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Yi Luo,Lifei Sun,Zhen Zhu,Wei Ran,Qirong Shen 한국미생물학회 2013 The journal of microbiology Vol.51 No.3
A newly discovered alkaline antifungal protease named P6 from Bacillus subtilis N7 was purified and partially characterized. B. subtilis N7 culture filtrates were purified by 30–60% (NH4)2SO4 precipitation, anion-exchange chromatography and gel filtration chromatography. Sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) revealed a single band of 41.38 kDa. Peptide sequence of protease P6 was determined using a 4800 Plus MALDI TOF/TOFTM Analyzer System. Self-Formed Adaptor PCR (SEFA-PCR) was used to amplify the 1,149 bp open read frame of P6. Dimensional structure prediction using Automatic Modeling Mode software showed that the protease P6 consisted of two β-barrel domains. Purified P6 strongly inhibited spore and mycelium growth of Fusarium oxysporum f. sp. cucumerium (FOC) by causing hypha lysis when the concentration was 25 μg/ml. Characterization of the purified protease indicated that it had substrate specificity for gelatin and was highly active at pH 8.0–10.6 and 70°C. The P6 protease was inhibited by EDTA (2 mmol/L), phenyl methyl sulfonyl fluoride (PMSF, 1 mmol/L), Na+, Fe3+, Cu2+, Mg2+ (5 mmol/L each) and H2O2 (2%, v/v). However, protease activity was activated by Ca2+, K+, Mn2+ (5 mmol/L each), mercaptoethanol (2%, v/v) and Tween 80 (1%, v/v). In additon, activity was also affected by organic solvents such as acetone, normal butanol and ethanol, but not hexane (25%, v/v each).
Yang Sun,Zhen Qin,Jing-jing Wan,Peng-yuan Wang,Yi-Li Yang,Jian-Guang Yu,Bo-Han Hu,Ding-Feng Su,Zhu-Min Luo,Xia Liu 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-
Gender differences in fatigue manifest as females being more prone to feel exhaustion and having lower muscle endurance. However, the mechanisms of these effects remain unclear. We investigated whether orosomucoid, an endogenous anti-fatigue protein that enhances muscle endurance, is involved in this regulation. Female rats exhibited lower muscle endurance, and this gender difference disappeared in orosomucoid-1-deficient mice. Female rats also exhibited weaker orosomucoid induction in serum, liver and muscle in response to fatigue compared with male rats. Ovariectomy elevated orosomucoid levels and increased swimming time, and estrogen replenishment reversed these effects. Exogenous estrogen treatment in male and female mice produced opposite effects. Estrogen decreased orosomucoid expression and its promoter activity in C2C12 muscle and Chang liver cells in vitro, and estrogen receptor or p38 mitogen-activated protein kinase blockade abolished this effect. Therefore, estrogen negatively regulates orosomucoid expression that is responsible for the weaker muscle endurance in females.
Peng Sun,He Huang,Ding Hua Yu,Yi Hu,Zhen Chen Tang,Jiao Jiao Xia,Heng Li 한국화학공학회 2011 Korean Journal of Chemical Engineering Vol.28 No.1
Tungstophosphoric acids (PW) supported on various metal oxides (SiO_2, γ_Al_2O_3, TiO_2, ZrO_2 and CeO_2)were synthesized and used as catalysts for sorbitol dehydration to isosorbide for the first time. 30% PW/SiO_2 exhibited the best catalytic performance for sorbitol dehydration, over which 56% isosorbide selectivity could be gained at a 95% sorbitol conversion at 250℃. The catalytic performance of regenerated 30% PW/SiO_2 catalysts by dichloromethane elution showed no loss after recycling five runs. Characterizations with UV-Vis, XRD, NH3-TPD and thermal analysis techniques revealed that PW had a good dispersion, and the primary Keggin structure was preserved after supporting PW on different supports. The interaction between PW and oxides resulted in different acidity of catalysts,which affected conversion of sorbitol and selectivity for isosorbide. The final acidity order of supported PW catalysts was the following: SiO_2>γ_Al_2O_3>TiO_2 ≈ZrO_2>CeO_2.
SPEED UP EXECUTION OF PARALLEL LOOP
He, Wang Yi,Yu, Wang Zhen,Tong, Sun,Shun, Guo Fu 대한전자공학회 1992 HICEC:Harbin International Conference on Electroni Vol.1 No.1
When sequential loops are transformed into parallel loops that can be executed on distributed parallel computer system, the backward data dependence introduced by loops greatly weakens parallel degree. This paper analyzes performances of this kind of dependence and presents a loop-restructuring method to eliminate it or decrease its intensity as much as possible. In addition, the technique of reducing synchronization overhead given in 〔3〕 is spread to reduce communication overhead between iterations of parallel loops.
Heng Zhang,Qi Zhen,Jing-Qiang Cui,Huan-Wei Sun,Rang-Tong Liu,Yi-Feng Zhang,Yong Liu 한국섬유공학회 2021 Fibers and polymers Vol.22 No.1
As a stable chemical material, polypropylene (PP) micro-nanofibrous membranes are widely used in medical andhealth care. However, PP fibrous membranes with single nano scale diameter are conducive to high packing density, whichresults in large flow resistance across the membrane. Therefore, to improve the material’s water and air permeability,polyethylene glycol (PEG), an incompatible polymer with PP, was introduced by blending in a modified method using a meltblowing process. This proposed approach allows large 10 μm diameter fibers to be entangled with fine 300 nm diameterfibers to produce highly porous binary structured fibrous membranes with small pore size. Because of its binary structure, theair permeability in the material reached 569.56 mm/s and the water vertical velocity was 70.15 mm/s. Additionally, the threedimensionalnumerical calculations suggested that the high porosity and binary structure were key to enhancing waterpermeability in the fibrous membranes. These binary structured micro-nanofibrous membranes are expected to be used forfiltration, separation, health care, oil/water separation, medical wound dressings, and other applications.
