家蠶으로부터 分離된 새로운 Microsporidia S80의 特性

林鍾聲,趙世衍 COLLEGE OF AGRICULTURE KYUNGPOOK NATIONAL UNIVERSI 1983 慶北大農學誌 Vol.1 No.-

The new microsporidia S80 isolated from, Bombyx mori L. in Korea showed ovoid in the morphology of the spores and the size were measured 2.9±0.28μ in length and 1.7±0.29μ width. No other microsporidian spore like this has not been so far isolated from Silkworm. The length of the polar filament extruded in hydrogen peroxide (H_(2)O_(2)) at 30℃ was 26μ of a round cytoplasm on the top. The spores were partly stained with Giemsa, Safranin-O and Gram as the same staining properties as Nosema bombycis, Microsporidia K 79 and other microsporidian spores. The fine structures were observed under scanning eleceron microscope through ultrathin sectioning. The spore wall was composed of three layers ; the thin exospore of an electron dense rippled layer, the thick electron lucent endospore which was thinning considerably at the polar filament insertion point, and the inner limiting membrane. Polar cap present at the sporeapex, with a long polar filament of 12-13 coils, subtending angle of 60˚ to spore axis, which is tubular made up of a multilayered and are a benes core, light ring structure enclosing the dance core, the dark ring structure enclosing the inner light ring structure and the other than and light ring structure bounded from cytoplasm. Lamellate polaroplast occupied the anterior part of the spore, and the two neclei with dense nucleoplasm bounded by a double nuclear envelope were cited in the slight downer middle portion of spore. From the characteristics of the shape, size and fine structures, it is certain to reason the Microsporidia S80 belong to the phylum Microspora, class Microspora, order Microsporida. order Microsporida. The shape of two nuclei cited seems to be genus Nosema, but in the classification for the suborder it should be defined wheather pansporoblasts be formed or not and for the genis especial attempts have been made to define the characters which distinguish the disporous genera in the life cycle. Survey through the infection of the bad cocoons during 1980 to 1982 in South Korea the areas contaminated with new microsporidia were revealed 5 provinces of Kyung-Gi, Kang-Won, Chung-Nam and Chun-Nam. Pathological effects inoculated per os at second instar larvae of silkworm, the LD 50 was 7.1×10^(7)/ml as lower pathogenecity than that of Nosema bombycis Naegeli of 1.2×10_(7)/ml. While on the other hand the inoculation of the microsporidia at fourth instar larvae lowerd the whole cocoon weight and cocoon shell wegith and significant at 1% level. The microsporidia S80 defined it can not be transmitted transovarially from the result of predictive and collective examination of 21 egg barches from the infected female moth.

Aspirin投與가 Rat의 胃粘膜 細胞에 미치는 微細形態學的硏究

張仁浩 COLLEGE OF AGRICULTURE KYUNGPOOK NATIONAL UNIVERSI 1983 慶北大農學誌 Vol.1 No.-

A study was undertaken on rats in order to clarify the gastric mucosal, morphologic response to oral and subcutaneous administration of acetylsalicylic acid(aspirin). Aspirin was administered orally (oral group) and subcutaneously (subcutaneous group) at does rate of 60 mg per kg of body weight per day to 40 normal rats. On the 1st, 3rd, 7th, and 15th day of administration, in addition to clinical observation, 5 rats each from the both groups were sacrificed and examined macroscopically, histologically and electron-microscopically for the morphological changes of gastric mucosal cells with the following results. Although the clinical, macroscopic and histological changes were not significant, marked ultrastrastural changes were observed. Parietal and chief cells were affected most severely by the administration of aspirin ; parietal cells showed increase in the number of SER and intracellular canaliculi, where-as in chief cells fragmentation, luminal dilatation, decrease in the number and structural abnormalities of RER were seen. Relatively mild changes were observed in mucous, mucous neck and basal-granulated cells. Although the degree of changes was milder than those of oral group, the similar changes were also observed in the subcutaneous group. From these results, it would be concluded that aspirin injury of gastric mucosa is effected not only by the direct injury to the mucosa but also indirectly by the blood concentration of arpirin.

春播時期가 마늘의 球形成 및 收量에 미치는 影響

李愚升,李明喜 COLLEGE OF AGRICULTURE KYUNGPOOK NATIONAL UNIVERSI 1984 慶北大農學誌 Vol.2 No.-

This study was conducted to investigate the effect of planting date and size of seed cloves on bulb formation and yield in spring plantings of garlic. A split plot design with five replications was used. The main effects were 6 planting dates: at intervals of 10 days from March 5 to April 25, 1979. The subplots were 6 size groups of seed cloves: at intervals of 0.5g from 1.5 to 4.5g. The results obtained are summarized as follows: 1. Number of cloves per bulb was decreased as planting was delayed. The number of cloves was markedly decreased in the plots planted after April 16. The effect of size of seed cloves on the number of cloves was not significant. 2. Weight of cloves per plant was also decreased as planting was delayed. The weight of cloves per plant was markedly decreased in the plots planted on or after April 16. The weights of cloves both in the outer and inner layers were also decreased as planting was delayed. However, the inner layer cloves were significantly heavier than outer layer ones in the plots planted on or after April 16. 3. When considered the distribution of number and weight of cloves per bulb as influenced by planting date, relatively large cloves weighing 3 to 4g were frequent in the bulbs produced in the plots planted on or before April 4, while relatively small cloves weighing 1 to 2g were common in the plots planted thereafter.

