소셜 미디어 데이터 마이닝을 통한 라이프스타일 호텔 공간 선호 요인에 관한 연구 - 2021년 이후 성수기 중심으로 -

양윤실,김용성,송석재 한국공간디자인학회 2023 한국공간디자인학회논문집 Vol.18 No.1

(Background and Purpose) Instagram, one of the social media platforms, provides image-oriented information and has a greater impact than existing information media by using it directly or leaving it after experience or visit, and the hotel industry is also actively marketing using Instagram. The scope of lifestyle hotels is gradually expanding, but currently, analysis data on architectural spaces for lifestyle hotels in Korea are insufficient. Therefore, this study aims to analyze the characteristics of the lifestyle hotel space targeting millennials with strong personality using the image of a specific platform called Instagram and suggests it as basic data for future lifestyle hotel construction. (Method) To this end, the concept and spatial composition of lifestyle hotels are reviewed through previous studies, and the characteristics thereof are identified. A framework for analysis to analyze spatial components was established, and prior analysis studies using image data from Instagram were conducted. Image data mining was conducted using a neural network model, one of the classification techniques of data mining. Based on this, the space of lifestyle hotels that mainly appear in the image was identified, and spatial preference factors were derived for this. This is to provide basic data on future lifestyle hotel construction plans and present the direction of hotel construction. (Results) Therefore, this study analyzed users' preferred spaces through image mining based on the framework of analysis on the space composition of selected lifestyle hotels, and derived the results that images appeared the most in swimming pools, rooms, lobby lounges, restaurants, bars, elevators, and corridors. After analyzing the preference factors in the main space through image mining in the same space based on the image of the upper space, it was confirmed that the preferred space characteristics of Instagram users are the preference factors for each hotel, such as exhibition elements using colorful patterns or art works, high floor height, round stairs, huge columns, and various color lights. (Conclusions) This analyzed information on users' direct experiences or visits through a large amount of data through a big data analysis method called image data mining for existing hotel architectural spaces. Through data mining of the image shown on Instagram, it was possible to identify the preference factors for the hotel space preferred by users. Therefore, it can be seen that the composition of the space, architecture, interior, and visual elements through exhibition in the space of the lifestyle hotel play a very important role in the hotel space. This study identified the spatial composition of lifestyle hotels and their preference factors as a method of analyzing large amounts of big data through image data mining on Instagram, and it is expected to be used as basic data for lifestyle hotel building plan research. (연구배경 및 목적) 소셜 미디어 플랫폼 중 하나인 인스타그램(Instagram)은 이미지 중심의 정보를 제공하여 직접 사용하거나 체험 또는 방문 후에 남기는 게시물을 통한 정보로 기존 정보 매체보다 큰 영향을 주고 있으며, 호텔 업계에서도 인스타그램을 활용한 마케팅을 적극적으로 펼치고 있다. 라이프스타일 호텔의 범위는 점차 확대되고 있으나, 현재 국내의 라이프스타일 호텔에 대한 건축 공간에 관한 분석 자료는 미비한 실정이다. 이에 본 연구는 개성이 강한 밀레니얼 세대를 타겟으로 한 라이프스타일 호텔 공간을 인스타그램이라는 특정 플랫폼의 이미지를 이용하여 이미지에서 주요하게 나타나는 공간에 대한 특성을 분석하고자 하는 것으로 향후 라이프스타일 호텔 건축에 대한 기초자료로 제안하려고 한다. (연구방법) 이를 위해 선행연구를 통한라이프스타일 호텔의 개념과 공간 구성을 고찰하고 그에 대한 특징을 파악한다. 공간 구성 요소를 분석하기 위한 분석의 틀을설정하고 인스타그램의 이미지 데이터를 활용한 분석 선행 연구를 진행하였다. 데이터 마이닝의 분류 기법 중 하나인 신경망 모델을 활용하여 이미지 데이터 마이닝을 진행하였다. 이를 바탕으로 이미지에서 주로 나타나는 라이프스타일 호텔의 공간을 파악하였으며 그에 대한 공간 선호 요인을 도출하였다. 이는 향후 라이프스타일 호텔 건축 계획에 관한 기초자료로 제공하고자 한다. (결과) 이에 본 연구에서는 선정된 라이프스타일 호텔의 공간 구성에 대한 분석의 틀을 기준으로 이미지 마이닝을 통한 이용자들의 선호 공간을 분석한 결과 수영장과, 객실, 로비 라운지, 레스토랑 ‧ 바, 엘리베이터, 복도 공간에서 이미지가 가장 많이나타났다는 결과를 도출하였다. 이후 상위 공간의 이미지를 기준으로 동일 공간 내 세부 분류 이미지마이닝을 통해 주요 공간에서 나타나는 선호 요인을 분석한 결과 인스타그램 이용자들의 이미지에서 배경이 되는 선호 공간 특성은 화려한 패턴이나 미술작품 등을 활용한 전시 요소, 개방감을 주는 높은 층고, 원형 계단, 거대한 기둥, 다양한 색상 조명 등 호텔별로 가지는 특정한요소가 인스타그램 이미지에서 주로 나타나는 공간 선호 요인임을 확인하였다. (결론) 이는 기존의 호텔 건축 공간에 대해 이미지 데이터 마이닝이라는 빅 데이터 분석 방법을 통해 이용객들의 직접적인 체험이나 방문에 대한 정보를 대량의 데이터를 통해분석하였다. 인스타그램에서 나타난 이미지의 데이터 마이닝을 통해 이용자들의 호텔 공간에 대한 선호 요인을 파악할 수 있었다. 따라서 라이프스타일 호텔의 공간에서 공간의 구성 여부나 건축 및 인테리어, 전시를 통한 시각적 요소는 호텔 공간에서 매우 중요한 역할을 한다는 것을 알 수 있다. 본 연구는 인스타그램의 이미지 데이터 마이닝을 통한 대량의 빅 데이터 분석 방법으로 라이프스타일 호텔의 공간구성과 그에 대한 선호 요인을 파악하였으며 라이프스타일 호텔 건축 계획 연구의 기초자료로 활용되기를 기대한다.

