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장내의 탄수화물과 지방 흡수 억제를 통한 체지방 및 비만 개선 효과에 관한 연구
정은희,윤승원,이홍석,윤유식,유경미,황인경 한국조리과학회 2003 한국식품조리과학회지 Vol.19 No.1
In a previous study, a dietary supplement was developed in our lab using natural herbal extracts against digest enzyme activity in GI tract for weight control. This natural herbal extracts could regulate absorption of glucose and lipid by the inhibition of digest enzyme activity. In this study, we screened the natural herbs that inhibit glucoamylase activity and developed an water extract of cinnamon. The cinnamon extract delayed and decreased the increment of carbohydrate degradation through the inhibition of glucoamylase activity in vitro. Fifty volunteers were subjected to the intake of the herbal extracts by taking twice a day for 60 days. As a result, the treated subjects lost 3kg of body weight and 3.5kg of body fat mass after the treatment. Furthermore, the body mass index and waist size were significantly decreased during the experimental period. Above results suggested that the administration of the dietary additives composed of cinnamon and natural herbal extract improves the obesity by the decrement of body weight and body fat mass.
The Smurf ubiquitin ligases regulate tissue separation via antagonistic interactions with ephrinB1
Hwang, Yoo-Seok,Lee, Hyun-Shik,Kamata, Teddy,Mood, Kathleen,Cho, Hee Jun,Winterbottom, Emily,Ji, Yon Ju,Singh, Arvinder,Daar, Ira O. Cold Spring Harbor Laboratory Press 2013 Genes & development Vol.27 No.5
<P>Smad ubiquitin regulatory factors (Smurfs) play important roles in cell growth and differentiation. Hwang et al. present a new mechanism of Smurf regulation in <I>Xenopus</I> with evidence that Smurf1 and Smurf2 essentially compete for association with ephrin B1 (Smurf2 triggers ephrinB1 degradation, and Smurf1 counteracts it) and so regulate its expression. This unique mechanism for ubiquitin ligases is responsible for regulating the tissue boundaries at the mesoderm/ectoderm border during embryogenesis.</P>
Antimorphic PV.1 Causes Secondary Axis by Inducing Ectopic Organizer
Hwang, Yoo-Seok,Seo, Jeong-Jae,Cha, Sang-Wook,Lee, Hyun-Shik,Lee, Sung-Young,Roh, Dong-Hyun,Kung, Hsiang-fu,Kim, Jaebong,Park, Mae Ja 경북대학교 병원 2002 경북대학교병원의학연구소논문집 Vol.6 No.1
Xenopus homeobox gene, PV.1 ventralizes activininduceddorsal mesoderm and inhibits neuralization of ectoderm in animal cap when overexpressed. Here we generated PV.1/engrailed fusion construct (N-PV1-EnR) to perform loss-of-function study for this transcription factor.N-PV1-EnR showed an extremely antimorphic effect, causing a partial secondary embryonic axis when expressed at ventral marginal zone of blastula. In ventral marginal zone cells, this chimeric protein induced organizer genes and suppressed ventral markers mimicking those effects reported for dominant negative BMP-4 receptor (DNBR).Moreover, N-PV1-EnR rescued the ventralized embryos caused by the ectopic dorsal expression of PV.1 but not by that of Xvent-2. These results suggested that PV.1 functions at downstream of BMP-4 as a ventralizing effector which acts separately from Xvent-2 and the dominant negative effect gained by this specific mutant is applicable for the further studiesof BMP-4 downstream pathway.
