SOD2와 NDPK2 유전자 집적에 의한 페튜니아의 아황산가스 저항성 증진

이수영(Su Young Lee),천경성(Kyeong-Seong Cheon),김소영(So Young Kim),권오현(O Hyeon Kwon),이혜진(Hye Jin Lee),김원희(Won Hee Kim),유봉식(Bong Sik Yoo) 한국원예학회 2016 원예과학기술지 Vol.34 No.1

유전자 집적에 의해 아황산가스 저항성 증진 페튜니아를 개발하고자 비생물적스트레스 저항성 유전자로 널리 알려진 SOD2 와 NDPK2유전자가 각각 도입된 SOD2 형질전환 계통 SOD2-2-1-1-35(T₄)[S(T₄)]와 NDPK2 형질전환 계통 NDPK2-7-1(T₂)[N7-1(T₂)]간 상호교잡을 실시하여 교잡 제1세대를 획득하였다. 교잡 제1세대 중 SOD2와 NDPK2유전자가 모두 집적된 개체 획득율은 32.1-73.0%이었고, SOD2와 NDPK2유전자가 모두 집적된 개체는 SOD2 또는 NDPK2유전자가 단독 도입된 개체에 비해 아황산가스 30ppm처리 피해율이 2.6-5.1배 낮아 아황산가스 저항성이 증진되었음을 확인하였다. 또한 아황산가스 저항성이 증진된 교잡 제1세대를 자가수분에 의해 세대진전 시켜 획득한 교잡 제2세대의 아황산가스 저항성 검정을 통하여 교잡 제1세대에서의 아황산가스 저항성이 후대에서도 안정적으로 발현함을 확인하였다. 또한, 광합성 효율 증진 조사를 통해 아황산가스 저항성이 증진된 SOD2와 NDPK2유전자 집적 후대가 아황산 가스에 대한 스트레스를 덜 받고 있음을 확인하였고, RT-qPCR분석을 통해 SOD2와 NDPK2유전자 집적 후대의 아황산가스 저항성 증진이 SOD2와 NDPK2 유전자 집적에 의한 효과임을 확인하였다. The aim of this study was to develop a transgenic petunia with enhanced resistance to sulfur dioxide (SO₂) gas by stacking two genes, SOD2 and NDPK2, which are both known to confer resistance to abiotic stresses. The first-generation hybrids (TF1) were obtained through reciprocal crosses between an SOD2-transgenic line SOD2-2-1-1-35(T₄)[S(T₄)] and an NDPK2- transgenic line NDPK2-7-1(T₂)[N7-1(T₂)]. Approximately 32.1-73.0% of the first-generation hybrids (TF₁) carried both SOD2 and NDPK2 genes. These hybrids showed 2.6 and 5.1 times less damage than hybrids carrying only SOD2 or NDPK2 genes, respectively, when they were treated with SO₂ gas at 30 ppm. This confirmed that the heterozygous hybrids were more resistant to SO₂ than the hybrids carrying either one of the resistance genes. Second-generation hybrids (TF₂) were obtained by selfing the TF₁ individuals. We confirmed the expression of the stacked genes in the TF₂ hybrids by phenotypic observation of their response to SO₂ gas at 30 ppm as well as using RT-qPCR and photosynthetic efficiency.

The Effects of Participation in Physical Activity Programs Based on Self-Determination on Developmentally Disabled Children`s Gross Motor Skills, Concentration, and Communicative Competence

( So Hyung Kim ),( Hye Min Kim ),( Hyun Su Lee ) 한국체육학회 2015 국제스포츠과학 학술대회 Vol.2015 No.1

Purpose: The purpose of this study is to provide children with developmental disorders with physical activities that are preferred based on self-determination theory and verifying the effects. This study is a follow-up study of one conducted by So Hyung Kim, Hye-Min Kim, and Hyun-Su Lee(2015) and it chose a type among preference types classified through Q methodologies. Afterwards, programs were developed based on physical activities preferred by children who fit that type, and then applied. Method: The study selected 28 children meeting the criteria through purposive sampling from six- to twelve-year-old disabled children living in C City and attending a kindergarten, special- education school, or general school`s special class. The subjects were made to classify 24 adapted physical activity cards developed for this research, and their preferred types were analyzed with QUANL PC Program. The type (Type 1) that had the most subjects in Type Q conducted the adapted physical activity they preferred twice a week, for 50 minutes in each session, for total 15 weeks. To examine the effectiveness of the program, the ATA concentration test, frostig-MSTB, percentage of correct consonants test, and maximal phonation time test were performed. Lastly, to analyze data for verifying hypotheses, SPSS for Window 10.0 was employed for repeated measured two-way ANOVA. Result: First, developmentally disabled children participating in the adapted physical activity program based on their self-determination showed more change in bending forward, standing long jump, shuttle run walking on a balance beam, balancing on one leg than does not in gross motor skills. Second, developmentally disabled children participating in the adapted physical activity program based on their self-determination showed more change in omission error than those not in concentration skills. Third, developmentally disabled children participating in the adapted physical activity program based on their self-determination did not show more change in the percentage of correct consonants (%) and in maximal phonation time (MPT) which is one of the aerodynamic outcomes than those not in communicative competence. Conclusion: Research results allowed recognition of the academic importance of the self-determination of disabled children through program applications made by the decisions of the disabled children themselves. Efforts to develop self-determination enhancement programs for disabled children in physical training fields will also be needed.