딸기에 있어서 뿌리썩이 線? (Pratylenchus vulnus) 防除를 위한 土壤 燻蒸劑 處理 ?果에 關한 硏究

崔永然,金東根 COLLEGE OF AGRICULTURE KYUNGPOOK NATIONAL UNIVERSI 1984 慶北大農學誌 Vol.2 No.-

In order to control the root-lesion nematode, Pratylenchus vulnus on strawberry, the effects of six soil fumigants; Cylone, Dowfume MC-2, Di-Trapex, D-D, Telone C-17 and Telone Ⅱ, were evaluated in the farmer's field in 1982 and 1983 in Korea. The number of nematodes were reduced in plots treated with fumigants and the number of leaves and total fresh weight of strawberry were superior to untreated control plots. The treatments of Dowfume Mc-2 at 50㎏/10a and Telone C-17 at 10ℓ/10a increased the yields of strawberry by 38 and 26%, respectively, compared with untreated control plots in 1982. In 1983, the number of nematodes were maintained at considerably low on 180 days after applications. However, on 270 days after the fumigants applications, the number of nematodes in treated plots resumed the similar levels as that before the treatment of chemicals. These values were less than those of untreated control plots investigated on 270 days after. The treatments of Cylone at 30ℓ/10a and Di-Trapex at 30ℓ/10a gave the best results, with increases in yield of 21 and 16%, respectively, compared with untreated control plots. The treatments of Dowfume MC-2 at 50㎏/10a, D-D at 50ℓ/10a, Telone C-17 at 10ℓ/10a and Telone Ⅱ at 8.4ℓ/10a increased the yields by 14, 13, 12 and 10%, respectively.

Liatris spicata 에 있어서 球의 크기가 生育, 開花 및 球發育에 미치는 影響

Suh, Young-Kyo,Choi, Sang-Tai COLLEGE OF AGRICULTURE KYUNGPOOK NATIONAL UNIVERSI 1984 慶北大農學誌 Vol.2 No.-

This study was conducted in order to investigate the effect of seperated corm size on the growth, flowering and corm development after planting in Liatris spicata. Nine grades of corm with one main bud (NC), weighing 1.0-9.9g, and six grades of corm with several main bud(OC+NC), weighing 1.0-15.9g, were investigated. The results of this study were summarized as follows: The period from planting to germination was 13.9-22.9 days, and percentage of germinated buds in OC+NC was 70-80%. The period from planting to flowering was 102.4-117.2 days. The larger the corm size, the higher the percentage of flowering corm. The percentage of flowering corm was 100% when the corm weight was above 4g in NC and 14g in OC+NC, and the percentage of nonflowering corm was above 40% when the corm weight was below 8g in OC+NC. The lager the corm size, the higher the quality of flower evaluated by plant height, spike length and floret number, but when the corm weight was below 10g in NC and 16g in OC+NC, they could not produce excellent cut flower. Corm development was better in NC than OC+NC. Increasing rate of corm weight was about 5-9 times in NC, while about 3 times in OC+NC. Number of main buds of produced corm was about 3-7, and increasing rate of that was 1-2 times. The larger the separated corm size, the higher the quality of produced corm evaluated by corm weight and number of main bud. Therefore, in order to produce the excellent corm for next season's culture, it was belived that the separated corm weight should be heavier than 5g in NC and 12g in OC+NC.

人工飼料育과 桑葉育에 있어서 核多角俸 바이러스에 對한 누에의 品種別 抵抗性

林鍾聲,崔鍾九 COLLEGE OF AGRICULTURE KYUNGPOOK NATIONAL UNIVERSI 1984 慶北大農學誌 Vol.2 No.-

