Phosphorylation of Silk Fibroin via Maillard Reaction and Its Behavior of Biomimetic Mineralization

Jingjing Yuan,Qian Zhou,Ping Wang,Chao Deng,Jiugang Yuan,Qiang Wang 한국섬유공학회 2019 Fibers and polymers Vol.20 No.8

Silk fibroin (SF) has the characteristics of non-toxicity, good biocompatibility and low immunogenicity, andexhibits many potential applications in biomaterial fields. In the present work, chemical phosphorylation of SF was carriedout via the Maillard reaction using D-glucose-6-phosphate (GP), aiming at increasing the number of binding-sites forcalcium ion and promoting the biomimetic mineralization of SF films. Changes in the molecular weight of SF before andafter GP grafting were analyzed by SDS-PAGE and SEC chromatogram. Structures, morphologies, and elementalcompositions for the fibroin-based films before and after mineralization were evaluated by the means of EDS, SEM, FTIR,and XRD. The results indicated that GP was successfully grafted onto SF surfaces, companying with a slight decrease in themolecular weight. Biomimetic mineralization led to a noticeable deposition of hydroxyapatite (HAp) on the film of SF-g-GP, and higher contents of calcium and phosphorous were detected on the mineralized material, revealing thatphosphorylation promoted the mineralization processing. Meanwhile, biocompatibility of the obtained SF-g-GP/HAp wasabove the acceptable level according to ISO 10993-5-2009. The present work provides an alternative to prepare the fibroinbasedbone repair materials.

Synthesis and Application of Oxidized Trehalose as a Hydrophilic Anti-crease Finishing Reagent for Cotton Fabric

Jiangfei Lou,Jiugang Yuan,Qiang Wang,Xuerong Fan 한국섬유공학회 2021 Fibers and polymers Vol.22 No.4

In order to improve the anti-crease property of cotton fabrics, it is necessary to chemically cross-link functionalgroups in the fabric via anti-crease finishing. However, the large amount of hydroxyl groups in cotton cellulose is consumedduring the cross-linking finishing process, which greatly reduces the hydrophilicity of the fabric, and thus, staining of cottonfabrics is greatly reduced. Consequently, the fabric becomes non-conducive to subsequent processing and is not well suitedfor typical applications of cotton fabric. In this paper, a new type of anti-crease finishing reagent for cotton fabric wasprepared by using sodium periodate to oxidize Trehalose. This new finishing improved the anti-crease property, and thehydrophilicity and staining of the fabric. The optimum preparation process of oxidized trehalose (OTr) was determined byanalyzing the influence of initial pH, molar ratio of trehalose to sodium periodate, and the reaction time on the amount ofaldehyde groups formed. The structure of OTr was characterized by FTIR, 13C NMR, MALDI-TOF mass, and the reactionprocess of OTr was analyzed. The anti-crease property, stiffness, whiteness, tensile strength, hydrophilicity and stainingproperty of fabrics treated with OTr was compared to conventional anti-crease finishing reagents. Results show that the anticreaseproperty and stiffness of the OTr-treated fabric were significantly improved. The crease recovery angle reached 254 o. The whiteness, tensile strength, hydrophilicity and staining of the fabric remained closer to that of the non-treated fabric, ascompared to other anti-crease treatments.

Bio-Inspired Coloring and Functionalization of Silk Fabric via Laccase-Catalyzed Graft Polymerization of Arylamines

Yelin Nong,Zhou Zhou,Jiugang Yuan,Ping Wang,Yuanyuan Yu,Qiang Wang,Xuerong Fan 한국섬유공학회 2020 Fibers and polymers Vol.21 No.9

Generally, dyeing and finishing of Bombyx mori silk fibers were proceeded at high temperature, which has thedisadvantages of overmuch energy-consumptions. In the present work, bio-inspired in situ coloring and functionalization ofsilk fabric were carried out via laccase-catalyzed graft polymerization of arylamines, including aniline (ANI) and pphenylenediamine(PPD). p-Hydroxyphenylacetamide (PHAD) as the model compound of tyrosine residues in silk fibroin(SF) was used to explore the mechanism of graft polymerization by means of MALDI-TOF MS. The actions of the dopantsof sodium dodecyl benzene sulfonate (SDBS) and sodium p-aminobenzenesulfonate (ABSA) on the enzymatic treatmentwere also concerned. The results indicated that laccase might initiate the self-polymerizations of ANI and PPD, respectively,and formed the copolymers of arylamine and PHAD subsequently. High color depth and color fastness were obtained afterincubation silk fabric with laccase and arylamines, companying with a noticeable pH-responsiveness. The laccase-mediatedtreatment endowed silk fabric with antibacterial and antioxidant abilities, meanwhile, the addition of dopants of SDBS andABSA promoted the enzymatic finishing. Enzymatic graft polymerization of arylamine onto silk fibroin provides a novelapproach for dyeing and functionalization of silk fabrics under mild treating conditions.