Chronic Ca2+ influx through voltage-dependent Ca2+ channels enhance delayed rectifier K+ currents via activating Src family tyrosine kinase in rat hippocampal neurons

양윤실,전상찬,김동관,은수용,정성철 대한약리학회 2017 The Korean Journal of Physiology & Pharmacology Vol.21 No.2

Excessive influx and the subsequent rapid cytosolic elevation of Ca2+ in neurons is the major cause to induce hyperexcitability and irreversible cell damage although it is an essential ion for cellular signalings. Therefore, most neurons exhibit several cellular mechanisms to homeostatically regulate cytosolic Ca2+ level in normal as well as pathological conditions. Delayed rectifier K+ channels (IDR channels) play a role to suppress membrane excitability by inducing K+ outflow in various conditions, indicating their potential role in preventing pathogenic conditions and cell damage under Ca2+-mediated excitotoxic conditions. In the present study, we electrophysiologically evaluated the response of IDR channels to hyperexcitable conditions induced by high Ca2+ pretreatment (3.6 mM, for 24 hours) in cultured hippocampal neurons. In results, high Ca2+-treatment significantly increased the amplitude of IDR without changes of gating kinetics. Nimodipine but not APV blocked Ca2+-induced IDR enhancement, confirming that the change of IDR might be targeted by Ca2+ influx through voltage-dependent Ca2+ channels (VDCCs) rather than NMDA receptors (NMDARs). The VDCC-mediated IDR enhancement was not affected by either Ca2+-induced Ca2+ release (CICR) or small conductance Ca2+-activated K+ channels (SK channels). Furthermore, PP2 but not H89 completely abolished IDR enhancement under high Ca2+ condition, indicating that the activation of Src family tyrosine kinases (SFKs) is required for Ca2+-mediated IDR enhancement. Thus, SFKs may be sensitive to excessive Ca2+ influx through VDCCs and enhance IDR to activate a neuroprotective mechanism against Ca2+-mediated hyperexcitability in neurons.

