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The complete mitochondrial genome of a threatened loach (Sinibotia reevesae) and its phylogeny
Yuan‑Chao Zou,Bi‑Wen Xie,Chuan‑Jie Qin,Yong‑Ming Wang,Deng‑Yue Yuan,Rui Li,Zheng‑Yong Wen 한국유전학회 2017 Genes & Genomics Vol.39 No.7
In present study, the complete mitochondrial genome of Sinibotia reevesae was first sequenced using the next-generation sequencing technology and annotated using bioinformatic tools. The circular mitochondrial genome was 16,572 bp in length, and contained 13 proteincoding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and 1 displacement loop locus. It presents a typical gene organization and order for completely sequenced cypriniformes mitogenomes. The control region could be divided into three parts included the extended termination associated sequence domain, the central conserved domain and the conserved sequence block. Interestingly, two stemloop domains were found in control region and OL region, respectively. Furthermore, phylogenetic analyses using concatenated amino acid and nucleotide sequences of the 13 protein-coding genes with two different methods (Maximum likelihood and Bayesian analysis) both highly supported the close relationship of S. reevesae and Sinibotia superciliaris, which was in line with the previous classifications based on morphological and molecular studies. These data provide useful information for a better understanding of the mitogenomic diversities and evolution in fish as well as novel genetic markers for studying population genetics and species identification.
Deng’an Cai,Xiaopei Wang,Yaohui Shi,Xufeng Hao,Yuan Qian,Guangming Zhou 한국섬유공학회 2021 Fibers and polymers Vol.22 No.2
Transverse mechanical properties play an important role in the study of fiber reinforced composite materials. Anexperimental and numerical study on mechanical properties and damage behavior of unidirectional fiber reinforcedcomposites under transverse load is presented. The quasi-static tensile and compressive tests were carried out to obtainmechanical properties of unidirectional fiber reinforced composites under transverse tension and compression. The fracturemorphologies of the specimens were observed by scanning electron microscopy (SEM) to reveal their distinct damagemechanism. Considering the effects of interface, a new interfacial model is proposed. Two representative volume elements(RVEs) of basic and interfacial models are established for comparison analysis. The predicted results agree well with theexperimental data. The introduction of interfacial layer has little effect on the prediction of transverse modulus, but canimprove the accuracy of the predicted transverse strength. The fiber volume fraction has a great influence on the transversemechanical properties of unidirectional fiber reinforced composites. As the fiber volume fraction increases, its transversemodulus increases, but both the transverse strength and the ultimate strain decrease.
REPRESENTATIONS OF THE MOORE-PENROSE INVERSE OF 2×2 BLOCK OPERATOR VALUED MATRICES
Deng, Chun Yuan,Du, Hong Ke Korean Mathematical Society 2009 대한수학회지 Vol.46 No.6
We obtain necessary and sufficient conditions for $2{\tims}2$ block operator valued matrices to be Moore-Penrose (MP) invertible and give new representations of such MP inverses in terms of the individual blocks.
Deng, Yuan-fei(등원비),Park, Jihyun(박지현) 대한건축학회 2023 대한건축학회 학술발표대회 논문집 Vol.43 No.2
The proportion of single households in Korea has now risen dramatically to become the most common household type in the country. Because of the rapid progress of urbanization, the population density and building density of Seoul have risen dramatically, but because of this, many buildings in the city do not have a good indoor environment. The purpose of this study is to investigate the living comfort of studio rooms in which university students live in Seoul, to understand the strengths and weaknesses of their indoor environmental quality, and to provide guidance for improving their environmental quality. Because it is relatively good price and guarantees privacy, studio is the first choice for single-person households, especially for the university student population. Based on our assessment results, overall, university students currently living in studios in Seoul need more natural light, better ventilation, and quieter living environments than the status quo, which will require not only a reduction in building density and a widening of the distances between buildings in future development activities, but also more careful consideration of ventilation, heat and thermal insulation, and soundproofing in the design.
