전립선 기질세포의 증식과 COX-2 발현에 대한 프로게스테론의 영향

정수련,김성한,최이화,박지은,전은미,강영진,이광윤,최형철 영남대학교 의과대학 2006 Yeungnam University Journal of Medicine Vol.23 No.1

전립선비대증은 노인 남성에서 흔히 유발되는 질환이며, 노화가 진행될 수록 빈도가 높아지는 특징을 가진다. 이 질환의 원인은 전립선기질세표의 과도한 증식으로 유발된다고 알려져 있지만 그 자세한 기전에 대해서는 잘 알려져 있지 않다. 전립선비대증에서 progesterone 수용체 양성 세포가 다른 전립선 종양에 비해서 많고, progesterone은 testosterone에서 DHT로 전환되는 것을 감소시키는 역할을 가진다고 알려졋다. 또한 남성 전립선 평활근의 과증식에 의한 질환이므로 평활근 세포의 증식과 관련성이 있다고 보고된 COX-2의 전립선비대증에 대한 영향에 대한 연구가 필요하다. 전립선 기질세포에 progesterone을 3일간 투여하여 배양한 경우 기질세포 증식은 차이가 없었다. Progesterone을 단독 또는 DHT와 같이 투여한 기질세포에서 남성호르몬 수용체 mRNA 발현은 비처리군과 비교하여 유의한 차이가 없었다. 또한 progesterone과 DHT 동시 투여에 의한 COX-2 mRNA 발현에도 차이가 없었다. 그러나 progesterone에 의한 남성 호르몬 수용체와 COX-2 단백 발현에서는 대조군과 비교하여 유의하게 감소시켰다. 이상의 결과는 progesterone은 남성호르몬 수용체에 대해 전사 후 반응 (post-transcriptional response)에 효과를 나타내어 남성호르몬 수용체 발현을 감소시키는 작용은 가지며, COX-2 발현 억제효과를 나타내므로 전립선비대증의 치료에 이용될 수 있을 것으로 사료된다. Background: Benign prostatic hyperplasia (BPH) is the most common benign tumor in older men; the etiology of this disease remains poorly understood. Testosterone and dihydrotestosterone (DHT) both act as androgen via a single androgen receptor. Testosterone is converted to DHT by 5α-reductase in prostatic stromal cells. Progesterone has been reported to inhibit DHT conversion; howevwe, its effect on prostatic stromal cells remains to be elucidated. Materials and Methods: In this experiment, we investigated the effect of progesterone on androgen receptor expression induced by DHT. We also tested the effect of progesterone on cyclooxygenase-2 (COX-2) expression, as well as prostate stromal cell proliferation using the cell count kit-8. Results: Progesterone did not cause an increase of prostate stromal cell proliferation. The mRNA expression of the androgen receptor and COX-2 were not changed by progesterone; the expressions of androgen receptor and COX-2 proteins were decreased by progesterone in prostate stromal cells. Conclusion: These results suggest that in prostate stromal cells, progesterone decreases androgen receptor protein expression, which results in decrement of COX-2 protein expression. This effect might be mediated by post-transcriptional regulation.

減壓貯藏이 Tomato果實의 着色에 미치는 영향

趙來光,崔鍾旭,孫泰華 COLLEGE OF AGRICULTURE KYUNGPOOK NATIONAL UNIVERSI 1984 慶北大農學誌 Vol.2 No.-

This experiment was conducted to observe the effect of subatmospheric pressure storage on the coloration of tomato fruits. Tomato fruits(Lycopersicon esculentum Kang Lyuk Misu), which were cultured on field, were havested at the mature green stage on June 16, 1976 and stored 10℃ under normal atmospheric pressure (NAP) and sub-atmospheric pressure (SAP). Furthermore, the treatment at SAP was subdivided into CO_(2) 0%(SAP-O), CO_(2) 5% (SAP-5) and CO_(2) 10%(SAP-10) concentration, respectively. The results obtained were as follows : The chlorophyll content was rapidly decreased for 10 days, whereas the content of chlorophyll in SAP treatment was retained 2㎎% after 40 days. The lycopene content in NAP-5 was 5㎎% after 20 days. On the other hand, that in SAP-10 was 3㎎% art after 40 days. The red color development was retarded for 30 days in SAP-10. Comparing with the formation of lycopene, that of carotene was less inhibited by storage conditions (carbon dioxide, temperature, pressure) and increased more slightly in SAP than in NAP. The ripening of tomato fruits in NAP was retarded for about 30 days as compared that in SAP. The intensity of respiration in SAP was weaker than that in NAP. Also the peak of respiration in SAP was delayed compared to that of NAP. The content of reducing sugar and titratable acidity in SAP were retained more than in NAP up to 30 days tested.