Perspectives of Integrative Cancer Genomics in Next Generation Sequencing Era

Kwon, So-Mee,Cho, Hyun-Woo,Choi, Ji-Hye,Jee, Byul-A,Jo, Yun-A,Woo, Hyun-Goo Korea Genome Organization 2012 Genomics & informatics Vol.10 No.2

The explosive development of genomics technologies including microarrays and next generation sequencing (NGS) has provided comprehensive maps of cancer genomes, including the expression of mRNAs and microRNAs, DNA copy numbers, sequence variations, and epigenetic changes. These genome-wide profiles of the genetic aberrations could reveal the candidates for diagnostic and/or prognostic biomarkers as well as mechanistic insights into tumor development and progression. Recent efforts to establish the huge cancer genome compendium and integrative omics analyses, so-called "integromics", have extended our understanding on the cancer genome, showing its daunting complexity and heterogeneity. However, the challenges of the structured integration, sharing, and interpretation of the big omics data still remain to be resolved. Here, we review several issues raised in cancer omics data analysis, including NGS, focusing particularly on the study design and analysis strategies. This might be helpful to understand the current trends and strategies of the rapidly evolving cancer genomics research.

남자대학생의 흡연태도와 행동에 관한 연구

강경진,김정미,민혜영,안소진,오경숙,은경희,이현,이희선,홍주미,김수지 이화여자대학교 간호과학대학 2003 이화간호학회지 Vol.- No.37

There have been so many researches about problems of cigarettes and relation of smoking and heath since 1950's and generally known that smoking is one of the worst factor of increasing disease and mortality. No matter what such many proofs about various problems of smoking have been researched, smoking rate of current male collage students has not been decreased. Besides, most of the researches about cigarette use and its addiction have been focused on the adolescent or adult women smokers or adult male, not male collage student. Therefore, the subject of this study is about smoking attitude and smoking behavior of male collage students by general features. Smoking attitude was analyzed by modifying 'Guidelines for the content of tobacco smoking surveys of the general population'(WHO, 1982). Two hundred of male collage students participated to the distributed questionnaire for one week in May. The smoking attitude was categorized into duration of smoking and number of cigarette per day(CPD), and nicotine dependency, investigated by Fagerstrom Tolerance Questionnaire(FTQ) (Fagerstorm & Schneider, 1989). All statistics were performed by procedure of SAS according to number, percentage, mean, standard deviation, and ANOVA. The results are as follows: 1. Smoking attitude level was mean 25.73±5.15 marks, ranging from 13 to 39 marks of the total 40 marks. 2. Duration of cigarettes, number of cigarette per day, and nicotine dependency were mean 5.14±2.84 years, 12.88±6.85 pieces, and 4.78±1.69, respectively. 3. Smoking attitude showed significance according to general features such as marital state and allowance, but the significance according to marital state is not valuable because the numerical difference is so big between married one and single that they cannot be compared with each other. 4. Duration of smoking was significant according to general features such as age and allowance. 5. Smoking amount showed significance the factors of age only. 6. ll1ere was no significance in nicotine dependency according to general features. As a result, smoking attitude of male collage student in this study shows moderate level. Smoking attitude and duration is significant according to allowance, and smoking amount is significant according to age. Nicotine dependency according to general features is not significant but comparing with the preceding study about adult male, male collage students in this study are respectively more nicotine-dependent than adult male smokers. In conclusion, the right allowance usage strategies and effective heath education focused on the young are needed. In addition, helpful programs and information spreading strategies are necessary for establishing higher level of smoking attitude. At last, systemic regulations in daily living for ""anti-nico-dependent"" and perspective reforming of male university student are needed for reducing nicotine dependency.

해안선을 따라 이동하는 켈빈파의 회전수조 모형 실험

蘇鮮變,金明煥,許相旭,李惠眞,梁潤實 공주대학교 기초과학연구소 2000 自然科學硏究 Vol.9 No.-

켈빈파란 회전계의 북반구에서 육지를 오른쪽에 두고 해안선을 따라 이동하는 파이다. 이 켈빈파를 가시화하기 위해 밀도차를 갖는 두 층의 유체를 만들고 경계면에 생기는 내부캘빈파를 가시화하여 변화를 관찰하였다. 지금까지는 해안가에서 나타나는 밀물과 썰물을 단순히 달의 인력으로 인한 조석으로만 생각했었는데, 본 실험을 통하여 그것이 지구자전의 영향을 받아 회전하는 조석파임을 알게되었다. Kenvin wave moves along the shoreline from the Northern hemisphere of rotating system in right of the land. Yeu observe s veriety for the visibity of it, we make two layers to have divergence of density, then it is visible the interior of Kelvin wave in a boundary plane. You thought simply the ebb and flow of the tide that they was the tidal movement to be due to the gravitation of the moon on the shore. But we know that Kelvin wave is rotating tide wave affected by revolution of the earth.