Phosphorylation of Silk Fibroin via Maillard Reaction and Its Behavior of Biomimetic Mineralization
Jingjing Yuan,Qian Zhou,Ping Wang,Chao Deng,Jiugang Yuan,Qiang Wang 한국섬유공학회 2019 Fibers and polymers Vol.20 No.8
Silk fibroin (SF) has the characteristics of non-toxicity, good biocompatibility and low immunogenicity, andexhibits many potential applications in biomaterial fields. In the present work, chemical phosphorylation of SF was carriedout via the Maillard reaction using D-glucose-6-phosphate (GP), aiming at increasing the number of binding-sites forcalcium ion and promoting the biomimetic mineralization of SF films. Changes in the molecular weight of SF before andafter GP grafting were analyzed by SDS-PAGE and SEC chromatogram. Structures, morphologies, and elementalcompositions for the fibroin-based films before and after mineralization were evaluated by the means of EDS, SEM, FTIR,and XRD. The results indicated that GP was successfully grafted onto SF surfaces, companying with a slight decrease in themolecular weight. Biomimetic mineralization led to a noticeable deposition of hydroxyapatite (HAp) on the film of SF-g-GP, and higher contents of calcium and phosphorous were detected on the mineralized material, revealing thatphosphorylation promoted the mineralization processing. Meanwhile, biocompatibility of the obtained SF-g-GP/HAp wasabove the acceptable level according to ISO 10993-5-2009. The present work provides an alternative to prepare the fibroinbasedbone repair materials.
Representations of the Moore-Penrose inverse of 2×2 block operator valued matrices
Chun Yuan Deng,Hong Ke Du 대한수학회 2009 대한수학회지 Vol.46 No.6
We obtain necessary and sufficient conditions for 2×2 block operator valued matrices to be Moore-Penrose (MP) invertible and give new representations of such MP inverses in terms of the individual blocks. We obtain necessary and sufficient conditions for 2×2 block operator valued matrices to be Moore-Penrose (MP) invertible and give new representations of such MP inverses in terms of the individual blocks.
Zhao, Yuan,Li, Yu,Deng, Chun,Yuan, Yi-Chao,He, Jin-Liang,Wang, Xi The Korean Institute of Electrical Engineers 2014 The Journal of International Council on Electrical Vol.4 No.3
The voltage waveform on the insulator by a direct lightning stroke is a short-tail lightning impulse. Experimental study and analysis of insulator breakdown characteristics under short-tail lightning impulses are given in this paper with the contrast of standard lightning impulse. The impacts of crossarm and transmission lines are also considered. The experimental results indicate that the insulator 50% breakdown voltage under short-tail impulse is significantly higher than that of standard wave with both positive and negative polarities, about 25~30%. The 50% breakdown voltage of insulator with negative-polarity short-tail impulse is about 5% higher than with positive-polarity one. The insulator voltage-time characteristics are also given with short-tail and standard lightning impulses.
Sijun Deng,Hui Yuan,Jine Yi,Yin Lu,Qiang Wei,Chengzhi Guo,Jing Wu,Zuping He,Li-yun Yuan 대한수의학회 2013 Journal of Veterinary Science Vol.14 No.3
To investigate the effects of gossypol acetic acid (GA) on proliferation and apoptosis of the macrophage cell line RAW264.7 and further understand the possible underlying mechanism responsible for GA-induced cell apoptosis,RAW264.7 cells were treated with GA (25∼35 μmol/L) for 24h and the cytotoxicity was determined by MTT assay, while apoptotic cells were identified by TUNEL assay, acridine orange/ethidium bromide staining and flow cytometry. Moreover, mitochondrial membrane potential (ΔΨ m) with Rhodamine 123 and reactive oxygen species (ROS) with DCFH-DA were analyzed by fluorescence spectrofluorometry. In addition, the expression of caspase-3 and caspase-9 was assessed by Western Blot assay. Finally, the GA-induced cell apoptosis was evaluated by flow cytometry in the present of caspase inhibitors Z-VAD-FMK and Ac-LEHD-FMK,respectively. GA significantly inhibited the proliferation of RAW264.7 cells in a dose-dependent manner, and caused obvious cell apoptosis and a loss of ΔΨ m in RAW264.7 cells. Moreover, the ROS production in cells was elevated, and the levels of activated caspase-3 and caspase-9 were up-regulated in a dose-dependent manner. Notably, GA-induced cell apoptosis was markedly inhibited by caspase inhibitors. These results suggest that GA-induced RAW264.7 cell apoptosis may be mediated via a caspase-dependent mitochondrial signaling pathway.