Colorectal Cancer Concealment Predicts a Poor Survival: A Retrospective Study
Li, Xiao-Pan,Xie, Zhen-Yu,Fu, Yi-Fei,Yang, Chen,Hao, Li-Peng,Yang, Li-Ming,Zhang, Mei-Yu,Li, Xiao-Li,Feng, Li-Li,Yan, Bei,Sun, Qiao Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.7
Objectives: Understanding the situation of cancer awareness which doctors give to patients might lead to prognostic prediction in cases of of colorectal cancer (CRC). Methods: Subsets of 10,779 CRC patients were used to screen the risk factors from the Cancer Registry in Pudong New Area in cancer awareness, age, TNM stage, and gender. Survival of the patients was calculated by the Kaplan-Meier method and assessed by Cox regression analysis. The views of cancer awareness in doctors and patients were surveyed by telephone or household. Results: After a median observation time of 1,616 days (ranging from 0 to 4,083 days) of 10,779 available patients, 2,596 of the 4,561 patients with cancer awareness survived, whereas 2,258 of the 5,469 patients without cancer awareness and 406 of the 749 patients without information on cancer awareness died of the disease. All-cause and cancer-specific survival were poorer for the patients without cancer awareness than those with (P < 0.001 for each, log-rank test). Cox multivariate regression analysis showed that cancer concealment cases had significantly lower cancer-specific survival (hazard ratio (HR) = 1.299; 95 % confidence interval (CI): 1.200-1.407)and all-cause survival (HR = 1.324; 95 % CI: 1.227-1.428). Furthermore, attitudes of cancer awareness between doctors and patients were significantly different (P < 0.001). Conclusion: Cancer concealment, not only late-stage tumor and age, is associated with a poor survival of CRC patients.
( Shuai Zhang ),( Yong Sun ),( Zhi Qiang Yuan ),( Ying Li ),( Xiao Lu Li ),( Zhen Yu Gong ),( Yi Zhi Peng ) 생화학분자생물학회(구 한국생화학분자생물학회) 2013 BMB Reports Vol.46 No.1
Intestinal epithelial cell (IEC) apoptosis induced by hypoxia compromise intestinal epithelium barrier function. Both Akt and Hsp90 have cytoprotective function. However, the specific role of Akt and Hsp90β in IEC apoptosis induced by hypoxia has not been explored. We confirmed that hypoxia-induced apoptosis was reduced by Hsp90β overexpression but enhanced by decreasing Hsp90β expression. Hsp90β overexpression enhanced BAD phosphorylation and thus reduced mitochondrial release of cytochrome C. Reducing Hsp90β expression had opposite effects. The protective effect of Hsp90β against apoptosis was negated by LY294002, an Akt inhibitor. Further study showed that Akt phosphorylation was enhanced by Hsp90β, which was not due to the activation of upstream PI3K and PDK1 but because of stabilization of pAkt via direct interaction between Hsp90β and pAkt. These results demonstrate that Hsp90β may play a significant role in protecting IECs from hypoxia-induced apoptosis via stabilizing pAkt to phosphorylate BAD and reduce cytochrome C release. [BMB Reports 2013; 46(1): 47-52]
Lu-Lu Zhang,Yi-Yang Li,Jiang Hu,Guan-Qun Zhou,Lei Chen,Wen-Fei Li,Ai-Hua Lin,Jun Ma,Zhen-Yu Qi,Ying Sun 대한암학회 2018 Cancer Research and Treatment Vol.50 No.4
Purpose Local relapse-free survival (LRFS) differs widely among patients with T4 category nasopharyngeal carcinoma (NPC). We aimed to build a nomogram incorporating clinicopathological information to predict LRFS in T4 NPC after definitive intensity-modulated radiation therapy (IMRT). Materials and Methods Retrospective study of 415 Chinese patients with non-metastatic T4 NPC treated with definitive IMRT with or without chemotherapy at our cancer center between October 2009 and September 2013. The nomogram for LRFS at 3 and 5 years was generated based on multivariate Cox proportional hazards regression, and validated using bootstrap resampling, assessing discriminative performance using the concordance index (C-index) and determining calibration ability via calibration curves. Results Five-year LRFS was 88.8%. We identified and incorporated four independent prognostic factors for LRFS: ethmoid sinus invasion, primary gross tumor volume, age, and pretreatment body mass index. The C-index of the nomogram for local recurrence was 0.732 (95% confidence interval, 0.726 to 0.738), indicating excellent predictive accuracy. The calibration curve revealed excellent agreement between nomogram-predicted and observed LRFS probabilities. Risk subgroups based on total point score cutoff values enabled effective discrimination of LRFS. Conclusion This pretreatment nomogram enables clinicians to accurately predict LRFS in T4 NPC after definitive IMRT, and could help to facilitate personalized patient counselling and treatment strategies.