미만성 신경섬유종 -11예의 임상 및 병리학적 분석-

박소영,이혜경,백세민 대한병리학회 1995 Journal of Pathology and Translational Medicine Vol.29 No.2

보육교사의 아동학대 인식과 신고의무에 대한 태도

김소연,윤혜미 충북대학교 교육 ·생활연구소 생활과학연구센터 2003 생활과학연구논총 Vol.7 No.1

The purpose of this study was to find out the perception of child abuse and attitudes toward reporting among educare center teachers who were specified as the mandatory reporters of child abuse in Child Welfare Law amended in 2000. The subjects of this study were 312 preschool teachers, 231 from urban and 81 from rural area, Self-administered social survey method was employed. The data were analyzed by frequency, percentage, mean(M), standard deviation(SD), t-test, Chi-Square, Person correlation using SPSSWIN program. The results are as follow ; (1) Educare center teachers were highly perceptive of child abuse and quite alert to the occurrence of child abuse. Preschool teachers in the rural area tended to experience higher number of child abuse occurrence than those who in the urban area. (2) Significantly low reporting rate was observed. While 29.8% of educare center teachers had experiences of child abuse observance, only two teachers among them had reported to the authority. The reasons appeared that the most of educare center teachers are not aware of the proper timing for reporting and they don't have the correct information concerning child abuse reporting. Only 26.9% of the subjects, answered that they would report the child abuse cases to the proper authority. It was shown that the child abuse reporting behavior was affected by what happenes after reporting and the extent of their perception of seriousness of the child abuse. It was found that they had not had any formal educational opportunity on the subject of child abuse and reporting, Most of the subjects indicated the need for frequent on-the-job training and workshops on the issue.

유아교육기관의 운영관리 및 유아기 프로그램 평가

이소은,하진희,류혜선,박영주,주은혜,이종순 충북대학교 교육생활연구소 2001 생활과학연구논총 Vol.4 No.-

This purposes of this study were to assess the state of current administration and program contents in early childhood institutes. Using APECP(Assessnt Profile for Early Childhood Programs), assesment data were gathered from 27 apprentices in child care centers and 6 apprentices in Kindergarten in Cheongju. Descriptive statistics and Mann-Whitney test were used to analyze data. The result were as follows : (1) The distribution of percentile rate in administration was from 55% to 78%, and the area of Program Development scored the lowest. The assessments for Kindergarten were higher than those of child care center in the areas of Physical Facility, Food Service, and Program Management. (2) As for the program contents, percentile rate was from 62% to 80%, and generally the assessments for Kindergarten were higher than those of child care center. It appears that the certification and assessment system for ealry childhood institutions should be supervised more strongly in order to improve the qulaity of those institutions.

말티즈견에서 발생한 승모판폐쇄부전 1례

전소분,박혜연,김선아,정금선,이영원,박성준,김덕환,송근호 충남대학교 수의과대학 동물의과학연구소 2004 動物醫科學硏究誌 Vol.12 No.-

A 10 years old, intact male, Maltese dog with clinical signs of labored respiration, exercise intolerance and increasing of coughing was referred to the Veterinary Medical Teaching Hospital of Chungnam National University. Physical examination revealed tachycardia, panting and mild cyanosis. This case was diagnosed as mitral valve insufficiency by clinical signs, ascultation, blood chemistry, radiography and electrocardiography. This dog was treated with ACE inhibitor (enalapril), diuretics (furosemide) and bronchodilator (aminophylline) for 14 days. After treatment, clnical signs were disappeared and also this dog was needed to be continuous monitoring and sodium restricted diet.

청주시 소비자의 그린마케팅에 대한 인식과 그린제품 구매의도

권소영, 박혜영, 최혜미, 최현미, 유현정 충북대학교 생활과학연구소 2013 생활과학연구논총 Vol.17 No.2

The purpose of this study was to analyze consumers' perception of green marketing and purchase intention of green products. Questionnaire survey was conducted from 9 August to 29 August 2010. We have surveyed 298 consumers who were living in Cheongju area. Major findings are as follows. First, consumers' environment perception and involvement, awareness of green marketing, evaluation for green products, purchase intention of green products were generally positive. It means that Korean consumers have a positive attitude and behavioral intention for green consumption. Second, consumers responded high perception level for necessity of green marketing and purchase intention of green products. On the other hand, consumers think that green product manufacturers need to more protect our environment. Consumers evaluated the quality of green products is good but its price is high. Third, insufficient information was the most common response of the items asked for the resistance of the whole process for the production of green products. For benefit part, high quality and symbolic value of green consumer were given answer highly. Fourth, there were differences among the consumer groups from environment perception, awareness of green marketing, evaluation for green products, and purchase intention of green products by consumers' characteristics. Old aged group were high involved on environment and student group showed relatively low environment perception and involvement of green consumption. Four million won or less income groups were relatively higher interest in green products and eco-conscious consumer groups have a high degree of purchasing intention of green products.