학교 주5일제에 관한 학생과 교사의 의식 분석

홍광식,최영자,이연화,정정임,장남덕 한국초등교육학회 2002 초등교육연구 Vol.15 No.1

본 연구는 학교 주5일제에 관한 학생과 교사의 의식을 조사분석하여 학교 주5일제 실시에 대응하고자 하였다. 조사 대상자는 초·중·고 학생 1558명과 교사 1077명이었다. 연구결과, 학생들은 현재 학교생활에서 공부하는 즐거움을 알지 못하고 친구들과의 교제에서 즐거움을 찾고 있는 것으로 나타났다. 학교 주5일제 관해서는 학생과 교사 대부분이 찬성하고 있으며, 찬성 이유는 여유있는 하루, 가족과 접촉시간을 갖고 취미활동을 하며 친구와 놀 수 있기 때문이라고 하였다. 교사들은 학교 주5일제로 인한 수업시수의 문제를 감축해도 상관이 없다는 반응이 많았고, 현재의 수업시수를 확보해야 한다는 의견은 적었다. 학생과 교사 대부분은 학교 주5일제는 학생들이 다양한 체험을 할 수 있는 기회를 제공하는 체제로 인식, 긍정적인 반응을 보였다. The purpose of this study is twofold: (ⅰ) to analyze students and teachers' consciousness on the proposed Five-day-work-week School System(FSS) plan; and (ⅱ) to propose a desirable direction of school management including curriculum compatible with the (FSS) plan. This study developed as follows. For the theoretical background of this study, previous research was reviewed. For the items of the questionnaire, the Japanese Ministry of Education Survey on the FSS at school was referred to. The Subjects sampled were 1558 students and 1077 teachers, The data collected from the at elementary, secondary and high school of students and teachers were statistically analyzed by using the SPSS WIN 7.5. The following are the results of the survey. First, in order to adopt the FSS plan, schools have to develop new programs connecting home and school. It is required that school develop special programs that the local community can participate in and cooperate with. Second, most of the respondents support the FSS plan because students can have more chances to be with their family, to become familiar with nature, and to attend various activities sponsored by the local organizations. Third, to argue that it is desirable to adopt the FSS plan even though school has to reduce the minimum class hours. The reason they are against the no-school-on-saturday plan is that there are many families who cannot take care of their children on Saturdays; thus, special programs for them should be prepared before the plan is put into practice. It is also required that we develop various sports or culture events for young adolescents and make up more organizations for youth. We should have school or community facilities open to young adolescents and children whose parents are unavailable to care for them. The latter should have an easy access to those facilities. From these observations, we can conclude that it is imminent to develop various programs before we put the FSS plan into practice. Schools should cooperate fully with homes and the community.

정신지체아와 학습부진아의 작업기억 특성에 관한 비교연구

여광웅,김나영,정영숙,이태화 한국정신지체아교육학회 2001 지적장애연구 Vol.3 No.-

이 연구는 정신지체아와 학습부진아의 작업기억(working memory) 특성을 비교 분석하여, 그들의 인지능력 즉 정보처리 용량에 관련된 변인과 이에 따른 교수방법을 탐색 논의하였다. 연구결과와 논의를 통해 볼 때, 학습부진아의 작업기억 용량이 정신지체아보다 더 큰 것으로 나타났으며, 모두 지능과 연령 그리고 자극 제시시간변인이 기억탐색 속도, 전략사용, 부호화 등의 다양한 심리적 기제를 통해 작업기억 용량에 영향을 주고 있는 것으로 밝혀졌다. 그리고 정신연령과 자극 제시시간이 증가할수록 작업기억 용량이 증가되는 것으로 나타났으며, 이것은 정신연령과 자극 제시시간이 증가함에 따라 기억 수행에 용이한 심릭적 기제가 활성화 되고 있음을 의미한다. The present study was designed to define the capacity of working memory (WM) of mentally retarded(MR) and Underachiever(UA). Subjects were 30 MR and 30 UA who were the same mental age, sampled from a special classes in the elementary schools and the middle schools in chungnam. Two group was formed according to three mental age (6,8,10) IQ scores ranges 50~75 for MR group, 90~110 for UA group. In order to define the capacity of WM of MR and UA, group(MR, UA), mental age(6,8,10) and speed of task presentation (3", 6")were used as independent variables and the capacity of WM as dependent variables. This study was taken following statistical methods in order to test study problems, problem 1 was conducted three-way analysis of variance by repeated-measures. Turky test was conducted to notice difference according to age. The main conclusion were as follows. First, the capacity of WM of UA is more larger than that of MR. Second, there are significant differences in the capacity of WM of MR according to mental age and speed of task presentation. Again as mental age and speed of task presentation in crease, the capacity of WM of MR increases. Finally, the capacity of WM of UA increases according to mental age and speed of task presentation. Jst as with MR, as mental age and speed of task presentation increase, the capacity of WM of UA increases.

