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Interleukin-20 targets podocytes and is upregulated in experimental murine diabetic nephropathy
Yu-Hsiang Hsu,Hsing-Hui Li,Junne-Ming Sung,Wei-Yu Chen,Ya-Chin Hou,Yun-Han Weng,Wei-Ting Lai,Chih-Hsing Wu,Ming-Shi Chang 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-
Interleukin (IL)-20, a proinflammatory cytokine of the IL-10 family, is involved in acute and chronic renal failure. The aim of this study was to elucidate the role of IL-20 during diabetic nephropathy development. We found that IL-20 and its receptor IL-20R1 were upregulated in the kidneys of mice and rats with STZ-induced diabetes. In vitro, IL-20 induced MMP-9, MCP-1, TGF-β1 and VEGF expression in podocytes. IL-20 was upregulated by hydrogen peroxide, high-dose glucose and TGF-β1. In addition, IL-20 induced apoptosis in podocytes by activating caspase-8. In STZ-induced early diabetic nephropathy, IL-20R1-deficient mice had lower blood glucose and serum BUN levels and a smaller glomerular area than did wild-type controls. Anti-IL-20 monoclonal antibody (7E) treatment reduced blood glucose and the glomerular area and improved renal functions in mice in the early stage of STZ-induced diabetic nephropathy. ELISA showed that the serum IL-20 level was higher in patients with diabetes mellitus than in healthy controls. The findings of this study suggest that IL-20 induces cell apoptosis of podocytes and plays a role in the pathogenesis of early diabetic nephropathy.
Use of Lactobacillus Brevis LUC247 to Enrich Quinoa Sourdough with Γ-Aminobutyric Acid
( Ting Wei Liu ),( Chao Feng Yu ),( Syue Fong Lai ),( Ying Chen Lu ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1
γ-Aminobutyric acid is a neurotransmitter inhibitor with sedation, anti-depression, hypotensive and other physiological functions. This study aimed at investigating the effect of addition various amount of quinoa (Chenopodium quinoa) flours to sourdough in γ-aminobutyric acid production. Type I sourdough containing quinoa flours of different content (0%, 15%, 30%, 45%) were prepared in laboratory. Lactobacillus brevis LUC247 was used to ferment quinoa sourdough during different fermentation time (0, 2, 4, 6, 8, 12, 24, 36, 48 hours). Compared to control dough, without quinoa, the amount of GABA was representing about 3-fold increase, Cell count of Lactobacillus brevis LUC247 in quinoa sourdough fermented for 12 hours was the highest (1E+9 CFU/g). The higher the inoculated cell counts, the faster the pH decreased and total titratable acidity and organic acid (lactic acid and acetic acid) amounts increased in quinoa sourdough. The results indicated the potential of quinoa flour through sourdough fermentation by Lactobacillus brevis LUC247 to enrich γ-aminobutyric acid.
The effects of grooves on wind characteristics of tall cylinder buildings
Wei-bin Yuan,Nan-ting Yu,Zhao Wang 한국풍공학회 2018 Wind and Structures, An International Journal (WAS Vol.26 No.2
For most full-scale tall buildings the Reynolds number of a flow field around a circular cylinder under strong wind is usually greater than 2107, which is difficult to achieve in most wind tunnel tests. To explore the wind characteristics of tall cylindrical buildings with equidirectional grooves from subcritical to transcritical flow (6.6104 Re 3.3105 and 9.9106 Re 7.2107), wind tunnel tests and full-scale large eddy simulations were carried out. The results showed that the rectangular-grooves narrow the wake width due to the downstream movement of the separation point and the deeper grooves cause smaller mean and fluctuating pressure while the peak pressure is little affected. Furthermore, the grooves lead to lower frequency of vortex shedding but the Strouhal number remains at the range from 0.15 to 0.35. The drag coefficient of the cylinders with grooves was found to be 2~3 times as large as that of smooth cylinders.
Ting-Yan Shi,Sheng Yin,Jianqing Zhu,Ping Zhang,Jihong Liu,Libing Xiang,Yaping Zhu,Sufang Wu,Xiaojun Chen,Xipeng Wang,Yin-Cheng Teng,Tao Zhu,Aijun Yu,Yingli Zhang,Yanling Feng,He Huang,Wei Bao,Yanli Li 대한부인종양학회 2020 Journal of Gynecologic Oncology Vol.31 No.3
Background: In China, secondary cytoreductive surgery (SCR) has been widely used in ovarian cancer (OC) over the past two decades. Although Gynecologic Oncology Group-0213 trial did not show its overall survival benefit in first relapsed patients, the questions on patient selection and effect of subsequent targeting therapy are still open. The preliminary data from our pre-SOC1 phase II study showed that selected patients with second relapse who never received SCR at recurrence may still benefit from surgery. Moreover, poly(ADP-ribose) polymerase inhibitors (PARPi) maintenance now has been a standard care for platinum sensitive relapsed OC. To our knowledge, no published or ongoing trial is trying to answer the question if patient can benefit from a potentially complete resection combined with PARPi maintenance in OC patients with secondary recurrence. Methods: SOC-3 is a multi-center, open, randomized, controlled, phase II trial of SCR followed by chemotherapy and niraparib maintenance vs chemotherapy and niraparib maintenance in patients with platinum-sensitive second relapsed OC who never received SCR at recurrence. To guarantee surgical quality, if the sites had no experience of participating in any OC-related surgical trials, the number of recurrent lesions evaluated by central-reviewed positron emission tomography–computed tomography image shouldn't be more than 3. Eligible patients are randomly assigned in a 1:1 ratio to receive either SCR followed by 6 cycles of platinum-based chemotherapy and niraparib maintenance or 6 cycles of platinum-based chemotherapy and niraparib maintenance alone. Patients who undergo at least 4 cycles of chemotherapy and must be, in the opinion of the investigator, without disease progression, will be assigned niraparib maintenance. Major inclusion criteria are secondary relapsed OC with a platinum-free interval of no less than 6 months and a possibly complete resection. Major exclusion criteria are borderline tumors and non-epithelial ovarian malignancies, received debulking surgery at recurrence and impossible to complete resection. The sample size is 96 patients. Primary endpoint is 12-month non-progression rate. Trial Registration: ClinicalTrials.gov Identifier: NCT03983226
