged. High-dose CdCl2 (25 mg/kg) exposure also significantly elevated protein-based urinary biomarkers including osteopontin, monocyte chemoattractant protein-1 (MCP-1), kidney injury molecules-1 (Kim-1), and selenium-bingding protein 1 (SBP1) in rat urine. Under these conditions, six urinary metabolites (citarate, serine, 3-hydroxyisovalerate, 4-hydroxyphentllactate, dimethylamine, and betaine) were involved in mitochondrial energy metabolism. In addition, a few number of amino acids such as glycine, glutamate, tyrosine, proline, or phentlalanine and carbohydrate (glucose) were altered in urine afrer CdCl2 exposure. In particular, the metabolites involved in the glutathione biosynthesis pathway, including cysteine, serine, methionine, and glutamate, were markedly decreased compared to the control. Thus, these metabolites are potential biomarkers for detection of Cd-induced nephrotoxicity. Our results further indicate that redox metabolomics pathways may be associated with Cd-mediated chronic kidney injury. These findings provide a biochemical pathway for better understanding of cellulat mechanism underlying Cd-induced renal injury in hunmans.

NDRG2 is one of novel intrinsic factors for regulation of lL-10 production in human myeloid cell

최승철,김광동,김종태,오상석,윤선영,송은영,이희구,조영경,최인표,임종석,김재화 Sookmyung Women's University Research Institute of 2010 여성과 건강 Vol.6 No.1

배전용변압기의 소음저감을 위한 진동주파수의 스펙트럼분석

김재철,이현구,송승엽,이도훈 崇實大學校 生産技術硏究所 2002 論文集 Vol.32 No.-

This paper analyze spectrum of vibration-frequencies to reduce noise level of d transformer. Using loading back method on same condition of real load to grasp th core and winding which are caused by the transformer vibration. We were mea vibration-frequency by varing appiled voltage and load current of a transformer. cause of a transformer vibration is occured by the harmonic effects, and was identifi vibration is less influenced by the coil. As the result of this paper, comparing the transformer with some isolated materi general transformer, the transformer with some isolated material could reduc amplitude of vibration-frequencies.

칼만증후군(Kallmann's Syndrome) 1례

김용성,백승훈,유경훈,구기선,형근영,김경년,조정구 圓光大學校 醫科學硏究所 1997 圓光醫科學 Vol.13 No.1-2

Kallmann's syndrome is the most common form of isolated gonadotropin deficiency, characterized by hypogonadotropic hypogonadism due to GnRH deficiency, delayed puberty and smelling difficulty. It occurs sporadic or familial pattern, and the mode of inheritence has not been fully documented. The defect in patient of Kallmann's syndrome occurs at suprapituitary level involving mechanism that regulate GnRH synthesis or release, so this syndrome classified as a secondary hypogonadotropic hypogonadism. The gonadotropin or pulsatile GnRH administration enable successful stimulation of spermatogenesis and fertility. We have experienced 1 patient with Kallmann's syndrome and presented with the review of the literature.

초소형 전기장치에 의한 미세 전류가 치아이동에 미치는 효과

김동환,박영국,강승구 대한치과교정학회 2008 대한치과교정학회지 Vol.38 No.5

교정치료 본질인 치아이동을 보다 효과적으로 하기 위한 기계적 힘외의 다른 자극원으로서 전기적 자극에 대한 효용성이 부각되고 있다. 본 연구는 임상에서 초소형 전기장치에 의한 전기 자극이 교정적 치아이동에 미치는 효과를 실증하기 위해 시행되었다. 교정용 미니 스크류를 식립한 성인 여성 교정 환자 7명의 상악 견치 후방 견인 시 실험측에 150 gm의 기계적 교정력 외에 20μA의 직류 전류를 공급하는 초소형 전기 장치를 고안, 제작, 장착하여 견치의 근원심에 1일 5시간씩 4주간 전류 자극을 흘리고, 대조측에는 기계적 자극만을 가한 후, 매 주간 치아 이동량을 측정하여 치아이동에 있어서 전기자극의 효과를 검증하였다. 실험기간 동안 치아이동률에 있어서 실험군에서 대조군에 비해 1, 2째주에서 33%, 3, 4째주에서 21.4% 증가된 치아이동을 보였다. 실험군의 평균 치아 이동량은 1, 2째주가 3, 4째주에 비하여 컸으며, 그 차이는 각각 47%, 24% 증가된 치아 이동량을 보였다. 실험을 통하여 미세 전류가 적용되어 치아이동을 촉진할 수 있다는 것이 임상적으로 실증되었다. 생물학적으로 최적의 치아이동을 계획하여 치료계획을 단축시킬 수 있는 방법은 기계적 교정력과 전기자극을 동시에 가하는 것이라고 제안한다. Objective: The purpose of this study was to determine whether an exogenous electric current to the alveolar bone surrounding a tooth being orthodontically treated can enhance tooth movement in human and to verify the effect of electric currents on tooth movement in a clinical aspect. Methods: This study was performed on 7 female orthodontic patients. The electric appliance was set in the maxilla to provide a direct electric current of 20μA. The maxillary canine on one side was assigned as the experimental side, and the other as control. The experimental canine was provided with orthodontic force and electric current. The control side was given orthodontic force only. Electrical current was applied to experimental canines for 5 hours a day. The amount of canine movement was measured with an electronic caliper every week. Results: The amount of orthodontic tooth movement in the experimental side during 4 weeks was greater by 30% compared to that of the control side. The amount of increase in tooth movement in the experimental side was statistically significant. The amount of tooth movement in the experimental side during the first two weeks was greater than that in the following two weeks. The amount of weekly tooth movement in the control side was decreased gradually. Conclusions: These results suggested that the exogenous electric current from the miniature electric device might accelerate orthodontic tooth movement by one third and have the potential to reduce orthodontic treatment duration.

선형회귀모형에서 다수이상치 탐색 방법에 관한 연구

김승구,송민구 尙志大學校 1998 論文集 Vol.19 No.-

We consider the problem of identifying and testing multiple outlier in weighted linear regression models. When case wieights are given because of variance heterogeneity, we can estimate efficiently regression parameters using weighted least squares estimators are sensitive to outliers like ordinary least squares estimations. Thus, in this paper, We propose some statistics for regression diagnostics in weighted least square regression. On the other hand, we introduce testing procedures for the detection of multiple outliers that appear to be less sensitive to this problem. Both procedures attempt to separate the data into a set of "Clean" data points and a set of points that contain the potential outliers. The potential outliers are then tested to see how extreme they are relative to the clean subset, using an appropriately scaled version of the prediction error. As the outlier-detection methods of H.S(HadiandSimonoff), in this study, we would like to suggest that we uses σ² which definite of back and forth is trimmed instead of σ² is used on the second step of H.S method after arranging γ², in order.