Effects of Phenytoin and Diazepam on the Seizure Activity in the Cortical Dysplasia Animal Models

Kim, Si-Hyung,Choi, In-Sun,Cho, Jin-Hwa,Park, Eun-Ju,Jang, Il-Sung,Choi, Byung-Ju,Kim, Hyun-Jung,Kim, Young-Jin,Nam, Soon-Hyeun The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Dysplasia-associated seizure disorders are markedly resistant to pharmacological intervention. Relatively little research has been conducted studying the effects of antiepileptic drugs(AEDs) on seizure activity in a rat model of dysplasia. We have used rats exposed to methylazoxymethanol acetate(MAM) in utero, an animal model featuring nodular heterotopia, to investigate the effects of AEDs in the dysplastic brain. Pilocarpine was used to induce acute seizure in MAM-exposed and age-matched vehicle-injucted control animals. Field potential recordings were used to monitor amplitude and numbers population spikes, and paired pulse inhibition in response to stimulation of commissural pathway. Two commonly used AEDs were tested: diazepam 5, 2.5mg/kg;phenytoin 40, 60mg/kg. Diazepam(DZP) and phenytoin(PHT) reduced the amplitude of population spike in control and MAM-exposed rats. However, the amplitude of population spike was nearly eliminated in control rats as compared to the MAM-exposed rats. Pharmaco-resistance was tested by measuring seizure latencies in awake rats after pilocarpine administration(320mg/kg, i.p.) with and without pretreatment with AEDs. Pre-treatment with PHT 60 mg prolonged seizure latency in control rats, but not in MAM-exposed animals. The main findings of this study are that acute seizures initiated in MAM-exposed rats are relatively resistant to standard AEDs assessed in vivo. These data suggest that animal model with cortical dysplasia can be used to screen the effects of potential AEDs.

The combustion characteristics of vehicle upholsteries

Park,Hyung-Ju,Kang,Young-Goo,Kim,Hong 한국화재소방학회 1997 한국화재소방학회 학술대회 논문집 Vol.1997 No.-

Five parts of vehicle upholsteries were sampled to determine its combustion characteristics. Oxygen Indexer, Smoke chamber, Differential Scanning Calorimetry(DSC) are applied as the analysis apparatus. All LOI values of samples appear less than 21. The combustion phenomena of vehicle upholster primarily depends on properties of each layer material. The amount of smoke generated is the experiment reached the maximum value within 30-90sec after ignition. The experimental results of combustion characteristics and DSC of H/Line also indicated that the layer of foam was melt first and it caused the propagation of flame through the sample. The combustion characteristics of multi layer materials primarily depend on thermal characteristic of single layer material.

前腕部 經穴 取穴에서 骨度分寸法과 一夫法의 比較 硏究

박히준,채윤병,차웅석,박종배,이혜정,이향숙,인창식,고형균,김수영,최일환,김강식,문정배,배기태,유경환,육근영,정병주,손인철,임사비나 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2004 東西醫學硏究所 論文集 Vol.2004 No.-

Background : The cun measurement System, an essential and convenient method in locating acupoints, has been widely used in the practice of acupuncture. However, traditional cun measurement has been criticized for its lack of reliability. Objectives : The purposes of this study are to determine if one cun measured by the directional methods have a consistency with that of proportional methods and to investigate which factors are related with these differences, especially in forearm. Methods : The distance between the elbow crease and the wrist crease of forearm was compared to a reference value of one cun obtained by the directional method. In this method, one cun is one third of the distance between index finger and Small finger of a subject, measured at proximal interphalangeal joint. In addition, to investigate the factors influencing the differences between these two methods, we measured the height and body weight and calculated body mass index (BMI). Finally we analyzed the factors correlated with these lengths by linear regression test. Results : The results showed that one cun obtained by the directional methods were significantly different from one cun by the proportional methods in forearm. It was demonstrated that the length acquired with the directional method was more correlated with body weight and body mass index, while the length obtained by the proportional method was more correlated with the height.

