A nonNMDA antagonist, GYKI 52466 improves microscopic O₂balance in the cortex during focal cerebral ischemia

Chi, Oak Z.,Chang, Qiang,Wang, Guolin,Liu, Xia,Harvey R. Weiss 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Oak Z.Chi,Qiang Chang, Guolin Wang*, Xia Liu, Harvey R. Weiss□.Deprtments of Anesthesai, Departments of Physiology and Biophysics, University of Medicne and Dentisrty of New Jersey,Robert Wood Johnson Medical School, New Brunswick, New Jersey, USA and*Department of Anesthesia, Medical University, Tianjing, People's Republic of China. A non-NMDA antagonist, GYKI 52466 improves microscopic O² balance in the cortex during focal cerebral ischemia. Proceedings of International Symposium on East-West Medicine, Seoul. 172-182, 1999.-This study was performed to test whether GYKI 52466, a non-NMDA receptor antagonist, would improve microregional oxygen supply and consumption balance in the focal cerebral ischemic area. Rats were anesthetized with 1.4% isoflurance. For the GYKI Group (n=8), 19 min before middle cerebral artery (MCA) occlusion, a bolus of 5mg/kg of GYKI 52466 iv was administered and was followed by an infusion of 5mg/kg/hr. For the control Group(n=8), the same volume of the vehicle was administered. One hour after MCA occlusion, regional cerebral blood flow (rCBF) was measured using the 14C-iodoantipyrine autoradiographic technique. Microscopic arterial and venous oxygen saturations were determined using microspectrophotometry. In the cortex contralateral to MCA occlusion, the average rCBF and the average O² consumption were lower in the GYKI Group than in the Control Group (rCBF:GYKI 65.5±24.1, Control 97.7 33.4ml/100g/min;O² consumption: GYKI3.9±1.2, Control 6.2±2.5ml O²/100g/min) without a significant difference in the number of veins with SvO²<50%. In the ischemic cortex, the number of veins with SvO²<50% was significantly smaller in the GYKI Group (21 veins out of 63)than in the Control Group(45 out of 59)without a significant difference in the average rCBF(GYKI44.9±17.7, Control 29.7±10.4) or regional O² consumption between these two groups (GYKI 3.3±1.4,Control 27.7±1.2). Our data demonstrated that GYKI 52466 was effective in improving microscopic O² balance in the focal ischemic cortical area of the brain and it decreased O² consumption in the non-ischemic cortex. [Neurological Research 1999;21:299-304]

Analysis on Early Detection of Lung Cancer by PET/CT Scan

Wang, Huo-Qiang,Zhao, Long,Zhao, Juan,Wang, Qiang Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.6

Background: This systemic analysis was conducted to to evaluate the application value of positron emission tomography/computed tomography (PET/CT) in early diagnosis of lung cancer. Methods: Clinical studies evaluating the application value of PET/CT for patients underwent PET/CT imaging. The histological diagnosis served as the standard of truth. Results: Four clinical studies which including 1330 patients with pulmonary spaceoccupying lesions were considered eligible for inclusion. Systemic analysis suggested that, in all 1330 patients, pooled sensitivity was 98.7% (1313.2/1330) and specificity was 58.2%(276.85/476). Conclusion: This systemic analysis suggests that integrated PET/CT imaging provides high sensitivity, and reasonably high specificity, and could be applied for early diagnosis of lung cancer.

Association study of vitamin E content in rice using whole genome re-sequencing

Xiao-Qiang Wang,Qiang He,Wei Tong,Byung-Kook Yun,Young-Sang Lee,Tae-Sung Kim,Chang-Yong Lee,Yong-Jin Park 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

Rice is the major food for half of the world population. The nutrition component in rice is critical for improvement of people’s health. Vitamin E serves as important antioxidant by quenching the free radical intermediates and thus protects the cell membrane. Because of the high nutritional value and the benefits of vitamin E in human health, increasing the tocochromanol content of major agricultural crops has long been in the focus of breeding programs and genetic engineering approaches. The key genes involved in tocopherol biosynthesis have been elucidated in Arabidopsis and other model organisms. Quantitative trait locus (QTL) study performed in Arabidopsis suggested that some of these key genes and a few additional loci contribute to natural tocopherol variations. Identifying such genetic variations in rice, enrich our understanding of the genetic mechanisms controlling tocopherol variation, which can be directly applied to rice breeding programs. In this study, we used genome-wide association mapping with high-resolution density SNPs of rice core set to identify natural allelic variations, which contribute to tocopherol increase in rice