결핵균 30 kDa 항원과 Triton X-100 Solubilized Protein 항원에 의한 대장암 주변 림프절 단핵구의 활성화

박정규,김광호,조은경,임재현,민들레,송영자,김화중,백태현 忠南大學校 癌共同硏究所 1998 癌共同硏究所 硏究誌 Vol.2 No.1

Tumor-draining lymph node mononuclear (TDLMN) cells are specifically sensitized to the growing tumor but such cells are deficient for mediating an antitumor response. In this study, we examined the feasibility of using mycobacterial 30 kDa or Triton X-100 solubilized protein (TSP) antigen to stimulate mononuclear cells of colon cancer-draining lymph node for the generation of cell mediated immune effector cells. The proliferative response of TDLMN cells stimulated with mycobacterial 30 kDa or TSP antigen was determined by ^(3)H-thymidine incorporation assay. The proliferation of TDLMN cells to mycobacterial 30 kDa or TSP antigen was significantly increased in PPD (+) patients, but a poor response to the 30 kDa or TSP antigen was observed in PPD (-). The expression on γδ T cells to mycobacterial 30 kDa or TSP antigen was assessed by flow cytometry. The γδ T cells from PPD ( + ) patient responded only to 30 kDa antigen but to TSP antigen. An investigation of cytokine mRNA expression was undertaken using reverse transcription-polymerase chain reaction (RT-PCR) to follow TDLMN cells stimulated with the 30 kDa or TSP antigens for 5 days. The IFN-γ and TNF-α mRNA expression was only induced in TDLMN cells of PPD ( + ) patient in response to the 30 kDa or TSP antigen. The IL-2 mRNA expression was induced in both PPD (+) and PPD (-) in response to the 30 kDa or TSP antigen. But the IL-4 mRNA expression was not induced in response to the 30 kDa or TSP antigen. These results suggest that the 30 kDa and TSP antigens may serve as biologic response modifier for the generation of cell mediated immune effector cells.

<i>In vitro</i> and <i>in vivo</i> imaging and tracking of intestinal organoids from human induced pluripotent stem cells

Jung, Kwang Bo,Lee, Hana,Son, Ye Seul,Lee, Ji Hye,Cho, Hyun-Soo,Lee, Mi-Ok,Oh, Jung-Hwa,Lee, Jaemin,Kim, Seokho,Jung, Cho-Rok,Kim, Janghwan,Son, Mi-Young Federation of American Societies for Experimental 2018 The FASEB Journal Vol. No.

<P>Human intestinal organoids (hIOs) derived from human pluripotent stem cells (hPSCs) have immense potential as a source of intestines. Therefore, an efficient system is needed for visualizing the stage of intestinal differentiation and further identifying hIOs derived from hPSCs. Here, 2 fluorescent biosensors were developed based on human induced pluripotent stem cell (hiPSC) lines that stably expressed fluorescent reporters driven by intestine-specific gene promoters Kruppel-like factor 5 monomeric Cherry (KLF5(mCherry)) and intestine-specific homeobox enhanced green fluorescence protein (ISXeGFP). Then hIOs were efficiently induced from those transgenic hiPSC lines in which mCherry- or eGFP-expressing cells, which appeared during differentiation, could be identified in intact living cells in real time. Reporter gene expression had no adverse effects on differentiation into hIOs and proliferation. Using our reporter system to screen for hIO differentiation factors, we identified DMH1 as an efficient substitute for Noggin. Transplanted hIOs under the kidney capsule were tracked with fluorescence imaging(FLI) and confirmed histologically. After orthotopic transplantation, the localization of the hIOs in the small intestine could be accurately visualized using FLI. Our study establishes a selective system for monitoring the in vitro differentiation and for tracking the in vivo localization of hIOs and contributes to further improvement of cell-based therapies and preclinical screenings in the intestinal field.</P>

Activation of AMPK by <i> Buddleja officinalis</i> Maxim. Flower Extract Contributes to Protecting Hepatocytes from Oxidative Stress

Jung, Ji Yun,Lee, Chul Won,Park, Sang Mi,Jegal, Kyung Hwan,Kim, Jae Kwang,Park, Chung A.,Cho, Il Je,Jung, Dae Hwa,An, Won G.,Ku, Sae Kwang,Zhao, Rongjie,Kim, Sang Chan Hindawi 2017 Evidence-based Complementary and Alternative Medic Vol.2017 No.-