Eco-friendly Grafting of Chitosan as a Biopolymer onto Wool Fabrics Using Horseradish Peroxidase

Lingling Xu,Nan Zhang,Qiang Wang,Jiugang Yuan,Yuanyuan Yu,Ping Wang,Xuerong Fan 한국섬유공학회 2019 Fibers and polymers Vol.20 No.2

Chitosan and enzymes have been extensively used in modification of wool, due to their non-toxic and eco-friendly characteristics, as well as to reduce pollution in textile production. In the present work, chitosan was grafted onto wool fabrics using horseradish peroxidase (HRP) in order to endow wool with outstanding properties. The reaction mechanism, grafting percentage, and properties of chitosan-grafted pretreated wool fabric including wettability, dyeability, shrink resistance, and antibacterial activity were studied. P-hydroxyphenylacetamide (PHAD), as a model compound for tyrosine residues in wool,was used to study the mechanism of HRP-mediated grafting of chitosan onto wool. UV and FTIR analyses indicated that quinones, which are intermediate of HRP/H2O2-catalyzed PHAD, reacted with the amino groups of chitosan by Schiff base or Michael addition reactions. Scanning electron microscopy (SEM) showed that dichloroisocyanuric acid pretreatment had an etching effect and some substances were deposited onto the wool fiber. SEM and Fourier transform infrared (FTIR) spectroscopy further confirmed the covalent grafting of chitosan onto wool. Compared with chitosan-grafted unpretreatedwool, there was an obvious improvement in grafting efficiency of chitosan onto wool fabric, physical and mechanical properties after pretreatment and chitosan grafting on the wool. For chitosan-grafted pretreated wool fabrics, there was no observable change in the thermal behavior, while the hydrophilicity, dyeability, shrinkage resistance, and antibacterial activity were remarkably improved compared to that of untreated wool. The present work offers a new ecologically acceptable process of grafting chitosan on the wool.

Poly(ethylene glycol)-Templated Dyeing of Natural Phenol on Silk Fabric via Horseradish Peroxidase-Catalyzed

Na-won Baek,Xuerong Fan,Li Dai,Lin Zhang,Jiugang Yuan 한국섬유공학회 2022 Fibers and polymers Vol.23 No.12

The use of natural plants for dyeing is a new green approach in the textile industry using eco-friendly, renewable,and non-toxic reductants for dyeing fabric. Recently, various templates have been reported to enhance the polymerization ofnatural phenols. In this paper, ferulic acid (FA) was synthesized using a new approach and arranged on poly(ethylene glycol)(PEG) templates using horseradish peroxide (HRP)-catalyzed oxidative polymerization. The polymerization mechanism wasstudied in detail using high-performance liquid chromatography-electrospray ionization mass spectrometry (HPLC-ESI-MS)analysis. In addition, the template effect of PEG on 13 natural phenols was also investigated. Upon analyzing the color depth,the K/S value of the silk fabric dyed using PEG as a template was significantly higher than that in the absence of PEG. Thehigher color depth of the poly(ferulic acid)/PEG-treated silk fabrics further validated the important role of PEG as a template. This study presents an environmentally friendly method for dyeing silk fabric under mild conditions and the polymerizationmechanism. Compared to chemical polymerization reactions, this enzyme (HRP)-based synthesis is more environmentallyfriendly due to its milder reaction conditions.

A Facile and Controllable Approach for Surface Modification of Wool by Micro-dissolution

Yiyi Zhang,Nan Zhang,Qiang Wang,Yuanyuan Yu,Ping Wang,Jiugang Yuan 한국섬유공학회 2020 Fibers and polymers Vol.21 No.6

Most modifications of wool focused on its surface due to the existence of the cuticle scales. For instance,chlorination, oxidation and protease treatments were used to break down or remove the scales. However, these modificationsusually severely damaged the internal structure of wool. In this paper, a novel approach based on surface micro-dissolutionwas employed to modify the surface of wool to minimize the internal damage of wool, and improve the surface-relatedproperties of wool. The micro-dissolution system used NaHSO3/urea to dissolve the scale layer of wool. After being microdissolvedwith NaHSO3/urea system, scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS),fluorescence microscopy, Fourier transform infrared spectroscopy (FTIR), and the Allwörden reaction were utilized tocharacterize the treated wool. Furthermore, felting shrinkage, strength loss and wettability of the treated wool weredetermined. The results showed that NaHSO3/urea system could dissolve the scale layer from outside to inside. Thefluorescence microscopic images further indicated that low concentration of NaHSO3/urea system can dissolve the surface ofwool without the serious damage of internal structure. Moreover, the micro-dissolution system can be controlled to dissolvethe scale layer on the surface of wool slightly by adjusting the reaction conditions. The presented method is simple, easy tooperate, and economical, thereby can open up new directions for the surface modification of wool.

Fluorescent Sizing Agents Based on Aggregation-Induced Emission Effect for Accurate Evaluation of Permeability and Coating Property

Enqi Jin,Zhengke Wang,Manli Li,Qiaoling Hu,BEN ZHONG TANG,Jiugang Yuan 한국섬유공학회 2021 Fibers and polymers Vol.22 No.5

Warp sizing is the most important process of weaving preparation in the textile industry. Permeating and coating ofsizing paste into/on warp yarns directly determine the quality of sized yarns. However, there are many significant drawbacks,such as low accuracy and narrow variety adaptability for sizing agents and warp yarns, in the traditional determinationmethod of permeation and coating property of sizing paste. Focusing on the problem, this investigation introducedaggregation-induced emission (AIE) fluorogen to prepare a fluorescent sizing agent. The interface of sizing agent in textilecould be accurately visualized by AIE fluorogen. The three indexes to indicate the permeability and coating property ofsizing paste, i.e. permeation percentage, coating percentage and integrity percentage of sizing film, have been determinedaccurately and conveniently. This investigation efficiently solves the difficult problem of evaluating permeation and coatingproperty of sizing paste and has a significant guiding function on accurate determination of the quality of sized yarns.