Molecular and functional characterization of the adiponectin (AdipoQ) gene in goat skeletal muscle satellite cells

Wang, Linjie,Xue, Ke,Wang, Yan,Niu, Lili,Li, Li,Zhong, Tao,Guo, Jiazhong,Feng, Jing,Song, Tianzeng,Zhang, Hongping Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.8

Objective: It is commonly accepted that adiponectin binds to its two receptors to regulate fatty acid metabolism in adipocytes. To better understand their functions in the regulation of intramuscular adipogenesis in goats, we cloned the three genes (adiponectin [AdipoQ], adiponectin receptor 1 [AdipoR1], and AdipoR2) encoding these proteins and detected their mRNA distribution in different tissues. We also determined the role of AdipoQ in the adipogenic differentiation of goat skeletal muscle satellite cells (SMSCs). Methods: SMSCs were isolated using 1 mg/mL Pronase E from the longissimus dorsi muscles of 3-day-old female Nanjiang brown goats. Adipogenic differentiation was induced in satellite cells by transferring the cells to Dulbecco's modified Eagle's medium supplemented with an isobutylmethylxanthine, dexamethasone and insulin cocktail. The pEGFP-N1-AD plasmid was transfected into SMSCs using Lipofectamine 2000. Expression of adiponectin in tissues and SMSCs was detected by quantitative polymerase chain reaction and immunocytochemical staining. Results: The three genes were predominantly expressed in adipose and skeletal muscle tissues. According to fluorescence and immunocytochemical analyses, adiponectin protein expression was only observed in the cytoplasm, suggesting that adiponectin is localized to the cytoplasm of goat SMSCs. In SMSCs overexpressing the AdipoQ gene, adiponectin promoted SMSC differentiation into adipocytes and significantly (p<0.05) up-regulated expression of AdipoR2, acetyl-CoA carboxylase, fatty-acid synthase, and sterol regulatory element-binding protein-1, though expression of CCAAT/enhancer-binding $protein-{\alpha}$, peroxisome proliferator-activated receptor ${\gamma}$, and AdipoR1 did not change significantly. Conclusion: Adiponectin induced SMSC differentiation into adipocytes, indicating that adiponectin may promote intramuscular adipogenesis in goat SMSC.

Damage assessment in periodic structures from measured natural frequencies by a sensitivity and transfer matrix-based method

Zhu, Hongping,Li, Lin,Wang, Dansheng Techno-Press 2003 Structural Engineering and Mechanics, An Int'l Jou Vol.16 No.1

This paper presents a damage assessment procedure applied to periodic spring mass systems using an eigenvalue sensitivity-based method. The damage is directly related to the stiffness reduction of the damage element. The natural frequencies of periodic structures with one single disorder are found by adopting the transfer matrix approach, consequently, the first order approximation of the natural frequencies with respect to the disordered stiffness in different elements is used to form the sensitivity matrix. The analysis shows that the sensitivity of natural frequencies to damage in different locations depends only on the mode number and the location of damage. The stiffness changes due to damage can be identified by solving a set of underdetermined equations based on the sensitivity matrix. The issues associated with many possible damage locations in large structural systems are addressed, and a means of improving the computational efficiency of damage detection while maintaining the accuracy for large periodic structures with limited available measured natural frequencies, is also introduced in this paper. The incomplete measurements and the effect of random error in terms of measurement noise in the natural frequencies are considered. Numerical results of a periodic spring-mass system of 20 degrees of freedom illustrate that the proposed method is simple and robust in locating single or multiple damages in a large periodic structure with a high computational efficiency.

Study of Hybrid DNA Physical Mapping Based on Approximation Algorithm with Errors

Zhenglong Liu,Yanmei Yang,Yujun Luo,Hongping Wang 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.2

A human chromosome is a DNA molecule with approximately 108 base pairs. The techniques developed to date for sequencing are restricted to pieces of DNA with up to tens of thousands of base pairs. This means that when a piece is sequenced, only an extremely small part of a chromosome can be seen. Molecular biologists use special techniques to deal with DNA molecules comparable in size to a chromosome. These techniques enable them to create maps of an entire chromosome or of significant fractions of chromosomes. Computational techniques were studied that could potentially aid biologists in the map-generation process. An algorithm that solves the consecutive 1s problem was studied. Such a problem is a good model of hybridization mapping when there are no errors and when probes are unique. If errors are present, another approach is needed, and the approximation algorithm is a prospective problem solver for hybridization physical mapping of DNA with errors.

