Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

압밀배수공법에 따른 압밀효과 비교 연구

정진호,김근수 釜慶大學校 1999 釜慶大學校 論文集 Vol.4 No.1

The feed-back analysis based both on the in-situ measured values of the settlement of the embankment and on the soil properties of soft clay under the embankment is carried out in this thesis for the Preloading method and the Vertical drains, such as the Pack drains and the P.V.D.. The feed-back analysis is performed with the TCON program which has generally been used to estimate the average degree of consolidation and the final settlement of soft clay under the surcharge. From the results of the feed-back analysis for the final settlement, we can find that there are no significant differences in the behavior of consolidation between the Pack drains and the P.V.D. - the Pack drains show 73.6~75.8% of the average degree of consolidation, the P.V.D., 68~75% and the Preloading method, 48.5~58.7%. Asaoka, Hyperbolic and Hoshino method are also used to predict the final settlement of the embankment ofr the Vertical drains and the Preloading method. The final settlement estimated by the Hyperbolic method appears to be the most close to that by the feed- back analysis.

소아기 외과계질환의 통계적 관찰

정용헌,이재호,이건수,서종진 충남대학교 의과대학 지역사회의학연구소 1989 충남의대잡지 Vol.16 No.2

Child is not little man but rather a father of man. Children are faced to surgical and medical problems reqiring various specialists. Authors analized 1126 pediatric surgical in-patients of Chung-nam National Univ. Hospital from Jan. to Dec. 1988 by departments, age, sex, duration of admission and types of diseases in order to get the information for management of children’s hospital. The results are as follows: 1. Absolute numbers of in-patients by departments are above 200 in general surgery and opthalmology, between 100-199 in plastic surgery, ENT, chest surgery and below 99 in urology and neurosurgery. 2. Male to female ratio as a whole is 1.8:1, male dominant. 3. Duration of admission within 1 week was of 55% as a whole, 87% in ENT, 85% in ophthalmology, 13% in orthopedic surgery. 4. Average duration of admission was 10.8 days as a whole, 4.6 days in ENT, 5.1 in ophthalmology, 29.7 days in orthopedic surgery, and variable by types of diseases. 5. The most frequent disease by departments was hernia in general surgery, fracture in orthopedic surgery, cerebral contusion in neurosurgery, ventricular septal defect in chest surgery, strabismus in ophthalmology, tonsillitis & adenoiditis in ENT.

사람, 집박쥐 및 마우스 파네트세포의 Lysozyme 면역반응성

정진웅,차정호,오수자,전정수 대한해부학회 1991 Anatomy & Cell Biology Vol.24 No.2

국내 아파트 가변형 시스템 적용에 관한 연구

정진원,박진수 경기대학교 산업기술종합연구소 2006 산업기술종합연구소 논문집 Vol.31 No.-

상악 전치부 후방 견인 시 이동 양상과 응력 분포에 관한 삼차원 유한요소법적 연구

정애진,김운수,이수행,강성수,최희인,조진형,김상철 대한치과교정학회 2007 대한치과교정학회지 Vol.37 No.2

상악 전치부 후방 견인 시의 이동 양상 및 응력 분포를 건조 두개골을 전산화 단층 촬영에 의해 3차원 영상화한 유한 요소 모델 상에서 알아보고자 하였다. 피질골 절단술의 시행 여부와 고정원, 힘의 작용점을 각기 달리 설정하여 8개의 실험군을 구성하여 비교하였다. 통상적인 T-loop을 이용하여 공간폐쇄를 하는 경우 전치부는 후하방으로 경사이동 하였으며, 구치부에서도 약간 전방 이동하였다. 피질골 절단술을 동반하여 전치부를 견인한 경우, 전치부 골편에서의 응력 분포가 전반적으로 넓게 분포되었으며, 전치부 경사의 정도가 적은 반면에 변위량은 훨씬 더 많았다. 협측에서의 견인 시 상악 협측에 식립된 미니 임플랜트와 견치 power arm간에 견인력을 가한 경우가 미니 임플랜트로 고정원이 강화된 제2소구치와 견치 브라켓 간에 견인력을 가한 경우보다 전치부의 후하방 경사 정도가 적었다. 구개측에서 power arm에 대한 견인 시 정중구개봉합 부위에 식립한 미니 임플랜트로부터 견인력을 가한 경우가 상악 제1, 2대구치 간 구개면에 식립된 미니 임플랜트로부터 견인력을 가한 경우보다 전치부의 후하방 경사 정도가 컸다. 이러한 결과로써 치아이동 시 피질골 절단술의 효과와 저항중심에 대한 교정력 벡터 조정의 의미를 확인할 수 있었다. Objective: The purpose of this study was to evaluate the displacement pattern and the stress distribution shown on a finite element model 3-D visualization of a dry human skull using CT during the retraction of upper anterior teeth. Methods: Experimental groups were differentiated into 8 groups according to corticotomy, anchorage (buccal: mini implant between the maxillary second premolar and first molar and second premolar reinforced with a mini implant, palatal: mini implant between the maxillary first molar and second molar and mini implant on the midpalatal suture) and force application point (use of a power arm or not). Results: In cases where anterior teeth were retracted by a conventional T-loop arch wire, the anterior teeth tipped more postero-inferiorly and the posterior teeth moved slightly in a mesial direction. In cases where anterior teeth were retracted with corticotomy, the stress at the anterior bone segment was distributed widely and showed a smaller degree of tipping movement of the anterior teeth, but with a greater amount of displacement. In cases where anterior teeth were retracted from the buccal side with force applied to the mini implant placed between the maxillary second premolar and the first molar to the canine power arm, it showed that a smaller degree of tipping movement was generated than when force was applied to the second premolar reinforced with a mini implant from the canine bracket. In cases where anterior teeth were retracted from the palatal side with force applied to the mini implant on the midpalatal suture, it resulted in a greater degree of tipping movement than when force was applied to the mini implant between the maxillary first and second molars. Conclusion: The results of this study verifies the effects of corticotomies and the effects of controlling orthodontic force vectors during tooth movement.