Yu-Jia Lin,Hsiao-Ting Chang,Ming-Hwai Lin,Ru-Yih Chen,Ping-Jen Chen,Wen-Yuan Lin,Jyh-Gang Hsieh,Ying-Wei Wang,Chung-Chieh Hu,Yi-Sheng Liou,Tai-Yuan Chiu,Chun-Yi Tu,Yi-Jen Wang,Bo-Ren Cheng,Tzeng-Ji Ch 한국한의학연구원 2021 Integrative Medicine Research Vol.10 No.2
Background: Medical staff may have difficulties in using conventional medicine to manage symptoms among terminally ill patients, including adverse effects of the treatment. Traditional Chinese medicine (TCM) is regarded as a complementary or alternative medicine, and has been increasingly used in the field of palliative medicine in recent years. This study aimed to investigate the experiences of and attitudes toward using TCM among palliative care professionals, and to provide preliminary information about its use in palliative care. Methods: This was a cross-sectional survey study conducted in eight inpatient hospice wards in Taiwan between December 2014 and February 2016. The questionnaire was self-administered, and was analyzed with descriptive statistics including Pearson’s Chi-square test and Fisher’s exact test. Results: A total of 251 palliative care professionals responded to the questionnaire, of whom 89.7% and 88.9% believed that the use of TCM could improve the physical symptoms and quality of life in terminally ill patients, respectively. Overall, 59.8%, of respondents suggested that TCM had rare side effects, and 58.2% were worried that TCM could affect the liver and kidney function of patients. In total, 89.7% and 88.0% of professionals agreed there were no suitable clinical practice guidelines and educational programs, respectively, for TCM use in palliative care. Conclusions: Most of the respondents agreed there was insufficient knowledge, skills-training, and continuing education on the use of TCM in terminally ill patients in Taiwan. These results show that to address patient safety considerations, guidelines about use of TCM in palliative care should be established.
Deflection of Castellated Beams Subjected to Uniformly Distributed Transverse Loading
Wei-bin Yuan,Nan-ting Yu,Zhao-shui Bao,Li-ping Wu 한국강구조학회 2016 International Journal of Steel Structures Vol.16 No.3
This paper presents an analytical solution for determining the deflection of castellated/cellular beams with hexagonal/circular web openings, subjected to a uniformly distributed transverse load. The solution is derived using the principle of minimum potential energy. To validate the derived analytical solution, three-dimensional linear finite element analysis is performed using four-node shell elements built-in ANSYS software. Good agreement between the finite element result and the present analytical solution is demonstrated.
Sequencial Enzymatic Synthesis of Sugar nucleotides and Poly-LacNAc
Wei-Ting Chien,Ching-Ching Yu,Chun-Cheng Lin 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1
Poly-N-acetyllactosamine (poly-LacNAc, (3Gal1-4GlcNAc1-)n) is a linear carbohydrate polymer composed by repeatingN-acetylglucosamine (GlcNAc) and galactose (Gal) residues. These polysaccharides are found either on N- / O-linked glycoproteins or glycolipids and involve in diverse cellular functions such as differentiation, apoptosis, and metastasis. Poly-LacNAc can be further modified by various glycosyltransferases to create branched structures and display terminal epitopes. Sialylated and fucosylated derivatives of poly-LacNAc have been characterized as specific ligands for different lectins such as selectins and galectins as well as being tumor-associated antigens.It is demanded to have various lengths of oligo-LacNAc for studying LacNAc associated biology. However, the traditional organic synthesis requires tedious multiple protection and de-protection steps which are heavily relied on labor-intensive and time-consuming synthetic routes. To efficiently and quickly produce oligo-LacNAc, we expressed the recombinant bacterial enzymes, □ - 1,3-N-acetyl-glucosaminyltransferase of Helicobacter pylori (HpGnT) and □ -1,4-galactosyltransferase of Neisseria meningitides (NmGalT), from E. coli. Defined lengths of oligo-LacNAcs were synthesized by using the expressed enzymes in the presence of sugar donors, uridine 5’-diphosphate galactose (UDP- Gal) and uridine 5’-diphosphate N-acetylglucosamine (UDP-GlcNAc). To reduce the cost of NDP-sugars used in the oligo-LacNAc synthesis, we set up an enzymatic system for one-pot synthesis of NDP- sugars by using a wild-type bacterial thymidylyltransferase (RmlA). In this study, we applied RmlA to synthesize both UDP-Gal and UDP-GlcNAc. In combination with the use of corresponding kinases, UDP- GlcNAc and UDP-Gal were obtained from cheap starting materials, GlcNAc and Gal. Take the advantage of the great thermal stability of RmlA, the sugar nucleotides were prepared with quantitative yield at 55 oC within two hours. By the alternative addition of HpGnT and NmGalT, different lengths of oligo-LacNAc were synthesized. Compare to previous report, we successfully achieve the synthesis of oligo-LacNAc with a more economical way.