The venom of jellyfish, Chrysaora pacifica, induces neurotoxicity via activating Ca2+-mediated ROS signaling in HT-22 cells

양윤실,강영준,김혜지,김민수,정성철 한국응용생명화학회 2019 Journal of Applied Biological Chemistry (J. Appl. Vol.62 No.4

Stings of jellyfish, which frequently occur in a warm season, cause severe pain, inflammation and sometimes irreversible results such as the death. Harmful venoms from jellyfish, therefore, have been studied for finding the therapeutic agents to relieve pain or to neutralize toxic components. However, it is still unclear if and how jellyfish venom reveal neuronal toxicity even though pain induction seems to result from the activation of nociceptors such as nerve endings. In this study, using HT-22 cell line, we investigated neurotoxic effects of the venom of Chrysaora pacifica (CpV) which appears in South-East ocean of Korea. In 3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, CpV significantly reduced the viability of HT-22 cells in a dosedependent manner. Additionally, in 2',7'-Dichlorofluorescin diacetate fluorescence test under the culture condition lacking dominant inflammatory factors, CpV remarkably increased the production of intracellular reactive oxygen species (ROS). Reduced responsive fluorescence to Rhodamine123 and increased expression of intracellular cytochrome c were also observed in HT-22 cells treated with CpV. These indicate that CpV-reduced viability of HT-22 cells may be due to the activation of apoptotic signalings mediated with oxidative stress and mitochondrial dysfunction. Furthermore, removing Ca2+ ion or adding N-acetyl-Lcystein remarkably blocked the CpV effect to reduce the viability of HT- 22 cells. The findings in this study clearly demonstrate that CpV may activate Ca2+-mediated ROS signalings and mitochondrial dysfunction resulting in neuronal damage or death, and suggest that blocking Ca2+ pathway is a therapeutic approach to possibly block toxic effects of jellyfish venoms.

일본 그룹 홈의 건축적 특징에 관한 기초연구

양윤실,김태일 대한건축학회지회연합회 2013 대한건축학회지회연합회 학술발표대회논문집 Vol.2013 No.1

본 연구는 일본 그룹홈 협회 명단을 기초로 하여 설문조사와 아울러 시설도면 요청을 통해 총 103개소의 기초적인 자료 를 수집하여 그룹 홈의 공간분석을 하였다. 그룹 홈의 규모는 대부분 1∼2층 소규모이며, 유니트 수는 대략적으로 1개 혹은 2개로 구성되고 유니트별 거실 수는 9개로 구성되어 1실 1거주의 독립된 생활을 유지하는 것으로 나타났다. 배치형태는 ㄷ 자형이 많았으며, 대부분의 시설들이 지역사회와의 교류프로그램을 갖고 있는 것으로 분석되었다. 본 연구결과는 지속적으로 증가하는 치매노인을 위한 시설을 위한 기준 설정에 기초자료로 활용될 수 있으며, 한국인의 정서에 맞는 적합한 배치, 동 선, 유니트 형태 등 다양한 공간구성에 대한 추가적 연구가 필요하다고 본다.

일본 그룹 홈의 건축적 특징에 관한 기초연구

양윤실(Yang Yoon-Sil),김태일(Kim Tae-Il) 한국실내디자인학회 2014 한국실내디자인학회논문집 Vol.23 No.4

According to data from the national office of Statistics Korea and Ministry of Health and Welfare, as the elderly population increases, the dementia elderly population continues to increase and its future population growth rate is expected to be even faster. In particular, the Dementia Management Act has been in effect since February 2012, and active efforts has been made for a policy for the dementia management. The purpose of this study is to establish standards on building plans based on the appropriate scale and spatial configuration on facilities planning for the elderly with dementia. Specifically, the basic data were collected with a request for a total of 103 points on the basis of a database of group homes in the survey managed by the Japan Association of Group Homes. Specific information of the research includes the management body of facilities operation, scale of the facilities, number of units and configuration of personal living space, and the collected survey data and drawings were statistically proceed and analyzed using the SPSS WIN 20.0. analysis results are summarized as follows. first, most of the group homes come to the small size of the 1-2 story home ; the approximate number of units is one or two per home, and each unit consists of nine rooms. second, a number of group homes with the building area of 300m² have the U-shaped arrangement which is advantageous in the extension and facilities maintenance. In conclusion, this study is to be the fundamental data for judgments that can be used to establish standards for the facilities for the dementia elderly whose population continues to increase. In addition, further study is necessary to establish suitable design conditions of our country.

