폐리튬 이차전지 황산침출용액에서 Cyanex 301에 의한 구리(II)의 선택적 추출

이세아,이만승 대한금속·재료학회 2019 대한금속·재료학회지 Vol.57 No.9

Spent lithium ion batteries contain cobalt, copper, lithium, manganese and nickel. Recovery of these metals with high purity from spent lithium ion batteries is of importance. A sulfuric acid solution is generally employed for the treatment of spent lithium ion batteries, but it is difficult to separate these metal ions from the leaching solution because the divalent metal ions have similar extraction behaviors. Once Cu(II) is separated from the sulfuric acid leaching solution, the traditional solvent extraction process for the separation of Co(II) and Ni(II) can be applied to the raffinate. Therefore, selective extraction of Cu(II) was investigated in this work using Cyanex 301. The effect of the concentration of sulfuric acid and Cyanex 301 was investigated. Within the sulfuric acid concentration range (pH 5-2.0 M) employed in this work, Cu(II) was completely extracted by Cyanex 301, while the extraction percentage of other metal ions decreased to negligible when the sulfuric acid concentration increased to 2.0 M. The addition of TBP to Cyanex 301 depressed the extraction of other metal ions except Cu(II) at an initial pH of 3, facilitating the selective extraction of Cu(II). The McCabe-Thiele extraction diagram indicates that most of the Cu(II) was extracted by two count-current extraction at an A/O ratio of 2. The Cu(II) loaded in Cyanex 301 was completely stripped by using 5% aqua regia.

Cytotoxicity of natural killer cells co-cultured with γ-irradiatedor mitomycin C-treated feeder cells

이세아,구나연,이시우,이지현,이윤희,현방훈 한국예방수의학회 2020 예방수의학회지 Vol.44 No.2

Natural killer (NK) cells are innate lymphocytes that aid in the protection of the host from infectious diseases and cancer. Regulation of cytotoxicity in NK cells is mediated by inhibitory receptors that bind major histocompatibility complex class I (MHC-I) molecules on target cells. Human myelogenous leukemia K562 cells are readily attacked by NK cells, because K562 cells expressed very low levels of MHC-I molecules for inhibitory NK cell receptors. In this study, we compared the ability of γ-irradiated- or mitomycin C (MMC)-treated K562 feeder cells to support expansion and activation of canine NK cells. We isolated CD5 negative cells from canine peripheral blood mononuclear cells by immunomagnetic separation and co-cultured with γ-irradiated (100 Gy)- or MMC (20 μg/mL)-treated K562 cells in the presence of interleukin (IL)-2, IL-15 and IL-21 for 21 days. As a result, number of CD5 negative cells, co-cultured with γ-irradiated- or MMC-treated K562 cells (56.72 ± 13.77 fold or 32.99 ± 10.83 fold), was increased than those of CD5 negetive cells (2.99 ± 1.42 fold). Also, we found that gene expression markers of activated NK cells such as NKp30, NKp44, NKp46, Ly49, NKG2D, CD244, perforin, and granzyme B and production of interferon gamma were similarly upregulated in NK cells co-cultured with γ-irradiated- or MMC-treated K562 cells, respectively. Next, we observed that cytotoxicity of NK cells co-cultured with γ-irradiated K562 cells was more sensitively reacted to canine mammary carcinoma cells than those of MMC-treated K562 cells. These results revealed that γ-irradiated K562 cells are more efficient feeder cells than MMC-treated K562 cells for enhancing NK cells expansion and activation.

