신규 방사성 항암제 DW-166HC 의 소핵시험

문은이(Eun Yi Moon),이진(Jin Lee),이원용(Won Yong Lee),최청하(Chung Ha Choi),이덕근(Dug Keun Lee),유제만(Jei Man Ryu),정용호(Yong Ho Chung),윤성준(Sung June Yoon),박경배(Kyung Bae Park) 한국응용약물학회 1997 Biomolecules & Therapeutics(구 응용약물학회지) Vol.5 No.3

DW-166HC (^(166)Holmium (^(166)Ho)-Chitosan complex) is a new radiopharmaceutic anticancer agent with a broad anti-tumorigenic spectrum, especially against human hepatic cancer. DW-166HC was evaluated for the appearance of micronucleus in polychromatic erythrocytes (PCEs) of mouse bone marrow cells after subcutaneous arid intravenous single administration. Bone marrow cells were prepared at 24 hr and 48 hr after DW-166HC-I (^(165)Ho-Chitosan complex : cold compound) administration and at 24 hr, 72 hr and 2 weeks after DW-166HC (^(166)Ho-Chitosan complex : hot compound) administration. The results showed there was no statistically significant increase of the numbers of PCEs with micronucleus in all DW-166HC-I administered groups compared with a negative control group but there was statistically significant increase of the numbers of PCEs with micronucleus at 24 hr arid 72 hr in all DW-166HC administered groups, which was recovered after 2 weeks from the drug administration. The results also showed the ratio of normochromatic erythrocytes (NCEs) to PCEs of all DW-166HC-I administered groups was not significantly different from that of a negative controi group but there was significant difference of this ratio at 24hr and 72 hr in all DW-166HC administered groups compared with that of negative group, which was also recovered after two weeks from the drug administration. These results suggested that DW-166HC-I may not cause any chromosomal damage but DW-166HC has in vivo mutagenic potential because of its radioactivity.

가소제에 따른 탈지강 단백질 필름의 물리적 특성

배동호,김경미 建國大學校附設農業資源開發硏究所 2002 農資源開發論集 Vol.24 No.-

Biodegradable firms were prepared from rice bran protein with 1%, 1.5%, 2% and 2.5% glycerol and 1%, 1.5%, 2% and 2.5% sorbitol. The effects of glycerol and sorbitol contents on tensile strength, % elongation and water vapour permeability of the films were studied. Films prepared with sorbitol had higher tensile strengh, lower % elongation and lower water vapour permeability than the films prepared with glycerol.

IgG GM₁ 항체와 Campylobacter jejuni 항체와 관련된 급성 운동축삭형 신경병증

홍경훈,김승현,이영배,이영주,김희태,김주한,김명호 대한신경과학회 1997 대한신경과학회지 Vol.15 No.5

아황산가스가 흰쥐 허파조직내 Laminin 활서에 미치는 영향에 대한 면역조직학적 연구

배성만,정호삼,서윤경,백두진,김원규,윤지희 한양대학교 의과대학 2000 한양의대 학술지 Vol.20 No.2

Sulfur dioxide (SO_2), a kind of air pollutant, causes harmful damage to human body. In particular, inhalation of sulfoxide dioxide has been demonstrated to result in the injury to the upper respiratory duct. However, mechanisms by which SO_2 affects these tissues remain to be clarified. In the present study, to investigate the mechanism of SO_2 effects, the influence of SO_2 exposure was examined in terms of the injury of lung and the expression pattern of laminin in the basal lamina. The basal lamina is an important tissue for the regulation of internal respiration by composing of air-blood barrier. Sprague-Dawley rats repetitively exposed to a mixture of O_2 gas and SO_2 gas (250 ppm) for 30 minutes a day were sacrificed to observe the distribution of laminin in the alveolar septum as well as the morphological alteration of alveoli using immunohistological methods. The resutls we observed were as follows: 1. Alveoli from SO_2-exposed rats (for 5 days) were strongly stained with anti-laminin antibody, suggesting laminin expressed at the high level, at week 1 to 3 upon exposure to SO_2 2. At week 1 to 3 after exposure of rats to SO_2, alveolar septa were collapsed, leading to the reduction of alveolar volume along with morphological changes to irregular shapes. 3. At week 4 to 6 after exposure of rats to SO_2, alveoli were weakly stained with anti-laminin antibody, suggesting laminin expression was decreased during this period. 4. Rats sacrificed at week 7 upon exposure to SO_2 exhibited the expansion of new alveoli and the expression of laminin was partially recovered up to the intermediate level. Taken together, these results demonstrate that the expression of laminin was enhanced in the early phase, followed by downregulation in the late phase. Moreover, lung injury and resolution were correlated with the level of laminin. Thus, these results suggest that SO_2 suppresses the expression of laminin, which may be associated with the neo-generation of lung tissue.