The Downregulation of Somatic A-Type K+ Channels Requires the Activation of Synaptic NMDA Receptors in Young Hippocampal Neurons of Rats

강문석,양윤실,김선희,박주민,은수용,정성철 대한약리학회 2014 The Korean Journal of Physiology & Pharmacology Vol.18 No.2

The downregulation of A-type K+ channels (IA channels) accompanying enhanced somatic excitabilitycan mediate epileptogenic conditions in mammalian central nervous system. As IA channels are dominantlytargeted by dendritic and postsynaptic processings during synaptic plasticity, it is presumablethat they may act as cellular linkers between synaptic responses and somatic processings under variousexcitable conditions. In the present study, we electrophysiologically tested if the downregulation ofsomatic IA channels was sensitive to synaptic activities in young hippocampal neurons. In primarilycultured hippocampal neurons (DIV 6~9), the peak of IA recorded by a whole-cell patch was significantlyreduced by high KCl or exogenous glutamate treatment to enhance synaptic activities. However, thepretreatment of MK801 to block synaptic NMDA receptors abolished the glutamate-induced reductionof the IA peak, indicating the necessity of synaptic activation for the reduction of somatic IA. Thiswas again confirmed by glycine treatment, showing a significant reduction of the somatic IA peak. Additionally, the gating property of IA channels was also sensitive to the activation of synaptic NMDAreceptors, showing the hyperpolarizing shift in inactivation kinetics. These results suggest thatsynaptic LTP possibly potentiates somatic excitability via downregulating IA channels in expressionand gating kinetics. The consequential changes of somatic excitability following the activity-dependentmodulation of synaptic responses may be a series of processings for neuronal functions to determineoutputs in memory mechanisms or pathogenic conditions.

Dieckol Attenuates Microglia-mediated Neuronal Cell Death via ERK, Akt and NADPH Oxidase-mediated Pathways

추연지,박지윤,우진지,이지형,양윤실,강문석,정성철,박주민,유은숙,김성호,안상미,석경호,은수용 대한약리학회 2015 The Korean Journal of Physiology & Pharmacology Vol.19 No.3

Excessive microglial activation and subsequent neuroinflammation lead to synaptic loss and dysfunction as well as neuronal cell death, which are involved in the pathogenesis and progression of several neurodegenerative diseases. Thus, the regulation of microglial activation has been evaluated as effective therapeutic strategies. Although dieckol (DEK), one of the phlorotannins isolated from marine brown alga Ecklonia cava, has been previously reported to inhibit microglial activation, the molecular mechanism is still unclear. Therefore, we investigated here molecular mechanism of DEK via extracellular signal-regulated kinase (ERK), Akt and nicotinamide adenine dinuclelotide phosphate (NADPH) oxidase-- mediated pathways. In addition, the neuroprotective mechanism of DEK was investigated in microglia-- mediated neurotoxicity models such as neuron-microglia co-culture and microglial conditioned media system. Our results demonstrated that treatment of anti-oxidant DEK potently suppressed phosphorylation of ERK in lipopolysaccharide (LPS, 1 μg/ml)-stimulated BV-2 microglia. In addition, DEK markedly attenuated Akt phosphorylation and increased expression of gp91phox, which is the catalytic component of NADPH oxidase complex responsible for microglial reactive oxygen species (ROS) generation. Finally, DEK significantly attenuated neuronal cell death that is induced by treatment of microglial conditioned media containing neurotoxic secretary molecules. These neuroprotective effects of DEK were also confirmed in a neuron-microglia co-culture system using enhanced green fluorescent protein (EGFP)-- transfected B35 neuroblastoma cell line. Taken together, these results suggest that DEK suppresses excessive microglial activation and microglia-mediated neuronal cell death via downregulation of ERK, Akt and NADPH oxidase-mediated pathways.