BMP 신호 하부 인자인 PV.1 단백질의 결합 단백질 동정
황유석(Yoo-Seok Hwang),채정필(Jeong-Pil Chae),김동선(Dong-Sun Kim),박권무(Kwon Moo Park),배용철(Yong Chul Bae),박매자(Mae Ja Park) 대한해부학회 2007 Anatomy & Cell Biology Vol.40 No.3
척추동물에서 BMP의 배(ventral)쪽 신호 전달 경로의 하위에 있는 전사조절인자들은 등배(dorsalventral)쪽 축을 따라 형성되는 중배엽에 있어서도 그 기능을 공유 한다는 것이 보고 되었다. 본 연구에서는 BMP 신호 물질의 하위 유전자인 PV.1 단백질이 배쪽과 등쪽에서의 신호전달에 있어서 차별적 기능을 알기 위하여 PV.1과 상호 결합하는 단백질을 검색하였다. 효모 이중 접합법을 이용하여 24개의 PV.1 결합 단백질을 검색하였고, 이중 결합하는 Xvent-2와 Xclaudin-6에 대한 PV.1 단백질의 도메인(domain) 연구를 하였다. PV.1 단백질의 C-terminus인 197-241 지역이 Xclaudin-6와 결합하는 반면에 Xvent-2는 PV.1 단백질의 C-terminus 전반에서 결합한다는 것을 확인하였다. 또한 PV.1과 결합하는 Xvent-2는 동형 이중 결합(Homodimerization)뿐만 아니라 PV.1과 결합하는 Xclaudin-6과도 이형 이중 결합(Heteodimerization)을 한다는 것을 확인하였다. Homeodomain transcription factors functioning downstream of BMP ventral pathway have been reported to share similar domain of roles in mesoderm patterning along the dorsal-ventral axis. To elucidate the differential role of PV.1 in the aspect of relationship between dorsal and ventral region, we tried to screen PV.1- interacting proteins. Twenty-four PV.1-interacting proteins were identified by yeast two-hybrid screening. Xvent-2 and Xclaudin-6 among these, went under domain study. The C-terminus of PV.1, more specifically 197-241 region was found to interact with Xclaudin-6. Meanwhile Xvent-2 has mild affinity to overall C-terminal region of PV.1. At the same time it was found that Xvent-2 homodimerizes and also binds to Xclaudin-6.
Lee, Sang Eun,Lee, Hae-Young,Cho, Hyun-Jai,Choe, Won-Seok,Kim, Hokon,Choi, Jin-Oh,Jeon, Eun-Seok,Kim, Min-Seok,Hwang, Kyung-Kuk,Chae, Shung Chull,Baek, Sang Hong,Kang, Seok-Min,Choi, Dong-Ju,Yoo, Byun American College of Cardiology 2017 JACC. Heart failure Vol.5 No.11
<P><B>Graphical abstract</B></P><P>[Figure]</P><P><B>Abstract</B></P><P><B>Objectives</B></P><P>This study aimed to assess the relationship between on-treatment blood pressure (BP) and clinical outcomes of patients with heart failure (HF).</P><P><B>Background</B></P><P>Lower BP has been reported to be related to increased mortality in various cardiovascular diseases. The optimal BP level for patients already experiencing HF is contentious.</P><P><B>Methods</B></P><P>The Korean Acute Heart Failure registry prospectively enrolled a total of 5,625 consecutive patients hospitalized for acute HF in 10 tertiary university hospitals in Korea between March 2011 and February 2014. Clinical profiles including BP were collected at admission, discharge, and during outpatient follow-up. Mean on-treatment BP was calculated from BP at discharge and at each follow-up visit. We evaluated the effects of mean on-treatment BP on the clinical outcomes of patients.</P><P><B>Results</B></P><P>Patients were followed up for a median 2.2 years. One-year mortality after discharge was 18.2%. The relationship between on-treatment BP and all-cause mortality followed a reversed J-curve relationship. A nonlinear, multivariable Cox proportional hazard model identified a nadir of systolic and diastolic BPs of 132.4/74.2 mm Hg in patients, for whom the mortality rate was lowest (p < 0.0001). The relationship with increased mortality above and below the reference BP was more definitive for diastolic BP and for HF with a preserved ejection fraction.</P><P><B>Conclusions</B></P><P>Systolic and diastolic BPs <130/70 mm Hg at discharge and during follow-up was associated with worse survival in HF patients. These data suggest that the lowest BP possible might not be an optimal target for HF patients. Further studies should establish a proper BP goal in HF patients. (Registry [Prospective Cohort] for Heart Failure in Korea [KorAHF]; NCT01389843)</P>
황윤석,이원선,백인수,유능수 江原大學校 産業技術硏究所 2009 産業技術硏究 Vol.29 No.B
The wind data measured from automated weather stations (AWS) at complex terrains in Korea was used to predict the wind velocity at nearby sites that are several kilometers away. The ten-minute averaged wind data was measured at a height of 10 meters. A commercial CFD code, WindSIM, based on the weighted averaged Navier-Stokes equation was employed. The results were compared with the data measured using meteorological masts (MM) at a height of 40 meters. The predictions using the AWS data and WindSIM showed good agreements with the measured data.