초기 성공적인 심폐소생술후 혈청 Interleukin-6 농도의 발현양상

박규남,박승현,황두영,김형국,이환,최경호,이원재,정욱성,김학주,김세경 大韓應急醫學會 1998 대한응급의학회지 Vol.9 No.4

Objective : To investgate patterns of serial serum interleukin-6(IL-6) concentrations after initial successful cardiopulmonary resuscitation, and to access differences in serum IL-6 concentrations in patients with Cerebral Performance Category(CPC, 1-2) and CPC(3-5) at the 5th day after cardiacpulmonary resuscitation(CPR). Methods : We studied prospectively 12 patients with spontaneous circulation after cardiopulmonary resuscitation. Venous blood samples were taken 2, 4, 12, 24, 48 and 72 hours after restoration of spontaneous circulation to measure serum IL-6 levels by the enzyme-linked immunosorbent assay. Results : Serum IL-6 concentrations in the severe neurolgically disabled group(CPC, 3-5) were significantly increased at 2, 24 and 48 hours after restoration of spontaneous circulation compared with the less neurolgically disabled group. Also, serum IL-6 concentrations in the severe neurolgic disabled group showed dual peak at 4 and 24 hours. Conclusion : Our study demonstrates significant increase of serum IL-6 in patients with CPC(3-5) after successful cardiopulmonary resuscitation and the significant correlation between early increase of serum IL-6 and poor neurologic outcome. It suggests that early increase of serum IL-6 can be used as a prognostic marker after successful cardiopulmonary resuscitation.

Anti-idiotypic 항체가 Hybridoma 세포의 Anti-DNA 항체 합성을 조절하는 기전

박선,박정수,김형일,주민경,장영주,윤정구,김영태 아주대학교 의과학연구소 1996 아주의학 Vol.1 No.1

The regulatory mechanism of anti-idiotypic antibody(anti-id Ab) in the immune response is not fully understood. It has been reported that anti-id Ab suppresses Ab production of lymphocytes or hybridoma cells in vitro, but it does not affect the proliferation of cells or Ab secretion. We investigated whether or not polyclonal anti-id Ab regulates the production of anti-DNA Ab(lgG2a) in hybridoma cells. In addition, the effects of anti-id Ab on the proliferation of cells and on the transcription levels of lg genes were studied to elucidate the cellular and molecular mechanisms for regulation of anti-DNA Ab production in hybridoma cells. The treatment of hybridoma cells with anti-id Ab resulted in an increase in the number of cells producing anti-DNA Ab. However, there was no significant difference in the proliferation rate and mRNA levels of Ig genes between hybridoma cells treated with anti-id Ab, and those treated with normal rabbit serum. These results and our previous data suggest that the number of hybridoma cells producing anti-DNA Ab by anti-id Ab is not directly related with the rate of proliferation of the cells. Furthermore, it appears that the number of hybridoma cells producing IgG class anti-DNA Ab is increased by anti-id Ab, whereas the number of hybridoma cells producing IgM class anti-DNA Ab is down-regulated by anti-id Ab.

핀의 최적 설계를 위한 구조화 표본추출 적용 연구

박홍준,김정호,전형주 순천대학교 2000 순천대학교 자연과학논문집 Vol.19 No.1

The Taguchi method by orthogonal array, one of the structured sampling methods, has many advantages which are row cost and time saving for experiments and researches. But this method has applied limited area especially about mechanical problems. So, we experimented whether the structured sampling is useful to apply optimal design of mechanical elements. First, we made a mechanical problem to determine optimal parameters of a pin with a hole. The second, we calculated combination of optimal parameters by simulation. At the same time, we seeked optimal combination by structured sampling using orthogonal array and ANOVA(Analysis of Variance). The last, we compared these two results. The experimental result have shown that the structured sampling method seeked optimal parameters exactly. So, we see some possibility in this application.

본태성 혈소판 증다증 2예

주기산,임현성,김미자,양태영,허경,김완중,박유환,정춘해 朝鮮大學校 附設 醫學硏究所 1993 The Medical Journal of Chosun University Vol.18 No.1

Essential thrombocythemia is a chronic myeloproliferative disorder of unknown origin, charcterized by excessive number of morphologically and functionally abnormal platelet in peripheral blood and abnormal proliferation of megakaryocytes in bone marrow. Its main clinical manifestations are hemorrhages and thromboses. The aim of therapy is suppression of the excessive bone marrow activity, which can be achieved by radioactive phosphorus, alkylating agent, hydroxyurea and interferon. Two cases were diagnosed at our hospital which had laboratory and clinical features compatible with this disease. We have successfully treated patient with hydroxyurea and platelet antiaggregating agent.