<P>The<I> Buddleja officinalis </I>Maxim. flower is used in traditional Chinese and Korean medicine to treat inflammation, vascular diseases, headache, and stroke, as well as enhance liver function. This research investigated the effects of<I> B. officinalis</I> Maxim. flower extract (BFE) on hepatotoxicity. The cytoprotective effects and mechanism of BFE against severe mitochondrial dysfunction and H<SUB>2</SUB>O<SUB>2</SUB> production in hepatotoxicity induced by coadministration of arachidonic acid (AA) and iron were observed in the HepG2 cell line. In addition, we performed blood biochemical, histopathological, and histomorphometric analyses of mice with carbon tetrachloride- (CCl<SUB>4</SUB>-) induced acute liver damage. BFE inhibited the AA + iron-mediated hepatotoxicity of HepG2 cells. Moreover, it inhibited mitochondrial dysfunction, H<SUB>2</SUB>O<SUB>2</SUB> production, and glutathione depletion mediated by AA + iron in the same cells. Meanwhile, the cytoprotective effects of BFE against oxidative stress were associated with the activation of AMP-activated protein kinase (AMPK). In particular, based on the histopathological observations, BFE (30 and 100 mg/kg) showed clear hepatoprotective effects against CCl<SUB>4</SUB>-induced acute hepatic damage. Furthermore, it inhibited 4-hydroxynonenal and nitrotyrosine immunoreactivity in hepatocytes. These results provide evidence that BFE has beneficial hepatoprotective effects against hepatic damage via the activation of AMPK pathway. Accordingly, BFE may have therapeutic potential for diverse liver disorders. </P>

A New Cold Tolerant, High Forage and Grain Yielding Winter Oat Cultivar “Samhan”

Hwa-Young Heo,Hyong-Ho Park,Maing-Jung Kim,Sun-Woo Choi,Kwang-Geun Park,Jung-Hyun Nam,Jung-Gon Kim,Choon-Ki Lee,Young-Up Kwon 한국육종학회 2003 한국육종학회지 Vol.35 No.4

Samhan (Avena sativa L.), a winter forage oat was released by the National Crop Experiment Station (NCES) in2001. Samhan was selected from the cross Early 80/CI 7518. CI 7518 is a winter oat line that was obtained from UnitedStates Department of Agriculture (USDA). Subsequent generations followed by the cross were handled in bulk and pedigree selec-Suwon, and was designated as a line name of Gwiri 37. Samhan was subsequently evaluated for winter hardiness and forageyield in Suwon, Unbong and Iksan from 2000 to 2001. Over 2 years, the average forage yield of Samhan harvested at May 10was 7.1 ton ha1, compared with 2.76 ton ha1 for Ol and 2.96 ton ha1 for Swan. Samhan is about 6 days later than Olfor heading date. Samhan is adapted primarily for winter planting for forage use in the central regions of Republic of Korea.

A New Cold Tolerant, Lodging Resistant and High Grain Yielding Forage Triticale Cultivar “Shinyoung”

Kwang-Geun Park,Hwa-Young Heo,Hyoung-Ho Park,Maing-Jung Kim,Jung-Hyun Nam,Choon-Ki Lee,Jung-Gon Kim,Young-Up Kwon 한국육종학회 2003 한국육종학회지 Vol.35 No.4

Shinyoung triticale (X Triticosecale Wittmack), a winter forage triticale was released by the National Crop Exper-1987. Subsequent generations were handled in pedigree selection program at Suwon and Yeoncheon from 1989 to 1995 by NCES.A head row was selected for winter hardiness and agronomic appearance in 1995 and designated SWT1967-B-9-8-7-9-3 forpreliminary yield trial. In 1998, it was designated Suwon 25 and placed in regional yield trials. Over 3 years (1999~2001),Shinyoung averaged 5.92 ton ha1check variety Paldang as a result of late heading. But it yielded 4.29 ton ha1 of grain yield and was superior to other varietieswith increase of 25% more than Paldang and 23% more than Koolgrazer. The heading date of Shinyoung was May 12which was 9 days later than that of Koolgrazer. It would be recomended as a winter forage crop when harvested at headingstage (around May 10) in South Korea.

A New Cold Tolerant, Lodging Resistant and High Grain Yielding Forage Triticale Cultivar "Shinyoung"

Kwang Geun Park,Hwa Young Heo,Hyoung Ho Park,Maing Jung Kim,Jung Hyun Nam,Choon Ki Lee,Jung Gon Kim,Young Up Kwon 한국육종학회 2003 한국육종학회지 Vol.35 No.5