A chloroplast variation map generated using whole genome re-sequencing of Korean landrace rice reveals phylogenetic relationships among Oryza sativa subspecies

Wei Tong,Qiang He,Xiao-Qiang Wang,Min-Young Yoon,Won-Hee Ra,Feng Peng Li,Jie Yu,Win Htet Oo,Sun-Kyung Min,Buung Choi,Eun-Beom Heo,Byoung-Kook Yun,Kyu-Won Kim,Tae-Sung Kim,Chang-Yong Lee,Yong-Jin Park 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

Although the overall structure of the chloroplast genome is generally conserved, a number of sequence variations have been identified, which are valuable for plant population and evolutionary studies. Here, we constructed a chloroplast variation map of 30 landrace rice strains of Korean origin, using the Oryza rufipogon chloroplast genome (Genbank: NC_017835) as a reference. Differential distribution of single nucleotide polymorphisms (SNPs) and indels across the rice chloroplast genome is suggestive of a region-specific variation. Population structure clustering revealed the existence of two clear subgroups (indica and japonica) and an admixture group (aus). Phylogenetic analysis of the 30 landrace rice strains and six rice chloroplast references suggested and supported independent evolution of O. sativa indica and japonica. Interestingly, two “aus” type accessions, which were thought to be indica type, shared a closer relationship with the japonica type. One hypothesis is that “Korean aus” was intentionally introduced and may have obtained japonica chloroplasts during cultivation. We also calculated the nucleotide diversity of 30 accessions and compared to six rice chloroplast references, which shown a higher diversity in the indica and aus groups than in the japonica group in lower level substitution diversity.

Galectin-9 Acts as a Prognostic Factor with Antimetastatic Potential in Hepatocellular Carcinoma

Zhang, Zhao-Yang,Dong, Jia-Hong,Chen, Yong-Wei,Wang, Xian-Qiang,Li, Chong-Hui,Wang, Jian,Wang, Guo-Qiang,Li, Hai-Lin,Wang, Xue-Dong Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.6

Considerable research has been conducted concerning galectin-9 and carcinomas, but little information is available about any relation with the hepatocellular carcinoma. In this study, we employed a small interfering RNA (siRNA) targeting galectin-9 to down-regulate the expression in HepG2 cells. As a result, after galectin-9 expression was reduced, cell aggregation was suppressed, while other behaviour such as the proliferation, adhesion and invasion to ECM, cell-endothelial adhesion and transendothelial invasion of the cells were markedly enhanced. When tumors of 200 patients with hepatocellular carcinoma were tested for galectin-9 expression by immunohistochemistry, binding levels demonstrated intimate correlations with the histopathologic grade, lymph node metastasis, vascular invasion and intrahepatic metastasis (P<0.05). Moreover, survival analysis indicated that patients with galectin-9 expression had much longer survival time than those with negative lesions, and the Log-rank test indicated that this difference was statistical significant (P<0.0001). The Cox proportional hazards model suggested that negative galectin-9 expression in hepatocellular carcinoma represented a significant risk factor for patient survival. We propose that galectin-9 might be a new prognostic factor with antimetastatic potential in patients with hepatocellular carcinoma.

On time reversal-based signal enhancement for active lamb wave-based damage identification

Qiang Wang,Shenfang Yuan,Ming Hong,Zhongqing Su 국제구조공학회 2015 Smart Structures and Systems, An International Jou Vol.15 No.6

Lamb waves have been a promising candidate for quantitative damage identification for various engineering structures, taking advantage of their superb capabilities of traveling for long distances with fast propagation and low attenuation. However, the application of Lamb waves in damage identification so far has been hampered by the fact that the characteristic signals associated with defects are generally weaker compared with those arising from boundary reflections, mode conversions and environmental noises, making it a tough task to achieve satisfactory damage identification from the time series. With awareness of this challenge, this paper proposes a time reversal-based technique to enhance the strength of damage-scattered signals, which has been previously applied to bulk wave-based damage detection successfully. The investigation includes (i) an analysis of Lamb wave propagation in a plate, generated by PZT patches mounted on the structure; (ii) an introduction of the time reversal theory dedicated for waveform reconstruction with a narrow-band input; (iii) a process of enhancing damage-scattered signals based on time reversal focalization; and (iv) the experimental investigation of the proposed approach to enhance the damage identification on a composite plate. The results have demonstrated that signals scattered by delamination in the composite plate can be enhanced remarkably with the assistance of the proposed process, benefiting from which the damage in the plate is identified with ease and high precision.

