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화학적 손상에 따른 간 재생의 '간 조상세포' 동태에 관한 연구
허방,박명선,윤명희,이충한 고신대학교(의대) 고신대학교 의과대학 학술지 2004 고신대학교 의과대학 학술지 Vol.19 No.1
Background : Oval (OV) cells had been described as an uncertain type of cells in early neoplastic process of animal hepatocarcinogenesis model. Although biological significance of OV cells is not elucidated in human, they showed cholangiolar and hepatocytic bipotential differentiation in animals, especially rodents. With recent studies emphasizing on a possible target of gene therapy and cell transplantation of hepatic stem cells, biological investigation of OV cells become to be an important issue. In present study, the authors pursue the fate of hepatic stem cell progenitor cells such as OV cells using 2-acetylaminofluorene (AAF)/partial hepatectomy (PH) of resistant hepatocyte (RH) model. Materials & Methods : Through gastrostomy tube, 2-AAF (10mg/kg) was administrated daily for 10 days in SD rats. Seventy percent PH was performed at 5th day of 2-AAF treatment. Animals were killed 1,3,5,7,9 and 14 days following the last day of 2-AAF. Bromodeoxyuridine (BrdU) (100mg/kg) was given intraperitoneally 1 hour before killing in each experiment. Hematoxylin eosin-stained histological sections and morphometric changes were analysed by microscope (Americal Optical microStar) with right angle section of portal regions by means of unit areas of high power view. For BrdU incorporation, the sections were immunostained by an anti-BrdU antibody and labelled nuclei were counted. For BrdU incorporation, the sections were immunostained by an anti-BrdU antibody and labelled nuclei were counted. For electron microscopy, fresh liver tissues fixed with 2% glutaraldehyde were processed by routine method. Results : 1)It was confirmed that periportal OV cells are a major component of AAF/PH-induced hepatic regeneration based on OV cell proliferation in concord with restoration of liver weight. 2) With BrdU incorporation, OV cell proliferative activity migrates toward pericentral zone from periportal zone. 3) OV cells show bile ductular differentiation with progressive regression and later hepatocytic differentiation through basophilic small hepatocytic transition. 4) In contrast to OV cells, basophilic small hepatocytes emerged from periphery of OV cell proliferative zone in late period of regeneration, inspite of independent pericentral localization of small proportion. 5) Ultrastructurally 3 type of OV cells, at least, were noted in periportal proliferative zone; predominant biliary differentiated cells, sparce undifferentiated small cells and hepatocytic transitional cells. 6) Colonized hematopoietic cells with a few blast-like cells were noted following OV cell proliferation chiefly in areas of periphery of OV cell proliferative zone. Conclusions : It was confirmed that AAF/PH is a hepatic regeneration model through OV cell proliferation. In this model, hepatic regeneration could be accomplished with periportal OV cell proliferation and subsequent hepatocytic differentiation via small basophilic hepatocytic transition, in addition to a possible role of extramedullary hematopoietic cells.
허방,허만하 고신대학교(의대) 고신대학교 의과대학 학술지 1987 고신대학교 의과대학 학술지 Vol.3 No.1
Chordoma is a rare, slow-growing, but relentless tumor which arises from primitive notochodal remnants. This tumor is a locally aggressive, rarely, if ever, metastasizing tumor. Although it occurs primarily in the spheno-occipital and sacrococcygeal regions, it may occasionally present with a nasopharyngeal mass in the intervening vertebrae of the cervical spine such as this case. The treatment of choice is a surgical excision with radiation therapy. The authors experienced a rare case of pathologically confirmed cervical chordoma in a 65-year-old Korean female who had suffered from dysphagia for several months. We report the case with literature review with emphasis on the histogenesis of chordoma and clinopathologic features.
자궁 내막 선암종을 동반한 Stein-Leventhal 증후군 - 1증례 보고 -
허방,전인선,허만하 고신대학교(의대) 고신대학교 의과대학 학술지 1990 고신대학교 의과대학 학술지 Vol.6 No.1
Stein-Leventhal first described a syndrome consisting of amenorrhea, infertility, and a pathologic feature of bilateral polycystic ovaries. This syndrome is not infrequently associated with endometrial hyperplasia or carcinoma. Through a number of possible theories regarding etiologic factors have been explored, the pathogenetic mechanisms are including hypothalamus-pituitary axis, adrenal factors, and ovarian steroidogenesis. The authors present a case of Stein-Leventhal syndrome associated with endometrial adenocarcinoma in a 30-year-old female, who complained of intermittent vaginal bleeding for 10 years and infertility for 5 years of marriage. A diagnostic endometrial curettage shwed grade 1 endometrial adenocarcinoma. Total hysterectomy with bilateral salpingo0oophorectomy was performed. Both ovaries revealed symmetrical enlargement and disclosed multiple subcapsular cystic follicles. Numerous primodial follicles were noted. However, corpus luteum and albicans were not identified. The endometrial mucosal surface showed irregular mucosal elevation which was confirmed to be endometrial adenocarcinoma. We report this case in view of its rarity and academic interest along with a literature review on the pathogenesis.
허방,허만하 고신대학교(의대) 고신대학교 의과대학 학술지 1989 고신대학교 의과대학 학술지 Vol.5 No.1
Localized fibrous mesothelioma of pleura is a relatively uncommon tumor of which histogenesis of the tumor cells is still controversial. It remains uncertain whether this tumor takes origin from mesothelial cells or submesothelial connective tissue. Although some of tissue culture and electron microscopic studies reveal both epithelial and fibroblastic nature, others, employing immunohistochemical studies, fail to reveal an evidence of epithelial characteristics. The authors report a typical case of histologically benign form of localized fibrous mesothelioma of the night pleura in a 54-year-old Korean female along a brief review of some related literatures with a special reference on the histogenesis of the tumor cells. This is the second reported case of benign localized fibrous mesothelioma of pleura, followed by the report of Kwon etal(1984) in Korea.