This study was carried out to investigate the resistance of the silkworm larvae of various varieties fed on artificial diet and mulberry leaves to Nuclear Polyhedrosis Virus. The results obtained were as follows: In the oral inoculation of Nuclear Polyhedrosis Virus to the silkworm larvae, high mortality was observed in the highest concentration of inclusion bodies (10^(8)/㎖) to all the different varieties of silkworm larvae such as Jam 107 x Jam 108, Jam 108 x Jam 107, Jam 117 x Jam 118, Daizo and Jam 129 x Jam 119. The susceptility of silkworm larvae at 2nd instar to the oral inoculation of N. P. V. was higher than that of the larvae at 4th instar in any kinds of varieties. The Daizo was proved to be most resistant to the N. P. V. inoculation throughout all the different instar and both in mulberry leaves and artificial diet while Jam 120 x Jam 119 to be most susceptible in the same conditions as above. However, Jam 107 x Jam 108 and Jam 108 x Jam 107 were in the same level of the susceptibility to the inoculation. In the histopathological diagnosis, it was examined that fat body was more susceptible to the N. P. V. inoculation at 2nd and 4th instar. After infection, the gastric juices were sampled to carry out electrophoresis to make a comparison of bands. However, there was no difference in numbers except a little increase of concentration. It was also noticed that various bands disappeared as the disease is progressed.

사과酒에서 分離한 産膜酵母에 關한 硏究 : (1) 菌株의 分離 및 同定 (Ⅰ) Isolation and Identification of Yeast Strain

鄭基澤,宋亨翼 COLLEGE OF AGRICULTURE KYUNGPOOK NATIONAL UNIVERSI 1983 慶北大農學誌 Vol.1 No.-

Film-formation, which often occurs during storage of apple wine, owing to contamination by film-forming yeasts, results in inferior products. Therefore, for the purpose of preventing this occurrence, we isolated and identified yeast strain. Among the total number of 45 yeast strains which were isolated from contaminated apple wine in winery, the strains FY-4 and FY-5 were found to be useful. The strain FY-5, which greatly formed film on apple wine, was identified as Hansenula beijerinckii or similar strain according to taxonomic characteristics.

쥐의 대뇌동맥윤에 관한 연구

원봉래,이재현 COLLEGE OF AGRICULTURE KYUNGPOOK NATIONAL UNIVERSI 1984 慶北大農學誌 Vol.2 No.-

Little has been reported concerning circulus arterious cerebri in adult rats. Certain morphological features not described previously were observed. The more significant of results these were as follows: The asymmetrical origin of the posterior cerebral artery was found to occur in more than half of the animals. Presence of cleft-like buttonhole formations along an anterior cerebral artery at one side or both sides was a feature that show practically the species characteristic. The supplemental anterior communicating artery was occasionally found at the place of fusioning with the two anterior cerebral arteries. Exceptionally, 3 cases of the anterior cerebral artery did not fuse to both arteries but they were unusually connected with the two anterior communicating arteries. An olfactory artery to the nasal cavity was prominently branched from the anterior cerebral artery in all animals.

貯藏中 사과의 Polyphenol Oxidase의 變化에 對하여

金玉淵,孫泰華 COLLEGE OF AGRICULTURE KYUNGPOOK NATIONAL UNIVERSI 1983 慶北大農學誌 Vol.1 No.-

This report decribes the changes of internal browning and PPO in apples, when the method of storage was changed in the condition of low temperature. Internal browning was found more Fuji harvested in November than Fuji in October. The degree of internal browning was the highest during the storage of 0.09mm 2% and 0.06mm 2%, 0.06mm 20% was followed. In the subatmospheric storage with complete remove of CO_(2) internal browning was not observed. During the storage of P.E. film bag, Fuji havested in November was higher than in October, and Fuji on 0.09mm 2% was higher than in 0.06mm, and Fuji during the storage of P.E. film bag was increased in PPO activity and PPO specific activity. During the subatmospheric storage with complete remove of CO_(2), PPO activity of Fuji was little changed, but it's PPO specific activity was continuously decreased. PPO activity of Ralls was more higher than PPO activity of Fuji. PPO activity and PPO specific activity during the early stage of storage and the end stage of storage were little changed. Internal browning of Fuji was more effected CO_(2) injury than chilling injury. There is no relation between internal browning and degree of PPO activity was effected by CO_(2) and slightly related with internal browning.

땅콩寄生 線? 防除에 關한 硏究

崔永然,金浩烈 COLLEGE OF AGRICULTURE KYUNGPOOK NATIONAL UNIVERSI 1983 慶北大農學誌 Vol.1 No.-

Nine species of plant parasitic nematodes, Aphelenchoides besseyi, Aphelenchus avenae, Criconemoides informis, Helicotylenchus dihystera, Meloidogyne arenaria, Meloidogyne hapla, Pratylenchus minyus and Tylenchus sp. Were found in peanut field in Korea. Chemicals used were; Telon C-17, Mocap 10G and Carbofuran 3G for control peanut parasitic nematodes. All chemicals reduced nematode populations but varied in effectiveness. Telon C-17 was especially effective against Meloidogyne hapla, the principal species on peanut and resulted in significant yield increased than the control. Plant height, number of branches and dry weight of peanut were increased over the nontreated control by chemical soil treatments.