Expression of MiR200a, miR93, Metastasis-related Gene RECK and MMP2/MMP9 in Human Cervical Carcinoma - Relationship with Prognosis

Wang, Ling,Wang, Qiang,Li, He-Lian,Han, Li-Ying Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3

Aim and Background: Cervical cancer remains the third most common cancer in women globally after breast and colorectal cancer. Well-characterized biomarkers are necessary for early diagnosis and to predict metastatic progression and effective therapy. MiRNAs can regulate gene expression, cell growth, differentiation and apoptosis by targeting mRNAs for translational repression or degradation in tumor cells. The present study was conducted to assess expression of miR93, miR200a, RECK, MMP2, MMP9 in invasive cervical carcinoma, and analyze their clinical significance. Method: A total of 116 patients with invasive cervical carcinoma and 100 patients undergoing hysterectomy for benign lesions were retrospectively examined. Quantitative real-time PCR was performed to determine expression of miR93 and miR200a while RECK, MMP2, MMP9 and MVD were assessed by immunohistochemical staining. Results: Cervical carcinoma patients demonstrated up-regulation of miR-93, miR-200a, MMP2 and MMP9, with down-regulation of RECK as compared to benign lesion tissues. RECK was significantly inversely related to invasion and lymphatic metastasis. The 5-year survival rate for patients with strong RECK expression was significantly higher than that with weakly expressing tumors. Conclusion: MiR-93 and miR-200a are associated with metastasis and invasion of cervical carcinoma. Thus together with RECK they are potential prognostic markers for cervical carcinoma. RECK cooperating with MMP2, MMP9 expression is a significant prognostic factor correlated with long-term survival for patients with invasive cervical carcinoma.

A simple approach to the elasto-plastic coupling analyses of circular tunnels in confining pressure-dependent strain-softening rock masses

Qiang Zhang,Cheng Li,Qiang Guo,Ming Min,Yanning Wang,Binsong Jiang 한국자원공학회 2017 Geosystem engineering Vol.20 No.5

The conventional strain-softening behaviors which assume the strength parameters as the functions of the plastic strain have been studied using various methods. However, rock-like materials have also been found to manifest pressure-dependent behavior for both elastic and plastic rock masses. The realization of the radial stress was found to be continuous, and gradually increased from supporting stress to in situ stress for the circular openings under hydrostatic pressure. The radial stress was spaced as numbers of sections, and each annulus was considered as an ideal plastic rock mass. The close-formed analytical solutions of each annulus can be easily obtained. Therefore, using the displacement continuum and stress boundary conditions, the dimensionless radius corresponding to the spaced radius could be derived. The radial stress and plastic shear strain at the inner radius of the outer adjacent annulus were employed to describe the material property evolution law. Then, the radii could be recursively obtained. Finally, for the conventional strain-softening rock masses, the pressure-dependent elastic rock and elasto-plastic coupling strain-softening rock masses were employed to validate the proposed approach. In this study, having completed the above steps, it was concluded that the results were in accordance with the numerical methods.

Enhancement of Cellulase Efficiency on Hardwood Dissolving Pulp by Cationic Polyacrylamide Treatment

Qiang Wang,Tianzhong Yuan,Guihua Yang,Xingxiang Ji,Gaojin Lyu,Jiachuan Chen 한국펄프·종이공학회 2016 한국펄프·종이공학회 학술발표논문집 Vol.2016 No.10

Theoretical Support and Research Trend of Senior Tourism Research

Qiang Wang 융합관광콘텐츠학회 2023 융합관광콘텐츠학회 학술대회 발표집 Vol.2023 No.7