급성 저주파 감각신경성 난청에서 스테로이드 용량에 따른 단기적 치료 효과 비교

이세아,이승재,정도영,김보경,이치규,최성준,이병돈,황규린,이종대 대한이비인후과학회 2018 대한이비인후과학회지 두경부외과학 Vol.61 No.4

Background and Objectives Acute low-tone sensorineural hearing loss refers to a condi-tion wherein thresholds increase only at low frequencies in pure-tone audiometry. Systemic steroid treatment is commonly used for acute low-tone sensorineural hearing loss without ver-tigo, but there has been no established treatment in most clinical researches. The purpose of this study is to compare and analyze the short-term effects of high- and half-dose oral steroid therapy on patients suffering from acute low-tone sensorineural hearing loss without vertigo. Subjects and Method Forty-six patients diagnosed with acute low-tone sensorineural hear-ing loss were divided into two groups and treated with high-dose (24 patients) and half-dose (22 patients) steroids, respectively. A retrospective analysis was conducted on their medical records. Results There were no significant differences between the two groups in any of the follow-ing: age, sex ratio, duration of symptoms before treatment, pure-tone hearing thresholds, low frequency hearing thresholds and speech intelligibility. However, both groups showed signifi-cant differences in hearing thresholds before and after treatment. In addition, no significant difference was noted in the recovery and recurrence rate between the two groups.Conclusion According to the results of this study, there is no difference in treatment effect between the high- and half-dose groups. Therefore, half-dose steroids may be used as a treat-ment option for acute low tone sensorineural hearing loss.

현훈을 동반한 돌발성감각신경성난청 환자에서의 전정기능과 예후

이세아,김효준,김보경,이종대 대한평형의학회 2018 Research in Vestibular Science Vol.17 No.3

Objectives: Vertigo combined with sudden sensorineural hearing loss (SSNHL) is known as a poor prognostic factor. We investigated clinical findings and vestibular function tests in patients of SSNHL with vertigo to find the prognostic factors.Methods: We retrospectively evaluated data on the patients diagnosed with SSNHL with vertigo at Bucheon Soonchunhyang University Hospital from March 2009 to February 2018. We reviewed medical records and the results of vestibular function tests and audiometry.Results: Of the 68 patients, 30 (44.1%) showed profound hearing loss and 53 (77.9%) showed poor recovery. Age and the degree of initial hearing loss showed negative prognostic factor in hearing recovery. Abnormal results of cervical vestibular evoked myogenic potentials (cVEMP) also showed significantly differences between good and poor recovery groups.Conclusions: In this study, most of the patients of SSNHL with vertigo showed poor recovery. Age, degree of initial hearing loss, and the abnormal result of the cVEMP have a negative effect on the prognosis of hearing recovery.

이관질환의 병태생리 및 분류

이세아,이치규 대한이비인후과학회 부산,울산,경남 지부회 2022 임상이비인후과 Vol.33 No.4

The Eustachian tube is the canal that connects the tympanic cavity with the nasopharynx, whose three main functions are ventilation, protection, and clearance. Eustachian tube dysfunction is directly related to the pathophysiology of the middle ear, as is the case with acute otitis media and otitis media with effusion. In the current review, we aim to provide background information on the anatomy, physiology, and pathophysiology of the eustachian tube.

Microscopic versus Endoscopic Inlay Butterfly Cartilage Tympanoplasty

이세아,강현택,이윤지,김보경,이종대 대한청각학회 2019 Journal of Audiology & Otology Vol.23 No.3

Background and Objectives: Inlay butterfly cartilage tympanoplasty makes the graft easy, and reduces operating time. The present study aimed to investigate the outcomes of microscopic versus endoscopic inlay butterfly cartilage tympanoplasty. Subjects and Methods: In this retrospective study, the outcomes of 63 patients who underwent inlay butterfly cartilage tympanoplasty with small to medium chronic tympanic membrane perforation were evaluated. Twenty-four patients underwent conventional microscopic tympanoplasty and 39 underwent endoscopic tympanoplasty. The outcomes were analyzed in terms of the hearing gain and graft success rate. Results: The surgical success rate was 95.8% in the patients who underwent conventional microscopic tympanoplasty and 92.3% in those who underwent endoscopic tympanoplasty. In both groups of patients, the postoperative air-bone gap (ABG) was significantly lower than the preoperative ABG. There were no significant differences between the preoperative and postoperative ABG values in either group. Conclusions: Endoscopic inlay tympanoplasty using the butterfly cartilage technique appears to be an effective alternative to microscopic tympanoplasty and results in excellent hearing.