Hwang, Yoo Na,Lee, Ju Hwan,Kim, Ga Young,Shin, Eun Seok,Kim, Sung Min Elsevier 2018 Computer Methods and Programs in Biomedicine Vol.153 No.-
<P><B>Abstract</B></P> <P><B>Background and objectives</B></P> <P>The purpose of this study was to propose a hybrid ensemble classifier to characterize coronary plaque regions in intravascular ultrasound (IVUS) images.</P> <P><B>Methods</B></P> <P>Pixels were allocated to one of four tissues (fibrous tissue (FT), fibro-fatty tissue (FFT), necrotic core (NC), and dense calcium (DC)) through processes of border segmentation, feature extraction, feature selection, and classification. Grayscale IVUS images and their corresponding virtual histology images were acquired from 11 patients with known or suspected coronary artery disease using 20 MHz catheter. A total of 102 hybrid textural features including first order statistics (FOS), gray level co-occurrence matrix (GLCM), extended gray level run-length matrix (GLRLM), Laws, local binary pattern (LBP), intensity, and discrete wavelet features (DWF) were extracted from IVUS images. To select optimal feature sets, genetic algorithm was implemented. A hybrid ensemble classifier based on histogram and texture information was then used for plaque characterization in this study. The optimal feature set was used as input of this ensemble classifier. After tissue characterization, parameters including sensitivity, specificity, and accuracy were calculated to validate the proposed approach. A ten-fold cross validation approach was used to determine the statistical significance of the proposed method.</P> <P><B>Results</B></P> <P>Our experimental results showed that the proposed method had reliable performance for tissue characterization in IVUS images. The hybrid ensemble classification method outperformed other existing methods by achieving characterization accuracy of 81% for FFT and 75% for NC. In addition, this study showed that Laws features (SSV and SAV) were key indicators for coronary tissue characterization.</P> <P><B>Conclusions</B></P> <P>The proposed method had high clinical applicability for image-based tissue characterization.</P> <P><B>Highlights</B></P> <P> <UL> <LI> This study characterized coronary plaque regions in sequential IVUS image frames. </LI> <LI> A hybrid ensemble classifier was employed for plaque characterization. </LI> <LI> This method outperformed other existing methods by achieving high accuracy especially in NC and FFT. </LI> <LI> Laws features (SSV and SAV) were key indicators for coronary tissue characterization. </LI> <LI> The proposed method had great performance for tissue characterization in IVUS images. </LI> </UL> </P>
Yoo, Hyunhee,Moon, Seung-Kwan,Hwang, Taewon,Kim, Yong Seok,Kim, Joo-Hwan,Choi, Sung-Wook,Kim, Jung Hyun American Chemical Society 2013 Langmuir Vol.29 No.20
<P>This paper describes the preparation of magnetic nanoparticles modified with polyethylenimine (PEI)–folic acid (PF) conjugate and their potential biomedical applications. Magnetic nanoparticles modified with (3-(2-aminoethylamino)propyltrimethoxysilane) (AEAPS) were first prepared using a ligand exchange method to provide biocompatibility and hydrophilicity, and further conjugated with PF to carry gene and enhance specific uptake into cancer cells. We demonstrated the feasibility of the multifunctional magnetic nanoparticles as contrast agents in magnetic resonance imaging (MRI) and as gene carriers for gene delivery. In vitro results revealed that the cytotoxicity of the multifunctional magnetic nanoparticles was lower compared to that of pristine magnetic nanoparticles. Furthermore, we demonstrated the specific uptake of the magnetic nanoparticles modified with PF to KB cells using WI-38 cells as comparison by confocal microscopy. The PF-modified magnetic nanoparticles can potentially be employed as theranostic nanoplatforms for targeted gene delivery to cancer cells and simultaneous magnetic resonance imaging.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/langd5/2013/langd5.2013.29.issue-20/la3051302/production/images/medium/la-2012-051302_0008.gif'></P>