군사시설 이전부지 공원화 사례분석을 통한 시사점 도출

박준영,이은엽,송선영,엽정식,Park, Joon-Young,Lee, Eun-Yeob,Song, Sun-Young,Yeob, Jung-Sik 한국토지주택공사 토지주택연구원 2014 LHI journal of land, housing, and urban affairs Vol.5 No.4

군사시설 이전부지는 근본적으로 공공 시설적 성격을 띄고 있어 공공 복리를 위한 활용 요구도가 높아지고 있으며, 이로 인해 공원으로 조성하는 사례가 증가하고 있다. 군사시설 이전부지에 대한 공원조성 사례를 분석한 결과 몇가지의 시사점을 도출하게 되었다. 공원조성의 목표는 도시재생의 개념 및 지역활성화 차원에서 접근할 필요가 있다. 이전부지를 전면 개조하는 방식보다는 기존 시설들을 최대한 재활용하여 경제적 이득을 취하고 동시에 지역정체성과 역사성을 재현할 필요가 있다. 공원의 규모가 대형화 되고 있어 단일시설 및 프로그램 보다는 복합공간으로 조성해 줄 필요가 있으며, 공원조성에 막대한 비용이 소요되므로 단계적으로 공원을 조성하는 방안을 고려할 필요가 있다. 군사시설이 지니고 있는 특수한 상황으로 공원조성 과정에서 현장조사가 미흡하여 설계변경 또는 오염처리에 막대한 비용이 소요되므로 사전에 이를 고려할 필요가 있다. 또한, 민간사업자에게 일부 부지에 대해 개발권을 위임하고 개발이익으로 공원조성과 운영관리비를 충당하는 방안도 검토가 필요하다. 군사시설 이전기지는 다양한 이해관계에 놓여 있으므로 사업추진과정에서 민관협치의 의사결정기구를 운영하는 방안을 고려할 필요가 있다. 다양한 재원조달과 수익사업 확대, 봉사 및 후원 활성화, 트러스트 및 비영리 단체의 공원운영 관리 참여 확대 등을 위해서는 도시공원 관련제도의 보완이 요구된다. Because of their nature as public facilities, demands that former military facilities be utilized for the public welfare are increasing, thereby leading to an increase in cases in which these military facilities are reestablished as parks. Cases in which former military bases were reestablished as public parks were analyzed; as a result, several implications were derived. First, the objectives of public park projects should be examined from the perspective of the concept of urban regeneration and regional revitalization. Moreover, it is necessary that profits are yielded and that regional identity and history are reproduced through reusing existing facilities as much as possible rather than entirely remodeling former military sites. As parks become larger in size, bases should be reorganized into complexes rather than single facilities or programs. It is also necessary that parks be established in stages considering the enormous expenses required for building public parks. Consequently, because the special characteristics of military facilities can lead to insufficient on-site investigation in the process of establishing parks, thereby incurring a vast amount of costs for design adjustment and contamination disposal, this should be considered in advance. A method of delegating the development rights to partial sites to private businesses and supplementing the costs of park establishment and maintenance with development benefits should be examined. In addition, given that there are various interests and stakes in former military bases, a method of operating a public-private cooperative decision-making organization during project execution should be considered. Finally, policies related to urban parks need to be improved in order to raise funds, expand profitable businesses, facilitate social services and sponsorship, and encourage the participation of trusts and non-profit organizations in park operation and management.

Protein kinase A-induced phosphorylation at the Thr154 affects stability of DJ-1

Ko, Yeon Uk,Kim, Su-Jeong,Lee, Juhyung,Song, Min-Young,Park, Kang-Sik,Park, Jun Bae,Cho, Hyun-Soo,Oh, Young J. Elsevier 2019 Parkinsonism & related disorders Vol.66 No.-

