Application of Artificial Insemination Technology for Dairy Breeding in Mongolia

Hyun‐Tae Jo,Jong‐In Jin,Seong‐Su Kim,Byung‐Hyun Choi,Tumor Baldan,Jung‐Gyu Lee,Yun‐Shik Kim,Sam‐Churl Kim,Il‐Keun Kong 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1

This study was focused on improvement of milk production in Mongolia dairy industry by artificial insemination (AI) technology, of which was supported from ODA project of KOICA in Republic of Korea. The study was started from January 2009 to present and 3rd years in this year. So, all data, especially synchronization and pregnancy of dairy cows (Holstein) will be summarized in final result in this year. For synchronization, total 81 dairy cows selected from 4 private farms that were 38, 30, 8 and 5 in Undarmal milk, Onjin (Enkhbayer), Jargalant, and BRM School, respectively. All the dairy cows were injected intramuscular (IM) of 5 ml PGF2α in the vulva and detected estrus 2 to 3 days after PGF2α injection. Total 78 out of 81 dairy cows (96.3%) were detected estrus by only 1 time injection of PGF2α. The dairy cows that were induced estrus, inseminated with 0.5 ml dairy frozen semen by conventional AI techniques. The pregnancy diagnosis of the AI dairy cows was detected by uterus palpation after 60 days of insemination. Total 75 from 78 inseminated dairy cows (90.1%) were diagnosis pregnant. The estrus induction and pregnant rate were very effective using PGF2α injection and conventional AI techniques in Mongolia dairy cow. The results indicated that AI after estrus induction in Mongolia dairy cows could be applied to dairy breeding technology to improve the breeding efficiency and milk production.

Comparison of Hanwoo with Holstein of OPU derived Embryo and Offspring Production

Jong‐In Jin,Byung‐Hyun Choi,Seong‐Su Kim,Hyun‐Tae Jo,Il‐Keun Kong 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1

The objective of this study was to evaluate the comparison of production efficiency of oocytes and OPU (ovum pick‐up) derived embryos of Hanwoo with Holstein. The OPU session of each species (6 cows) was carried out from the Hanwoo (106 sessions) and Holstein (114 sessions) at intervals of 3 4 days (2 times per week) for 3 months. Cumulus‐oocyte‐complexes (COCs) retrieved were classified into 4 grades by the status of oocyte cytoplasm and cumulus cells. The COCs collected were matured in vitro in TCM‐199 supplemented with 10% FBS, 10 mg/ml FSH and 1 mg/ml estradiol‐ 17β in 5% CO2 and over 99% humidity for 24 h. After 24 h co‐incubation with post‐thaw sperm, the presumed zygotes were cultured in CR1aa medium with 4 mg/ml BSA for 3 days and then changed CR1aa medium with 10% of FBS for another 3 4 days. The Mean number of aspirated follicles and collected oocytes were not significantly different between Hanwoo and Holstein spacies (10.4±0.42 vs. 11.4±0.41 and 7.5±0.38 vs. 6.1±0.37 per session). But the collection rate of oocytes from aspirated follicles were significantly higher in Hanwoo (72.8%) than that in Holstein (53.6%) (p< 0.05). Furthermore, the average number of good quality oocytes (Grade I and II) was 5.9±0.28 and 4.1±0.27 (Mean±SD), and the cleavage rate and the development rate to blastocysts was significantly (p<0.05) higher in Hanwoo (40.0%) than Holstein (21.6%). The OPU derived embryos of Hanwoo were transferred 83 times into 52 recipients and then 42 calves were produced from 44 pregnancy recipients. In conclusion, the efficiency of OPU derived embryo was significantly different between Hanwoo and Holstein species. In vitro culture system for OPU derived embryo production should be optimized for industrialization and the improvement of livestock.

조혈모세포이식 환자에서 침습성 진균 감염에 대한 Micafungin의 예방 효과 및 안전성

김시현,이동건,최수미,권재철,박선희,최정현,유진홍,이성은,조병식,김유진,이석,김희제,민창기,조석구,김동욱,이종욱,민우성,박종원 대한감염학회 2010 감염과 화학요법 Vol.42 No.3