Glucocorticoid-induced expansion of classical monocytes contributes to bone loss

Liu Pei,Gao Youshui,Luo Pengbo,Yu Hongping,Guo Shang,Liu Fuyun,Gao Junjie,Xu Jianzhong,Wang Shengdian,Zhang Changqing 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Classical monocytes are commonly involved in the innate inflammatory response and are the progenitors of osteoclasts. Excess endogenous glucocorticoids (GCs) can increase the levels of classical monocytes in blood and bone marrow. The role of this cell population in high-dose exogenous GC-induced osteoporosis (GIOP) remains to be elucidated. In this study, GIOP was established in rats and mice by daily methylprednisolone injection, and monocyte subsets were analyzed by flow cytometry. We demonstrated that classical monocytes accumulate in bone marrow during GIOP. Similarly, the monocyte proportion among bone marrow nucleated cells was also increased in patients with steroid treatment history. We sorted classical monocytes and analyzed their transcriptional profile in response to GCs by RNA sequencing. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis showed that classical monocytes isolated from GC-treated rats exhibited osteoclast differentiation potential. Deletion of classical monocytes by clodronate liposome treatment prevented GIOP via inhibition of osteoclastogenesis and restoration of CD31HiendomucinHi vessels. Regarding the molecular mechanism, classical monocytes express high levels of glucocorticoid receptors. In vitro treatment with GCs increased both the percentage and absolute number of monocytes and promoted their proliferation. In summary, classical monocytes mediated GC-induced bone loss and are a potential target for therapeutic intervention in GIOP treatment.

Efficiently targeted therapy of glioblastoma xenograft via multifunctional biomimetic nanodrugs

Zhipeng Yao,Xiaochun Jiang,Hong Yao,Yafeng Wu,Fan Zhang,Cheng Wang,Chenxue Qi,Chenhui Zhao,Zeyu Wu,Min Qi,Jia Zhang,Xiaoxiang Cao,Zhichun Wang,Fei Wu,Chengyun Yao,Songqin Liu,Shizhang Ling,Hongping Xi 한국생체재료학회 2022 생체재료학회지 Vol.26 No.4

Background: Glioblastoma multiforme (GBM) is a fatal malignant primary brain tumor in adults. The therapeutic efficacy of chemotherapeutic drugs is limited due to the blood-brain barrier (BBB), poor drug targeting, and short biological half-lives. Multifunctional biomimetic nanodrugs have great potential to overcome these limitations of chemotherapeutic drugs. Methods: We synthesized and characterized a biomimetic nanodrug CMS/PEG-DOX-M. The CMS/PEG-DOX-M effectively and rapidly released DOX in U87 MG cells. Cell proliferation and apoptosis assays were examined by the MTT and TUNEL assays. The penetration of nanodrugs through the BBB and anti-tumor efficacy were investigated in the orthotopic glioblastoma xenograft models. Results: We showed that CMS/PEG-DOX-M inhibited cell proliferation of U87 MG cells and effectively induced cell apoptosis of U87 MG cells. Intracranial antitumor experiments showed that free DOX hardly penetrated the BBB, but CMS/PEG-DOX-M effectively reached the orthotopic ntracranial tumor through the BBB and significantly inhibited tumor growth. Immunofluorescence staining of orthotopic tumor tissue sections confirmed that nanodrugs promoted apoptosis of tumor cells. This study developed a multimodal nanodrug treatment system with the enhanced abilities of tumor-targeting, BBB penetration, and cancer-specific accumulation of chemotherapeutic drugs by combining chemotherapy and photothermal therapy. It can be used as a flexible and effective GBM treatment system and it may also be used for the treatment of other central nervous systems (CNS) tumors and extracranial tumors.

Open Access : Expression patterns of TRα and CRABPII genes in Chinese cashmere goat skin during prenatal development

( Tao Zhong ),( Wei Zhao ),( Zhongqiang Zhou ),( Li Li ),( Linjie Wang ),( Hua Li ),( Hongping Zhang ) 한국동물자원과학회(구 한국축산학회) 2015 한국축산학회지 Vol.57 No.28