<P><B>Abstract</B></P> <P><B>Introduction</B></P> <P>Most cases of Parkinson's disease (PD) are sporadic, but genetic variations have been discovered in PD patients. PARK7/DJ-1 is a known cause of early-onset autosomal-recessive PD and is implicated in neuroprotection against oxidative stress. Although several post-translational modifications of DJ-1 have been proposed, phospho-modification of DJ-1 and its functional consequences have been less studied.</P> <P><B>Methods</B></P> <P>Putative phosphorylation sites of DJ-1 were determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS analysis). Subsequently, phosphorylation site of DJ-1 was confirmed by <I>in vitro</I> kinase assay and cell-based pull-down assay. Impaired dimer formation of phospho-null mutant was measured using DSS crosslinking assay and immunoprecipitation assay. To evaluate physiological consequences of this event, protein stability of DJ-1 WT and DJ-1 phospho-null mutant were compared using cycloheximide chase assay and ubiquitination assay.</P> <P><B>Results</B></P> <P>Here, we showed that DJ-1 directly bound to the catalytic subunit of protein kinase A (PKAcα). We found that PKAcα is responsible for phosphorylation of DJ-1 at the T154 residue. Interestingly, dimerization of DJ-1 was not detected in a DJ-1 T154A mutant. Furthermore, stability of the DJ-1 T154A mutant was dramatically reduced compared with that of wild-type DJ-1. We found that DJ-1 T154A was prone to degradation by the ubiquitin proteasome system (UPS).</P> <P><B>Conclusion</B></P> <P>We identified a novel phosphorylation site of DJ-1. Furthermore, we determined protein kinase A that is responsible for this posttranslational modification. Finally, we demonstrated physiological consequences of this event focusing on dimerization and protein stability of DJ-1.</P> <P><B>Highlights</B></P> <P> <UL> <LI> PKAcα phosphorylates PARK7/DJ1 at the Thr154 residue. </LI> <LI> Disruption of Thr154 phosphorylation is linked to a lower stability. </LI> </UL> </P>

軟弱地盤 壓密特性과 沈下豫測

이재식,류제천,김송만,박춘수 충남대학교 산업기술연구소 2001 산업기술연구논문집 Vol.16 No.1

Recently, developed countries have brought about a considerable increase in the study of soft ground for the reasons of policy on farming protection, key industry and sightseeing development since the middle of the nineteenth century, particularly 1950s. In spite of the long and copious experience associated with these types of construction, the constructions of soft ground still have many delicate problems. In general, problems of soft ground related to stability are probably to meet settlements on the various circumstances. In order to accelerate the rate of consolidation settlement and gain a required shear strength for a given soft ground, the preloading technique combined with a vertical drainage system has been widely applied. In this paper, laboratory tests have analyzed to change the soil parameters that executed the plan of stage construction. The void ratio and water content of the clay decreases and the strength of soft ground rises during consolidation period. In order to predict of magnitude, rate of settlement and pore pressure dissipation under embankment of loading on drainage-installed soft ground, consolidation settlement prediction methods such as Asaoka method, curve fitting method, TAN method and Hyperbolic method applied to gain final consolidation settlement from the field settlement data. The Reliability analysis of consolidation settlement prediction methods made use of additional settlement data. Sequentially, it has been found that Hyperbolic method can appropriately predict the consolidation behavior of drainage-installed soft ground of Yangsan Mulgum.

카드뮴투여가 흰쥐 고환의 조직변화와 단백질변화에 미치는 영향

최송표,김수일,조근자,양은진,김선,박경란,이영호,김원식 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.2

This study was done to investigate histochemical and protein changes in the rat testis I WAT and 3 WAT with 4 mg/kg of cadmium, IP, single injection. A control group and 2 experimental groups were studied. Testes were processed routinely for H&E stain and agarose gel electrophoresis. The results were as follows ; 1. In the histochemical studies, death of all the spermatogenic cells and Sertoli cells in the seminiferous tubules, and decreased ground substance, decreased fibroblasts and interstitial cells and increased inflammatory cells in the interstitial tissues were found in the cadmium treated groups, and the degree of necrotic changes was more severe in the 3 WAT group. 2. In the biochemical studies, two kinds of proteins, 25 kDa and 45 kDa, were disappeared and one kind of protein, 100 kDa, was decreased after Cd treatment. 25 kDa and 45 kDa proteins were suggested as actin which consist the spermatogenic cells, but what's the protein 100 kDa is unclear. According to the above results, it is considered that there would be irreversible changes in seminiferous convoluted tubules and also in the interstitial tissues after cadmium treatment, and detecting amino acid sequences of these proteins would help understanding the mechanism of necrosis in the testis tissue after cadmium administration.