Background: Micafungin, a potent inhibitor of 1,3-β-D-glucan synthase, is a novel antifungal agent of the echinocandin class. In vitro study showed that micafungin was effective against Aspergillus species as well as Candida species, but clinical data on the prophylactic efficacy against invasive fungal infections (IFIs) other than candidiasis are still lacking. Materials and Methods: We identified 60 consecutive adult hematopoietic stem cell transplantation (HSCT) recipients who received at least 3 doses of micafungin during neutropenic period. Micafungin was started as an alternative in patients who were intolerant or had adverse events (AEs) to primary prophylactic antifungal agents. We retrospectively reviewed the medical records and analyzed the efficacy and safety of micafungin for prophylaxis against IFIs. Results: The patients either had autologous (n=9) or allogeneic (n=51: 1 syngeneic, 24 sibling, 26 unrelated donor) HSCT. Itraconazole oral solution (n=58) was the most frequently used first line antifungal agent for prophylaxis and was administered for median 11 days. The most frequent cause of switch to micafungin was vomiting (n=42). The duration of neutropenia and micafungin administration was median 13 and 12 days, respectively. A successful outcome was achieved in 45 (75%) patients. Empirical antifungal therapy was initiated in 13 (22%) patients. There were 2 cases (3.3%) of breakthrough fungal infections which comprised a probable invasive pulmonary aspergillosis and a possible invasive fungal sinusitis. There was no case of invasive candidiasis. A total of 53 (88%) patients experienced at least one AE regardless of causality during micafungin administration. The most frequent AEs were hypokalemia, vomiting, diarrhea, and elevated serum aspartate aminotransferase or alanine aminotransferase. Among the aforementioned AEs, only 1 case of diarrhea could be classified as a probable relation with micafungin when causality was assessed. There was no AEs that caused discontinuation of micafungin. Conclusions: Micafungin seems to be a safe and effective agent for prophylaxis of IFIs including aspergillosis as well as candidiasis in HSCT recipients. However, further large, prospective, and randomized comparative studies are warranted for aspergillosis.

老齡者의 建康을 위한 生活習慣 實態調査

申東敏,長鳳愚,李揆文,金賢俊,金昌範,崔宗洙,崔鍾晥,李鍾珏,金圭碩,趙庚旭,金鍾聲,朴鍾振 平生體育硏究所 論文集 1987 平生體育硏究所 論文集 Vol.2 No.-

With a view to examining the habit and method in which old people maintain and improve their health, we consulted 551 men and 444 women who were over 65 years old, and concluded as follows: 1. Habit of eating It's been revealed that most of the old people keep a regular eating hour, control the quantity of meal or eat a little food, and prefer vegetables to meat. 2. Mental health Old people feel the necessity for mental health and most of them (about 80%) keep good mental health without becoming pessimistic. Their chief concenrs have turned out to be about their sons and daughters and their own health and finance. 3. Managing Good Health It has been revelaed that old people do not possess any special kind of secret to keep good health but taking plenty of rest, regular meal, and proper exercise, and many of them (about 60%) have been shown to eat invigorant food that they can easily get around their living area. 4. Physical Functions It has been revealed that many old people are conscious of some symptoms about their parts of body and about to percent of them suffers from neuralgia. Some measures like medical aid especially for old people need to be taken as soon as possible. As we have found that the health-keeping method of old people consists plenty of rest, proper exercise, and appropriate nutrition, we can conclude that the recreatonal and culture facilities and programs for keeping old people in good health should be developed. It is also necessary to study food that contribute to their health and develop ways to get rid of the stresses to which they are exposed in their daily life. Presides, it is natural that government found an administrative department to deal with these matters effectively.

안면 마비가 동반된 톨로사 헌트 증후군

강성진,김광수,이지현,김종국,김민정 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.1

A 21-year-old woman developed painful left third, fourth and sixth nerve palsy. The retro-orbital pain and ophthalmoplegia were improved after steroid pulse therapy. After six weeks, during took steroid by orally left facial weakness developed. Neurologic examination showed left peripheral seventh cranial nerve palsy. The Tolosa-Hunt syndrome associated with facial palsy is rare condition. So we report a case of Tolosa-Hunt syndrome preceded by ipsilateral facial palsy

Effects of Pilger Rolling and Heat Treatment on Microstructure and Corrosion Resistance of Ag-electroplated 304 Stainless Steel Tubes

Hyun Park,Woo‑Jin Lee,Jae‑Han Son,Han‑Kyun Shin,Sung‑Kyu Hong,Hyo‑Jong Lee 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.8