다량의 균일한 액적 제조를 위한 분리막 기반 미세유체 소자 시스템

장문배, 유진오, 염경호 충북대학교 산업과학기술연구소 2013 산업과학기술연구 논문집 Vol.27 No.2

In this study, we designed the microfluidic device system based on the separation membrane for generating monodisperse emulsion droplets with diameters ranging from hundreds of nanometers to several micrometers. To generate fine droplets, T-junction and flow-focusing geometry are integrated into the microfluidic channel. Also put the membrane into the microfluidic device to make droplets effectively. PDMS devices can be easily, quickly, and inexpensively fabricated with superb resolution. However, PDMS devices are swelled other organic solvent. So we could produce droplets using fluorocarbon oil. The droplet size was controlled by adjusting the flow ratio between continuous phase flow and dispersed phase flow and also membrane pore size

열공형과 비열공형 피질하 혈관성 치매에서 위험인자의 차이에 관한 비교 연구

배희준,정지향,유경호,나덕렬,김상윤,최경규,양동원,손의주,이상도,김재우,박경원,김응규,이재홍,박미영,한일우,함동석,최문성,하충건,최성혜,이애영,이병철,한설희 대한치매학회 2003 Dementia and Neurocognitive Disorders Vol.2 No.2

Backgrounds and Objectives: Vascular dementia is a group of dementing disoders arising from various stroke syndrome. Among these. subcortical ischemic vascular dementia (SIVD) is regarded as a relatively distinct clinical entity. However, MRI patterns of SIVD are not homogenous. In some patients, lacunes are dominant, and in others, subcortical white matter changes are. This study was designed to compare risk factor profiles between SIVD with and without multiple lacunes. Methods: We divided 47 subjects (22 males, mean age. 68 years) recruited from VADAPET (Multicenter Trial For Evaluation Of The Changes In the PET Images Of Subcortical Vascular Dementia Patient) study into two groups one with more than 5 lacunes in deep gray matter (lacune group) and the other with 5 or less(non-lacune group) Clinical characteristics and laboratory findings of two groups were compared. Results: Nineteen of 47 patients (40%) belonged to the lacune group. The lacune and non-lacune groups d d not differ in the following variables: age, hypertension, diabetes mellitus, hyperlipidemia heart disease, history of stroke or TIA, history of trauma or major surgery, family history of hypertension stroke, or dementia, age at diagnosis of dementia, body mass index, white blood cell count, ESR, CRP, fibrinogen, hemoglobin A1C, total cholesterol. LDL cholesterol creatinine, proteinuria, glucosuria, and microhematuria. However, male sex, smoking alcohol. hemoglobin, and HDL cholesterol were possibly associated more with lacune group SIVD than with non-lacune group (p<0 1) Multivariate analyses revealed that smoking, hemoglobin, and HDL cholesterol were independent predictors of SIVD with multiple lacunes Conclusion: Our study suggests that SIVD with multiple lacunes may be significantly different in smoking habits hemoglobin, and HDL cholesterol from SIVD without multiple lacunes.