여성 정신지체인의 취업에 대한 가족의 태도 연구 : 부산 ·경남지역을 중심으로

김기태,박병현,최송식 釜山大學校 社會福祉硏究所 1999 社會福祉硏究 Vol.9 No.-

미만성 경화 아형 갑상선 유두암 1예

최정민,이송주,김부경,최영식,박요한,김정훈,허방 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.1

The diffuse sclerosing variant (DSV) is a rare malignant tumour that accounts for 0.7-5.3% of all papillary thyroid cancers. DSV tends to occur at a younger age and has a higher incidence of cervical lymph node metastases, compared with papillary thyroid carcinoma. A 62-year-old female patient diagnosed thyroid nodule with diffuse and mild increased 18F-deoxy-glucose (FDG) on FDG positron emission tomography (PET)-computed tomogram (CT) scan. The ultrasound images showed typical findings of DSV such as diffusely enlarged thyroid lobes and diffusely scattered microcalcification. We performed this patient ultrasonography guided fine needle aspiration and cytologic analysis revealed papillary carcinoma. Total thyroidectomy with central compartment node dissection and lateral lymph node dissection was performed and finally diagnosed DVS. The authors report this case with literature review.

Chlorpromazine이 백서 해마에서 전기경련 충격에 의한 MAPK 타이로신 인산화에 미치는 영향

주연호,정성훈,전송희,강웅구,김용식,김상욱,박주배 大韓神經精神醫學會 1997 신경정신의학 Vol.36 No.2

Object : In order to examine the interaction mechanisms of electroconvulsive shock(ECS) and antipsychotic drug at the level of molecular biology, we observed the effect of chlorpromazine pre-treatment on the activation of mitogen activated protein kinase(MAPK) induced by electroconvulsive shock(ECS) in rat hippocampus Method : Male Sprague-Dawley rats were divided into 2 groups. To the experimental group chlorpromazine(20㎎/㎏) was given intraperitoneally, and to the control distilled water was given instead. Thirty minutes later, ECS was given and the hippocampus was dissected out 2 minutes thereafter. Immunoblotting with antiphosphotyrosine antibody was carried out, and the signal intensity at 42kDa band was quantitized using densitometer. The obtained result was compared by student t-test between the experimental and the control group. The absolute amount of MAPK was measured by immunoblotting with anti-MAPK antibody. Result : The tyrosine phosphorylation of MAPK reached peak at 2 minutes after ECS. However, in the chlorpromazine pre-treated group, the peak level of MAPK tyrosine phosphorylation was significantly attenuated(t=-3.12, df=14, p=0.008) compared to the control. In contrast to this, the absolute amount of MAPK did not differ between the pretreated and the control group. Conclusion : Chlorpromazine attenuated the tyrosine phosphorylation of MAPK by ECS. This finding seems to be related to the fact that chlorpromazine pre-treatment changed the cfos expression by ECS in rat brain. Antipsychotic drug and ECS might interact at the level of MAPK signal transduction system, and this might explained the observed synergistic effect of two treatment modality.

지문과 정신분열증

우숙희,정국동,최송표,조근자,양은진,김수일,박경란,이영호,김원식 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

Fingerprint patterns of 333 schizophreniacs who met with the diagnostic criterias of International Pilot Study of Schizophrenia except items associated with chronicity in exclusion criteria were studied, and the following results were obtained. 1. The frequency distribution of the fingerprint patterns in the 333 schizophreniacs was ulnar loop, whorl, twinned loop, arch, radial loop in order in both control and schizophrenia groups. 2. Finger ridge counts of both sexes were decreased significantly in schizophrenia group. 3. Dissociation of fingerprint pattern in the schizophrenia group was 51.1%, which was far greater than 6.25% of control group. Number of dissociation was greatest in the thumb, followed by index, middle, ring, and little finger in order. According to the above results, it is considered that both the number of finger ridges and degree of dissociation of fingerprint patterns were closely related with schizophrenia. Analysis of fingerprints and palmprints with the aid of chromosomal analysis would contribute the early diagnosis and prevention of schizophrenia.