We introduce a cost-effective method that combines electroplating with metal cold working processes to manufacture compositetubes with robust corrosion resistance. First, a 3-step electroplating process was developed to form an adhesive anduniform Ag coating on the outer wall of a 304 stainless steel tube. The process consisted of a Ni-strike step for removing thepassivation layer of the initial 304 tube, Cu deposition for smoothing the surface by adding a buffer layer, and Ag deposition. To reinforce the interfacial adhesion between the electroplated layers and the 304 tube and increase the area of the Ag coating,a pilger rolling or pilger rolling + heat-treatment process was performed after the Ag electroplating process. Scanningelectron microscopy of the composite tubes after each process indicated that the as-deposited Ag coating changed fromhaving a rough to smooth surface after only pilger rolling and with additional heat treatment. Electron backscatter diffractionanalysis of the microstructures and textures of the tubes revealed that dynamic recrystallization occurred extensively duringpilger rolling, resulting in the formation of a relatively defect-free grain structure without heat treatment. Furthermore,electrochemical polarization curves determined that the Ag-coated composite tubes are superior to the uncoated 304 tubein terms of corrosion resistance under Cl−atmosphere, owing to the formation of a AgCl passivation layer during testing. Surface analysis of the composite tubes suggests that the properties of the AgCl passivation layer are related to the grainsize of the Ag coating and the density of plastic-deformation-induced defects.

Human Cytomegalovirus 감염시 UL47 promoter 활성의 분석과 Escherichia coli에서 발현되는 UL47 단백질의 분석

현종준,김기호,김홍진 중앙대학교 약학연구소 1997 약학 논총 Vol.11 No.-

In order to study of HCMV(human cytomegalovirus) UL47 promoter activity, we have used the transient transfection assay method. We cloned the -20∼-325bp region to be expected as UL47 promoter part into the indicator plasmid which includes the β-glucuronidase gene. In the transient promoter activation experiment, the promoter activity was increased up to 48-fold in the HCMV(AD169 strain) infected cells. This result indicates that the viral infection is necessary for the UL47 promoter activation occurred in only late times. In order to express UL47 protein in Escherichia coli, we have cloned the N-terminal part(+1∼+818) of UL47 open reading frame(ORF) into pET14b vector. After transformed the cloned DNA(pET14b-UL47N) into BL21(DE3)pLysS, we identified the UL47 protein was expressed by IPTG(isopropyl β-D-thiogalactopyranoside) induction.

록인 증폭기에 의한 L(C,R) 회로 공진점 결정의 한 방법

정세민,홍진태,최규황,노지현 東亞大學校 附設 基礎科學硏究所 1994 基礎科學硏究論文集 Vol.11 No.1

L(C,R)회로의 공진점을 결정하기 위해 록인 증폭기에 의한 sequential spectrum analysis(SSA)법을 적용해 보았다. 시료는 변압기였으며, 단일 소인방식과 왕복 소인방식을 비교해 보았다. 또한 실험 결과의 신뢰도를 확인해보기 위해 실험결과를 변압기의 제작사 자료와 Q 미터(HP,4277A USA)에 의한 측정 결과와 비교하였다. 제작사 자료에 의하면 시료의 공진점은 두 권선의 각각은 5kHz(10kHz/2)였으나, 왕복소인에 의한 측정에서 시료의 공진점은 5.1kHz, 실험 결과로부터 계산된 Q 값은 4.3으로 주어졌고, 단일 소인법에 의해서는 공진점이 5.78KHz로 주어졌으며, Q 미터에 의한 Q 값은 4.9(9.8/2)였다. 실험 결과들과 기준 Q 미터에 의한 측정결과를 비교해본 결과, 양방향 소인법이 단일 방향소인법 보다 더 신뢰할 수 있음을 알 수 있었으며, SSA법에 의한 측정이 기존의 상용 전문기기에 의한 측정에 못지 않음과 전문기기의 측정 하한 문제를 극복하는 좋은 대안이 될 수 있음을 알 수 있었다. An accurate method named the sequential spectrum analysis(SSA) using a lock-in amplifier(LIA) of the heterodyne type was investigated to determine the resonance frequency of a LC(C,R) circuit : A heavy winding transformer was used as an inductor in the circuit. In this experiment, two types of the sweep method-one way and round trip method were examined. The resonance frequency of each method were compared with the manufacturer's data and those by the reference Q meter(HP,4277A, USA)for the experimental confidence: Measured resonance frequency by the round-trip method was 5.1 kHz, Q value at this frequency was about 4.3, while those by the single-sweep method were given by 5.78 kHz for the resonance frequency, 2.3 for the Q. On the other hand, the resonance frequency of manufacturer's data was 5 kHz and Q value by the reference Q meter was 4.9(9.8/2)at that frequency. From the comparison, it might be concluded that the SSA by the round-trip method was more reliable and accurate than the one-way method and relatively inexpensive, good alternative with respect to the professional instrument.