Background: The physiologic characteristics of the cashmere trait and many of the differentially expressed genes relevant to hair cycling have been extensively studied, whereas genes involved in the prenatal development of hair follicles have been poorly investigated in cashmere goats. The aim of this study, therefore, was to quantify the time-course changes in the expressions of TRα and CRABPII genes in the fetal skin of Chinese cashmere goats at the multiple embryonic days (E70, E75, E80, E90, E100, E120 and E130) using real-time quantitative PCR (RT-qPCR). Results: RT-qPCR showed that TRα was expressed at E70 with relatively high level and then slightly decreased (E75, E80, and E90). The highest expression of TRα mRNA was revealed at E130 (P > 0.05). The expression pattern of CRABPII mRNA showed an ``up-down-up`` trend, which revealed a significantly highest expression at E75 (P < 0.05) and was down-regulated during E80 to E120 (P < 0.05) and mildly increased at E130, subsequently. Conclusion: This study demonstrated that TRα and CRABPII genes expressed in different levels during prenatal development of cashmere. The present study will be helpful to provide the comprehensive understanding of TRα and CRABPII genes expressions during cashmere formation and lay the ground for further studies on their roles in regulation of cashmere growth in goats.

Expression patterns of TRα and CRABPII genes in Chinese cashmere goat skin during prenatal development

Zhong, Tao,Zhao, Wei,Zhou, Zhongqiang,Li, Li,Wang, Linjie,Li, Hua,Zhang, Hongping Korean Society of Animal Science and Technology 2015 한국축산학회지 Vol.57 No.8

Background: The physiologic characteristics of the cashmere trait and many of the differentially expressed genes relevant to hair cycling have been extensively studied, whereas genes involved in the prenatal development of hair follicles have been poorly investigated in cashmere goats. The aim of this study, therefore, was to quantify the time-course changes in the expressions of $TR{\alpha}$ and CRABPII genes in the fetal skin of Chinese cashmere goats at the multiple embryonic days (E70, E75, E80, E90, E100, E120 and E130) using real-time quantitative PCR (RT-qPCR). Results: RT-qPCR showed that $TR{\alpha}$ was expressed at E70 with relatively high level and then slightly decreased (E75, E80, and E90). The highest expression of $TR{\alpha}$ mRNA was revealed at E130 (P > 0.05). The expression pattern of CRABPII mRNA showed an 'up-down-up' trend, which revealed a significantly highest expression at E75 (P < 0.05) and was down-regulated during E80 to E120 (P < 0.05) and mildly increased at E130, subsequently. Conclusion: This study demonstrated that $TR{\alpha}$ and CRABPII genes expressed in different levels during prenatal development of cashmere. The present study will be helpful to provide the comprehensive understanding of $TR{\alpha}$ and CRABPII genes expressions during cashmere formation and lay the ground for further studies on their roles in regulation of cashmere growth in goats.

Host Langerin (CD207) is a receptor for <i>Yersinia pestis</i> phagocytosis and promotes dissemination

Yang, Kun,Park, Chae G,Cheong, Cheolho,Bulgheresi, Silvia,Zhang, Shusheng,Zhang, Pei,He, Yingxia,Jiang, Lingyu,Huang, Hongping,Ding, Honghui,Wu, Yiping,Wang, Shaogang,Zhang, Lin,Li, Anyi,Xia, Lianxu,B Nature Publishing Group 2015 Immunology and Cell Biology Vol. No.

<P><I>Yersinia pestis</I> is a Gram‐negative bacterium that causes plague. After <I>Y. pestis</I> overcomes the skin barrier, it encounters antigen‐presenting cells (APCs), such as Langerhans and dendritic cells. They transport the bacteria from the skin to the lymph nodes. However, the molecular mechanisms involved in bacterial transmission are unclear. Langerhans cells (LCs) express Langerin (CD207), a calcium‐dependent (C‐type) lectin. Furthermore, <I>Y. pestis</I> possesses exposed core oligosaccharides. In this study, we show that <I>Y. pestis</I> invades LCs and Langerin‐expressing transfectants. However, when the bacterial core oligosaccharides are shielded or truncated, <I>Y. pestis</I> propensity to invade Langerhans and Langerin‐expressing cells decreases. Moreover, the interaction of <I>Y. pestis</I> with Langerin‐expressing transfectants is inhibited by purified Langerin, a DC‐SIGN (DC‐specific intercellular adhesion molecule 3 grabbing nonintegrin)‐like molecule, an anti‐CD207 antibody, purified core oligosaccharides and several oligosaccharides. Furthermore, covering core oligosaccharides reduces the mortality associated with murine infection by adversely affecting the transmission of <I>Y. pestis</I> to lymph nodes. These results demonstrate that direct interaction of core oligosaccharides with Langerin facilitates the invasion of LCs by <I>Y. pestis</I>. Therefore, Langerin‐mediated binding of <I>Y. pestis</I> to APCs may promote its dissemination and infection.</P>