은행의 부실채권 처리 방안에 관한 연구

정수원,박태진 東西大學校 1998 동서논문집 Vol.4 No.-

The purpose of this paper is to examinate of the Financial Structure Reform in Korea and Japan. Through examination of the Financial Structure Reform in Japan, the suggestions to Korea is as follows. 1. To securitization of insolvency assets is better than injection of government fiscal funds. 2. We have to establish rule of the Financial Structure Reform. 3. We have to stop "too big to fail" and have to readjust insolvency big banks. 4. We have to strengthen of supervisory function on an enterprise by banks.

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

PLD 박막의 물리적 성질

한용진,조봉균,정재훈,이수빈,박해윤,이태기,채희백,홍진수 순천향대학교 기초과학연구소 1998 순천향자연과학연구 논문집 Vol.4 No.2

Thin amorphous carbon films were deposited by a Q-switched Nd;YAG 532nm with beam power density of ?? on the high purity graphite (99.7%). The pressure of vacuum chamber was ?? Torr. In order to estimate the quality of the Pulsed Laser Deposition films one of the most important optical properties, bandgap energy, was characterized by transmission and reflection in the range of the visible, and an optical direct bandgap energy of 2.45eV and indirect bandgap energy 0.36eV were obtained. Surface morphology of amorphous film was investigated by AFM (Atomic Force Microscopy). Its surface roughness is 70nm. Comparing our results with the published values in the literature we have confidence that our films show the good quality for optical properties.

다발성 골수종에서 저용량 thalidomide, cyclophosphamide, dexamethasone (TCD) 요법의 효과

류충헌,정재현,고정해,장제혁,박영진,최규남,박봉수,이상민,주영돈 인제대학교 2008 仁濟醫學 Vol.29 No.-

Background and Objectives : The immunomodulatory drug thalidomide can inhibit angiogenesis and induce apoptosis in experimental models. It can also induce marked and durable response in newly diagnosed myeloma patients. Thalidomide has been used at doses ranging from 200 to 800 mg with significant toxicity. No data are available on the impact of low-dose thalidomide, cyclophosphamide and dexamethasone as initial therapy for myeloma patients. Design and Methods : To address this issue, newly diagnosed myeloma patients were treated with 50 mg/day thalidomide continuously and cyclophosphamide 150 mg/m², days 1-4 and dexamethasone 20 mg/m², days 1-5 and day 15-19, every month. Between October 2005 and October 2006, 14 patients (median age 54.5 years) were treated with low-dose thalidomide, cyclophosphamide and dexamethasone. Results : After a minimum of two cycles of treatment, 5 patients (55.5%) showed a partial remission. After four cycles of treatment, 10 patients (83.3%) showed a partial remission (n=6) and complete remission (n=4). After a median follow-up of 15.4 months, 1 year overall survival rate was 82.0%. Thalidomide was well tolerated without serious toxic effects. Conclusions : The combination of low-dose thalidomide, cyclophosphamide and dexamethasone demonstrates favorable response rate and 1 year overall survival rate in newly diagnosed myeloma. Severe toxicities were not seen with this combination.