해안선을 따라 이동하는 켈빈파의 회전수조 모형 실험

蘇鮮變,金明煥,許相旭,李惠眞,梁潤實 공주대학교 기초과학연구소 2000 自然科學硏究 Vol.9 No.-

켈빈파란 회전계의 북반구에서 육지를 오른쪽에 두고 해안선을 따라 이동하는 파이다. 이 켈빈파를 가시화하기 위해 밀도차를 갖는 두 층의 유체를 만들고 경계면에 생기는 내부캘빈파를 가시화하여 변화를 관찰하였다. 지금까지는 해안가에서 나타나는 밀물과 썰물을 단순히 달의 인력으로 인한 조석으로만 생각했었는데, 본 실험을 통하여 그것이 지구자전의 영향을 받아 회전하는 조석파임을 알게되었다. Kenvin wave moves along the shoreline from the Northern hemisphere of rotating system in right of the land. Yeu observe s veriety for the visibity of it, we make two layers to have divergence of density, then it is visible the interior of Kelvin wave in a boundary plane. You thought simply the ebb and flow of the tide that they was the tidal movement to be due to the gravitation of the moon on the shore. But we know that Kelvin wave is rotating tide wave affected by revolution of the earth.

미토콘드리아 기능을 통해 내인성 글루탐산이 신경세포 생존에 미치는 영향

노진우,김혜지,은수용,강문석,정성철,양윤실,Noh, Jin-Woo,Kim, Hye-Ji,Eun, Su-Yong,Kang, Moon-Suk,Jung, Sung-Cherl,Yang, Yoon-Sil 제주대학교 의과학연구소 2018 The Journal of Medicine and Life Science Vol.15 No.2

Neuronal excitotoxicity induces mitochondrial dysfunction and the release of proapoptotic proteins. Excitotoxicity, the process by which the overactivation of excitatory neurotransmitter receptors leads to neuronal cell death. Neuronal death by excitotoxicity was related to neuronal degenerative disorders and hypoxia, results from excessive exposure to excitatory neurotransmitters, such as glutamate. Glutamate acts at NMDA receptors in cultured neurons to increase the intracellular free calcium concentration. Therefore endogenous glutamate may be a key factor to regulate neuronal cell death via activating $Ca^{2+}$ signaling. For this issue, we tested some conditions to alter intracellular $Ca^{2+}$ level in dissociated hippocampal neurons of rats. Cultured hippocampal neuron were treated by KCl (20 mM), $CaCl_2$ (3.8 mM) and glutamate ($5{\mu}M$) for 24 hrs. Interestingly, The Optical Density of hippocampal neurons was increased by high KCl application in MTT assay data. This enhanced response by high KCl was dependent on synaptic $Ca^{2+}$ influx but not on intracellular $Ca^{2+}$ level. However, the number of neurons seemed to be not changed in Hoechst 33342 staining data. These results suggest that enhancement of synaptic activity plays a key role to increase mitochondrial signaling in hippocampal neurons.

Methyl lucidone exhibits neuroprotective effects on glutamate‑induced oxidative stress in HT‑22 cells via Nrf‑2/HO‑1 signaling

박지연,Khulan Amarsanaa,추연지,이지형,우진지,양윤실,은수용,정성철 한국응용생명화학회 2019 Applied Biological Chemistry (Appl Biol Chem) Vol.62 No.6

Oxidative stress causes neuronal cell death in various neurodegenerative diseases, such as Alzheimer’s disease, ischemia, and Parkinson’s disease. Therefore, reducing intracellular reactive oxygen species (ROS) has been evaluated as an effective treatment strategy for neurodegenerative disorders. Methyl lucidone (MLC) extracted from Lindera erythrocarpa Makino (Lauraceae) has been previously reported to exhibit microglial-mediated neuroprotective effects via inhibiting neuroinflammation. However, the antioxidant effects of MLC are still unclear. The aim of this study was to determine the neuroprotective mechanism of MLC in HT-22 neurons against oxidative stress induced by glutamate. In results, the pretreatment of MLC significantly enhanced the viability of HT-22 cells under glutamate-induced oxidative conditions, suggesting that MLC has a neuronal mechanism to protect neurons without microglial regulation. Also, the glutamate effect to increase ROS production was effectively blocked by MLC without any free radical scavenging activity. To induce this antioxidant effect, MLC upregulated the expression of heme oxygenase 1 (HO-1) and nuclear translocation of nuclear factor-E2-related factor 2 (Nrf-2), known as an intracellular antioxidant enzyme, and its transcription factor. Additionally, Akt phosphorylation regulating Nrf-2 was confirmed to be involved in the neuroprotective signaling activated by MLC. These results indicate that MLC may play a role as an antioxidant agent to inhibit neurodegenerative processes via activating antioxidant signaling pathways that include Nrf-2 and phosphatidylinositol 3-kinase (PI3K).