金銀花 및 魚腥草가 人體 癌細胞柱에 미치는 影響

陳千植,崔政和,鄭鉉雨 대한동의병리학회 1996 동의생리병리학회지 Vol.10 No.1

국내 사망률 가장 높은 비율을 차지하고 있는 惡性腫瘍에 대하여 국내외적으로 다양하게 연구되고 있다. 그러나 아직까지도 腫瘍을 치료하기 위하여 手術治療·放射線治療·免疫治療·化學治療등의 많은 치료법을 개발하고, 또한 실험실 및 임상에서 많은 연구보고가 이루어지고 있지만 腫瘍의 치료원칙 및 치료약물에 대해서 미흡한 것이 국내의 현실이다. 일반적으로 抗癌劑를 이용한 化學治療등이 사용되고 있지만 이에 따른 副作用이 많아 본 연구자들은 抗癌劑와 韓藥材를 倂用投與하여 副作用을 최소화하고, 이에 따라 韓藥材가 正常細胞에 어떠한 영향을 미치고 , 癌腫細胞에는 어떠한 영향을 미치는가에 대하여 관찰한 결과 有意性이 있어 보고하게 되었다. 淸熱作用이 金銀花와 魚腥草를 인체의 皮膚癌細胞인 A431 細胞, 子宮癌細胞인 HeLa 細胞, 急性白血病細胞인 MOLT-4 細胞, 慢性骨髓性白血病細胞인 K562 細胞에 대한 細胞毒性과 抗癌劑인 mitomycin C와 倂用처리결과를 MTT assay를 통하여 관찰하였다. 또한 正常細胞에 대한 細胞毒性을 검색하기 위하여 마우스 섬유아세포(Balb/c3T3), 마우스 胸線 및 腥臟細胞, human lymphocyte에 미치는 細胞毒性을 검토하였다. 魚腥草는 慢性骨髓性白血病細胞柱에 직접적인 細胞毒性을 나타냈고, mitomycin C와 倂用 處理時 細胞毒性을 증가시켰으며, 마우스 섬유아細胞인 Balb/c 3T3 細胞의 增殖을 억제시켰다. 또한 金銀花는 마우스 胸線細胞의 增殖을 촉진시켰고, human lymphocyte의 增殖을 촉진시켰으며, 金銀花 및 魚腥草 모두는 마우스 腥臟細胞의 增殖을 촉진시켰다. The purpose of this research was to investigate effect of water extract of Flos Lonicerae and Herba Houttuyniae on the proliferation of human cancer cell-lines. The effects of Flos Lonicerae and Herba Houttyniae on the proliferation of A431, HeLa, MOLT-4, K562 cells, Balb/c 3T3 cells, mouse thymocytes, splenocytes and human lymphocytes were estimated by MTT colorimetric assay. 1. Flos Lonicerae and Herba Houttuyniae inhibited the proliferation of K562 cells. 2. The cytotoxicity of mitomycin C on K562 cells was decreased by the combination of Herba Houttuyniae. 3. Herba Houttuyniae inhibited the proliferation of Balb/c 3T3 cells. 4. Flos Lonicerae stimulated the proliferation of thymocytes. 5. Flos Lonicerae and Herba Houttuyniae stimulated the proliferation of splenocytes. 6. Flos Lonicerae stimulated the proliferation of human lymphocytes.

[논문]연약지반 성토시의 면적비를 활용한 안정관리기법

임종철,엄진현,박이근 釜山大學校生産技術硏究所 2003 生産技術硏究所論文集 Vol.62 No.-

국제 무역시대의 도래로 항만 물동량이 크게 증가함에 따라 항만시설의 확충이 시급한 실정이다. 비교적 양호한 지반에서의 항만공사는 종래에 대부분 행해졌으며, 현재는 아주 연약한 지반에서의 해안매립을 통한 부지조성 등의 대규모 공사가 진행중이다. 연약지반 성토시 하부지반은 압밀침하와 전단변형이 동시에 발생하므로, 양자를 고려한 안정성 판단이 매우 중요하다. 본 연구에서는 현장에서 파괴가 발생한 부산 신항만공사의 서방파제 계측결과를 분석하고 수치해석을 수행하여, 면적비 안정관리기법을 제안하였다.