마그네슘, 칼륨 및 기타의 금속이온이 Haloarcula sp. EH-1의 생육과 형태에 미치는 영향

박형숙,정명주 경성대학교 1997 論文集 Vol.18 No.1

Effect of magnesium, potassium, and some metal ions on the growth and morphology of Haloarcula sp. EH-1 were investigated. Maximum growth of the Haloarcula sp. EH-1 occured in the presence of 0.1 to 0.5 M magnesium ion and the production of carotenoid was directly proportional to the growth of this organism. Above 0.08 Mg?? the majority of cells were rod forms but below 0.03 Mg?? it became sphere forms. The growth of Haloarcula sp. EH-1 was stimulated by 10 ppm iron, 70 ppm calcium, and 40 ppm cupper ions, respectively.

치아 우식증의 조기 진단을 위한 QLF-D 개발

박형주,김종수,유승훈,신지선 大韓小兒齒科學會 2011 大韓小兒齒科學會誌 Vol.38 No.4

QLF-D system composed with DSLR(digital single-lens reflex) camera, and the images of natural enamel caries and artificial caries was developed from 2 days to 14 days captured by QLF-D system. The correlation between lesion depth of the polarized microscope and luminosity ratio of QLF-D image was analyzed and the results were summarized as follows: 1. The Pearson correlation value between the lesion depth of polarized microscope images and luminosity ratio of QLF-D images was 0.969(p<0.01). 2. From Regression analysis of lesion depth from polarized image by demineralized period, the equation was y = 8.67x - 1.16(p<0.05). 3. From Regression analysis of luminosity ratio from QLF-D image by demineralized period, the equation was y = 3.53x + 6.42(p<0.05). From the results, QLF-D system can detect the enamel caries at the very early stage and can monitor the progression of demineralization and remineralization. For the convenient use of QLF-D system in the laboratory, the image analysing software was needed to analyze of interest site of enamel caries lesion. 저자는 DSLR(digital single-lens reflex) 카메라를 이용한 QLF-D 시스템을 고안하여, 사람의 유구치에 자연 발생된 법 랑질 초기 우식증을 관찰하고, 유구치 법랑질 시편을 대상으로 2일부터 14일까지 인공 탈회시킨 후 탈회 기간에 따른 상관관 계를 편광 현미경 소견과 비교 분석한 결과 다음과 같은 결과를 얻었다. 1. 탈회 기간에 따른 편광 현미경 상의 병소 깊이와 QLF-D 영상의 광밀도 값 비율 간의 피어슨 상관 계수는 0.969였다 (p<0.01). 2. 편광현미경에서 측정된 병소 깊이의 탈회 기간에 대한 회귀분석 결과 y=8.67x-1.16의 회귀 방정식이 산출되었다 (p<0.05). 3. QLF-D 영상에서 측정된 광도값 비율의 탈회 기간에 대한 회귀분석 결과 y=3.53x+6.42의 회귀 방정식이 산출되었다 (p<0.05).

천일염으로부터 고호염균의 및 동정

빅형숙,정명주 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.6

시판 천일염으로부터 고호염성균을 분리하여 동정하였다. 분리균주는 그람음성, 통성혐기성으로 운동성을 나타내었다. 배양적 특성은 콜로니에 형태가 둥글고 볼록하였고, 지름은 약 2mm 정도이며, red-orange색을 나타내었다. Mg^2+ ion의 농도에 따라서 간상 또는 구형으로 다형태성을 나타내었고, 생육온도 범위는 35~45℃, 생육 pH 범위는 7.0~9.0이었다. 생육은 40℃에서 NaCl의 농도가 4.3~5.0 M에서 최대를 나타내었다. 생리적 특성으로 catalase, oxidase 양성이며, 전분을 분해하였고, bacitracin 감수성을 나타내었다. 다양한 당이용성를 나타내며, DNA의 G+C 함량은 62.7 mol%이었다. 이상의 결과로부터 분리균주는 Haloarcula 속으로 동정되어 Haloarcula sp. EH-1으로 명명하였다. An Extremely halophilic bacterium was isolated from solar salts. The isolated strain was Gram-negative, facultatively anaerobic, and motile bacterium. The colony was circular, smooth, and red-orange color. The strain showed pleomorphism depending on magnesium ion concentrations. The range of temperature and pH for growth of the isolate were 35~45℃ and 7.0~9.0. Nacl concentration for growth of it was 4.3~5.0 M. The isolate was catalase and oxidase positive, and sensitive to bacitracin. It showed starch hydrolyzing and acid forming characteristics. DNA G+C content was 62.7 mol%. The morphological, physiological and biochemical characteristics of the isolate resembled those of the Haloarcula vallisumortis, therefore it was identified as Haloarcula sp. EH-1.