흰쥐 뇌에서 전기경련 충격에 의한 CREB 인산화의 발달단계에 따른 변화

강웅구,정희연,안용민,정선주,전송희,박주배,조수철,김용식 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.3

연구목적 : 이 논문에서는 뇌에서 전기경련 충격(Electroconvulsive shock. ECS)에 의한 전사인자 CREB의 인산화를 발달단계에 따라 알아봄으로써 정신장애의 신경발달학적 이해를 위한 생물학적 기반지식을 얻고자 하였다. 방 법 : 생후 7. 14. 21일 및 성년 흰쥐에 ECS를 가하고 시간별로 해마 및 소뇌 조직을 얻어 CREB의 인산화를 알아보기 위해 특이 항체로 면역블롯을 실시하였다. 결 과 : 발달단계에 따라 해마에서는 CREB이 감소한 반면 소뇌에서는 CREB이 증가하였다. 기저상태의 CREB 인산화는 해마와 소뇌에서 생후 7일에 비해 14일 이후 증가하였는데, 소뇌의 경우 CREB의 양증가와 비례하였다. ECS 후 CREB인산화 증가는 해마에서는 생후 21일 이후에 나타났으나, 소뇌에서는 생후 7일은 물론 성년에서도 나타나지 않았다. 결 론 : CREB 매개 신호전달은 발달단계별 및 조직별 활성의 차이를 나타내었으며 해마에서는 생후 21일 이후 ECS에 의해 활성화되었지만, 소뇌에서는 그렇지 않았다. 해마에서 ECS에 의한 CREB 인산화 증가는 c-fos 유전자의 발현과 관계있으리라고 생각되지만, CREB의 Ser-133 인산화 만으로는 발달단계 및 조직에 따른 c-fos 발현의 특이성을 설명할 수 없었다. Objectives : In order to understand the biological basis of neurodevelopmental perspectives of mental disorders, the authors investigated the developmental and regional changes in the phosphorylation of the transcription factor CREB following the electroconvulsive shock(ECS) in rat brain. Methods : Rats of various age groups(7, 14, 21 days postnatal and adults) were given ECS and their hippocampi and cerebella were dissected at specified time points. The content of CREB and phosphorylated CREB were measured by immunoblot analysis. Results : The amount of CREB increased in the hippocampus and decreased in the cerebellum according to the age. Baseline levels of CREB phosphorylation in both tissues were increased from postnatal 14 days, and it was proportional to the amount of CREB protein in the cerebellum. In the hippocampus, ECS increased the phosphorylation of CREB at postnatal 21 days, but in the cerebellum, ECS did not increased the phosphorylation of CREB in any age group. Conclusion : CREB mediated signal transduction pathways showed developmental and tissue-specific changes. ECS increased the phosphorylation of CREB in the hippocampus by postnatal 21 days, but not in the cerebellum. CREB activation is supposed to be related with the induction of c-fos after ECS in the hippocampus. However, the Ser-133 phosphorylation of CREB could not completely explain the developmental and tissue specificity of c-fos induction.

A New Forage Barley Cultivar with Semi-Smooth Awn and High Yielding ‘Miho’

Young-Jin Oh,Tae-Il Park,Hyoung-Ho Park,Ouk-Kyu Han,Jong-Chul Park,Tae-Hwa Song,Yang-Kil Kim,Hyeon-Jung Kang,Jae-Seong Choi,Yun-Woo Jang,Kwang-Geun Park,Jong-Ho Park,Chon-Sik Kang,Young-Keun Cheong,Ky 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

The purpose of development new variety ‘Miho’ (Hordeum vulgare L.) is a favorite with livestock feed and develop varieties resistant to disease and lodging. ‘Miho’ was carrying the growth habit of group Ⅲ, green and mid-wide leaf. Awn that are related to preference of livestock is a semi-smooth awn. This cultivar had 96cm of culm length, 650 of spikes per m2. Heading date of ‘Miho’ is April 27, and maturing dates on May 30, which were later than cultivar ‘Youngyang’. It also showed strong winter hardiness, and similar resistance to shattering and BaYMV compared with those of check one. The best thing among the traits of one is a new good quality with the plant green at the latter growing period. The average forage dry matter yield in the regional yield trial was about 13.1, 12.1 MT per ha in upland and paddy field, respectively, which were 9%, 2% higher than that of the check cultivar. It’s also showed 6.8% crude protein, 27.1% ADF (acid detergent fiber), and 67.5% TDN (Total Digestible Nutrients), including higher silage quality for whole crop barley. This cultivar would be suitable for the area whose daily minimum temperature was above -8℃ in January in Korean peninsula.