골격성 Ⅱ급 부정교합자의 악교정 수술 후 설골의 위치와 상기도 변화에 관한 연구

배진성,김경호,박형식,허종기,박광호 大韓顎顔面成形再建外科學會 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.6

OSAS(obstructive sleep apnea syndrome) is becoming more and more of importance nowadays. Therefore the study concerning OSAS is very important. Most of the studies which have been reported were comparison of upper airway size and change of skeletal class Ⅰ group and skeletal class Ⅲ group respectively. But, most of the showing OSAS have pharyngeal narrowing combined with more or less distinct maxillary and mandibular deficiency. Therefore the aim of this study is to see posterior airway space and hyoid bone position in patients affected by class Ⅱ malocclusion and treated with orthognathic surgery. we measured the lines between selected upper air way landmarks on lateral cephalometric x-ray films of skeletal class Ⅱ 30 persons who had not been operated yet, were within 2 weeks after operation, were 6 months after operation. And we compared them respectively and analyzed them with pairing t-test, pearson correlation coefficient and multiple regression analysis. The results were as follows. First of all, skeletal class Ⅱ group was increased in all the level of PAS, especially PAS(MK) and PAS related to hyoid bone(H-phW, H-PhW(Me-H)) was significantly decreased within 6 months after operation but that was larger than preoperation. And then the PAS was increased after mandibular advancement surgery. While there was no significant correlation between PAS and surgical methods, there was statistical significance between PAS and a number of surgical movement. At last, the hyoid bone was advanced within 2 weeks after operation but relapsed within the follow up period. In the end of the study, it was turned out that PAS was always wider without distinction of the region after the operation and that there was significant correlation between the moving volume of hard tissues and the changing quantity of PAS( ; how much PAS was changed according to the moving degree of hard tissues). After this, to be based on the study, I consider that OSAS demands further study and that it is necessary to do more practical applications to the patients.

원발성 후복막 점액낭샘암종 1예

지준호,이화정,박승찬,박정철,최은정,서혜진,이원식,이정림,배병조,손경락,이경희 영남대학교 의과대학 2008 Yeungnam University Journal of Medicine Vol.25 No.2

Primary retroperitoneal mucinous cystadenocarcinoma is a very rare malignancy, and little is known concerning its Pathogenesis, optimal treatment, and prognosis. A 29-year-o1d pregnant woman (21 weeks) Presented with abdominal discomfort CA 19-9, CA 125, and CEA were normal Abdominal CT scanning revealed a 19x15x13 cm retropentoneal tumor Exploratory laparotomy and tumor excision were performed. Mucinous retropentoneal implants were removed as completely as possible Histologically, the tumor showed focal areas of capsular invasion, but free resection margins The uterus and both ovaries were normal in appearance No adjuvant therapy was pursued. Six months later, Peritoneal and bilateral ovarian metastases were discovered. Hence, we report the details of this case of primary retroperitoneal mucinous Cystadeno-carcinoma and present a review of the literature.

Co/Mn/Br계 촉매상에서 p-자일렌 액상산화반응의 공정변수에 관한 연구

손용배,박경린,류태공,김정희,오인석,김영호,양현수 忠南大學校 産業技術硏究所 2001 산업기술연구논문집 Vol.16 No.2

Liquid phase oxidation of p-xylene using molecular oxygen has been carried out on the Co/Mn/Br-based catalyst system in acetic acid as a solvent. Process parameters(total pressure, partial pressure of oxygen and temperature) and kinetics of the reaction were studied as a basic study in the viewpoint for the development of new catalyst system and process. The total pressure above 20atm and the partial pressure of oxygen above 5atm were required to keep the reactant in the liquid phase and to overcome the mass transfer limitation of oxygen. The oxidation rate conversion were increased with increasing reaction temperature to the 100∼190℃ range. For a given initial concentration of p-xylene, the oxidation rate and the apparent activation energy were found to be of first-order and 4.24 ㎉/㏖, respectively. The effect of the third components added to the Co/Mn/Br catalyst was also studied. It was found that Ni is only a good additive that can promote the catalyst performance.