Evaluation of porcine intestinal organoids as an in vitro model for mammalian orthoreovirus type 3 infection

이세아,Hye Jeong Lee,구나연,박유리,Eun-Ju Kim,Seok-Jin Kang,Bang-Hun Hyun,양동근 대한수의학회 2023 Journal of Veterinary Science Vol.24 No.4

Background: Mammalian orthoreovirus type 3 (MRV3), which is responsible for gastroenteritis in many mammalian species including pigs, has been isolated from piglets with severe diarrhea. However, the use of pig-derived cells as an infection model for swine-MRV3 has rarely been studied. Objectives: This study aims to establish porcine intestinal organoids (PIOs) and examine their susceptibility as an in vitro model for intestinal MRV3 infection. Methods: PIOs were isolated and established from the jejunum of a miniature pig. Established PIOs were characterized using polymerase chain reaction (PCR) and immunofluorescence assays (IFAs) to confirm the expression of small intestine-specific genes and proteins, such as Lgr5, LYZI, Mucin-2, ChgA, and Villin. The monolayered PIOs and three-dimensional (3D) PIOs, obtained through their distribution to expose the apical surface, were infected with MRV3 for 2 h, washed with Dulbecco’s phosphate-buffered saline, and observed. Viral infection was confirmed using PCR and IFA. We performed quantitative real-time reverse transcription-PCR to assess changes in viral copy numbers and gene expressions linked to intestinal epithelial genes and antiviral activity. Results: The established PIOs have molecular characteristics of intestinal organoids. Infected PIOs showed delayed proliferation with disruption of structures. In addition, infection with MRV3 altered the gene expression linked to intestinal epithelial cells and antiviral activity, and these effects were observed in both 2D and 3D models. Furthermore, viral copy numbers in the supernatant of both models increased in a time-dependent manner. Conclusions: We suggest that PIOs can be an in vitro model to study the infection mechanism of MRV3 in detail, facilitating pharmaceutical development.

Computed Tomography and Magnetic Resonance Imaging Evaluation in Pediatric Unilateral Sensorineural Hearing Loss

이세아,이상국,선상우,정재현,이종대,김보경 대한이비인후과학회 2018 대한이비인후과학회지 두경부외과학 Vol.61 No.1

Background and Objectives Children with unilateral sensorineural hearing loss (USNHL) are not actively evaluated by physicians. The diagnostic tool for evaluation of USNHL is also controversial, and no strategy for diagnosing USNHL through imaging studies has been established. We examined the results of temporal bone computed tomography (TBCT) imaging and magnetic resonance imaging (MRI) studies on children with USNHL. Subjects and Method Eighty-nine patients with USNHL were reviewed. Of these patients, 21 underwent both TBCT and MRI, 51 underwent temporal MRI only, and 17 underwent TBCT only. Results The etiology of USNHL were determined through imaging studies in 20 patients. The most common abnormal finding (65%) was a narrow internal auditory canal identified on TBCT and cochlear nerve aplasia on temporal MRI. Incomplete partition (20%), common cavity (10%), and labyrinthitis ossificans (5%) were also observed in imaging studies. The hearing threshold was lower in USNHL patients with normal findings (76.1±28.7 dB) than in USNHL patients with abnormal findings on TBCT or temporal MRI (100.1±22.3 dB). Conclusion Cochlear and cochlear nerve abnormalities can be detected through imaging studies in approximately 25% of patients with USNHL. Therefore, we suggest that children should undergo TBCT when USNHL is confirmed through audiologic evaluation. Korean J Otorhinolaryngol-Head Neck Surg 2018;61(1):9-14

인천국제공항의 독자적인 플랫폼, 트랜스피아

이세아,최민주 한국서비스경영학회 2021 한국서비스경영학회 학술대회 Vol.2021 No.5

전정신경초종과 청력손상

이세아(Se A Lee),이종대(Jong Dae Lee) 대한두개저학회 2020 대한두개저학회지 Vol.15 No.1

Vestibular schwannomas (VSs) are the most common tumors of the cerebellopontine angle. Although VSs arise from the vestibular nerves, 95% of patients with VS present with sensorineural hearing loss (SNHL). The underlying pathophysiological mechanisms of this SNHL are currently unknown. Better understanding of the possible etiologies of hearing loss will help in guiding VS treatment choices based on tumor size and location. The mechanical effect is thought to involve compression or stretching of the cochlear nerve or compromise of the vascular supply to the cochlea and neurotoxic injury caused either by alterations in the biochemical properties of the inner ear fluid or through accumulation of tumor toxic metabolites may be responsible for hearing loss of VS. We review some of these mechanisms with special emphasis on hearing loss associated with VS.