치아이동에 의한 백서 삼차신경감각핵군내 c-Fos의 발현

민경호,박효상,배용철,성재현 대한치과교정학회 1998 대한치과교정학회지 Vol.28 No.3

이 연구의 목적은 말초조직에 유해 자극을 가하였을 때 중추 신경계내 이차 신경 세포체내에 발현되어 Neuronal marker로 사용되고 있는 c-fos를 사용한 면역 조직화학법으로 치아이동시 동반되는 동통의 투사경로의 이해에 도움을 주고자하는 것이다. 생후 9 주령의 210gm내외의 Sprague-Dawley계 웅성 백서 21마리를 교정력을 가하지 않고 마취만을 시행한 정상대조군과 교정력 적용 시간 경과에 따라 1시간, 3시간, 6시간, 12시간, 1일, 3일군으로 나누어 각 해당 시간동안 상악 우측 제1대구치와 상악 우측 측절사이에 Ni-Ti coil spring을 결찰하여 30gm내외의 지속적인 교정력을 가한 후 희생시켰다. 희생시킨 백서의 뇌간을 적출하여 토끼의 항체를 이용하여 면역화학 염색을 시행하였다. 삼차신경 감각핵군내 부위에 따른 c-fos 면역 반응 세포를 측정하여 교정력 적용 시간 경과에 따른 변화를 관찰하였다. · c-fos면역 반응 세포의 배측에서의 분포는 자극측 중위핵과 미측핵의 이행부위에서 시작하여 제1경추 척수 후각에까지 이어졌는데 가장 많은 분포를 보인 곳은 미측핵의 문측 부위였다. 그리고 주로 I층 과 II층에서 관찰되었다. · 복측에서의 c-fos 면역 반응 세포의 분포는 자극측 중위핵의 미측 부위에서 시작하여 미측핵의 중간부위에 까지 이어졌다. · 교정력 적용 3,6시간군에서 c-fos면역 반응 세포가 가장 많이 관찰 되었으며 12시간군에서 감소되기 시작하여 1일, 3일군에서는 현저히 감소 하였다. 위로 미루어 볼 때 지속적인 교정력에 의한 동통은 중위핵과 미측핵의 이행부위, 미측핵, 제1경추 척수후각에서 매개되는 것으로 생각된다. The c-fos is known as neuronal marker of second neurons which is activated by noxious peripheral stimulation. To investigate the changes of c-fos expression in the trigeminal nucleus complex during tooth movement, immunohistochemical study was performed. Experimental rats(9 weeks old, 210 gm, 21 rats) were divided into seven groups(normal, 1 hour group, 3 hour group, 6 hour group, 12 hour group, 1 day group, 3 day group). Rats in the normal group were anesthesized without orthodontic force. Rats in the experimental groups were applied orthodontic force (approximately 30 gm) to upper right maxillary molar. Frozen sections of brain stem were immunostained using rabbit antisera. The change of c-fos expression were observed with respect to rostrocaudal distribution, laminar organization, and duration of orthodontic force application. The study results were as follows. · The c-fos nuclei in the dorsal part were observed from ipsilateral transition zone of subnucleus interpolaris and subnucleus caudalis to C1 cervical dorsal horn rostrocaudally. The maixmal peak point was the rostral part of subnucleus caudalis. The greatest proportion of c-fos cells were located within lamina and I and II. · The c-fos nuclei in the dorsal part were observed from the most caudal part of subnuleus interpolaris to the middle part of the subnucleus caudalis. · The number of c-fos immunoreactive dot increased at 1 hour group, reached its maximum at the 3 and 6 hour groups, and showed a decreasing trend after 12 hours. These resutls imply that nociceptive stimulation caused by continuous orthodontic force might be modulated by transition zone of subnucleus interpolaris and subnucleus caudalis, subnucleus caudalis, C1 spinal dorsal horn.